Restriction map for
pF1KB9726
(Circular) (Six-base) MAPSORT of: pF1KB9726.seq Check: 6146 from: 1 to: 4406
pF1KB9726 4406 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1444 1444
Size: 4406
Acc65I G'GTAC_C
Cuts at: 1422 1422
Size: 4406
AclI AA'CG_TT
Cuts at: 4246 4246
Size: 4406
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2231 2663 3410 3678 2231
Size: 432 747 268 2959
Fragments arranged by size:
2959 747 432 268
AfeI AGC'GCT
Cuts at: 253 253
Size: 4406
AflIII A'CryG_T
Cuts at: 1758 2862 3839 1758
Size: 1104 977 2325
Fragments arranged by size:
2325 1104 977
AgeI A'CCGG_T
Cuts at: 2794 2794
Size: 4406
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 709 741 709
Size: 32 4374
AlwNI CAG_nnn'CTG
Cuts at: 276 1711 3278 276
Size: 1435 1567 1404
Fragments arranged by size:
1567 1435 1404
ApaI G_GGCC'C
Cuts at: 434 883 434
Size: 449 3957
ApaLI G'TGCA_C
Cuts at: 3176 3176
Size: 4406
ApoI r'AATT_y
Cuts at: 1410 3759 4015 1410
Size: 2349 256 1801
Fragments arranged by size:
2349 1801 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4406
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
AvaI C'yCGr_G
Cuts at: 346 654 1087 1426 346
Size: 308 433 339 3326
Fragments arranged by size:
3326 433 339 308
BamHI G'GATC_C
Cuts at: 1185 1431 1185
Size: 246 4160
BanI G'GyrC_C
Cuts at: 466 1054 1083 1123 1272 1284 1422 1836 2084
Size: 588 29 40 149 12 138 414 248
Cuts at: 2084 2119 466
Size: 35 2753
Fragments arranged by size:
2753 588 414 248 149 138 40 35
29 12
BanII G_rGCy'C
Cuts at: 314 353 434 652 883 973 1420 2450 314
Size: 39 81 218 231 90 447 1030 2270
Fragments arranged by size:
2270 1030 447 231 218 90 81 39
BbeI G_GCGC'C
Cuts at: 1058 1087 1127 1276 1288 1840 2088 1058
Size: 29 40 149 12 552 248 3376
Fragments arranged by size:
3376 552 248 149 40 29 12
BbvCI CC'TCA_GC
Cuts at: 787 787
Size: 4406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 168 202 90
Size: 34 44 34 4294
Fragments arranged by size:
4294 44 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2304 3065 4406 2304
Size: 761 1341 2304
Fragments arranged by size:
2304 1341 761
BclI T'GATC_A
Cuts at: 1926 1926
Size: 4406
BglII A'GATC_T
Cuts at: 806 1921 3958 806
Size: 1115 2037 1254
Fragments arranged by size:
2037 1254 1115
BlpI GC'TnA_GC
Cuts at: 255 1518 255
Size: 1263 3143
Bme1580I G_kGCm'C
Cuts at: 434 883 2031 2124 3180 434
Size: 449 1148 93 1056 1660
Fragments arranged by size:
1660 1148 1056 449 93
BmgBI CAC'GTC
Cuts at: 1104 1104
Size: 4406
BmrI ACTGGGnnnn_n'
Cuts at: 377 1618 1699 2034 4178 377
Size: 1241 81 335 2144 605
Fragments arranged by size:
2144 1241 605 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2773 2773
Size: 4406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 736 872 256
Size: 480 136 3790
Fragments arranged by size:
3790 480 136
Bpu10I CC'TnA_GC
Cuts at: 787 787
Size: 4406
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1582 2461 2953 3251 1582
Size: 879 492 298 2737
Fragments arranged by size:
2737 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4110 4110
Size: 4406
BsaAI yAC'GTr
Cuts at: 1164 1759 1164
Size: 595 3811
BsaBI GATnn'nnATC
Cuts at: 6 805 1920 3957 6
Size: 799 1115 2037 455
Fragments arranged by size:
2037 1115 799 455
BsaHI Gr'CG_yC
Cuts at: 667 1055 1084 1124 1135 1273 1285 1837 2085
Size: 388 29 40 11 138 12 552 248
Cuts at: 2085 2787 4283 667
Size: 702 1496 790
Fragments arranged by size:
1496 790 702 552 388 248 138 40
29 12 11
BsaWI w'CCGG_w
Cuts at: 1155 1595 2116 2794 3068 3215 3626 1155
Size: 440 521 678 274 147 411 1935
Fragments arranged by size:
1935 678 521 440 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 709 739 709
Size: 30 4376
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1329 1346 1349 1329
Size: 17 3 4386
Fragments arranged by size:
4386 17 3
BseYI C'CCAG_C
Cuts at: 433 958 1288 1831 2589 3166 3662 433
Size: 525 330 543 758 577 496 1177
Fragments arranged by size:
1177 758 577 543 525 496 330
BsiEI CG_ry'CG
Cuts at: 104 926 1948 1994 3202 104
Size: 822 1022 46 1208 1308
Fragments arranged by size:
1308 1208 1022 822 46
BsiHKAI G_wGCw'C
Cuts at: 314 1420 2198 2388 3180 314
Size: 1106 778 190 792 1540
Fragments arranged by size:
1540 1106 792 778 190
BsmI GAATG_Cn'
Cuts at: 1011 1011
Size: 4406
Bsp1286I G_dGCh'C
Cuts at: 314 353 434 652 883 973 1420 2031 2124
Size: 39 81 218 231 90 447 611 93
Cuts at: 2124 2198 2388 2450 3180 314
Size: 74 190 62 730 1540
Fragments arranged by size:
1540 730 611 447 231 218 190 93
90 81 74 62 39
BspEI T'CCGG_A
Cuts at: 3626 3626
Size: 4406
BspHI T'CATG_A
Cuts at: 932 3582 932
Size: 2650 1756
BspMI ACCTGCnnnn'nnnn_
Cuts at: 785 1456 1670 1972 2353 785
Size: 671 214 302 381 2838
Fragments arranged by size:
2838 671 381 302 214
BsrBI CCG'CTC
Cuts at: 50 795 2698 2752 4236 4401 50
Size: 745 1903 54 1484 165 55
Fragments arranged by size:
1903 1484 745 165 55 54
BsrDI GCAATG_nn'
Cuts at: 186 2318 186
Size: 2132 2274
BsrFI r'CCGG_y
Cuts at: 749 821 892 925 1204 2404 2585 2794 749
Size: 72 71 33 279 1200 181 209 2361
Fragments arranged by size:
2361 1200 279 209 181 72 71 33
BssHII G'CGCG_C
Cuts at: 698 698
Size: 4406
BssSI C'ACGA_G
Cuts at: 693 2677 3035 693
Size: 1984 358 2064
Fragments arranged by size:
2064 1984 358
BstAPI GCAn_nnn'nTGC
Cuts at: 912 1783 912
Size: 871 3535
BstBI TT'CG_AA
Cuts at: 2767 2767
Size: 4406
BstXI CCAn_nnnn'nTGG
Cuts at: 234 861 234
Size: 627 3779
BstYI r'GATC_y
Cuts at: 1 806 1185 1431 1898 1921 2256 2502 3514
Size: 805 379 246 467 23 335 246 1012
Cuts at: 3514 3600 3612 3958 1
Size: 86 12 346 449
Fragments arranged by size:
1012 805 467 449 379 346 335 246
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1361 1361
Size: 4406
BtgI C'CryG_G
Cuts at: 137 920 1299 137
Size: 783 379 3244
Fragments arranged by size:
3244 783 379
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2356 2537 2700 2356
Size: 181 163 4062
Fragments arranged by size:
4062 181 163
BtsI GCAGTG_nn'
Cuts at: 606 1649 606
Size: 1043 3363
DraI TTT'AAA
Cuts at: 1405 1405
Size: 4406
DrdI GACnn_nn'nnGTC
Cuts at: 2112 2970 2112
Size: 858 3548
EaeI y'GGCC_r
Cuts at: 770 923 1991 2165 2556 2583 3928 770
Size: 153 1068 174 391 27 1345 1248
Fragments arranged by size:
1345 1248 1068 391 174 153 27
EagI C'GGCC_G
Cuts at: 923 1991 923
Size: 1068 3338
EarI CTCTTCn'nnn_
Cuts at: 154 2429 2639 154
Size: 2275 210 1921
Fragments arranged by size:
2275 1921 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 608 2614 2924 3070 4216 608
Size: 2006 310 146 1146 798
Fragments arranged by size:
2006 1146 798 310 146
EcoICRI GAG'CTC
Cuts at: 312 1418 312
Size: 1106 3300
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 736 872 2231 2663 3410 3678 256
Size: 480 136 1359 432 747 268 984
Fragments arranged by size:
1359 984 747 480 432 268 136
EcoNI CCTnn'n_nnAGG
Cuts at: 1043 1043
Size: 4406
EcoO109I rG'GnC_Cy
Cuts at: 879 1545 879
Size: 666 3740
EcoRI G'AATT_C
Cuts at: 1410 1410
Size: 4406
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 4406
FspI TGC'GCA
Cuts at: 2187 2187
Size: 4406
HaeII r_GCGC'y
Cuts at: 255 1058 1087 1127 1276 1288 1384 1840 2088
Size: 803 29 40 149 12 96 456 248
Cuts at: 2088 3110 4089 255
Size: 1022 979 572
Fragments arranged by size:
1022 979 803 572 456 248 149 96
40 29 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 321 340 353 372 958 990 1324 1356 2248
Size: 19 13 19 586 32 334 32 892
Cuts at: 2248 2278 2280 2310 321
Size: 30 2 30 2417
Fragments arranged by size:
2417 892 586 334 32 32 30 30
19 19 13 2
HincII GTy'rAC
Cuts at: 1445 1445
Size: 4406
KasI G'GCGC_C
Cuts at: 1054 1083 1123 1272 1284 1836 2084 1054
Size: 29 40 149 12 552 248 3376
Fragments arranged by size:
3376 552 248 149 40 29 12
KpnI G_GTAC'C
Cuts at: 1426 1426
Size: 4406
MluI A'CGCG_T
Cuts at: 3839 3839
Size: 4406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1049 1099 3077 3261 1049
Size: 50 1978 184 2194
Fragments arranged by size:
2194 1978 184 50
MscI TGG'CCA
Cuts at: 772 2167 772
Size: 1395 3011
MslI CAynn'nnrTG
Cuts at: 1004 2395 2522 1004
Size: 1391 127 2888
Fragments arranged by size:
2888 1391 127
MspA1I CmG'CkG
Cuts at: 139 323 611 705 1191 1517 1831 2191 3204
Size: 184 288 94 486 326 314 360 1013
Cuts at: 3204 3449 139
Size: 245 1096
Fragments arranged by size:
1096 1013 486 360 326 314 288 245
184 94
NaeI GCC'GGC
Cuts at: 751 894 927 1206 2587 751
Size: 143 33 279 1381 2570
Fragments arranged by size:
2570 1381 279 143 33
NarI GG'CG_CC
Cuts at: 1055 1084 1124 1273 1285 1837 2085 1055
Size: 29 40 149 12 552 248 3376
Fragments arranged by size:
3376 552 248 149 40 29 12
NgoMIV G'CCGG_C
Cuts at: 749 892 925 1204 2585 749
Size: 143 33 279 1381 2570
Fragments arranged by size:
2570 1381 279 143 33
NspI r_CATG'y
Cuts at: 298 1459 1959 2866 298
Size: 1161 500 907 1838
Fragments arranged by size:
1838 1161 907 500
PasI CC'CwG_GG
Cuts at: 276 276
Size: 4406
PciI A'CATG_T
Cuts at: 2862 2862
Size: 4406
PfoI T'CCnGG_A
Cuts at: 1249 1249
Size: 4406
PmeI GTTT'AAAC
Cuts at: 1405 1405
Size: 4406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 709 741 3545 3577 709
Size: 32 2804 32 1538
Fragments arranged by size:
2804 1538 32 32
PspOMI G'GGCC_C
Cuts at: 430 879 430
Size: 449 3957
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1246 1278 1246
Size: 32 4374
PstI C_TGCA'G
Cuts at: 272 1453 2138 272
Size: 1181 685 2540
Fragments arranged by size:
2540 1181 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4406
PvuII CAG'CTG
Cuts at: 323 705 1831 2191 323
Size: 382 1126 360 2538
Fragments arranged by size:
2538 1126 382 360
RsrII CG'GwC_CG
Cuts at: 2601 2601
Size: 4406
SacI G_AGCT'C
Cuts at: 314 1420 314
Size: 1106 3300
SacII CC_GC'GG
Cuts at: 140 140
Size: 4406
SalI G'TCGA_C
Cuts at: 1443 1443
Size: 4406
SapI GCTCTTCn'nnn_
Cuts at: 2429 2639 2429
Size: 210 4196
SbfI CC_TGCA'GG
Cuts at: 1453 1453
Size: 4406
ScaI AGT'ACT
Cuts at: 3923 3923
Size: 4406
SexAI A'CCwGG_T
Cuts at: 1397 1397
Size: 4406
SfcI C'TryA_G
Cuts at: 33 268 1449 2134 3127 3318 33
Size: 235 1181 685 993 191 1121
Fragments arranged by size:
1181 1121 993 685 235 191
SfoI GGC'GCC
Cuts at: 1056 1085 1125 1274 1286 1838 2086 1056
Size: 29 40 149 12 552 248 3376
Fragments arranged by size:
3376 552 248 149 40 29 12
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
SmaI CCC'GGG
Cuts at: 656 1428 656
Size: 772 3634
SmlI C'TyrA_G
Cuts at: 1561 2476 2968 3230 1561
Size: 915 492 262 2737
Fragments arranged by size:
2737 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4406
SphI G_CATG'C
Cuts at: 1459 1959 1459
Size: 500 3906
StyI C'CwwG_G
Cuts at: 854 1540 1894 854
Size: 686 354 3366
Fragments arranged by size:
3366 686 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1989 2122 2581 2790 1989
Size: 133 459 209 3605
Fragments arranged by size:
3605 459 209 133
TatI w'GTAC_w
Cuts at: 833 3921 833
Size: 3088 1318
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3923 3955 3923
Size: 32 4374
Tth111I GACn'n_nGTC
Cuts at: 2203 2203
Size: 4406
XbaI T'CTAG_A
Cuts at: 1437 1437
Size: 4406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1901 1901
Size: 4406
XmaI C'CCGG_G
Cuts at: 654 1426 654
Size: 772 3634
XmnI GAAnn'nnTTC
Cuts at: 724 724
Size: 4406
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AscI AvrII
BaeI BbsI BfrBI BglI BmtI BsgI BsiWI BsmBI
BsrGI BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PflMI PmlI PpuMI PshAI PsiI
PspXI SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI