Restriction map for
pF1KB9735
(Circular) (Six-base) MAPSORT of: pF1KB9735.seq Check: 6881 from: 1 to: 4562
pF1KB9735 4562 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 449 1600 449
Size: 1151 3411
Acc65I G'GTAC_C
Cuts at: 1578 1578
Size: 4562
AclI AA'CG_TT
Cuts at: 4402 4402
Size: 4562
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 520 1466 2387 2819 3566 3834 301
Size: 219 946 921 432 747 268 1029
Fragments arranged by size:
1029 946 921 747 432 268 219
AfeI AGC'GCT
Cuts at: 1117 1117
Size: 4562
AflIII A'CryG_T
Cuts at: 392 1914 3018 3995 392
Size: 1522 1104 977 959
Fragments arranged by size:
1522 1104 977 959
AgeI A'CCGG_T
Cuts at: 2950 2950
Size: 4562
AleI CACnn'nnGTG
Cuts at: 961 961
Size: 4562
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1025 1025
Size: 4562
AlwNI CAG_nnn'CTG
Cuts at: 1281 1309 1384 1867 3434 1281
Size: 28 75 483 1567 2409
Fragments arranged by size:
2409 1567 483 75 28
ApaI G_GGCC'C
Cuts at: 386 386
Size: 4562
ApaLI G'TGCA_C
Cuts at: 3332 3332
Size: 4562
ApoI r'AATT_y
Cuts at: 214 337 1566 3915 4171 214
Size: 123 1229 2349 256 605
Fragments arranged by size:
2349 1229 605 256 123
AseI AT'TA_AT
Cuts at: 20 20
Size: 4562
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4562
AvaI C'yCGr_G
Cuts at: 141 1582 141
Size: 1441 3121
BamHI G'GATC_C
Cuts at: 1587 1587
Size: 4562
BanI G'GyrC_C
Cuts at: 789 1578 1992 2240 2275 789
Size: 789 414 248 35 3076
Fragments arranged by size:
3076 789 414 248 35
BanII G_rGCy'C
Cuts at: 386 1576 2606 386
Size: 1190 1030 2342
Fragments arranged by size:
2342 1190 1030
BbeI G_GCGC'C
Cuts at: 1996 2244 1996
Size: 248 4314
BciVI GTATCCnnnnn_n'
Cuts at: 817 2460 3221 4562 817
Size: 1643 761 1341 817
Fragments arranged by size:
1643 1341 817 761
BclI T'GATC_A
Cuts at: 2082 2082
Size: 4562
BfrBI ATG'CAT
Cuts at: 699 699
Size: 4562
BglII A'GATC_T
Cuts at: 2077 4114 2077
Size: 2037 2525
BlpI GC'TnA_GC
Cuts at: 1046 1401 1674 1046
Size: 355 273 3934
Fragments arranged by size:
3934 355 273
Bme1580I G_kGCm'C
Cuts at: 386 794 2187 2280 3336 386
Size: 408 1393 93 1056 1612
Fragments arranged by size:
1612 1393 1056 408 93
BmrI ACTGGGnnnn_n'
Cuts at: 1073 1260 1297 1536 1774 1855 2190 4334 1073
Size: 187 37 239 238 81 335 2144 1301
Fragments arranged by size:
2144 1301 335 239 238 187 81 37
BmtI G_CTAG'C
Cuts at: 323 323
Size: 4562
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 468 2929 468
Size: 2461 2101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 725 1144 1361 725
Size: 419 217 3926
Fragments arranged by size:
3926 419 217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1313 1738 2617 3109 3407 1313
Size: 425 879 492 298 2468
Fragments arranged by size:
2468 879 492 425 298
BsaI GGTCTCn'nnnn_
Cuts at: 674 1041 4266 674
Size: 367 3225 970
Fragments arranged by size:
3225 970 367
BsaAI yAC'GTr
Cuts at: 1915 1915
Size: 4562
BsaBI GATnn'nnATC
Cuts at: 6 994 1094 2076 4113 6
Size: 988 100 982 2037 455
Fragments arranged by size:
2037 988 982 455 100
BsaHI Gr'CG_yC
Cuts at: 1993 2241 2943 4439 1993
Size: 248 702 1496 2116
Fragments arranged by size:
2116 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1751 2272 2950 3224 3371 3782 1751
Size: 521 678 274 147 411 2531
Fragments arranged by size:
2531 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 249 279 627 657 895 925 1359 1389 249
Size: 30 348 30 238 30 434 30 3422
Fragments arranged by size:
3422 434 348 238 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 307 903 307
Size: 596 3966
BseYI C'CCAG_C
Cuts at: 222 379 1025 1987 2745 3322 3818 222
Size: 157 646 962 758 577 496 966
Fragments arranged by size:
966 962 758 646 577 496 157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1295 1295
Size: 4562
