Restriction map for
pF1KB9717
(Circular) (Six-base) MAPSORT of: pF1KB9717.seq Check: 9442 from: 1 to: 4298
pF1KB9717 4298 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1336 1336
Size: 4298
Acc65I G'GTAC_C
Cuts at: 1314 1314
Size: 4298
AclI AA'CG_TT
Cuts at: 4138 4138
Size: 4298
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1268 2123 2555 3302 3570 1268
Size: 855 432 747 268 1996
Fragments arranged by size:
1996 855 747 432 268
AflIII A'CryG_T
Cuts at: 352 1173 1650 2754 3731 352
Size: 821 477 1104 977 919
Fragments arranged by size:
1104 977 919 821 477
AgeI A'CCGG_T
Cuts at: 2686 2686
Size: 4298
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 580 899 580
Size: 319 3979
AlwNI CAG_nnn'CTG
Cuts at: 1603 3170 1603
Size: 1567 2731
ApaLI G'TGCA_C
Cuts at: 3068 3068
Size: 4298
ApoI r'AATT_y
Cuts at: 1106 1302 3651 3907 1106
Size: 196 2349 256 1497
Fragments arranged by size:
2349 1497 256 196
AseI AT'TA_AT
Cuts at: 20 20
Size: 4298
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4298
AvaI C'yCGr_G
Cuts at: 1318 1318
Size: 4298
BamHI G'GATC_C
Cuts at: 1323 1323
Size: 4298
BanI G'GyrC_C
Cuts at: 1314 1728 1976 2011 1314
Size: 414 248 35 3601
Fragments arranged by size:
3601 414 248 35
BanII G_rGCy'C
Cuts at: 1312 2342 1312
Size: 1030 3268
BbeI G_GCGC'C
Cuts at: 1732 1980 1732
Size: 248 4050
BbsI GAAGACnn'nnnn_
Cuts at: 218 329 218
Size: 111 4187
BbvCI CC'TCA_GC
Cuts at: 872 872
Size: 4298
BciVI GTATCCnnnnn_n'
Cuts at: 2196 2957 4298 2196
Size: 761 1341 2196
Fragments arranged by size:
2196 1341 761
BclI T'GATC_A
Cuts at: 396 1818 396
Size: 1422 2876
BglII A'GATC_T
Cuts at: 1813 3850 1813
Size: 2037 2261
BlpI GC'TnA_GC
Cuts at: 1410 1410
Size: 4298
Bme1580I G_kGCm'C
Cuts at: 1923 2016 3072 1923
Size: 93 1056 3149
Fragments arranged by size:
3149 1056 93
BmgBI CAC'GTC
Cuts at: 353 353
Size: 4298
BmrI ACTGGGnnnn_n'
Cuts at: 1510 1591 1926 4070 1510
Size: 81 335 2144 1738
Fragments arranged by size:
2144 1738 335 81
BmtI G_CTAG'C
Cuts at: 833 833
Size: 4298
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2665 2665
Size: 4298
Bpu10I CC'TnA_GC
Cuts at: 553 872 553
Size: 319 3979
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 819 1474 2353 2845 3143 819
Size: 655 879 492 298 1974
Fragments arranged by size:
1974 879 655 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4002 4002
Size: 4298
BsaAI yAC'GTr
Cuts at: 761 1651 761
Size: 890 3408
BsaBI GATnn'nnATC
Cuts at: 6 401 1812 3849 6
Size: 395 1411 2037 455
Fragments arranged by size:
2037 1411 455 395
BsaHI Gr'CG_yC
Cuts at: 1729 1977 2679 4175 1729
Size: 248 702 1496 1852
Fragments arranged by size:
1852 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1487 2008 2686 2960 3107 3518 1487
Size: 521 678 274 147 411 2267
Fragments arranged by size:
2267 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 812 812
Size: 4298
BseYI C'CCAG_C
Cuts at: 1723 2481 3058 3554 1723
Size: 758 577 496 2467
Fragments arranged by size:
2467 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1840 1886 3094 104
Size: 1736 46 1208 1308
Fragments arranged by size:
1736 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 254 1312 2090 2280 3072 254
Size: 1058 778 190 792 1480
Fragments arranged by size:
1480 1058 792 778 190
BsmI GAATG_Cn'
Cuts at: 850 850
Size: 4298
Bsp1286I G_dGCh'C
Cuts at: 254 1312 1923 2016 2090 2280 2342 3072 254
Size: 1058 611 93 74 190 62 730 1480
Fragments arranged by size:
1480 1058 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3518 3518
Size: 4298
BspHI T'CATG_A
Cuts at: 1233 3474 1233
Size: 2241 2057
BspMI ACCTGCnnnn'nnnn_
Cuts at: 529 1348 1562 1864 2245 529
Size: 819 214 302 381 2582
Fragments arranged by size:
2582 819 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2590 2644 4128 4293 50
Size: 2540 54 1484 165 55
Fragments arranged by size:
2540 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 881 2210 881
Size: 1329 2969
BsrFI r'CCGG_y
Cuts at: 2296 2477 2686 2296
Size: 181 209 3908
Fragments arranged by size:
3908 209 181
BssSI C'ACGA_G
Cuts at: 743 2569 2927 743
Size: 1826 358 2114
Fragments arranged by size:
2114 1826 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1675 1675
Size: 4298
BstBI TT'CG_AA
Cuts at: 2659 2659
Size: 4298
BstYI r'GATC_y
Cuts at: 1 1323 1790 1813 2148 2394 3406 3492 3504
Size: 1322 467 23 335 246 1012 86 12
Cuts at: 3504 3850 1
Size: 346 449
Fragments arranged by size:
1322 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 255 525 107
Size: 148 270 3880
Fragments arranged by size:
3880 270 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 150 2248 2429 2592 150
Size: 2098 181 163 1856
Fragments arranged by size:
2098 1856 181 163
BtsI GCAGTG_nn'
Cuts at: 1541 1541
Size: 4298
DraI TTT'AAA
Cuts at: 335 362 1297 335
Size: 27 935 3336
Fragments arranged by size:
3336 935 27
DrdI GACnn_nn'nnGTC
Cuts at: 596 2004 2862 596
Size: 1408 858 2032
Fragments arranged by size:
2032 1408 858
EaeI y'GGCC_r
Cuts at: 1883 2057 2448 2475 3820 1883
Size: 174 391 27 1345 2361
Fragments arranged by size:
2361 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1883 1883
Size: 4298
EarI CTCTTCn'nnn_
Cuts at: 2321 2531 2321
Size: 210 4088
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2506 2816 2962 4108 2506
Size: 310 146 1146 2696
Fragments arranged by size:
2696 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1310 1310
Size: 4298
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1268 2123 2555 3302 3570 1268
Size: 855 432 747 268 1996
Fragments arranged by size:
1996 855 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1437 1437
Size: 4298
EcoRI G'AATT_C
Cuts at: 1302 1302
Size: 4298
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 286 2317 286
Size: 2031 2267
FspI TGC'GCA
Cuts at: 2079 2079
Size: 4298
HaeII r_GCGC'y
Cuts at: 1732 1980 3002 3981 1732
Size: 248 1022 979 2049
Fragments arranged by size:
2049 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1263 1295 2140 2170 2172 2202 1263
Size: 32 845 30 2 30 3359
Fragments arranged by size:
3359 845 32 30 30 2
HincII GTy'rAC
Cuts at: 1337 1337
Size: 4298
KasI G'GCGC_C
Cuts at: 1728 1976 1728
Size: 248 4050
KpnI G_GTAC'C
Cuts at: 1318 1318
Size: 4298
MluI A'CGCG_T
Cuts at: 3731 3731
Size: 4298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 350 2969 3153 350
Size: 2619 184 1495
Fragments arranged by size:
2619 1495 184
MscI TGG'CCA
Cuts at: 2059 2059
Size: 4298
MslI CAynn'nnrTG
Cuts at: 290 562 935 1178 2287 2414 290
Size: 272 373 243 1109 127 2174
Fragments arranged by size:
2174 1109 373 272 243 127
MspA1I CmG'CkG
Cuts at: 1409 1723 2083 3096 3341 1409
Size: 314 360 1013 245 2366
Fragments arranged by size:
2366 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2479 2479
Size: 4298
NarI GG'CG_CC
Cuts at: 1729 1977 1729
Size: 248 4050
NcoI C'CATG_G
Cuts at: 107 255 525 107
Size: 148 270 3880
Fragments arranged by size:
3880 270 148
NgoMIV G'CCGG_C
Cuts at: 2477 2477
Size: 4298
NheI G'CTAG_C
Cuts at: 829 829
Size: 4298
NspI r_CATG'y
Cuts at: 585 1177 1351 1851 2758 585
Size: 592 174 500 907 2125
Fragments arranged by size:
2125 907 592 500 174
PciI A'CATG_T
Cuts at: 1173 2754 1173
Size: 1581 2717
PmeI GTTT'AAAC
Cuts at: 1297 1297
Size: 4298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3437 3469 3437
Size: 32 4266
PsiI TTA'TAA
Cuts at: 545 545
Size: 4298
PstI C_TGCA'G
Cuts at: 717 1345 2030 717
Size: 628 685 2985
Fragments arranged by size:
2985 685 628
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4298
PvuII CAG'CTG
Cuts at: 1723 2083 1723
Size: 360 3938
RsrII CG'GwC_CG
Cuts at: 2493 2493
Size: 4298
SacI G_AGCT'C
Cuts at: 1312 1312
Size: 4298
SalI G'TCGA_C
Cuts at: 1335 1335
Size: 4298
SapI GCTCTTCn'nnn_
Cuts at: 2321 2531 2321
Size: 210 4088
SbfI CC_TGCA'GG
Cuts at: 1345 1345
Size: 4298
ScaI AGT'ACT
Cuts at: 608 1000 3815 608
Size: 392 2815 1091
Fragments arranged by size:
2815 1091 392
SfcI C'TryA_G
Cuts at: 33 713 1341 2026 3019 3210 33
Size: 680 628 685 993 191 1121
Fragments arranged by size:
1121 993 685 680 628 191
SfoI GGC'GCC
Cuts at: 1730 1978 1730
Size: 248 4050
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4298
SmaI CCC'GGG
Cuts at: 1320 1320
Size: 4298
SmlI C'TyrA_G
Cuts at: 834 1453 2368 2860 3122 834
Size: 619 915 492 262 2010
Fragments arranged by size:
2010 915 619 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4298
SphI G_CATG'C
Cuts at: 1351 1851 1351
Size: 500 3798
SspI AAT'ATT
Cuts at: 391 651 391
Size: 260 4038
StyI C'CwwG_G
Cuts at: 107 255 525 1432 1786 107
Size: 148 270 907 354 2619
Fragments arranged by size:
2619 907 354 270 148
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1881 2014 2473 2682 1881
Size: 133 459 209 3497
Fragments arranged by size:
3497 459 209 133
TatI w'GTAC_w
Cuts at: 355 606 735 998 3813 355
Size: 251 129 263 2815 840
Fragments arranged by size:
2815 840 263 251 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3815 3847 3815
Size: 32 4266
Tth111I GACn'n_nGTC
Cuts at: 2095 2095
Size: 4298
XbaI T'CTAG_A
Cuts at: 1329 1329
Size: 4298
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1793 1793
Size: 4298
XmaI C'CCGG_G
Cuts at: 1318 1318
Size: 4298
XmnI GAAnn'nnTTC
Cuts at: 215 215
Size: 4298
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PciI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BpmI BsaXI
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI