Restriction map for
pF1KB9734
(Circular) (Six-base) MAPSORT of: pF1KB9734.seq Check: 5854 from: 1 to: 4571
pF1KB9734 4571 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1316 1316
Size: 4571
AatII G_ACGT'C
Cuts at: 653 653
Size: 4571
AccI GT'mk_AC
Cuts at: 1609 1609
Size: 4571
Acc65I G'GTAC_C
Cuts at: 1587 1587
Size: 4571
AclI AA'CG_TT
Cuts at: 4411 4411
Size: 4571
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 1347 2396 2828 3575 3843 182
Size: 1165 1049 432 747 268 910
Fragments arranged by size:
1165 1049 910 747 432 268
AfeI AGC'GCT
Cuts at: 186 689 186
Size: 503 4068
AflIII A'CryG_T
Cuts at: 1430 1923 3027 4004 1430
Size: 493 1104 977 1997
Fragments arranged by size:
1997 1104 977 493
AgeI A'CCGG_T
Cuts at: 2959 2959
Size: 4571
AhdI GACnn_n'nnGTC
Cuts at: 837 837
Size: 4571
AleI CACnn'nnGTG
Cuts at: 1390 1390
Size: 4571
AlwNI CAG_nnn'CTG
Cuts at: 621 1876 3443 621
Size: 1255 1567 1749
Fragments arranged by size:
1749 1567 1255
ApaI G_GGCC'C
Cuts at: 753 753
Size: 4571
ApaLI G'TGCA_C
Cuts at: 1483 3341 1483
Size: 1858 2713
ApoI r'AATT_y
Cuts at: 1575 3924 4180 1575
Size: 2349 256 1966
Fragments arranged by size:
2349 1966 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4571
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4571
AvaI C'yCGr_G
Cuts at: 924 1591 924
Size: 667 3904
BamHI G'GATC_C
Cuts at: 1596 1596
Size: 4571
BanI G'GyrC_C
Cuts at: 441 604 669 1187 1587 2001 2249 2284 441
Size: 163 65 518 400 414 248 35 2728
Fragments arranged by size:
2728 518 414 400 248 163 65 35
BanII G_rGCy'C
Cuts at: 753 965 1298 1585 2615 753
Size: 212 333 287 1030 2709
Fragments arranged by size:
2709 1030 333 287 212
BbeI G_GCGC'C
Cuts at: 673 1191 2005 2253 673
Size: 518 814 248 2991
Fragments arranged by size:
2991 814 518 248
BbvCI CC'TCA_GC
Cuts at: 346 632 947 1034 346
Size: 286 315 87 3883
Fragments arranged by size:
3883 315 286 87
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4537
BciVI GTATCCnnnnn_n'
Cuts at: 2469 3230 4571 2469
Size: 761 1341 2469
Fragments arranged by size:
2469 1341 761
BclI T'GATC_A
Cuts at: 2091 2091
Size: 4571
BglI GCCn_nnn'nGGC
Cuts at: 796 1069 1196 796
Size: 273 127 4171
Fragments arranged by size:
4171 273 127
BglII A'GATC_T
Cuts at: 2086 4123 2086
Size: 2037 2534
BlpI GC'TnA_GC
Cuts at: 1683 1683
Size: 4571
Bme1580I G_kGCm'C
Cuts at: 383 609 753 1487 2196 2289 3345 383
Size: 226 144 734 709 93 1056 1609
Fragments arranged by size:
1609 1056 734 709 226 144 93
BmgBI CAC'GTC
Cuts at: 1431 1431
Size: 4571
BmrI ACTGGGnnnn_n'
Cuts at: 1783 1864 2199 4343 1783
Size: 81 335 2144 2011
Fragments arranged by size:
2144 2011 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2938 2938
Size: 4571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 334 260
Size: 74 4497
Bpu10I CC'TnA_GC
Cuts at: 118 346 632 947 1034 118
Size: 228 286 315 87 3655
Fragments arranged by size:
3655 315 286 228 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 1474 1504 1747 2626 3118 3416 276
Size: 1198 30 243 879 492 298 1431
Fragments arranged by size:
1431 1198 879 492 298 243 30
BsaI GGTCTCn'nnnn_
Cuts at: 4275 4275
Size: 4571
BsaAI yAC'GTr
Cuts at: 1392 1924 1392
Size: 532 4039
BsaBI GATnn'nnATC
Cuts at: 6 2085 4122 6
Size: 2079 2037 455
Fragments arranged by size:
2079 2037 455
BsaHI Gr'CG_yC
Cuts at: 650 670 1188 2002 2250 2952 4448 650
Size: 20 518 814 248 702 1496 773
Fragments arranged by size:
1496 814 773 702 518 248 20
BsaWI w'CCGG_w
Cuts at: 1760 2281 2959 3233 3380 3791 1760
Size: 521 678 274 147 411 2540
Fragments arranged by size:
2540 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 321 351 526 556 321
Size: 30 175 30 4336
Fragments arranged by size:
4336 175 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 1525 136
Size: 1389 3182
BseYI C'CCAG_C
Cuts at: 181 260 873 892 1047 1542 1996 2754 3331
Size: 79 613 19 155 495 454 758 577
Cuts at: 3331 3827 181
Size: 496 925
Fragments arranged by size:
925 758 613 577 496 495 454 155
79 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1109 1109
Size: 4571
BsiEI CG_ry'CG
Cuts at: 104 869 1231 1440 2113 2159 3367 104
Size: 765 362 209 673 46 1208 1308
Fragments arranged by size:
1308 1208 765 673 362 209 46
BsiHKAI G_wGCw'C
Cuts at: 1298 1487 1585 2363 2553 3345 1298
Size: 189 98 778 190 792 2524
Fragments arranged by size:
2524 792 778 190 189 98
Bsp1286I G_dGCh'C
Cuts at: 383 609 753 965 1298 1487 1585 2196 2289
Size: 226 144 212 333 189 98 611 93
Cuts at: 2289 2363 2553 2615 3345 383
Size: 74 190 62 730 1609
Fragments arranged by size:
1609 730 611 333 226 212 190 189
144 98 93 74 62
BspEI T'CCGG_A
Cuts at: 3791 3791
Size: 4571
BspHI T'CATG_A
Cuts at: 3747 3747
Size: 4571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1316 1621 1835 2137 2518 1316
Size: 305 214 302 381 3369
Fragments arranged by size:
3369 381 305 302 214
BsrBI CCG'CTC
Cuts at: 50 1515 2863 2917 4401 4566 50
Size: 1465 1348 54 1484 165 55
Fragments arranged by size:
1484 1465 1348 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2483 2483
Size: 4571
BsrFI r'CCGG_y
Cuts at: 229 864 1405 2569 2750 2959 229
Size: 635 541 1164 181 209 1841
Fragments arranged by size:
1841 1164 635 541 209 181
BssHII G'CGCG_C
Cuts at: 1282 1450 1501 1503 1282
Size: 168 51 2 4350
Fragments arranged by size:
4350 168 51 2
BssSI C'ACGA_G
Cuts at: 2842 3200 2842
Size: 358 4213
BstAPI GCAn_nnn'nTGC
Cuts at: 1214 1948 1214
Size: 734 3837
BstBI TT'CG_AA
Cuts at: 2932 2932
Size: 4571
BstYI r'GATC_y
Cuts at: 1 475 549 1596 2063 2086 2421 2667 3679
Size: 474 74 1047 467 23 335 246 1012
Cuts at: 3679 3765 3777 4123 1
Size: 86 12 346 449
Fragments arranged by size:
1047 1012 474 467 449 346 335 246
86 74 23 12
BtgI C'CryG_G
Cuts at: 221 1067 1191 1225 221
Size: 846 124 34 3567
Fragments arranged by size:
3567 846 124 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1472 2521 2702 2865 1472
Size: 1049 181 163 3178
Fragments arranged by size:
3178 1049 181 163
BtsI GCAGTG_nn'
Cuts at: 383 622 1119 1814 383
Size: 239 497 695 3140
Fragments arranged by size:
3140 695 497 239
DraI TTT'AAA
Cuts at: 1570 1570
Size: 4571
DrdI GACnn_nn'nnGTC
Cuts at: 2277 3135 2277
Size: 858 3713
EaeI y'GGCC_r
Cuts at: 224 866 1019 1228 1437 2156 2330 2721 2748
Size: 642 153 209 209 719 174 391 27
Cuts at: 2748 4093 224
Size: 1345 702
Fragments arranged by size:
1345 719 702 642 391 209 209 174
153 27
EagI C'GGCC_G
Cuts at: 866 1228 1437 2156 866
Size: 362 209 719 3281
Fragments arranged by size:
3281 719 362 209
EarI CTCTTCn'nnn_
Cuts at: 415 2594 2804 415
Size: 2179 210 2182
Fragments arranged by size:
2182 2179 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 518 1022 1087 1111 2779 3089 3235 4381 518
Size: 504 65 24 1668 310 146 1146 708
Fragments arranged by size:
1668 1146 708 504 310 146 65 24
EcoICRI GAG'CTC
Cuts at: 1296 1583 1296
Size: 287 4284
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 260 334 1347 2396 2828 3575 3843 182
Size: 78 74 1013 1049 432 747 268 910
Fragments arranged by size:
1049 1013 910 747 432 268 78 74
EcoNI CCTnn'n_nnAGG
Cuts at: 242 242
Size: 4571
EcoO109I rG'GnC_Cy
Cuts at: 319 715 749 750 899 951 1524 1710 319
Size: 396 34 1 149 52 573 186 3180
Fragments arranged by size:
3180 573 396 186 149 52 34 1
EcoRI G'AATT_C
Cuts at: 1575 1575
Size: 4571
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2590 2590
Size: 4571
FspI TGC'GCA
Cuts at: 1312 1396 2352 1312
Size: 84 956 3531
Fragments arranged by size:
3531 956 84
FspAI rTGC'GCAy
Cuts at: 1396 1396
Size: 4571
HaeII r_GCGC'y
Cuts at: 188 560 673 691 1064 1191 2005 2253 3275
Size: 372 113 18 373 127 814 248 1022
Cuts at: 3275 4254 188
Size: 979 505
Fragments arranged by size:
1022 979 814 505 373 372 248 127
113 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 526 558 685 717 823 855 2413 2443 2445
Size: 32 127 32 106 32 1558 30 2
Cuts at: 2445 2475 526
Size: 30 2622
Fragments arranged by size:
2622 1558 127 106 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1610 1610
Size: 4571
KasI G'GCGC_C
Cuts at: 669 1187 2001 2249 669
Size: 518 814 248 2991
Fragments arranged by size:
2991 814 518 248
KpnI G_GTAC'C
Cuts at: 1591 1591
Size: 4571
MluI A'CGCG_T
Cuts at: 4004 4004
Size: 4571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1313 3242 3426 1313
Size: 1929 184 2458
Fragments arranged by size:
2458 1929 184
MscI TGG'CCA
Cuts at: 1021 2332 1021
Size: 1311 3260
MslI CAynn'nnrTG
Cuts at: 1390 2560 2687 1390
Size: 1170 127 3274
Fragments arranged by size:
3274 1170 127
MspA1I CmG'CkG
Cuts at: 223 275 356 483 848 896 918 1047 1069
Size: 52 81 127 365 48 22 129 22
Cuts at: 1069 1193 1227 1682 1996 2356 3369 3614 223
Size: 124 34 455 314 360 1013 245 1180
Fragments arranged by size:
1180 1013 455 365 360 314 245 129
127 124 81 52 48 34 22 22
NaeI GCC'GGC
Cuts at: 866 2752 866
Size: 1886 2685
NarI GG'CG_CC
Cuts at: 670 1188 2002 2250 670
Size: 518 814 248 2991
Fragments arranged by size:
2991 814 518 248
NgoMIV G'CCGG_C
Cuts at: 864 2750 864
Size: 1886 2685
NotI GC'GGCC_GC
Cuts at: 1228 1437 1228
Size: 209 4362
NspI r_CATG'y
Cuts at: 1624 2124 3031 1624
Size: 500 907 3164
Fragments arranged by size:
3164 907 500
PasI CC'CwG_GG
Cuts at: 728 754 958 728
Size: 26 204 4341
Fragments arranged by size:
4341 204 26
PciI A'CATG_T
Cuts at: 3027 3027
Size: 4571
PfoI T'CCnGG_A
Cuts at: 311 311
Size: 4571
PmeI GTTT'AAAC
Cuts at: 1570 1570
Size: 4571
PmlI CAC'GTG
Cuts at: 1392 1392
Size: 4571
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 3710
Size: 32 4539
PpuMI rG'GwC_Cy
Cuts at: 715 715
Size: 4571
PspOMI G'GGCC_C
Cuts at: 749 749
Size: 4571
PstI C_TGCA'G
Cuts at: 1618 2303 1618
Size: 685 3886
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4571
PvuII CAG'CTG
Cuts at: 275 848 1996 2356 275
Size: 573 1148 360 2490
Fragments arranged by size:
2490 1148 573 360
RsrII CG'GwC_CG
Cuts at: 2766 2766
Size: 4571
SacI G_AGCT'C
Cuts at: 1298 1585 1298
Size: 287 4284
SacII CC_GC'GG
Cuts at: 224 1070 1194 1228 224
Size: 846 124 34 3567
Fragments arranged by size:
3567 846 124 34
SalI G'TCGA_C
Cuts at: 1608 1608
Size: 4571
SanDI GG'GwC_CC
Cuts at: 715 715
Size: 4571
SapI GCTCTTCn'nnn_
Cuts at: 2594 2804 2594
Size: 210 4361
SbfI CC_TGCA'GG
Cuts at: 1618 1618
Size: 4571
ScaI AGT'ACT
Cuts at: 4088 4088
Size: 4571
SfcI C'TryA_G
Cuts at: 33 351 1614 2299 3292 3483 33
Size: 318 1263 685 993 191 1121
Fragments arranged by size:
1263 1121 993 685 318 191
SfoI GGC'GCC
Cuts at: 671 1189 2003 2251 671
Size: 518 814 248 2991
Fragments arranged by size:
2991 814 518 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4571
SgrAI Cr'CCGG_yG
Cuts at: 1405 1405
Size: 4571
SmaI CCC'GGG
Cuts at: 926 1593 926
Size: 667 3904
SmlI C'TyrA_G
Cuts at: 255 1489 1519 1726 2641 3133 3395 255
Size: 1234 30 207 915 492 262 1431
Fragments arranged by size:
1431 1234 915 492 262 207 30
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4571
SphI G_CATG'C
Cuts at: 1624 2124 1624
Size: 500 4071
StuI AGG'CCT
Cuts at: 437 589 1243 437
Size: 152 654 3765
Fragments arranged by size:
3765 654 152
StyI C'CwwG_G
Cuts at: 1169 1238 1705 2059 1169
Size: 69 467 354 3681
Fragments arranged by size:
3681 467 354 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1414 2154 2287 2746 2955 1414
Size: 740 133 459 209 3030
Fragments arranged by size:
3030 740 459 209 133
TatI w'GTAC_w
Cuts at: 4086 4086
Size: 4571
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 321 353 4088 4120 321
Size: 32 3735 32 772
Fragments arranged by size:
3735 772 32 32
Tth111I GACn'n_nGTC
Cuts at: 697 2368 697
Size: 1671 2900
XbaI T'CTAG_A
Cuts at: 1602 1602
Size: 4571
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 480 578 2066 480
Size: 98 1488 2985
Fragments arranged by size:
2985 1488 98
XmaI C'CCGG_G
Cuts at: 924 1591 924
Size: 667 3904
ZraI GAC'GTC
Cuts at: 651 651
Size: 4571
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NotI NspI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AlfI AloI AscI AvrII BaeI BbsI BfrBI
BmtI BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI HindIII HpaI
MfeI NcoI NdeI NheI NruI NsiI PacI PflMI
PshAI PsiI PspXI PsrI SexAI SfiI SnaBI SrfI
SspI SwaI XhoI XmnI