BsiEI CG_ry'CG
Cuts at: 104 2104 2150 3358 104
Size: 2000 46 1208 1308
Fragments arranged by size:
2000 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1576 2354 2544 3336 1576
Size: 778 190 792 2802
Fragments arranged by size:
2802 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 107 107
Size: 4562
Bsp1286I G_dGCh'C
Cuts at: 386 794 1576 2187 2280 2354 2544 2606 3336
Size: 408 782 611 93 74 190 62 730
Cuts at: 3336 386
Size: 1612
Fragments arranged by size:
1612 782 730 611 408 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3782 3782
Size: 4562
BspHI T'CATG_A
Cuts at: 1526 3738 1526
Size: 2212 2350
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1612 1826 2128 2509 1612
Size: 214 302 381 3665
Fragments arranged by size:
3665 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2854 2908 4392 4557 50
Size: 2804 54 1484 165 55
Fragments arranged by size:
2804 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 141 245 1260 1425 2474 141
Size: 104 1015 165 1049 2229
Fragments arranged by size:
2229 1049 1015 165 104
BsrFI r'CCGG_y
Cuts at: 2560 2741 2950 2560
Size: 181 209 4172
Fragments arranged by size:
4172 209 181
BssSI C'ACGA_G
Cuts at: 2833 3191 2833
Size: 358 4204
BstAPI GCAn_nnn'nTGC
Cuts at: 1939 1939
Size: 4562
BstBI TT'CG_AA
Cuts at: 2923 2923
Size: 4562
BstEII G'GTnAC_C
Cuts at: 513 513
Size: 4562
BstXI CCAn_nnnn'nTGG
Cuts at: 960 960
Size: 4562
BstYI r'GATC_y
Cuts at: 1 1095 1587 2054 2077 2412 2658 3670 3756
Size: 1094 492 467 23 335 246 1012 86
Cuts at: 3756 3768 4114 1
Size: 12 346 449
Fragments arranged by size:
1094 1012 492 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 785 959 107
Size: 678 174 3710
Fragments arranged by size:
3710 678 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2512 2693 2856 2512
Size: 181 163 4218
Fragments arranged by size:
4218 181 163
BtsI GCAGTG_nn'
Cuts at: 1307 1805 1307
Size: 498 4064
DraI TTT'AAA
Cuts at: 1561 1561
Size: 4562
DrdI GACnn_nn'nnGTC
Cuts at: 1303 1536 2268 3126 1303
Size: 233 732 858 2739
Fragments arranged by size:
2739 858 732 233
EaeI y'GGCC_r
Cuts at: 770 852 1454 1478 2147 2321 2712 2739 4084
Size: 82 602 24 669 174 391 27 1345
Cuts at: 4084 770
Size: 1248
Fragments arranged by size:
1345 1248 669 602 391 174 82 27
24
EagI C'GGCC_G
Cuts at: 2147 2147
Size: 4562
EarI CTCTTCn'nnn_
Cuts at: 310 428 2585 2795 310
Size: 118 2157 210 2077
Fragments arranged by size:
2157 2077 210 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2770 3080 3226 4372 2770
Size: 310 146 1146 2960
Fragments arranged by size:
2960 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1574 1574
Size: 4562
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 520 725 1144 1361 1466 2387 2819 3566
Size: 219 205 419 217 105 921 432 747
Cuts at: 3566 3834 301
Size: 268 1029
Fragments arranged by size:
1029 921 747 432 419 268 219 217
205 105
EcoO109I rG'GnC_Cy
Cuts at: 383 736 1701 383
Size: 353 965 3244
Fragments arranged by size:
3244 965 353
EcoRI G'AATT_C
Cuts at: 337 1566 337
Size: 1229 3333
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2581 2581
Size: 4562
FspI TGC'GCA
Cuts at: 2343 2343
Size: 4562
HaeII r_GCGC'y
Cuts at: 1119 1996 2244 3266 4245 1119
Size: 877 248 1022 979 1436
Fragments arranged by size:
1436 1022 979 877 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1000 1032 2404 2434 2436 2466 1000
Size: 32 1372 30 2 30 3096
Fragments arranged by size:
3096 1372 32 30 30 2
HincII GTy'rAC
Cuts at: 825 1601 825
Size: 776 3786
HpaI GTT'AAC
Cuts at: 825 825
Size: 4562
KasI G'GCGC_C
Cuts at: 1992 2240 1992
Size: 248 4314
KpnI G_GTAC'C
Cuts at: 1582 1582
Size: 4562
MluI A'CGCG_T
Cuts at: 3995 3995
Size: 4562
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 292 631 677 3233 3417 292
Size: 339 46 2556 184 1437
Fragments arranged by size:
2556 1437 339 184 46
MscI TGG'CCA
Cuts at: 772 854 1456 1480 2323 772
Size: 82 602 24 843 3011
Fragments arranged by size:
3011 843 602 82 24
MslI CAynn'nnrTG
Cuts at: 197 724 961 1007 1474 2551 2678 197
Size: 527 237 46 467 1077 127 2081
Fragments arranged by size:
2081 1077 527 467 237 127 46
MspA1I CmG'CkG
Cuts at: 1151 1227 1444 1673 1987 2347 3360 3605 1151
Size: 76 217 229 314 360 1013 245 2108
Fragments arranged by size:
2108 1013 360 314 245 229 217 76
NaeI GCC'GGC
Cuts at: 2743 2743
Size: 4562
NarI GG'CG_CC
Cuts at: 1993 2241 1993
Size: 248 4314
NcoI C'CATG_G
Cuts at: 107 785 959 107
Size: 678 174 3710
Fragments arranged by size:
3710 678 174
NgoMIV G'CCGG_C
Cuts at: 2741 2741
Size: 4562
NheI G'CTAG_C
Cuts at: 319 319
Size: 4562
NsiI A_TGCA'T
Cuts at: 701 701
Size: 4562
NspI r_CATG'y
Cuts at: 396 1506 1522 1615 2115 3022 396
Size: 1110 16 93 500 907 1936
Fragments arranged by size:
1936 1110 907 500 93 16
PciI A'CATG_T
Cuts at: 392 3018 392
Size: 2626 1936
PmeI GTTT'AAAC
Cuts at: 1561 1561
Size: 4562
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3701 3733 3701
Size: 32 4530
PshAI GACnn'nnGTC
Cuts at: 780 780
Size: 4562
PspOMI G'GGCC_C
Cuts at: 382 382
Size: 4562
PstI C_TGCA'G
Cuts at: 354 1143 1443 1609 2294 354
Size: 789 300 166 685 2622
Fragments arranged by size:
2622 789 685 300 166
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4562
PvuII CAG'CTG
Cuts at: 1227 1444 1987 2347 1227
Size: 217 543 360 3442
Fragments arranged by size:
3442 543 360 217
RsrII CG'GwC_CG
Cuts at: 2757 2757
Size: 4562
SacI G_AGCT'C
Cuts at: 1576 1576
Size: 4562
SalI G'TCGA_C
Cuts at: 1599 1599
Size: 4562
SapI GCTCTTCn'nnn_
Cuts at: 428 2585 2795 428
Size: 2157 210 2195
Fragments arranged by size:
2195 2157 210
SbfI CC_TGCA'GG
Cuts at: 1609 1609
Size: 4562
ScaI AGT'ACT
Cuts at: 1062 4079 1062
Size: 3017 1545
SfcI C'TryA_G
Cuts at: 33 350 1139 1315 1439 1605 2290 3283 3474
Size: 317 789 176 124 166 685 993 191
Cuts at: 3474 33
Size: 1121
Fragments arranged by size:
1121 993 789 685 317 191 176 166
124
SfoI GGC'GCC
Cuts at: 1994 2242 1994
Size: 248 4314
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4562
SmaI CCC'GGG
Cuts at: 143 1584 143
Size: 1441 3121
SmlI C'TyrA_G
Cuts at: 1328 1717 2632 3124 3386 1328
Size: 389 915 492 262 2504
Fragments arranged by size:
2504 915 492 389 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4562
SphI G_CATG'C
Cuts at: 1506 1615 2115 1506
Size: 109 500 3953
Fragments arranged by size:
3953 500 109
StyI C'CwwG_G
Cuts at: 107 785 959 1349 1696 2050 107
Size: 678 174 390 347 354 2619
Fragments arranged by size:
2619 678 390 354 347 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 564 1164 1452 2145 2278 2737 2946 564
Size: 600 288 693 133 459 209 2180
Fragments arranged by size:
2180 693 600 459 288 209 133
TatI w'GTAC_w
Cuts at: 744 1060 4077 744
Size: 316 3017 1229
Fragments arranged by size:
3017 1229 316
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 627 659 895 926 927 958 4079 4111 627
Size: 32 236 31 1 31 3121 32 1078
Fragments arranged by size:
3121 1078 236 32 32 31 31 1
Tth111I GACn'n_nGTC
Cuts at: 238 2359 238
Size: 2121 2441
XbaI T'CTAG_A
Cuts at: 1593 1593
Size: 4562
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 660 2057 660
Size: 1397 3165
XmaI C'CCGG_G
Cuts at: 141 1582 141
Size: 1441 3121
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI
NspI PciI PmeI PpiI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI AscI AvrII BaeI
BbsI BbvCI BcgI BglI BmgBI Bpu10I BsiWI BsmI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII MfeI NdeI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI