Restriction map for
pF1KB9702
(Circular) (Six-base) MAPSORT of: pF1KB9702.seq Check: 5617 from: 1 to: 4094
pF1KB9702 4094 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 347 347
Size: 4094
AccI GT'mk_AC
Cuts at: 1132 1132
Size: 4094
Acc65I G'GTAC_C
Cuts at: 782 1110 782
Size: 328 3766
AclI AA'CG_TT
Cuts at: 3934 3934
Size: 4094
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1919 2351 3098 3366 1919
Size: 432 747 268 2647
Fragments arranged by size:
2647 747 432 268
AfeI AGC'GCT
Cuts at: 863 863
Size: 4094
AflIII A'CryG_T
Cuts at: 1446 2550 3527 1446
Size: 1104 977 2013
Fragments arranged by size:
2013 1104 977
AgeI A'CCGG_T
Cuts at: 2482 2482
Size: 4094
AlwNI CAG_nnn'CTG
Cuts at: 248 475 906 1399 2966 248
Size: 227 431 493 1567 1376
Fragments arranged by size:
1567 1376 493 431 227
ApaLI G'TGCA_C
Cuts at: 440 2864 440
Size: 2424 1670
ApoI r'AATT_y
Cuts at: 1098 3447 3703 1098
Size: 2349 256 1489
Fragments arranged by size:
2349 1489 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4094
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4094
AvaI C'yCGr_G
Cuts at: 193 346 1085 1114 193
Size: 153 739 29 3173
Fragments arranged by size:
3173 739 153 29
BamHI G'GATC_C
Cuts at: 1119 1119
Size: 4094
BanI G'GyrC_C
Cuts at: 782 990 1021 1110 1524 1772 1807 782
Size: 208 31 89 414 248 35 3069
Fragments arranged by size:
3069 414 248 208 89 35 31
BanII G_rGCy'C
Cuts at: 740 1108 2138 740
Size: 368 1030 2696
Fragments arranged by size:
2696 1030 368
BbeI G_GCGC'C
Cuts at: 1025 1528 1776 1025
Size: 503 248 3343
Fragments arranged by size:
3343 503 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 411 445 720 754 411
Size: 34 275 34 3751
Fragments arranged by size:
3751 275 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1992 2753 4094 1992
Size: 761 1341 1992
Fragments arranged by size:
1992 1341 761
BclI T'GATC_A
Cuts at: 398 1614 398
Size: 1216 2878
BglII A'GATC_T
Cuts at: 596 1609 3646 596
Size: 1013 2037 1044
Fragments arranged by size:
2037 1044 1013
BlpI GC'TnA_GC
Cuts at: 1206 1206
Size: 4094
Bme1580I G_kGCm'C
Cuts at: 444 995 1719 1812 2868 444
Size: 551 724 93 1056 1670
Fragments arranged by size:
1670 1056 724 551 93
BmrI ACTGGGnnnn_n'
Cuts at: 363 1306 1387 1722 3866 363
Size: 943 81 335 2144 591
Fragments arranged by size:
2144 943 591 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2461 2461
Size: 4094
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 251 585 591 706 850 961 1000 235
Size: 16 334 6 115 144 111 39 3329
Fragments arranged by size:
3329 334 144 115 111 39 16 6
Bpu10I CC'TnA_GC
Cuts at: 476 715 476
Size: 239 3855
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1270 2149 2641 2939 1270
Size: 879 492 298 2425
Fragments arranged by size:
2425 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 975 3798 975
Size: 2823 1271
BsaAI yAC'GTr
Cuts at: 1447 1447
Size: 4094
BsaBI GATnn'nnATC
Cuts at: 6 1608 3645 6
Size: 1602 2037 455
Fragments arranged by size:
2037 1602 455
BsaHI Gr'CG_yC
Cuts at: 1022 1061 1525 1773 2475 3971 1022
Size: 39 464 248 702 1496 1145
Fragments arranged by size:
1496 1145 702 464 248 39
BsaWI w'CCGG_w
Cuts at: 1283 1804 2482 2756 2903 3314 1283
Size: 521 678 274 147 411 2063
Fragments arranged by size:
2063 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 378 595 378
Size: 217 3877
BseYI C'CCAG_C
Cuts at: 955 1519 2277 2854 3350 955
Size: 564 758 577 496 1699
Fragments arranged by size:
1699 758 577 564 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 742
Size: 4094
BsiEI CG_ry'CG
Cuts at: 104 591 821 1636 1682 2890 104
Size: 487 230 815 46 1208 1308
Fragments arranged by size:
1308 1208 815 487 230 46
BsiHKAI G_wGCw'C
Cuts at: 444 740 1108 1886 2076 2868 444
Size: 296 368 778 190 792 1670
Fragments arranged by size:
1670 792 778 368 296 190
BsmBI CGTCTCn'nnnn_
Cuts at: 280 280
Size: 4094
Bsp1286I G_dGCh'C
Cuts at: 444 740 995 1108 1719 1812 1886 2076 2138
Size: 296 255 113 611 93 74 190 62
Cuts at: 2138 2868 444
Size: 730 1670
Fragments arranged by size:
1670 730 611 296 255 190 113 93
74 62
BspEI T'CCGG_A
Cuts at: 3314 3314
Size: 4094
BspHI T'CATG_A
Cuts at: 3270 3270
Size: 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 347 759 1144 1358 1660 2041 347
Size: 412 385 214 302 381 2400
Fragments arranged by size:
2400 412 385 381 302 214
BsrBI CCG'CTC
Cuts at: 50 702 2386 2440 3924 4089 50
Size: 652 1684 54 1484 165 55
Fragments arranged by size:
1684 1484 652 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2006 2006
Size: 4094
BsrFI r'CCGG_y
Cuts at: 465 1057 2092 2273 2482 465
Size: 592 1035 181 209 2077
Fragments arranged by size:
2077 1035 592 209 181
BssHII G'CGCG_C
Cuts at: 649 959 649
Size: 310 3784
BssSI C'ACGA_G
Cuts at: 2365 2723 2365
Size: 358 3736
BstAPI GCAn_nnn'nTGC
Cuts at: 248 1471 248
Size: 1223 2871
BstBI TT'CG_AA
Cuts at: 2455 2455
Size: 4094
BstYI r'GATC_y
Cuts at: 1 596 1119 1586 1609 1944 2190 3202 3288
Size: 595 523 467 23 335 246 1012 86
Cuts at: 3288 3300 3646 1
Size: 12 346 449
Fragments arranged by size:
1012 595 523 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 298 506 948 1008 107
Size: 191 208 442 60 3193
Fragments arranged by size:
3193 442 208 191 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2044 2225 2388 2044
Size: 181 163 3750
Fragments arranged by size:
3750 181 163
BtsI GCAGTG_nn'
Cuts at: 1337 1337
Size: 4094
DraI TTT'AAA
Cuts at: 1093 1093
Size: 4094
DrdI GACnn_nn'nnGTC
Cuts at: 769 1800 2658 769
Size: 1031 858 2205
Fragments arranged by size:
2205 1031 858
EaeI y'GGCC_r
Cuts at: 497 818 1055 1679 1853 2244 2271 3616 497
Size: 321 237 624 174 391 27 1345 975
Fragments arranged by size:
1345 975 624 391 321 237 174 27
EagI C'GGCC_G
Cuts at: 818 1679 818
Size: 861 3233
EarI CTCTTCn'nnn_
Cuts at: 258 2117 2327 258
Size: 1859 210 2025
Fragments arranged by size:
2025 1859 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 856 2302 2612 2758 3904 856
Size: 1446 310 146 1146 1046
Fragments arranged by size:
1446 1146 1046 310 146
EcoICRI GAG'CTC
Cuts at: 738 1106 738
Size: 368 3726
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 251 585 591 706 850 961 1000 1919
Size: 16 334 6 115 144 111 39 919
Cuts at: 1919 2351 3098 3366 235
Size: 432 747 268 963
Fragments arranged by size:
963 919 747 432 334 268 144 115
111 39 16 6
EcoNI CCTnn'n_nnAGG
Cuts at: 175 175
Size: 4094
EcoO109I rG'GnC_Cy
Cuts at: 183 293 1233 183
Size: 110 940 3044
Fragments arranged by size:
3044 940 110
EcoRI G'AATT_C
Cuts at: 1098 1098
Size: 4094
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 4094
FspI TGC'GCA
Cuts at: 1875 1875
Size: 4094
HaeII r_GCGC'y
Cuts at: 865 937 1025 1528 1776 2798 3777 865
Size: 72 88 503 248 1022 979 1182
Fragments arranged by size:
1182 1022 979 503 248 88 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1936 1966 1968 1998 1936
Size: 30 2 30 4032
Fragments arranged by size:
4032 30 30 2
HincII GTy'rAC
Cuts at: 1133 1133
Size: 4094
KasI G'GCGC_C
Cuts at: 1021 1524 1772 1021
Size: 503 248 3343
Fragments arranged by size:
3343 503 248
KpnI G_GTAC'C
Cuts at: 786 1114 786
Size: 328 3766
MluI A'CGCG_T
Cuts at: 3527 3527
Size: 4094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 809 938 2765 2949 809
Size: 129 1827 184 1954
Fragments arranged by size:
1954 1827 184 129
MscI TGG'CCA
Cuts at: 1855 1855
Size: 4094
MslI CAynn'nnrTG
Cuts at: 2083 2210 2083
Size: 127 3967
MspA1I CmG'CkG
Cuts at: 458 508 675 684 870 950 1044 1205 1519
Size: 50 167 9 186 80 94 161 314
Cuts at: 1519 1879 2892 3137 458
Size: 360 1013 245 1415
Fragments arranged by size:
1415 1013 360 314 245 186 167 161
94 80 50 9
NaeI GCC'GGC
Cuts at: 467 1059 2275 467
Size: 592 1216 2286
Fragments arranged by size:
2286 1216 592
NarI GG'CG_CC
Cuts at: 1022 1525 1773 1022
Size: 503 248 3343
Fragments arranged by size:
3343 503 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4094
NgoMIV G'CCGG_C
Cuts at: 465 1057 2273 465
Size: 592 1216 2286
Fragments arranged by size:
2286 1216 592
NspI r_CATG'y
Cuts at: 1147 1647 2554 1147
Size: 500 907 2687
Fragments arranged by size:
2687 907 500
PciI A'CATG_T
Cuts at: 2550 2550
Size: 4094
PflMI CCAn_nnn'nTGG
Cuts at: 304 675 304
Size: 371 3723
PfoI T'CCnGG_A
Cuts at: 267 267
Size: 4094
PmeI GTTT'AAAC
Cuts at: 1093 1093
Size: 4094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3233 3265 3233
Size: 32 4062
PpuMI rG'GwC_Cy
Cuts at: 183 293 183
Size: 110 3984
PshAI GACnn'nnGTC
Cuts at: 820 820
Size: 4094
PstI C_TGCA'G
Cuts at: 344 1141 1826 344
Size: 797 685 2612
Fragments arranged by size:
2612 797 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4094
PvuII CAG'CTG
Cuts at: 675 684 1519 1879 675
Size: 9 835 360 2890
Fragments arranged by size:
2890 835 360 9
RsrII CG'GwC_CG
Cuts at: 2289 2289
Size: 4094
SacI G_AGCT'C
Cuts at: 740 1108 740
Size: 368 3726
SacII CC_GC'GG
Cuts at: 509 951 509
Size: 442 3652
SalI G'TCGA_C
Cuts at: 1131 1131
Size: 4094
SanDI GG'GwC_CC
Cuts at: 183 293 183
Size: 110 3984
SapI GCTCTTCn'nnn_
Cuts at: 258 2117 2327 258
Size: 1859 210 2025
Fragments arranged by size:
2025 1859 210
SbfI CC_TGCA'GG
Cuts at: 1141 1141
Size: 4094
ScaI AGT'ACT
Cuts at: 3611 3611
Size: 4094
SexAI A'CCwGG_T
Cuts at: 778 778
Size: 4094
SfcI C'TryA_G
Cuts at: 33 340 1137 1822 2815 3006 33
Size: 307 797 685 993 191 1121
Fragments arranged by size:
1121 993 797 685 307 191
SfoI GGC'GCC
Cuts at: 1023 1526 1774 1023
Size: 503 248 3343
Fragments arranged by size:
3343 503 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4094
SmaI CCC'GGG
Cuts at: 348 1116 348
Size: 768 3326
SmlI C'TyrA_G
Cuts at: 1249 2164 2656 2918 1249
Size: 915 492 262 2425
Fragments arranged by size:
2425 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4094
SphI G_CATG'C
Cuts at: 1147 1647 1147
Size: 500 3594
SrfI GCCC'GGGC
Cuts at: 348 348
Size: 4094
StyI C'CwwG_G
Cuts at: 107 176 500 1228 1582 107
Size: 69 324 728 354 2619
Fragments arranged by size:
2619 728 354 324 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1677 1810 2269 2478 1677
Size: 133 459 209 3293
Fragments arranged by size:
3293 459 209 133
TatI w'GTAC_w
Cuts at: 3609 3609
Size: 4094
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3611 3643 3611
Size: 32 4062
Tth111I GACn'n_nGTC
Cuts at: 1891 1891
Size: 4094
XbaI T'CTAG_A
Cuts at: 1125 1125
Size: 4094
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1589 1589
Size: 4094
XmaI C'CCGG_G
Cuts at: 346 1114 346
Size: 768 3326
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmI BsrGI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PsiI PspOMI PspXI PsrI SfiI SgrAI SnaBI
SspI StuI SwaI XhoI XmnI ZraI