Restriction map for
pF1KB9703
(Circular) (Six-base) MAPSORT of: pF1KB9703.seq Check: 7547 from: 1 to: 4097
pF1KB9703 4097 bp
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AccI GT'mk_AC
Cuts at: 1135 1135
Size: 4097
Acc65I G'GTAC_C
Cuts at: 1113 1113
Size: 4097
AclI AA'CG_TT
Cuts at: 3937 3937
Size: 4097
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1922 2354 3101 3369 1922
Size: 432 747 268 2650
Fragments arranged by size:
2650 747 432 268
AfeI AGC'GCT
Cuts at: 347 347
Size: 4097
AflIII A'CryG_T
Cuts at: 1449 2553 3530 1449
Size: 1104 977 2016
Fragments arranged by size:
2016 1104 977
AgeI A'CCGG_T
Cuts at: 2485 2485
Size: 4097
AlwNI CAG_nnn'CTG
Cuts at: 597 1402 2969 597
Size: 805 1567 1725
Fragments arranged by size:
1725 1567 805
ApaLI G'TGCA_C
Cuts at: 2867 2867
Size: 4097
ApoI r'AATT_y
Cuts at: 1101 3450 3706 1101
Size: 2349 256 1492
Fragments arranged by size:
2349 1492 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4097
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
AvaI C'yCGr_G
Cuts at: 136 363 508 846 1117 136
Size: 227 145 338 271 3116
Fragments arranged by size:
3116 338 271 227 145
BamHI G'GATC_C
Cuts at: 1122 1122
Size: 4097
BanI G'GyrC_C
Cuts at: 423 557 827 886 1113 1527 1775 1810 423
Size: 134 270 59 227 414 248 35 2710
Fragments arranged by size:
2710 414 270 248 227 134 59 35
BanII G_rGCy'C
Cuts at: 761 1111 2141 761
Size: 350 1030 2717
Fragments arranged by size:
2717 1030 350
BbeI G_GCGC'C
Cuts at: 427 561 831 1531 1779 427
Size: 134 270 700 248 2745
Fragments arranged by size:
2745 700 270 248 134
BbsI GAAGACnn'nnnn_
Cuts at: 191 191
Size: 4097
BbvCI CC'TCA_GC
Cuts at: 751 751
Size: 4097
BciVI GTATCCnnnnn_n'
Cuts at: 1995 2756 4097 1995
Size: 761 1341 1995
Fragments arranged by size:
1995 1341 761
BclI T'GATC_A
Cuts at: 1617 1617
Size: 4097
BglI GCCn_nnn'nGGC
Cuts at: 493 884 493
Size: 391 3706
BglII A'GATC_T
Cuts at: 1612 3649 1612
Size: 2037 2060
BlpI GC'TnA_GC
Cuts at: 1209 1209
Size: 4097
Bme1580I G_kGCm'C
Cuts at: 889 1722 1815 2871 889
Size: 833 93 1056 2115
Fragments arranged by size:
2115 1056 833 93
BmrI ACTGGGnnnn_n'
Cuts at: 1309 1390 1725 3869 1309
Size: 81 335 2144 1537
Fragments arranged by size:
2144 1537 335 81
BmtI G_CTAG'C
Cuts at: 346 346
Size: 4097
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 4097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 235 145
Size: 90 4007
Bpu10I CC'TnA_GC
Cuts at: 265 529 751 265
Size: 264 222 3611
Fragments arranged by size:
3611 264 222
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 958 1273 2152 2644 2942 958
Size: 315 879 492 298 2113
Fragments arranged by size:
2113 879 492 315 298
BsaI GGTCTCn'nnnn_
Cuts at: 3801 3801
Size: 4097
BsaAI yAC'GTr
Cuts at: 1450 1450
Size: 4097
BsaBI GATnn'nnATC
Cuts at: 6 1611 3648 6
Size: 1605 2037 455
Fragments arranged by size:
2037 1605 455
BsaHI Gr'CG_yC
Cuts at: 149 424 558 650 828 1528 1776 2478 3974
Size: 275 134 92 178 700 248 702 1496
Cuts at: 3974 149
Size: 272
Fragments arranged by size:
1496 702 700 275 272 248 178 134
92
BsaWI w'CCGG_w
Cuts at: 1286 1807 2485 2759 2906 3317 1286
Size: 521 678 274 147 411 2066
Fragments arranged by size:
2066 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 904 1522 2280 2857 3353 904
Size: 618 758 577 496 1648
Fragments arranged by size:
1648 758 618 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 784
Size: 4097
BsiEI CG_ry'CG
Cuts at: 104 335 1639 1685 2893 104
Size: 231 1304 46 1208 1308
Fragments arranged by size:
1308 1304 1208 231 46
BsiHKAI G_wGCw'C
Cuts at: 131 761 1111 1889 2079 2871 131
Size: 630 350 778 190 792 1357
Fragments arranged by size:
1357 792 778 630 350 190
BsmBI CGTCTCn'nnnn_
Cuts at: 409 409
Size: 4097
Bsp1286I G_dGCh'C
Cuts at: 131 761 889 1111 1722 1815 1889 2079 2141
Size: 630 128 222 611 93 74 190 62
Cuts at: 2141 2871 131
Size: 730 1357
Fragments arranged by size:
1357 730 630 611 222 190 128 93
74 62
BspEI T'CCGG_A
Cuts at: 3317 3317
Size: 4097
BspHI T'CATG_A
Cuts at: 3273 3273
Size: 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 782 1147 1361 1663 2044 782
Size: 365 214 302 381 2835
Fragments arranged by size:
2835 381 365 302 214
BsrBI CCG'CTC
Cuts at: 50 533 538 624 2389 2443 3927 4092 50
Size: 483 5 86 1765 54 1484 165 55
Fragments arranged by size:
1765 1484 483 165 86 55 54 5
BsrDI GCAATG_nn'
Cuts at: 2009 2009
Size: 4097
BsrFI r'CCGG_y
Cuts at: 560 2095 2276 2485 560
Size: 1535 181 209 2172
Fragments arranged by size:
2172 1535 209 181
BssSI C'ACGA_G
Cuts at: 280 465 760 2368 2726 280
Size: 185 295 1608 358 1651
Fragments arranged by size:
1651 1608 358 295 185
BstAPI GCAn_nnn'nTGC
Cuts at: 597 1474 597
Size: 877 3220
BstBI TT'CG_AA
Cuts at: 2458 2458
Size: 4097
BstYI r'GATC_y
Cuts at: 1 1122 1589 1612 1947 2193 3205 3291 3303
Size: 1121 467 23 335 246 1012 86 12
Cuts at: 3303 3649 1
Size: 346 449
Fragments arranged by size:
1121 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4097
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 239 2047 2228 2391 239
Size: 1808 181 163 1945
Fragments arranged by size:
1945 1808 181 163
BtsI GCAGTG_nn'
Cuts at: 1340 1340
Size: 4097
DraI TTT'AAA
Cuts at: 1096 1096
Size: 4097
DrdI GACnn_nn'nnGTC
Cuts at: 1803 2661 1803
Size: 858 3239
EaeI y'GGCC_r
Cuts at: 332 608 728 1682 1856 2247 2274 3619 332
Size: 276 120 954 174 391 27 1345 810
Fragments arranged by size:
1345 954 810 391 276 174 120 27
EagI C'GGCC_G
Cuts at: 332 1682 332
Size: 1350 2747
EarI CTCTTCn'nnn_
Cuts at: 2120 2330 2120
Size: 210 3887
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 253 598 2305 2615 2761 3907 253
Size: 345 1707 310 146 1146 443
Fragments arranged by size:
1707 1146 443 345 310 146
EcoICRI GAG'CTC
Cuts at: 759 1109 759
Size: 350 3747
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 235 1922 2354 3101 3369 145
Size: 90 1687 432 747 268 873
Fragments arranged by size:
1687 873 747 432 268 90
EcoNI CCTnn'n_nnAGG
Cuts at: 706 706
Size: 4097
EcoO109I rG'GnC_Cy
Cuts at: 141 207 369 1020 1236 141
Size: 66 162 651 216 3002
Fragments arranged by size:
3002 651 216 162 66
EcoRI G'AATT_C
Cuts at: 1101 1101
Size: 4097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 4097
FspI TGC'GCA
Cuts at: 1878 1878
Size: 4097
HaeII r_GCGC'y
Cuts at: 349 427 561 702 831 1531 1779 2801 3780
Size: 78 134 141 129 700 248 1022 979
Cuts at: 3780 349
Size: 666
Fragments arranged by size:
1022 979 700 666 248 141 134 129
78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 932 964 1072 1104 1939 1969 1971 2001 932
Size: 32 108 32 835 30 2 30 3028
Fragments arranged by size:
3028 835 108 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1136 1136
Size: 4097
KasI G'GCGC_C
Cuts at: 423 557 827 1527 1775 423
Size: 134 270 700 248 2745
Fragments arranged by size:
2745 700 270 248 134
KpnI G_GTAC'C
Cuts at: 1117 1117
Size: 4097
MluI A'CGCG_T
Cuts at: 3530 3530
Size: 4097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2768 2952 2768
Size: 184 3913
MscI TGG'CCA
Cuts at: 730 1858 730
Size: 1128 2969
MslI CAynn'nnrTG
Cuts at: 2086 2213 2086
Size: 127 3970
MspA1I CmG'CkG
Cuts at: 597 1208 1522 1882 2895 3140 597
Size: 611 314 360 1013 245 1554
Fragments arranged by size:
1554 1013 611 360 314 245
NaeI GCC'GGC
Cuts at: 562 2278 562
Size: 1716 2381
NarI GG'CG_CC
Cuts at: 424 558 828 1528 1776 424
Size: 134 270 700 248 2745
Fragments arranged by size:
2745 700 270 248 134
NcoI C'CATG_G
Cuts at: 107 107
Size: 4097
NgoMIV G'CCGG_C
Cuts at: 560 2276 560
Size: 1716 2381
NheI G'CTAG_C
Cuts at: 342 342
Size: 4097
NspI r_CATG'y
Cuts at: 636 1150 1650 2557 636
Size: 514 500 907 2176
Fragments arranged by size:
2176 907 514 500
PciI A'CATG_T
Cuts at: 2553 2553
Size: 4097
PflMI CCAn_nnn'nTGG
Cuts at: 1007 1007
Size: 4097
PmeI GTTT'AAAC
Cuts at: 1096 1096
Size: 4097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 244 276 3236 3268 244
Size: 32 2960 32 1073
Fragments arranged by size:
2960 1073 32 32
PpuMI rG'GwC_Cy
Cuts at: 207 1020 207
Size: 813 3284
PstI C_TGCA'G
Cuts at: 320 593 1144 1829 320
Size: 273 551 685 2588
Fragments arranged by size:
2588 685 551 273
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4097
PvuII CAG'CTG
Cuts at: 597 1522 1882 597
Size: 925 360 2812
Fragments arranged by size:
2812 925 360
RsrII CG'GwC_CG
Cuts at: 2292 2292
Size: 4097
SacI G_AGCT'C
Cuts at: 761 1111 761
Size: 350 3747
SalI G'TCGA_C
Cuts at: 1134 1134
Size: 4097
SanDI GG'GwC_CC
Cuts at: 207 207
Size: 4097
SapI GCTCTTCn'nnn_
Cuts at: 2120 2330 2120
Size: 210 3887
SbfI CC_TGCA'GG
Cuts at: 1144 1144
Size: 4097
ScaI AGT'ACT
Cuts at: 286 500 3614 286
Size: 214 3114 769
Fragments arranged by size:
3114 769 214
SfcI C'TryA_G
Cuts at: 33 316 589 1140 1825 2818 3009 33
Size: 283 273 551 685 993 191 1121
Fragments arranged by size:
1121 993 685 551 283 273 191
SfoI GGC'GCC
Cuts at: 425 559 829 1529 1777 425
Size: 134 270 700 248 2745
Fragments arranged by size:
2745 700 270 248 134
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
SgrAI Cr'CCGG_yG
Cuts at: 560 560
Size: 4097
SmaI CCC'GGG
Cuts at: 138 510 848 1119 138
Size: 372 338 271 3116
Fragments arranged by size:
3116 372 338 271
SmlI C'TyrA_G
Cuts at: 973 1252 2167 2659 2921 973
Size: 279 915 492 262 2149
Fragments arranged by size:
2149 915 492 279 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4097
SphI G_CATG'C
Cuts at: 636 1150 1650 636
Size: 514 500 3083
Fragments arranged by size:
3083 514 500
SrfI GCCC'GGGC
Cuts at: 848 848
Size: 4097
SspI AAT'ATT
Cuts at: 987 987
Size: 4097
StyI C'CwwG_G
Cuts at: 107 707 1231 1585 107
Size: 600 524 354 2619
Fragments arranged by size:
2619 600 524 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1680 1813 2272 2481 1680
Size: 133 459 209 3296
Fragments arranged by size:
3296 459 209 133
TatI w'GTAC_w
Cuts at: 284 498 3612 284
Size: 214 3114 769
Fragments arranged by size:
3114 769 214
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3614 3646 3614
Size: 32 4065
Tth111I GACn'n_nGTC
Cuts at: 1894 1894
Size: 4097
XbaI T'CTAG_A
Cuts at: 1128 1128
Size: 4097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1592 1592
Size: 4097
XmaI C'CCGG_G
Cuts at: 136 508 846 1117 136
Size: 372 338 271 3116
Fragments arranged by size:
3116 372 338 271
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PflMI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SrfI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BmgBI BsaXI BseRI
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI NsiI PacI PasI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SexAI
SfiI SnaBI StuI SwaI XhoI XmnI ZraI