Restriction map for
pF1KB9731
(Circular) (Six-base) MAPSORT of: pF1KB9731.seq Check: 1710 from: 1 to: 4535
pF1KB9731 4535 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 192 797 1044 192
Size: 605 247 3683
Fragments arranged by size:
3683 605 247
AccI GT'mk_AC
Cuts at: 131 1573 131
Size: 1442 3093
Acc65I G'GTAC_C
Cuts at: 1551 1551
Size: 4535
AclI AA'CG_TT
Cuts at: 4375 4375
Size: 4535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2360 2792 3539 3807 2360
Size: 432 747 268 3088
Fragments arranged by size:
3088 747 432 268
AflIII A'CryG_T
Cuts at: 224 383 1887 2991 3968 224
Size: 159 1504 1104 977 791
Fragments arranged by size:
1504 1104 977 791 159
AgeI A'CCGG_T
Cuts at: 2923 2923
Size: 4535
AleI CACnn'nnGTG
Cuts at: 323 323
Size: 4535
AlwNI CAG_nnn'CTG
Cuts at: 693 942 1435 1840 3407 693
Size: 249 493 405 1567 1821
Fragments arranged by size:
1821 1567 493 405 249
ApaLI G'TGCA_C
Cuts at: 3305 3305
Size: 4535
ApoI r'AATT_y
Cuts at: 187 1539 3888 4144 187
Size: 1352 2349 256 578
Fragments arranged by size:
2349 1352 578 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4535
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
AvaI C'yCGr_G
Cuts at: 330 1555 330
Size: 1225 3310
AvrII C'CTAG_G
Cuts at: 840 840
Size: 4535
BamHI G'GATC_C
Cuts at: 1560 1560
Size: 4535
BanI G'GyrC_C
Cuts at: 241 325 1551 1965 2213 2248 241
Size: 84 1226 414 248 35 2528
Fragments arranged by size:
2528 1226 414 248 84 35
BanII G_rGCy'C
Cuts at: 740 774 1442 1549 2579 740
Size: 34 668 107 1030 2696
Fragments arranged by size:
2696 1030 668 107 34
BbeI G_GCGC'C
Cuts at: 1969 2217 1969
Size: 248 4287
BbvCI CC'TCA_GC
Cuts at: 1342 1342
Size: 4535
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 136 170 90
Size: 34 12 34 4455
Fragments arranged by size:
4455 34 34 12
BciVI GTATCCnnnnn_n'
Cuts at: 2433 3194 4535 2433
Size: 761 1341 2433
Fragments arranged by size:
2433 1341 761
BclI T'GATC_A
Cuts at: 2055 2055
Size: 4535
BglII A'GATC_T
Cuts at: 2050 4087 2050
Size: 2037 2498
BlpI GC'TnA_GC
Cuts at: 1012 1647 1012
Size: 635 3900
Bme1580I G_kGCm'C
Cuts at: 2160 2253 3309 2160
Size: 93 1056 3386
Fragments arranged by size:
3386 1056 93
BmgBI CAC'GTC
Cuts at: 227 831 227
Size: 604 3931
BmrI ACTGGGnnnn_n'
Cuts at: 1747 1828 2163 4307 1747
Size: 81 335 2144 1975
Fragments arranged by size:
2144 1975 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2902 2902
Size: 4535
Bpu10I CC'TnA_GC
Cuts at: 1342 1428 1342
Size: 86 4449
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 1711 2590 3082 3380 442
Size: 1269 879 492 298 1597
Fragments arranged by size:
1597 1269 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1393 4239 1393
Size: 2846 1689
BsaAI yAC'GTr
Cuts at: 1888 1888
Size: 4535
BsaBI GATnn'nnATC
Cuts at: 6 2049 4086 6
Size: 2043 2037 455
Fragments arranged by size:
2043 2037 455
BsaHI Gr'CG_yC
Cuts at: 1966 2214 2916 4412 1966
Size: 248 702 1496 2089
Fragments arranged by size:
2089 1496 702 248
BsaWI w'CCGG_w
Cuts at: 359 512 1724 2245 2923 3197 3344 3755 359
Size: 153 1212 521 678 274 147 411 1139
Fragments arranged by size:
1212 1139 678 521 411 274 153 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 373 403 574 604 373
Size: 30 171 30 4304
Fragments arranged by size:
4304 171 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 378 378
Size: 4535
BseYI C'CCAG_C
Cuts at: 168 723 1230 1243 1499 1960 2718 3295 3791
Size: 555 507 13 256 461 758 577 496
Cuts at: 3791 168
Size: 912
Fragments arranged by size:
912 758 577 555 507 496 461 256
13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 143 220 1072 1258 143
Size: 77 852 186 3420
Fragments arranged by size:
3420 852 186 77
BsiEI CG_ry'CG
Cuts at: 104 1083 2077 2123 3331 104
Size: 979 994 46 1208 1308
Fragments arranged by size:
1308 1208 994 979 46
BsiHKAI G_wGCw'C
Cuts at: 740 788 1549 2327 2517 3309 740
Size: 48 761 778 190 792 1966
Fragments arranged by size:
1966 792 778 761 190 48
BsmBI CGTCTCn'nnnn_
Cuts at: 618 618
Size: 4535
Bsp1286I G_dGCh'C
Cuts at: 740 774 788 1442 1549 2160 2253 2327 2517
Size: 34 14 654 107 611 93 74 190
Cuts at: 2517 2579 3309 740
Size: 62 730 1966
Fragments arranged by size:
1966 730 654 611 190 107 93 74
62 34 14
BspEI T'CCGG_A
Cuts at: 359 3755 359
Size: 3396 1139
BspHI T'CATG_A
Cuts at: 3711 3711
Size: 4535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 192 797 1044 1585 1799 2101 2482 192
Size: 605 247 541 214 302 381 2245
Fragments arranged by size:
2245 605 541 381 302 247 214
BsrBI CCG'CTC
Cuts at: 50 507 2827 2881 4365 4530 50
Size: 457 2320 54 1484 165 55
Fragments arranged by size:
2320 1484 457 165 55 54
BsrDI GCAATG_nn'
Cuts at: 225 852 2447 225
Size: 627 1595 2313
Fragments arranged by size:
2313 1595 627
BsrFI r'CCGG_y
Cuts at: 404 2533 2714 2923 404
Size: 2129 181 209 2016
Fragments arranged by size:
2129 2016 209 181
BssSI C'ACGA_G
Cuts at: 2806 3164 2806
Size: 358 4177
BstAPI GCAn_nnn'nTGC
Cuts at: 792 1224 1258 1912 792
Size: 432 34 654 3415
Fragments arranged by size:
3415 654 432 34
BstBI TT'CG_AA
Cuts at: 2896 2896
Size: 4535
BstXI CCAn_nnnn'nTGG
Cuts at: 267 267
Size: 4535
BstYI r'GATC_y
Cuts at: 1 811 1169 1560 2027 2050 2385 2631 3643
Size: 810 358 391 467 23 335 246 1012
Cuts at: 3643 3729 3741 4087 1
Size: 86 12 346 449
Fragments arranged by size:
1012 810 467 449 391 358 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 308 815 308
Size: 507 4028
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 642 1262 2485 2666 2829 642
Size: 620 1223 181 163 2348
Fragments arranged by size:
2348 1223 620 181 163
BtsI GCAGTG_nn'
Cuts at: 415 867 1778 415
Size: 452 911 3172
Fragments arranged by size:
3172 911 452
ClaI AT'CG_AT
Cuts at: 950 950
Size: 4535
DraI TTT'AAA
Cuts at: 1534 1534
Size: 4535
DrdI GACnn_nn'nnGTC
Cuts at: 856 2241 3099 856
Size: 1385 858 2292
Fragments arranged by size:
2292 1385 858
EaeI y'GGCC_r
Cuts at: 143 1101 1196 2120 2294 2685 2712 4057 143
Size: 958 95 924 174 391 27 1345 621
Fragments arranged by size:
1345 958 924 621 391 174 95 27
EagI C'GGCC_G
Cuts at: 2120 2120
Size: 4535
EarI CTCTTCn'nnn_
Cuts at: 1192 2558 2768 1192
Size: 1366 210 2959
Fragments arranged by size:
2959 1366 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 595 898 2743 3053 3199 4345 595
Size: 303 1845 310 146 1146 785
Fragments arranged by size:
1845 1146 785 310 303 146
EcoICRI GAG'CTC
Cuts at: 738 1547 738
Size: 809 3726
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2360 2792 3539 3807 2360
Size: 432 747 268 3088
Fragments arranged by size:
3088 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1216 1288 1216
Size: 72 4463
EcoO109I rG'GnC_Cy
Cuts at: 585 1238 1674 585
Size: 653 436 3446
Fragments arranged by size:
3446 653 436
EcoRI G'AATT_C
Cuts at: 1539 1539
Size: 4535
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 372 483 2554 372
Size: 111 2071 2353
Fragments arranged by size:
2353 2071 111
FspI TGC'GCA
Cuts at: 2316 2316
Size: 4535
HaeII r_GCGC'y
Cuts at: 281 1969 2217 3239 4218 281
Size: 1688 248 1022 979 598
Fragments arranged by size:
1688 1022 979 598 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 507 539 574 606 2377 2407 2409 2439 507
Size: 32 35 32 1771 30 2 30 2603
Fragments arranged by size:
2603 1771 35 32 32 30 30 2
HincII GTy'rAC
Cuts at: 132 209 1574 132
Size: 77 1365 3093
Fragments arranged by size:
3093 1365 77
KasI G'GCGC_C
Cuts at: 1965 2213 1965
Size: 248 4287
KpnI G_GTAC'C
Cuts at: 1555 1555
Size: 4535
MluI A'CGCG_T
Cuts at: 3968 3968
Size: 4535
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3206 3390 3206
Size: 184 4351
MscI TGG'CCA
Cuts at: 1198 2296 1198
Size: 1098 3437
MslI CAynn'nnrTG
Cuts at: 139 323 2524 2651 139
Size: 184 2201 127 2023
Fragments arranged by size:
2201 2023 184 127
MspA1I CmG'CkG
Cuts at: 690 1646 1960 2320 3333 3578 690
Size: 956 314 360 1013 245 1647
Fragments arranged by size:
1647 1013 956 360 314 245
NaeI GCC'GGC
Cuts at: 406 2716 406
Size: 2310 2225
NarI GG'CG_CC
Cuts at: 1966 2214 1966
Size: 248 4287
NcoI C'CATG_G
Cuts at: 815 815
Size: 4535
NdeI CA'TA_TG
Cuts at: 1298 1298
Size: 4535
NgoMIV G'CCGG_C
Cuts at: 404 2714 404
Size: 2310 2225
NspI r_CATG'y
Cuts at: 387 1588 2088 2995 387
Size: 1201 500 907 1927
Fragments arranged by size:
1927 1201 907 500
PasI CC'CwG_GG
Cuts at: 1376 1376
Size: 4535
PciI A'CATG_T
Cuts at: 383 2991 383
Size: 2608 1927
PfoI T'CCnGG_A
Cuts at: 616 935 616
Size: 319 4216
PmeI GTTT'AAAC
Cuts at: 1534 1534
Size: 4535
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 3674
Size: 32 4503
PpuMI rG'GwC_Cy
Cuts at: 585 1238 585
Size: 653 3882
PspXI vC'TCGA_Gb
Cuts at: 330 330
Size: 4535
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 370 402 370
Size: 32 4503
PstI C_TGCA'G
Cuts at: 397 454 578 947 1582 2267 397
Size: 57 124 369 635 685 2665
Fragments arranged by size:
2665 685 635 369 124 57
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4535
PvuII CAG'CTG
Cuts at: 690 1960 2320 690
Size: 1270 360 2905
Fragments arranged by size:
2905 1270 360
RsrII CG'GwC_CG
Cuts at: 2730 2730
Size: 4535
SacI G_AGCT'C
Cuts at: 740 1549 740
Size: 809 3726
SalI G'TCGA_C
Cuts at: 130 1572 130
Size: 1442 3093
SanDI GG'GwC_CC
Cuts at: 1238 1238
Size: 4535
SapI GCTCTTCn'nnn_
Cuts at: 1192 2558 2768 1192
Size: 1366 210 2959
Fragments arranged by size:
2959 1366 210
SbfI CC_TGCA'GG
Cuts at: 578 1582 578
Size: 1004 3531
ScaI AGT'ACT
Cuts at: 4052 4052
Size: 4535
SexAI A'CCwGG_T
Cuts at: 752 752
Size: 4535
SfcI C'TryA_G
Cuts at: 33 150 393 450 574 943 1578 2263 3256
Size: 117 243 57 124 369 635 685 993
Cuts at: 3256 3447 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 635 369 243 191 124
117 57
SfoI GGC'GCC
Cuts at: 1967 2215 1967
Size: 248 4287
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
SmaI CCC'GGG
Cuts at: 1557 1557
Size: 4535
SmlI C'TyrA_G
Cuts at: 330 457 1690 2605 3097 3359 330
Size: 127 1233 915 492 262 1506
Fragments arranged by size:
1506 1233 915 492 262 127
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4535
SphI G_CATG'C
Cuts at: 1588 2088 1588
Size: 500 4035
StyI C'CwwG_G
Cuts at: 815 840 1004 1669 2023 815
Size: 25 164 665 354 3327
Fragments arranged by size:
3327 665 354 164 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 259 2118 2251 2710 2919 259
Size: 1859 133 459 209 1875
Fragments arranged by size:
1875 1859 459 209 133
TatI w'GTAC_w
Cuts at: 386 716 4050 386
Size: 330 3334 871
Fragments arranged by size:
3334 871 330
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 373 405 4052 4084 373
Size: 32 3647 32 824
Fragments arranged by size:
3647 824 32 32
Tth111I GACn'n_nGTC
Cuts at: 2332 2332
Size: 4535
XbaI T'CTAG_A
Cuts at: 1566 1566
Size: 4535
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 813 2030 813
Size: 1217 3318
XhoI C'TCGA_G
Cuts at: 330 330
Size: 4535
XmaI C'CCGG_G
Cuts at: 1555 1555
Size: 4535
XmnI GAAnn'nnTTC
Cuts at: 466 466
Size: 4535
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PpuMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
BaeI BbsI BfrBI BglI BmtI BpmI BsiWI BsmI
BsrGI BssHII BstEII BstZ17I Bsu36I CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
NsiI PacI PflMI PmlI PshAI PsiI PspOMI SacII
SfiI SgrAI SnaBI SrfI SspI StuI SwaI ZraI