Restriction map for
pF1KB9704
(Circular) (Six-base) MAPSORT of: pF1KB9704.seq Check: 5170 from: 1 to: 4109
pF1KB9704 4109 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 369 369
Size: 4109
AccI GT'mk_AC
Cuts at: 1147 1147
Size: 4109
Acc65I G'GTAC_C
Cuts at: 779 1125 779
Size: 346 3763
AclI AA'CG_TT
Cuts at: 3949 3949
Size: 4109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1934 2366 3113 3381 1934
Size: 432 747 268 2662
Fragments arranged by size:
2662 747 432 268
AflIII A'CryG_T
Cuts at: 1461 2565 3542 1461
Size: 1104 977 2028
Fragments arranged by size:
2028 1104 977
AgeI A'CCGG_T
Cuts at: 2497 2497
Size: 4109
AleI CACnn'nnGTG
Cuts at: 1024 1024
Size: 4109
AlwNI CAG_nnn'CTG
Cuts at: 392 1414 2981 392
Size: 1022 1567 1520
Fragments arranged by size:
1567 1520 1022
ApaI G_GGCC'C
Cuts at: 324 324
Size: 4109
ApaLI G'TGCA_C
Cuts at: 2879 2879
Size: 4109
ApoI r'AATT_y
Cuts at: 1113 3462 3718 1113
Size: 2349 256 1504
Fragments arranged by size:
2349 1504 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4109
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4109
AvaI C'yCGr_G
Cuts at: 463 616 1129 463
Size: 153 513 3443
Fragments arranged by size:
3443 513 153
BamHI G'GATC_C
Cuts at: 1134 1134
Size: 4109
BanI G'GyrC_C
Cuts at: 779 1057 1125 1539 1787 1822 779
Size: 278 68 414 248 35 3066
Fragments arranged by size:
3066 414 278 248 68 35
BanII G_rGCy'C
Cuts at: 324 965 1123 2153 324
Size: 641 158 1030 2280
Fragments arranged by size:
2280 1030 641 158
BbeI G_GCGC'C
Cuts at: 1543 1791 1543
Size: 248 3861
BbsI GAAGACnn'nnnn_
Cuts at: 739 739
Size: 4109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 927 961 927
Size: 34 4075
BciVI GTATCCnnnnn_n'
Cuts at: 2007 2768 4109 2007
Size: 761 1341 2007
Fragments arranged by size:
2007 1341 761
BclI T'GATC_A
Cuts at: 1629 1629
Size: 4109
BglI GCCn_nnn'nGGC
Cuts at: 466 662 901 466
Size: 196 239 3674
Fragments arranged by size:
3674 239 196
BglII A'GATC_T
Cuts at: 1624 3661 1624
Size: 2037 2072
BlpI GC'TnA_GC
Cuts at: 1221 1221
Size: 4109
Bme1580I G_kGCm'C
Cuts at: 324 1734 1827 2883 324
Size: 1410 93 1056 1550
Fragments arranged by size:
1550 1410 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1321 1402 1737 3881 1321
Size: 81 335 2144 1549
Fragments arranged by size:
2144 1549 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2476 2476
Size: 4109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 424 901 1065 300
Size: 124 477 164 3344
Fragments arranged by size:
3344 477 164 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 1285 2164 2656 2954 583
Size: 702 879 492 298 1738
Fragments arranged by size:
1738 879 702 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3813 3813
Size: 4109
BsaAI yAC'GTr
Cuts at: 1462 1462
Size: 4109
BsaBI GATnn'nnATC
Cuts at: 6 1623 3660 6
Size: 1617 2037 455
Fragments arranged by size:
2037 1617 455
BsaHI Gr'CG_yC
Cuts at: 1540 1788 2490 3986 1540
Size: 248 702 1496 1663
Fragments arranged by size:
1663 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1298 1819 2497 2771 2918 3329 1298
Size: 521 678 274 147 411 2078
Fragments arranged by size:
2078 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 166 196 166
Size: 30 4079
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 421 421
Size: 4109
BseYI C'CCAG_C
Cuts at: 429 805 1534 2292 2869 3365 429
Size: 376 729 758 577 496 1173
Fragments arranged by size:
1173 758 729 577 496 376
BsiEI CG_ry'CG
Cuts at: 104 654 891 1651 1697 2905 104
Size: 550 237 760 46 1208 1308
Fragments arranged by size:
1308 1208 760 550 237 46
BsiHKAI G_wGCw'C
Cuts at: 1123 1901 2091 2883 1123
Size: 778 190 792 2349
Fragments arranged by size:
2349 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 391 403 391
Size: 12 4097
Bsp1286I G_dGCh'C
Cuts at: 324 965 1123 1734 1827 1901 2091 2153 2883
Size: 641 158 611 93 74 190 62 730
Cuts at: 2883 324
Size: 1550
Fragments arranged by size:
1550 730 641 611 190 158 93 74
62
BspEI T'CCGG_A
Cuts at: 3329 3329
Size: 4109
BspHI T'CATG_A
Cuts at: 3285 3285
Size: 4109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 369 1159 1373 1675 2056 369
Size: 790 214 302 381 2422
Fragments arranged by size:
2422 790 381 302 214
BsrBI CCG'CTC
Cuts at: 50 119 182 426 753 941 2401 2455 3939
Size: 69 63 244 327 188 1460 54 1484
Cuts at: 3939 4104 50
Size: 165 55
Fragments arranged by size:
1484 1460 327 244 188 165 69 63
55 54
BsrDI GCAATG_nn'
Cuts at: 738 2021 738
Size: 1283 2826
BsrFI r'CCGG_y
Cuts at: 835 875 1054 2107 2288 2497 835
Size: 40 179 1053 181 209 2447
Fragments arranged by size:
2447 1053 209 181 179 40
BssHII G'CGCG_C
Cuts at: 504 506 508 510 1044 504
Size: 2 2 2 534 3569
Fragments arranged by size:
3569 534 2 2 2
BssSI C'ACGA_G
Cuts at: 490 2380 2738 490
Size: 1890 358 1861
Fragments arranged by size:
1890 1861 358
BstAPI GCAn_nnn'nTGC
Cuts at: 815 1486 815
Size: 671 3438
BstBI TT'CG_AA
Cuts at: 2470 2470
Size: 4109
BstEII G'GTnAC_C
Cuts at: 1074 1074
Size: 4109
BstXI CCAn_nnnn'nTGG
Cuts at: 192 931 192
Size: 739 3370
BstYI r'GATC_y
Cuts at: 1 1134 1601 1624 1959 2205 3217 3303 3315
Size: 1133 467 23 335 246 1012 86 12
Cuts at: 3315 3661 1
Size: 346 449
Fragments arranged by size:
1133 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 455 515 896 1001 107
Size: 348 60 381 105 3215
Fragments arranged by size:
3215 381 348 105 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2059 2240 2403 2059
Size: 181 163 3765
Fragments arranged by size:
3765 181 163
BtsI GCAGTG_nn'
Cuts at: 1352 1352
Size: 4109
DraI TTT'AAA
Cuts at: 1108 1108
Size: 4109
DrdI GACnn_nn'nnGTC
Cuts at: 1815 2673 1815
Size: 858 3251
EaeI y'GGCC_r
Cuts at: 383 651 902 956 1007 1694 1868 2259 2286
Size: 268 251 54 51 687 174 391 27
Cuts at: 2286 3631 383
Size: 1345 861
Fragments arranged by size:
1345 861 687 391 268 251 174 54
51 27
EagI C'GGCC_G
Cuts at: 651 1694 651
Size: 1043 3066
EarI CTCTTCn'nnn_
Cuts at: 326 2132 2342 326
Size: 1806 210 2093
Fragments arranged by size:
2093 1806 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 302 2317 2627 2773 3919 302
Size: 2015 310 146 1146 492
Fragments arranged by size:
2015 1146 492 310 146
EcoICRI GAG'CTC
Cuts at: 1121 1121
Size: 4109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 424 901 1065 1934 2366 3113 3381 300
Size: 124 477 164 869 432 747 268 1028
Fragments arranged by size:
1028 869 747 477 432 268 164 124
EcoO109I rG'GnC_Cy
Cuts at: 320 321 789 1248 320
Size: 1 468 459 3181
Fragments arranged by size:
3181 468 459 1
EcoRI G'AATT_C
Cuts at: 1113 1113
Size: 4109
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2128 2128
Size: 4109
FspI TGC'GCA
Cuts at: 688 1890 688
Size: 1202 2907
HaeII r_GCGC'y
Cuts at: 1543 1791 2813 3792 1543
Size: 248 1022 979 1860
Fragments arranged by size:
1860 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 982 1014 1951 1981 1983 2013 982
Size: 32 937 30 2 30 3078
Fragments arranged by size:
3078 937 32 30 30 2
HincII GTy'rAC
Cuts at: 1148 1148
Size: 4109
KasI G'GCGC_C
Cuts at: 1539 1787 1539
Size: 248 3861
KpnI G_GTAC'C
Cuts at: 783 1129 783
Size: 346 3763
MluI A'CGCG_T
Cuts at: 3542 3542
Size: 4109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2780 2964 2780
Size: 184 3925
MscI TGG'CCA
Cuts at: 385 1870 385
Size: 1485 2624
MslI CAynn'nnrTG
Cuts at: 1024 2098 2225 1024
Size: 1074 127 2908
Fragments arranged by size:
2908 1074 127
MspA1I CmG'CkG
Cuts at: 251 351 702 898 1220 1534 1894 2907 3152
Size: 100 351 196 322 314 360 1013 245
Cuts at: 3152 251
Size: 1208
Fragments arranged by size:
1208 1013 360 351 322 314 245 196
100
NaeI GCC'GGC
Cuts at: 2290 2290
Size: 4109
NarI GG'CG_CC
Cuts at: 1540 1788 1540
Size: 248 3861
NcoI C'CATG_G
Cuts at: 107 107
Size: 4109
NgoMIV G'CCGG_C
Cuts at: 2288 2288
Size: 4109
NotI GC'GGCC_GC
Cuts at: 651 651
Size: 4109
NspI r_CATG'y
Cuts at: 948 1162 1662 2569 948
Size: 214 500 907 2488
Fragments arranged by size:
2488 907 500 214
PciI A'CATG_T
Cuts at: 2565 2565
Size: 4109
PmeI GTTT'AAAC
Cuts at: 1108 1108
Size: 4109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3248 3280 3248
Size: 32 4077
PpuMI rG'GwC_Cy
Cuts at: 789 789
Size: 4109
PspOMI G'GGCC_C
Cuts at: 320 320
Size: 4109
PspXI vC'TCGA_Gb
Cuts at: 616 616
Size: 4109
PstI C_TGCA'G
Cuts at: 605 1156 1841 605
Size: 551 685 2873
Fragments arranged by size:
2873 685 551
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4109
PvuII CAG'CTG
Cuts at: 351 1534 1894 351
Size: 1183 360 2566
Fragments arranged by size:
2566 1183 360
RsrII CG'GwC_CG
Cuts at: 2304 2304
Size: 4109
SacI G_AGCT'C
Cuts at: 1123 1123
Size: 4109
SacII CC_GC'GG
Cuts at: 899 899
Size: 4109
SalI G'TCGA_C
Cuts at: 1146 1146
Size: 4109
SapI GCTCTTCn'nnn_
Cuts at: 2132 2342 2132
Size: 210 3899
SbfI CC_TGCA'GG
Cuts at: 1156 1156
Size: 4109
ScaI AGT'ACT
Cuts at: 3626 3626
Size: 4109
SfcI C'TryA_G
Cuts at: 33 601 784 1152 1837 2830 3021 33
Size: 568 183 368 685 993 191 1121
Fragments arranged by size:
1121 993 685 568 368 191 183
SfoI GGC'GCC
Cuts at: 1541 1789 1541
Size: 248 3861
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4109
SmaI CCC'GGG
Cuts at: 465 1131 465
Size: 666 3443
SmlI C'TyrA_G
Cuts at: 562 616 1264 2179 2671 2933 562
Size: 54 648 915 492 262 1738
Fragments arranged by size:
1738 915 648 492 262 54
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4109
SphI G_CATG'C
Cuts at: 948 1162 1662 948
Size: 214 500 3395
Fragments arranged by size:
3395 500 214
StyI C'CwwG_G
Cuts at: 107 581 1243 1597 107
Size: 474 662 354 2619
Fragments arranged by size:
2619 662 474 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 951 1692 1825 2284 2493 951
Size: 741 133 459 209 2567
Fragments arranged by size:
2567 741 459 209 133
TatI w'GTAC_w
Cuts at: 3624 3624
Size: 4109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 166 198 3626 3658 166
Size: 32 3428 32 617
Fragments arranged by size:
3428 617 32 32
Tth111I GACn'n_nGTC
Cuts at: 1906 1906
Size: 4109
XbaI T'CTAG_A
Cuts at: 1140 1140
Size: 4109
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 713 1604 713
Size: 891 3218
XhoI C'TCGA_G
Cuts at: 616 616
Size: 4109
XmaI C'CCGG_G
Cuts at: 463 1129 463
Size: 666 3443
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PciI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsgI BsiWI
BsmI BsrGI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XmnI ZraI