Restriction map for
pF1KB9710
(Circular) (Six-base) MAPSORT of: pF1KB9710.seq Check: 5625 from: 1 to: 4157
pF1KB9710 4157 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 966 966
Size: 4157
AccI GT'mk_AC
Cuts at: 227 835 1195 227
Size: 608 360 3189
Fragments arranged by size:
3189 608 360
Acc65I G'GTAC_C
Cuts at: 1173 1173
Size: 4157
AclI AA'CG_TT
Cuts at: 3997 3997
Size: 4157
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 1982 2414 3161 3429 156
Size: 1826 432 747 268 884
Fragments arranged by size:
1826 884 747 432 268
AfeI AGC'GCT
Cuts at: 326 937 326
Size: 611 3546
AflIII A'CryG_T
Cuts at: 1509 2613 3590 1509
Size: 1104 977 2076
Fragments arranged by size:
2076 1104 977
AgeI A'CCGG_T
Cuts at: 2545 2545
Size: 4157
AlwNI CAG_nnn'CTG
Cuts at: 501 885 1462 3029 501
Size: 384 577 1567 1629
Fragments arranged by size:
1629 1567 577 384
ApaLI G'TGCA_C
Cuts at: 2927 2927
Size: 4157
ApoI r'AATT_y
Cuts at: 1161 3510 3766 1161
Size: 2349 256 1552
Fragments arranged by size:
2349 1552 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4157
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4157
AvaI C'yCGr_G
Cuts at: 777 1177 777
Size: 400 3757
BamHI G'GATC_C
Cuts at: 1182 1182
Size: 4157
BanI G'GyrC_C
Cuts at: 814 1173 1587 1835 1870 814
Size: 359 414 248 35 3101
Fragments arranged by size:
3101 414 359 248 35
BanII G_rGCy'C
Cuts at: 586 1058 1171 2201 586
Size: 472 113 1030 2542
Fragments arranged by size:
2542 1030 472 113
BbeI G_GCGC'C
Cuts at: 818 1591 1839 818
Size: 773 248 3136
Fragments arranged by size:
3136 773 248
BbsI GAAGACnn'nnnn_
Cuts at: 680 779 680
Size: 99 4058
BciVI GTATCCnnnnn_n'
Cuts at: 2055 2816 4157 2055
Size: 761 1341 2055
Fragments arranged by size:
2055 1341 761
BclI T'GATC_A
Cuts at: 1677 1677
Size: 4157
BglI GCCn_nnn'nGGC
Cuts at: 258 258
Size: 4157
BglII A'GATC_T
Cuts at: 1672 3709 1672
Size: 2037 2120
BlpI GC'TnA_GC
Cuts at: 1269 1269
Size: 4157
Bme1580I G_kGCm'C
Cuts at: 1782 1875 2931 1782
Size: 93 1056 3008
Fragments arranged by size:
3008 1056 93
BmgBI CAC'GTC
Cuts at: 833 1098 833
Size: 265 3892
BmrI ACTGGGnnnn_n'
Cuts at: 1369 1450 1785 3929 1369
Size: 81 335 2144 1597
Fragments arranged by size:
2144 1597 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 720 2524 720
Size: 1804 2353
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 366 730 811 907 366
Size: 364 81 96 3616
Fragments arranged by size:
3616 364 96 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1333 2212 2704 3002 1333
Size: 879 492 298 2488
Fragments arranged by size:
2488 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 790 3861 790
Size: 3071 1086
BsaAI yAC'GTr
Cuts at: 1510 1510
Size: 4157
BsaBI GATnn'nnATC
Cuts at: 6 740 1671 3708 6
Size: 734 931 2037 455
Fragments arranged by size:
2037 931 734 455
BsaHI Gr'CG_yC
Cuts at: 815 1588 1836 2538 4034 815
Size: 773 248 702 1496 938
Fragments arranged by size:
1496 938 773 702 248
BsaWI w'CCGG_w
Cuts at: 1346 1867 2545 2819 2966 3377 1346
Size: 521 678 274 147 411 2126
Fragments arranged by size:
2126 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 709 808 709
Size: 99 4058
BseYI C'CCAG_C
Cuts at: 153 208 358 455 1582 2340 2917 3413 153
Size: 55 150 97 1127 758 577 496 897
Fragments arranged by size:
1127 897 758 577 496 150 97 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 637 994 637
Size: 357 3800
BsiEI CG_ry'CG
Cuts at: 104 262 608 1699 1745 2953 104
Size: 158 346 1091 46 1208 1308
Fragments arranged by size:
1308 1208 1091 346 158 46
BsiHKAI G_wGCw'C
Cuts at: 1171 1949 2139 2931 1171
Size: 778 190 792 2397
Fragments arranged by size:
2397 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 604 604
Size: 4157
Bsp1286I G_dGCh'C
Cuts at: 586 1058 1171 1782 1875 1949 2139 2201 2931
Size: 472 113 611 93 74 190 62 730
Cuts at: 2931 586
Size: 1812
Fragments arranged by size:
1812 730 611 472 190 113 93 74
62
BspEI T'CCGG_A
Cuts at: 3377 3377
Size: 4157
BspHI T'CATG_A
Cuts at: 3333 3333
Size: 4157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 966 1094 1207 1421 1723 2104 966
Size: 128 113 214 302 381 3019
Fragments arranged by size:
3019 381 302 214 128 113
BsrBI CCG'CTC
Cuts at: 50 2449 2503 3987 4152 50
Size: 2399 54 1484 165 55
Fragments arranged by size:
2399 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1038 2069 1038
Size: 1031 3126
BsrFI r'CCGG_y
Cuts at: 232 257 261 848 2155 2336 2545 232
Size: 25 4 587 1307 181 209 1844
Fragments arranged by size:
1844 1307 587 209 181 25 4
BssSI C'ACGA_G
Cuts at: 2428 2786 2428
Size: 358 3799
BstAPI GCAn_nnn'nTGC
Cuts at: 501 1033 1534 501
Size: 532 501 3124
Fragments arranged by size:
3124 532 501
BstBI TT'CG_AA
Cuts at: 2518 2518
Size: 4157
BstYI r'GATC_y
Cuts at: 1 353 1182 1649 1672 2007 2253 3265 3351
Size: 352 829 467 23 335 246 1012 86
Cuts at: 3351 3363 3709 1
Size: 12 346 449
Fragments arranged by size:
1012 829 467 449 352 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 602 818 602
Size: 216 3941
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 731 2107 2288 2451 731
Size: 1376 181 163 2437
Fragments arranged by size:
2437 1376 181 163
BtsI GCAGTG_nn'
Cuts at: 906 1400 906
Size: 494 3663
DraI TTT'AAA
Cuts at: 1156 1156
Size: 4157
DrdI GACnn_nn'nnGTC
Cuts at: 1863 2721 1863
Size: 858 3299
EaeI y'GGCC_r
Cuts at: 216 259 422 572 605 750 1742 1916 2307
Size: 43 163 150 33 145 992 174 391
Cuts at: 2307 2334 3679 216
Size: 27 1345 694
Fragments arranged by size:
1345 992 694 391 174 163 150 145
43 33 27
EagI C'GGCC_G
Cuts at: 259 605 1742 259
Size: 346 1137 2674
Fragments arranged by size:
2674 1137 346
EarI CTCTTCn'nnn_
Cuts at: 2180 2390 2180
Size: 210 3947
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 544 873 2365 2675 2821 3967 544
Size: 329 1492 310 146 1146 734
Fragments arranged by size:
1492 1146 734 329 310 146
EcoICRI GAG'CTC
Cuts at: 1169 1169
Size: 4157
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 366 730 811 907 1982 2414 3161 3429
Size: 210 364 81 96 1075 432 747 268
Cuts at: 3429 156
Size: 884
Fragments arranged by size:
1075 884 747 432 364 268 210 96
81
EcoNI CCTnn'n_nnAGG
Cuts at: 391 391
Size: 4157
EcoO109I rG'GnC_Cy
Cuts at: 1296 1296
Size: 4157
EcoRI G'AATT_C
Cuts at: 1161 1161
Size: 4157
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2176 2176
Size: 4157
FspI TGC'GCA
Cuts at: 1938 1938
Size: 4157
HaeII r_GCGC'y
Cuts at: 150 328 406 538 818 939 1591 1839 2861
Size: 178 78 132 280 121 652 248 1022
Cuts at: 2861 3840 150
Size: 979 467
Fragments arranged by size:
1022 979 652 467 280 248 178 132
121 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 715 747 1999 2029 2031 2061 715
Size: 32 1252 30 2 30 2811
Fragments arranged by size:
2811 1252 32 30 30 2
HincII GTy'rAC
Cuts at: 1196 1196
Size: 4157
KasI G'GCGC_C
Cuts at: 814 1587 1835 814
Size: 773 248 3136
Fragments arranged by size:
3136 773 248
KpnI G_GTAC'C
Cuts at: 1177 1177
Size: 4157
MluI A'CGCG_T
Cuts at: 3590 3590
Size: 4157
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2828 3012 2828
Size: 184 3973
MscI TGG'CCA
Cuts at: 424 574 752 1918 424
Size: 150 178 1166 2663
Fragments arranged by size:
2663 1166 178 150
MslI CAynn'nnrTG
Cuts at: 247 2146 2273 247
Size: 1899 127 2131
Fragments arranged by size:
2131 1899 127
MspA1I CmG'CkG
Cuts at: 153 475 501 604 812 820 882 1268 1582
Size: 322 26 103 208 8 62 386 314
Cuts at: 1582 1942 2955 3200 153
Size: 360 1013 245 1110
Fragments arranged by size:
1110 1013 386 360 322 314 245 208
103 62 26 8
NaeI GCC'GGC
Cuts at: 2338 2338
Size: 4157
NarI GG'CG_CC
Cuts at: 815 1588 1836 815
Size: 773 248 3136
Fragments arranged by size:
3136 773 248
NgoMIV G'CCGG_C
Cuts at: 2336 2336
Size: 4157
NspI r_CATG'y
Cuts at: 1210 1710 2617 1210
Size: 500 907 2750
Fragments arranged by size:
2750 907 500
PciI A'CATG_T
Cuts at: 2613 2613
Size: 4157
PflMI CCAn_nnn'nTGG
Cuts at: 789 789
Size: 4157
PfoI T'CCnGG_A
Cuts at: 1060 1060
Size: 4157
PmeI GTTT'AAAC
Cuts at: 1156 1156
Size: 4157
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 886 918 3296 3328 886
Size: 32 2378 32 1715
Fragments arranged by size:
2378 1715 32 32
PstI C_TGCA'G
Cuts at: 320 542 1204 1889 320
Size: 222 662 685 2588
Fragments arranged by size:
2588 685 662 222
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4157
PvuII CAG'CTG
Cuts at: 882 1582 1942 882
Size: 700 360 3097
Fragments arranged by size:
3097 700 360
RsrII CG'GwC_CG
Cuts at: 2352 2352
Size: 4157
SacI G_AGCT'C
Cuts at: 1171 1171
Size: 4157
SacII CC_GC'GG
Cuts at: 605 821 605
Size: 216 3941
SalI G'TCGA_C
Cuts at: 1194 1194
Size: 4157
SapI GCTCTTCn'nnn_
Cuts at: 2180 2390 2180
Size: 210 3947
SbfI CC_TGCA'GG
Cuts at: 1204 1204
Size: 4157
ScaI AGT'ACT
Cuts at: 3674 3674
Size: 4157
SfcI C'TryA_G
Cuts at: 33 316 538 726 1200 1885 2878 3069 33
Size: 283 222 188 474 685 993 191 1121
Fragments arranged by size:
1121 993 685 474 283 222 191 188
SfoI GGC'GCC
Cuts at: 816 1589 1837 816
Size: 773 248 3136
Fragments arranged by size:
3136 773 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4157
SmaI CCC'GGG
Cuts at: 779 1179 779
Size: 400 3757
SmlI C'TyrA_G
Cuts at: 1312 2227 2719 2981 1312
Size: 915 492 262 2488
Fragments arranged by size:
2488 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4157
SphI G_CATG'C
Cuts at: 1210 1710 1210
Size: 500 3657
StyI C'CwwG_G
Cuts at: 1291 1645 1291
Size: 354 3803
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 946 1740 1873 2332 2541 946
Size: 794 133 459 209 2562
Fragments arranged by size:
2562 794 459 209 133
TatI w'GTAC_w
Cuts at: 3672 3672
Size: 4157
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3674 3706 3674
Size: 32 4125
Tth111I GACn'n_nGTC
Cuts at: 1954 1954
Size: 4157
XbaI T'CTAG_A
Cuts at: 1188 1188
Size: 4157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1652 1652
Size: 4157
XmaI C'CCGG_G
Cuts at: 777 1177 777
Size: 400 3757
XmnI GAAnn'nnTTC
Cuts at: 901 901
Size: 4157
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI