Restriction map for
pF1KB9707
(Circular) (Six-base) MAPSORT of: pF1KB9707.seq Check: 8652 from: 1 to: 4139
pF1KB9707 4139 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1177 1177
Size: 4139
Acc65I G'GTAC_C
Cuts at: 731 1155 731
Size: 424 3715
AclI AA'CG_TT
Cuts at: 3979 3979
Size: 4139
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1964 2396 3143 3411 1964
Size: 432 747 268 2692
Fragments arranged by size:
2692 747 432 268
AflIII A'CryG_T
Cuts at: 1055 1491 2595 3572 1055
Size: 436 1104 977 1622
Fragments arranged by size:
1622 1104 977 436
AgeI A'CCGG_T
Cuts at: 2527 2527
Size: 4139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 267 267
Size: 4139
AlwNI CAG_nnn'CTG
Cuts at: 432 1444 3011 432
Size: 1012 1567 1560
Fragments arranged by size:
1567 1560 1012
ApaI G_GGCC'C
Cuts at: 439 460 587 662 670 439
Size: 21 127 75 8 3908
Fragments arranged by size:
3908 127 75 21 8
ApaLI G'TGCA_C
Cuts at: 393 2909 393
Size: 2516 1623
ApoI r'AATT_y
Cuts at: 1143 3492 3748 1143
Size: 2349 256 1534
Fragments arranged by size:
2349 1534 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4139
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
AvaI C'yCGr_G
Cuts at: 453 693 1159 453
Size: 240 466 3433
Fragments arranged by size:
3433 466 240
BamHI G'GATC_C
Cuts at: 1164 1164
Size: 4139
BanI G'GyrC_C
Cuts at: 418 484 712 731 1155 1569 1817 1852 418
Size: 66 228 19 424 414 248 35 2705
Fragments arranged by size:
2705 424 414 248 228 66 35 19
BanII G_rGCy'C
Cuts at: 307 347 439 460 587 662 670 742 772
Size: 40 92 21 127 75 8 72 30
Cuts at: 772 1153 2183 307
Size: 381 1030 2263
Fragments arranged by size:
2263 1030 381 127 92 75 72 40
30 21 8
BbeI G_GCGC'C
Cuts at: 422 488 1573 1821 422
Size: 66 1085 248 2740
Fragments arranged by size:
2740 1085 248 66
BciVI GTATCCnnnnn_n'
Cuts at: 2037 2798 4139 2037
Size: 761 1341 2037
Fragments arranged by size:
2037 1341 761
BclI T'GATC_A
Cuts at: 1659 1659
Size: 4139
BglI GCCn_nnn'nGGC
Cuts at: 793 793
Size: 4139
BglII A'GATC_T
Cuts at: 1654 3691 1654
Size: 2037 2102
BlpI GC'TnA_GC
Cuts at: 741 991 1251 741
Size: 250 260 3629
Fragments arranged by size:
3629 260 250
Bme1580I G_kGCm'C
Cuts at: 397 439 460 587 662 670 1082 1764 1857
Size: 42 21 127 75 8 412 682 93
Cuts at: 1857 2913 397
Size: 1056 1623
Fragments arranged by size:
1623 1056 682 412 127 93 75 42
21 8
BmrI ACTGGGnnnn_n'
Cuts at: 132 309 612 1351 1432 1767 3911 132
Size: 177 303 739 81 335 2144 360
Fragments arranged by size:
2144 739 360 335 303 177 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 366 543 761 2506 366
Size: 177 218 1745 1999
Fragments arranged by size:
1999 1745 218 177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 871 137
Size: 734 3405
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1315 2194 2686 2984 1315
Size: 879 492 298 2470
Fragments arranged by size:
2470 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3843 3843
Size: 4139
BsaAI yAC'GTr
Cuts at: 1058 1492 1058
Size: 434 3705
BsaBI GATnn'nnATC
Cuts at: 6 1653 3690 6
Size: 1647 2037 455
Fragments arranged by size:
2037 1647 455
BsaHI Gr'CG_yC
Cuts at: 419 485 1570 1818 2520 4016 419
Size: 66 1085 248 702 1496 542
Fragments arranged by size:
1496 1085 702 542 248 66
BsaWI w'CCGG_w
Cuts at: 1095 1328 1849 2527 2801 2948 3359 1095
Size: 233 521 678 274 147 411 1875
Fragments arranged by size:
1875 678 521 411 274 233 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 289 319 289
Size: 30 4109
BseYI C'CCAG_C
Cuts at: 566 1081 1564 2322 2899 3395 566
Size: 515 483 758 577 496 1310
Fragments arranged by size:
1310 758 577 515 496 483
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 301
Size: 4139
BsiEI CG_ry'CG
Cuts at: 104 1681 1727 2935 104
Size: 1577 46 1208 1308
Fragments arranged by size:
1577 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 397 742 1153 1931 2121 2913 397
Size: 345 411 778 190 792 1623
Fragments arranged by size:
1623 792 778 411 345 190
Bsp1286I G_dGCh'C
Cuts at: 307 347 397 439 460 587 662 670 742
Size: 40 50 42 21 127 75 8 72
Cuts at: 742 772 1082 1153 1764 1857 1931 2121 2183
Size: 30 310 71 611 93 74 190 62
Cuts at: 2183 2913 307
Size: 730 1533
Fragments arranged by size:
1533 730 611 310 190 127 93 75
74 72 71 62 50 42 40 30
21 8
BspEI T'CCGG_A
Cuts at: 1095 3359 1095
Size: 2264 1875
BspHI T'CATG_A
Cuts at: 3315 3315
Size: 4139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1189 1403 1705 2086 1189
Size: 214 302 381 3242
Fragments arranged by size:
3242 381 302 214
BsrBI CCG'CTC
Cuts at: 50 416 1040 2431 2485 3969 4134 50
Size: 366 624 1391 54 1484 165 55
Fragments arranged by size:
1484 1391 624 366 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2051 2051
Size: 4139
BsrFI r'CCGG_y
Cuts at: 472 2137 2318 2527 472
Size: 1665 181 209 2084
Fragments arranged by size:
2084 1665 209 181
BssHII G'CGCG_C
Cuts at: 537 537
Size: 4139
BssSI C'ACGA_G
Cuts at: 528 2410 2768 528
Size: 1882 358 1899
Fragments arranged by size:
1899 1882 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1516 1516
Size: 4139
BstBI TT'CG_AA
Cuts at: 2500 2500
Size: 4139
BstXI CCAn_nnnn'nTGG
Cuts at: 936 936
Size: 4139
BstYI r'GATC_y
Cuts at: 1 1164 1631 1654 1989 2235 3247 3333 3345
Size: 1163 467 23 335 246 1012 86 12
Cuts at: 3345 3691 1
Size: 346 449
Fragments arranged by size:
1163 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 331 364 488 509 662 107
Size: 224 33 124 21 153 3584
Fragments arranged by size:
3584 224 153 124 33 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2089 2270 2433 2089
Size: 181 163 3795
Fragments arranged by size:
3795 181 163
BtsI GCAGTG_nn'
Cuts at: 615 1382 615
Size: 767 3372
DraI TTT'AAA
Cuts at: 1138 1138
Size: 4139
DraIII CAC_nnn'GTG
Cuts at: 530 530
Size: 4139
DrdI GACnn_nn'nnGTC
Cuts at: 1845 2703 1845
Size: 858 3281
EaeI y'GGCC_r
Cuts at: 1724 1898 2289 2316 3661 1724
Size: 174 391 27 1345 2202
Fragments arranged by size:
2202 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1724 1724
Size: 4139
EarI CTCTTCn'nnn_
Cuts at: 365 2162 2372 365
Size: 1797 210 2132
Fragments arranged by size:
2132 1797 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 355 1127 2347 2657 2803 3949 355
Size: 772 1220 310 146 1146 545
Fragments arranged by size:
1220 1146 772 545 310 146
EcoICRI GAG'CTC
Cuts at: 740 1151 740
Size: 411 3728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 871 1964 2396 3143 3411 137
Size: 734 1093 432 747 268 865
Fragments arranged by size:
1093 865 747 734 432 268
EcoO109I rG'GnC_Cy
Cuts at: 436 457 583 823 999 1278 436
Size: 21 126 240 176 279 3297
Fragments arranged by size:
3297 279 240 176 126 21
EcoRI G'AATT_C
Cuts at: 1143 1143
Size: 4139
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 4139
FspI TGC'GCA
Cuts at: 1033 1920 1033
Size: 887 3252
HaeII r_GCGC'y
Cuts at: 422 488 1573 1821 2843 3822 422
Size: 66 1085 248 1022 979 739
Fragments arranged by size:
1085 1022 979 739 248 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 118 150 289 321 1981 2011 2013 2043 118
Size: 32 139 32 1660 30 2 30 2214
Fragments arranged by size:
2214 1660 139 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1178 1178
Size: 4139
HindIII A'AGCT_T
Cuts at: 282 282
Size: 4139
KasI G'GCGC_C
Cuts at: 418 484 1569 1817 418
Size: 66 1085 248 2740
Fragments arranged by size:
2740 1085 248 66
KpnI G_GTAC'C
Cuts at: 735 1159 735
Size: 424 3715
MluI A'CGCG_T
Cuts at: 3572 3572
Size: 4139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 758 871 2810 2994 758
Size: 113 1939 184 1903
Fragments arranged by size:
1939 1903 184 113
MscI TGG'CCA
Cuts at: 1900 1900
Size: 4139
MslI CAynn'nnrTG
Cuts at: 707 2128 2255 707
Size: 1421 127 2591
Fragments arranged by size:
2591 1421 127
MspA1I CmG'CkG
Cuts at: 294 490 566 664 834 881 918 1250 1564
Size: 196 76 98 170 47 37 332 314
Cuts at: 1564 1924 2937 3182 294
Size: 360 1013 245 1251
Fragments arranged by size:
1251 1013 360 332 314 245 196 170
98 76 47 37
NaeI GCC'GGC
Cuts at: 474 2320 474
Size: 1846 2293
NarI GG'CG_CC
Cuts at: 419 485 1570 1818 419
Size: 66 1085 248 2740
Fragments arranged by size:
2740 1085 248 66
NcoI C'CATG_G
Cuts at: 107 331 107
Size: 224 3915
NgoMIV G'CCGG_C
Cuts at: 472 2318 472
Size: 1846 2293
NspI r_CATG'y
Cuts at: 254 706 1192 1692 2599 254
Size: 452 486 500 907 1794
Fragments arranged by size:
1794 907 500 486 452
PasI CC'CwG_GG
Cuts at: 432 432
Size: 4139
PciI A'CATG_T
Cuts at: 2595 2595
Size: 4139
PflMI CCAn_nnn'nTGG
Cuts at: 530 650 686 530
Size: 120 36 3983
Fragments arranged by size:
3983 120 36
PfoI T'CCnGG_A
Cuts at: 685 848 685
Size: 163 3976
PmeI GTTT'AAAC
Cuts at: 1138 1138
Size: 4139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3278 3310 3278
Size: 32 4107
PpuMI rG'GwC_Cy
Cuts at: 823 823
Size: 4139
PspOMI G'GGCC_C
Cuts at: 435 456 583 658 666 435
Size: 21 127 75 8 3908
Fragments arranged by size:
3908 127 75 21 8
PstI C_TGCA'G
Cuts at: 1186 1871 1186
Size: 685 3454
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4139
PvuII CAG'CTG
Cuts at: 294 566 834 881 918 1564 1924 294
Size: 272 268 47 37 646 360 2509
Fragments arranged by size:
2509 646 360 272 268 47 37
RsrII CG'GwC_CG
Cuts at: 780 2334 780
Size: 1554 2585
SacI G_AGCT'C
Cuts at: 742 1153 742
Size: 411 3728
SacII CC_GC'GG
Cuts at: 491 665 491
Size: 174 3965
SalI G'TCGA_C
Cuts at: 1176 1176
Size: 4139
SapI GCTCTTCn'nnn_
Cuts at: 365 2162 2372 365
Size: 1797 210 2132
Fragments arranged by size:
2132 1797 210
SbfI CC_TGCA'GG
Cuts at: 1186 1186
Size: 4139
ScaI AGT'ACT
Cuts at: 3656 3656
Size: 4139
SfcI C'TryA_G
Cuts at: 33 1182 1867 2860 3051 33
Size: 1149 685 993 191 1121
Fragments arranged by size:
1149 1121 993 685 191
SfoI GGC'GCC
Cuts at: 420 486 1571 1819 420
Size: 66 1085 248 2740
Fragments arranged by size:
2740 1085 248 66
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
SmaI CCC'GGG
Cuts at: 455 695 1161 455
Size: 240 466 3433
Fragments arranged by size:
3433 466 240
SmlI C'TyrA_G
Cuts at: 1294 2209 2701 2963 1294
Size: 915 492 262 2470
Fragments arranged by size:
2470 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4139
SphI G_CATG'C
Cuts at: 706 1192 1692 706
Size: 486 500 3153
Fragments arranged by size:
3153 500 486
SrfI GCCC'GGGC
Cuts at: 455 455
Size: 4139
StyI C'CwwG_G
Cuts at: 107 219 331 1273 1627 107
Size: 112 112 942 354 2619
Fragments arranged by size:
2619 942 354 112 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 335 1722 1855 2314 2523 335
Size: 1387 133 459 209 1951
Fragments arranged by size:
1951 1387 459 209 133
TatI w'GTAC_w
Cuts at: 1052 3654 1052
Size: 2602 1537
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3656 3688 3656
Size: 32 4107
Tth111I GACn'n_nGTC
Cuts at: 1936 1936
Size: 4139
XbaI T'CTAG_A
Cuts at: 1170 1170
Size: 4139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 296 329 1634 296
Size: 33 1305 2801
Fragments arranged by size:
2801 1305 33
XmaI C'CCGG_G
Cuts at: 453 693 1159 453
Size: 240 466 3433
Fragments arranged by size:
3433 466 240
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BstEII BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SspI StuI SwaI XhoI XmnI ZraI