Restriction map for
pF1KB9723
(Circular) (Six-base) MAPSORT of: pF1KB9723.seq Check: 3004 from: 1 to: 4355
pF1KB9723 4355 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 528 528
Size: 4355
AccI GT'mk_AC
Cuts at: 1393 1393
Size: 4355
Acc65I G'GTAC_C
Cuts at: 1371 1371
Size: 4355
AclI AA'CG_TT
Cuts at: 4195 4195
Size: 4355
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 2180 2612 3359 3627 571
Size: 1609 432 747 268 1299
Fragments arranged by size:
1609 1299 747 432 268
AflIII A'CryG_T
Cuts at: 776 989 1707 2811 3788 776
Size: 213 718 1104 977 1343
Fragments arranged by size:
1343 1104 977 718 213
AgeI A'CCGG_T
Cuts at: 2743 2743
Size: 4355
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1146 1146
Size: 4355
AlwNI CAG_nnn'CTG
Cuts at: 732 1206 1660 3227 732
Size: 474 454 1567 1860
Fragments arranged by size:
1860 1567 474 454
ApaI G_GGCC'C
Cuts at: 193 613 1096 193
Size: 420 483 3452
Fragments arranged by size:
3452 483 420
ApaLI G'TGCA_C
Cuts at: 3125 3125
Size: 4355
ApoI r'AATT_y
Cuts at: 1192 1359 3708 3964 1192
Size: 167 2349 256 1583
Fragments arranged by size:
2349 1583 256 167
AscI GG'CGCG_CC
Cuts at: 737 737
Size: 4355
AseI AT'TA_AT
Cuts at: 20 20
Size: 4355
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4355
AvaI C'yCGr_G
Cuts at: 613 658 832 1015 1089 1171 1375 613
Size: 45 174 183 74 82 204 3593
Fragments arranged by size:
3593 204 183 174 82 74 45
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 120 153 120
Size: 33 4322
BamHI G'GATC_C
Cuts at: 1380 1380
Size: 4355
BanI G'GyrC_C
Cuts at: 249 853 969 1371 1785 2033 2068 249
Size: 604 116 402 414 248 35 2536
Fragments arranged by size:
2536 604 414 402 248 116 35
BanII G_rGCy'C
Cuts at: 193 220 613 941 1012 1096 1169 1369 2399
Size: 27 393 328 71 84 73 200 1030
Cuts at: 2399 193
Size: 2149
Fragments arranged by size:
2149 1030 393 328 200 84 73 71
27
BbeI G_GCGC'C
Cuts at: 253 973 1789 2037 253
Size: 720 816 248 2571
Fragments arranged by size:
2571 816 720 248
BciVI GTATCCnnnnn_n'
Cuts at: 121 319 2253 3014 4355 121
Size: 198 1934 761 1341 121
Fragments arranged by size:
1934 1341 761 198 121
BclI T'GATC_A
Cuts at: 1875 1875
Size: 4355
BglI GCCn_nnn'nGGC
Cuts at: 528 617 1139 528
Size: 89 522 3744
Fragments arranged by size:
3744 522 89
BglII A'GATC_T
Cuts at: 1870 3907 1870
Size: 2037 2318
BlpI GC'TnA_GC
Cuts at: 214 587 1467 214
Size: 373 880 3102
Fragments arranged by size:
3102 880 373
Bme1580I G_kGCm'C
Cuts at: 193 613 1096 1980 2073 3129 193
Size: 420 483 884 93 1056 1419
Fragments arranged by size:
1419 1056 884 483 420 93
BmgBI CAC'GTC
Cuts at: 779 1347 779
Size: 568 3787
BmrI ACTGGGnnnn_n'
Cuts at: 1145 1567 1648 1983 4127 1145
Size: 422 81 335 2144 1373
Fragments arranged by size:
2144 1373 422 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2722 2722
Size: 4355
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 634
Size: 4355
Bpu10I CC'TnA_GC
Cuts at: 344 344
Size: 4355
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1531 2410 2902 3200 1531
Size: 879 492 298 2686
Fragments arranged by size:
2686 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 879 4059 879
Size: 3180 1175
BsaAI yAC'GTr
Cuts at: 990 1708 990
Size: 718 3637
BsaBI GATnn'nnATC
Cuts at: 6 1869 3906 6
Size: 1863 2037 455
Fragments arranged by size:
2037 1863 455
BsaHI Gr'CG_yC
Cuts at: 250 970 1310 1786 2034 2736 4232 250
Size: 720 340 476 248 702 1496 373
Fragments arranged by size:
1496 720 702 476 373 340 248
BsaWI w'CCGG_w
Cuts at: 1116 1258 1544 2065 2743 3017 3164 3575 1116
Size: 142 286 521 678 274 147 411 1896
Fragments arranged by size:
1896 678 521 411 286 274 147 142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1300 1330 1300
Size: 30 4325
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 478 1159 478
Size: 681 3674
BseYI C'CCAG_C
Cuts at: 186 411 606 976 1780 2538 3115 3611 186
Size: 225 195 370 804 758 577 496 930
Fragments arranged by size:
930 804 758 577 496 370 225 195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 505 559 1192 370
Size: 135 54 633 3533
Fragments arranged by size:
3533 633 135 54
BsiEI CG_ry'CG
Cuts at: 104 275 319 523 602 624 851 1897 1943
Size: 171 44 204 79 22 227 1046 46
Cuts at: 1943 3151 104
Size: 1208 1308
Fragments arranged by size:
1308 1208 1046 227 204 171 79 46
44 22
BsiHKAI G_wGCw'C
Cuts at: 270 1169 1369 2147 2337 3129 270
Size: 899 200 778 190 792 1496
Fragments arranged by size:
1496 899 792 778 200 190
Bsp1286I G_dGCh'C
Cuts at: 193 220 270 613 941 1012 1096 1169 1369
Size: 27 50 343 328 71 84 73 200
Cuts at: 1369 1980 2073 2147 2337 2399 3129 193
Size: 611 93 74 190 62 730 1419
Fragments arranged by size:
1419 730 611 343 328 200 190 93
84 74 73 71 62 50 27
BspEI T'CCGG_A
Cuts at: 1116 3575 1116
Size: 2459 1896
BspHI T'CATG_A
Cuts at: 3531 3531
Size: 4355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 392 528 861 1405 1619 1921 2302 392
Size: 136 333 544 214 302 381 2445
Fragments arranged by size:
2445 544 381 333 302 214 136
BsrBI CCG'CTC
Cuts at: 50 474 747 2647 2701 4185 4350 50
Size: 424 273 1900 54 1484 165 55
Fragments arranged by size:
1900 1484 424 273 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2267 2267
Size: 4355
BsrFI r'CCGG_y
Cuts at: 252 270 518 676 850 907 2353 2534 2743
Size: 18 248 158 174 57 1446 181 209
Cuts at: 2743 252
Size: 1864
Fragments arranged by size:
1864 1446 248 209 181 174 158 57
18
BssHII G'CGCG_C
Cuts at: 161 737 161
Size: 576 3779
BssSI C'ACGA_G
Cuts at: 2626 2984 2626
Size: 358 3997
BstAPI GCAn_nnn'nTGC
Cuts at: 492 1732 492
Size: 1240 3115
BstBI TT'CG_AA
Cuts at: 2716 2716
Size: 4355
BstYI r'GATC_y
Cuts at: 1 1380 1847 1870 2205 2451 3463 3549 3561
Size: 1379 467 23 335 246 1012 86 12
Cuts at: 3561 3907 1
Size: 346 449
Fragments arranged by size:
1379 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 170 281 523 632 941 1281 1319 170
Size: 111 242 109 309 340 38 3206
Fragments arranged by size:
3206 340 309 242 111 109 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1018 2305 2486 2649 1018
Size: 1287 181 163 2724
Fragments arranged by size:
2724 1287 181 163
BtsI GCAGTG_nn'
Cuts at: 1123 1598 1123
Size: 475 3880
DraI TTT'AAA
Cuts at: 1354 1354
Size: 4355
DraIII CAC_nnn'GTG
Cuts at: 1215 1215
Size: 4355
DrdI GACnn_nn'nnGTC
Cuts at: 2061 2919 2061
Size: 858 3497
EaeI y'GGCC_r
Cuts at: 179 272 316 520 599 621 629 708 848
Size: 93 44 204 79 22 8 79 140
Cuts at: 848 1940 2114 2505 2532 3877 179
Size: 1092 174 391 27 1345 657
Fragments arranged by size:
1345 1092 657 391 204 174 140 93
79 79 44 27 22 8
EagI C'GGCC_G
Cuts at: 272 316 520 599 621 848 1940 272
Size: 44 204 79 22 227 1092 2687
Fragments arranged by size:
2687 1092 227 204 79 44 22
EarI CTCTTCn'nnn_
Cuts at: 2378 2588 2378
Size: 210 4145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 457 2563 2873 3019 4165 457
Size: 2106 310 146 1146 647
Fragments arranged by size:
2106 1146 647 310 146
EcoICRI GAG'CTC
Cuts at: 1167 1367 1167
Size: 200 4155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 634 2180 2612 3359 3627 571
Size: 63 1546 432 747 268 1299
Fragments arranged by size:
1546 1299 747 432 268 63
EcoNI CCTnn'n_nnAGG
Cuts at: 1174 1174
Size: 4355
EcoO109I rG'GnC_Cy
Cuts at: 190 610 946 1093 1494 190
Size: 420 336 147 401 3051
Fragments arranged by size:
3051 420 401 336 147
EcoRI G'AATT_C
Cuts at: 1359 1359
Size: 4355
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 471 2374 471
Size: 1903 2452
FspI TGC'GCA
Cuts at: 2136 2136
Size: 4355
HaeII r_GCGC'y
Cuts at: 253 433 973 983 1789 2037 3059 4038 253
Size: 180 540 10 806 248 1022 979 570
Fragments arranged by size:
1022 979 806 570 540 248 180 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1300 1332 2197 2227 2229 2259 1300
Size: 32 865 30 2 30 3396
Fragments arranged by size:
3396 865 32 30 30 2
HincII GTy'rAC
Cuts at: 1394 1394
Size: 4355
KasI G'GCGC_C
Cuts at: 249 969 1785 2033 249
Size: 720 816 248 2571
Fragments arranged by size:
2571 816 720 248
KpnI G_GTAC'C
Cuts at: 1375 1375
Size: 4355
MluI A'CGCG_T
Cuts at: 3788 3788
Size: 4355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1331 3026 3210 1331
Size: 1695 184 2476
Fragments arranged by size:
2476 1695 184
MscI TGG'CCA
Cuts at: 631 2116 631
Size: 1485 2870
MslI CAynn'nnrTG
Cuts at: 235 2344 2471 235
Size: 2109 127 2119
Fragments arranged by size:
2119 2109 127
MspA1I CmG'CkG
Cuts at: 172 263 283 525 687 786 943 1321 1466
Size: 91 20 242 162 99 157 378 145
Cuts at: 1466 1780 2140 3153 3398 172
Size: 314 360 1013 245 1129
Fragments arranged by size:
1129 1013 378 360 314 245 242 162
157 145 99 91 20
NaeI GCC'GGC
Cuts at: 254 678 852 2536 254
Size: 424 174 1684 2073
Fragments arranged by size:
2073 1684 424 174
NarI GG'CG_CC
Cuts at: 250 970 1786 2034 250
Size: 720 816 248 2571
Fragments arranged by size:
2571 816 720 248
NcoI C'CATG_G
Cuts at: 632 632
Size: 4355
NgoMIV G'CCGG_C
Cuts at: 252 676 850 2534 252
Size: 424 174 1684 2073
Fragments arranged by size:
2073 1684 424 174
NotI GC'GGCC_GC
Cuts at: 599 621 599
Size: 22 4333
NspI r_CATG'y
Cuts at: 495 1408 1908 2815 495
Size: 913 500 907 2035
Fragments arranged by size:
2035 913 907 500
PasI CC'CwG_GG
Cuts at: 200 200
Size: 4355
PciI A'CATG_T
Cuts at: 2811 2811
Size: 4355
PflMI CCAn_nnn'nTGG
Cuts at: 732 732
Size: 4355
PmeI GTTT'AAAC
Cuts at: 1354 1354
Size: 4355
PmlI CAC'GTG
Cuts at: 990 990
Size: 4355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3494 3526 3494
Size: 32 4323
PpuMI rG'GwC_Cy
Cuts at: 946 946
Size: 4355
PshAI GACnn'nnGTC
Cuts at: 1104 1151 1104
Size: 47 4308
PspOMI G'GGCC_C
Cuts at: 189 609 1092 189
Size: 420 483 3452
Fragments arranged by size:
3452 483 420
PstI C_TGCA'G
Cuts at: 1402 2087 1402
Size: 685 3670
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4355
PvuII CAG'CTG
Cuts at: 1780 2140 1780
Size: 360 3995
RsrII CG'GwC_CG
Cuts at: 2550 2550
Size: 4355
SacI G_AGCT'C
Cuts at: 1169 1369 1169
Size: 200 4155
SacII CC_GC'GG
Cuts at: 173 284 526 944 1322 173
Size: 111 242 418 378 3206
Fragments arranged by size:
3206 418 378 242 111
SalI G'TCGA_C
Cuts at: 1392 1392
Size: 4355
SanDI GG'GwC_CC
Cuts at: 946 946
Size: 4355
SapI GCTCTTCn'nnn_
Cuts at: 2378 2588 2378
Size: 210 4145
SbfI CC_TGCA'GG
Cuts at: 1402 1402
Size: 4355
ScaI AGT'ACT
Cuts at: 3872 3872
Size: 4355
SfcI C'TryA_G
Cuts at: 33 858 1398 2083 3076 3267 33
Size: 825 540 685 993 191 1121
Fragments arranged by size:
1121 993 825 685 540 191
SfoI GGC'GCC
Cuts at: 251 971 1787 2035 251
Size: 720 816 248 2571
Fragments arranged by size:
2571 816 720 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4355
SgrAI Cr'CCGG_yG
Cuts at: 252 252
Size: 4355
SmaI CCC'GGG
Cuts at: 834 1017 1091 1377 834
Size: 183 74 286 3812
Fragments arranged by size:
3812 286 183 74
SmlI C'TyrA_G
Cuts at: 1510 2425 2917 3179 1510
Size: 915 492 262 2686
Fragments arranged by size:
2686 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4355
SphI G_CATG'C
Cuts at: 1408 1908 1408
Size: 500 3855
StyI C'CwwG_G
Cuts at: 293 632 1489 1843 293
Size: 339 857 354 2805
Fragments arranged by size:
2805 857 354 339
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 990 1938 2071 2530 2739 990
Size: 948 133 459 209 2606
Fragments arranged by size:
2606 948 459 209 133
TatI w'GTAC_w
Cuts at: 992 3870 992
Size: 2878 1477
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3872 3904 3872
Size: 32 4323
Tth111I GACn'n_nGTC
Cuts at: 2152 2152
Size: 4355
XbaI T'CTAG_A
Cuts at: 1386 1386
Size: 4355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 639 1296 1850 639
Size: 657 554 3144
Fragments arranged by size:
3144 657 554
XmaI C'CCGG_G
Cuts at: 832 1015 1089 1375 832
Size: 183 74 286 3812
Fragments arranged by size:
3812 286 183 74
XmnI GAAnn'nnTTC
Cuts at: 328 328
Size: 4355
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AvrII BbsI
BbvCI BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NruI NsiI
PacI PfoI PsiI PspXI PsrI SexAI SfiI SnaBI
SrfI SspI StuI SwaI XhoI ZraI