Restriction map for
pF1KB9738
(Circular) (Six-base) MAPSORT of: pF1KB9738.seq Check: 2051 from: 1 to: 4622
pF1KB9738 4622 bp
With 182 enzymes: SgfI *
March 5, 2009 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 302 302
Size: 4622
AccI GT'mk_AC
Cuts at: 1660 1660
Size: 4622
Acc65I G'GTAC_C
Cuts at: 1638 1638
Size: 4622
AclI AA'CG_TT
Cuts at: 4462 4462
Size: 4622
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1311 2447 2879 3626 3894 1311
Size: 1136 432 747 268 2039
Fragments arranged by size:
2039 1136 747 432 268
AflIII A'CryG_T
Cuts at: 138 412 1974 3078 4055 138
Size: 274 1562 1104 977 705
Fragments arranged by size:
1562 1104 977 705 274
AgeI A'CCGG_T
Cuts at: 3010 3010
Size: 4622
AleI CACnn'nnGTG
Cuts at: 946 1203 946
Size: 257 4365
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 964 1024 964
Size: 60 4562
AlwNI CAG_nnn'CTG
Cuts at: 182 204 1419 1547 1927 3494 182
Size: 22 1215 128 380 1567 1310
Fragments arranged by size:
1567 1310 1215 380 128 22
ApaI G_GGCC'C
Cuts at: 569 851 1212 569
Size: 282 361 3979
Fragments arranged by size:
3979 361 282
ApaLI G'TGCA_C
Cuts at: 1486 3392 1486
Size: 1906 2716
ApoI r'AATT_y
Cuts at: 1354 1626 3975 4231 1354
Size: 272 2349 256 1745
Fragments arranged by size:
2349 1745 272 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4622
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4622
AvaI C'yCGr_G
Cuts at: 349 441 844 1029 1642 349
Size: 92 403 185 613 3329
Fragments arranged by size:
3329 613 403 185 92
BamHI G'GATC_C
Cuts at: 1647 1647
Size: 4622
BanI G'GyrC_C
Cuts at: 1638 2052 2300 2335 1638
Size: 414 248 35 3925
Fragments arranged by size:
3925 414 248 35
BanII G_rGCy'C
Cuts at: 569 797 851 959 1118 1212 1636 2666 569
Size: 228 54 108 159 94 424 1030 2525
Fragments arranged by size:
2525 1030 424 228 159 108 94 54
BbeI G_GCGC'C
Cuts at: 2056 2304 2056
Size: 248 4374
BbsI GAAGACnn'nnnn_
Cuts at: 1326 1511 1326
Size: 185 4437
BbvCI CC'TCA_GC
Cuts at: 1540 1540
Size: 4622
BciVI GTATCCnnnnn_n'
Cuts at: 2520 3281 4622 2520
Size: 761 1341 2520
Fragments arranged by size:
2520 1341 761
BclI T'GATC_A
Cuts at: 2142 2142
Size: 4622
BglI GCCn_nnn'nGGC
Cuts at: 573 573
Size: 4622
BglII A'GATC_T
Cuts at: 2137 4174 2137
Size: 2037 2585
BlpI GC'TnA_GC
Cuts at: 478 1734 478
Size: 1256 3366
Bme1580I G_kGCm'C
Cuts at: 236 569 851 1212 1365 1490 1501 2247 2340
Size: 333 282 361 153 125 11 746 93
Cuts at: 2340 3396 236
Size: 1056 1462
Fragments arranged by size:
1462 1056 746 361 333 282 153 125
93 11
BmgBI CAC'GTC
Cuts at: 978 978
Size: 4622
BmrI ACTGGGnnnn_n'
Cuts at: 779 1248 1834 1915 2250 4394 779
Size: 469 586 81 335 2144 1007
Fragments arranged by size:
2144 1007 586 469 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 175 2989 175
Size: 2814 1808
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 643 761 803 1204 1624 643
Size: 118 42 401 420 3641
Fragments arranged by size:
3641 420 401 118 42
Bpu10I CC'TnA_GC
Cuts at: 262 1540 262
Size: 1278 3344
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1798 2677 3169 3467 1798
Size: 879 492 298 2953
Fragments arranged by size:
2953 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4326 4326
Size: 4622
BsaAI yAC'GTr
Cuts at: 1164 1975 1164
Size: 811 3811
BsaBI GATnn'nnATC
Cuts at: 6 2136 4173 6
Size: 2130 2037 455
Fragments arranged by size:
2130 2037 455
BsaHI Gr'CG_yC
Cuts at: 2053 2301 3003 4499 2053
Size: 248 702 1496 2176
Fragments arranged by size:
2176 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1811 2332 3010 3284 3431 3842 1811
Size: 521 678 274 147 411 2591
Fragments arranged by size:
2591 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 720 750 720
Size: 30 4592
BseYI C'CCAG_C
Cuts at: 630 802 838 851 958 1062 1085 1102 1281
Size: 172 36 13 107 104 23 17 179
Cuts at: 1281 1426 2047 2805 3382 3878 630
Size: 145 621 758 577 496 1374
Fragments arranged by size:
1374 758 621 577 496 179 172 145
107 104 36 23 17 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 924 1020 924
Size: 96 4526
BsiEI CG_ry'CG
Cuts at: 104 455 2164 2210 3418 104
Size: 351 1709 46 1208 1308
Fragments arranged by size:
1709 1308 1208 351 46
BsiHKAI G_wGCw'C
Cuts at: 1118 1490 1636 2414 2604 3396 1118
Size: 372 146 778 190 792 2344
Fragments arranged by size:
2344 792 778 372 190 146
BsmI GAATG_Cn'
Cuts at: 1096 1096
Size: 4622
BsmBI CGTCTCn'nnnn_
Cuts at: 823 823
Size: 4622
Bsp1286I G_dGCh'C
Cuts at: 236 569 797 851 959 1118 1212 1365 1490
Size: 333 228 54 108 159 94 153 125
Cuts at: 1490 1501 1636 2247 2340 2414 2604 2666 3396
Size: 11 135 611 93 74 190 62 730
Cuts at: 3396 236
Size: 1462
Fragments arranged by size:
1462 730 611 333 228 190 159 153
135 125 108 94 93 74 62 54
11
BspEI T'CCGG_A
Cuts at: 3842 3842
Size: 4622
BspHI T'CATG_A
Cuts at: 3798 3798
Size: 4622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 302 1080 1672 1886 2188 2569 302
Size: 778 592 214 302 381 2355
Fragments arranged by size:
2355 778 592 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2914 2968 4452 4617 50
Size: 2864 54 1484 165 55
Fragments arranged by size:
2864 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2534 2534
Size: 4622
BsrFI r'CCGG_y
Cuts at: 373 2620 2801 3010 373
Size: 2247 181 209 1985
Fragments arranged by size:
2247 1985 209 181
BsrGI T'GTAC_A
Cuts at: 989 989
Size: 4622
BssHII G'CGCG_C
Cuts at: 250 277 369 250
Size: 27 92 4503
Fragments arranged by size:
4503 92 27
BssSI C'ACGA_G
Cuts at: 2893 3251 2893
Size: 358 4264
BstAPI GCAn_nnn'nTGC
Cuts at: 182 1999 182
Size: 1817 2805
BstBI TT'CG_AA
Cuts at: 2983 2983
Size: 4622
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1205 1401 114
Size: 1091 196 3335
Fragments arranged by size:
3335 1091 196
BstYI r'GATC_y
Cuts at: 1 717 1647 2114 2137 2472 2718 3730 3816
Size: 716 930 467 23 335 246 1012 86
Cuts at: 3816 3828 4174 1
Size: 12 346 449
Fragments arranged by size:
1012 930 716 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 306 857 881 944 1374 107
Size: 199 551 24 63 430 3355
Fragments arranged by size:
3355 551 430 199 63 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2572 2753 2916 2572
Size: 181 163 4278
Fragments arranged by size:
4278 181 163
BtsI GCAGTG_nn'
Cuts at: 205 400 671 1865 205
Size: 195 271 1194 2962
Fragments arranged by size:
2962 1194 271 195
DraI TTT'AAA
Cuts at: 1621 1621
Size: 4622
DraIII CAC_nnn'GTG
Cuts at: 468 562 468
Size: 94 4528
DrdI GACnn_nn'nnGTC
Cuts at: 1066 1330 2328 3186 1066
Size: 264 998 858 2502
Fragments arranged by size:
2502 998 858 264
EaeI y'GGCC_r
Cuts at: 316 452 2207 2381 2772 2799 4144 316
Size: 136 1755 174 391 27 1345 794
Fragments arranged by size:
1755 1345 794 391 174 136 27
EagI C'GGCC_G
Cuts at: 452 2207 452
Size: 1755 2867
EarI CTCTTCn'nnn_
Cuts at: 1438 2645 2855 1438
Size: 1207 210 3205
Fragments arranged by size:
3205 1207 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1254 1265 2830 3140 3286 4432 1254
Size: 11 1565 310 146 1146 1444
Fragments arranged by size:
1565 1444 1146 310 146 11
EcoICRI GAG'CTC
Cuts at: 1116 1634 1116
Size: 518 4104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 643 761 803 1204 1311 1624 2447 2879 3626
Size: 118 42 401 107 313 823 432 747
Cuts at: 3626 3894 643
Size: 268 1371
Fragments arranged by size:
1371 823 747 432 401 313 268 118
107 42
EcoNI CCTnn'n_nnAGG
Cuts at: 265 658 265
Size: 393 4229
EcoO109I rG'GnC_Cy
Cuts at: 258 447 534 566 616 648 773 848 1416
Size: 189 87 32 50 32 125 75 568
Cuts at: 1416 1761 258
Size: 345 3119
Fragments arranged by size:
3119 568 345 189 125 87 75 50
32 32
EcoRI G'AATT_C
Cuts at: 1354 1626 1354
Size: 272 4350
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 775 2641 775
Size: 1866 2756
FspI TGC'GCA
Cuts at: 399 406 2403 399
Size: 7 1997 2618
Fragments arranged by size:
2618 1997 7
HaeII r_GCGC'y
Cuts at: 938 973 1374 1562 2056 2304 3326 4305 938
Size: 35 401 188 494 248 1022 979 1255
Fragments arranged by size:
1255 1022 979 494 401 248 188 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1501 1533 2464 2494 2496 2526 1501
Size: 32 931 30 2 30 3597
Fragments arranged by size:
3597 931 32 30 30 2
HincII GTy'rAC
Cuts at: 1661 1661
Size: 4622
KasI G'GCGC_C
Cuts at: 2052 2300 2052
Size: 248 4374
KpnI G_GTAC'C
Cuts at: 1642 1642
Size: 4622
MluI A'CGCG_T
Cuts at: 138 412 4055 138
Size: 274 3643 705
Fragments arranged by size:
3643 705 274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3293 3477 3293
Size: 184 4438
MscI TGG'CCA
Cuts at: 2383 2383
Size: 4622
MslI CAynn'nnrTG
Cuts at: 593 946 1203 2611 2738 593
Size: 353 257 1408 127 2477
Fragments arranged by size:
2477 1408 353 257 127
MspA1I CmG'CkG
Cuts at: 179 308 395 544 634 859 883 946 1066
Size: 129 87 149 90 225 24 63 120
Cuts at: 1066 1134 1203 1426 1733 2047 2407 3420 3665
Size: 68 69 223 307 314 360 1013 245
Cuts at: 3665 179
Size: 1136
Fragments arranged by size:
1136 1013 360 314 307 245 225 223
149 129 120 90 87 69 68 63
24
NaeI GCC'GGC
Cuts at: 375 2803 375
Size: 2428 2194
NarI GG'CG_CC
Cuts at: 2053 2301 2053
Size: 248 4374
NcoI C'CATG_G
Cuts at: 107 107
Size: 4622
NgoMIV G'CCGG_C
Cuts at: 373 2801 373
Size: 2428 2194
NspI r_CATG'y
Cuts at: 598 1675 2175 3082 598
Size: 1077 500 907 2138
Fragments arranged by size:
2138 1077 907 500
PasI CC'CwG_GG
Cuts at: 571 790 964 1171 571
Size: 219 174 207 4022
Fragments arranged by size:
4022 219 207 174
PciI A'CATG_T
Cuts at: 3078 3078
Size: 4622
PflMI CCAn_nnn'nTGG
Cuts at: 562 1347 562
Size: 785 3837
PfoI T'CCnGG_A
Cuts at: 738 1118 738
Size: 380 4242
PmeI GTTT'AAAC
Cuts at: 1621 1621
Size: 4622
PmlI CAC'GTG
Cuts at: 1164 1164
Size: 4622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 3761
Size: 32 4590
PpuMI rG'GwC_Cy
Cuts at: 258 447 648 1416 258
Size: 189 201 768 3464
Fragments arranged by size:
3464 768 201 189
PspOMI G'GGCC_C
Cuts at: 565 847 1208 565
Size: 282 361 3979
Fragments arranged by size:
3979 361 282
PstI C_TGCA'G
Cuts at: 200 1013 1669 2354 200
Size: 813 656 685 2468
Fragments arranged by size:
2468 813 685 656
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4622
PvuII CAG'CTG
Cuts at: 544 634 1066 1134 1203 1426 2047 2407 544
Size: 90 432 68 69 223 621 360 2759
Fragments arranged by size:
2759 621 432 360 223 90 69 68
RsrII CG'GwC_CG
Cuts at: 2817 2817
Size: 4622
SacI G_AGCT'C
Cuts at: 1118 1636 1118
Size: 518 4104
SacII CC_GC'GG
Cuts at: 309 860 884 947 309
Size: 551 24 63 3984
Fragments arranged by size:
3984 551 63 24
SalI G'TCGA_C
Cuts at: 1659 1659
Size: 4622
SanDI GG'GwC_CC
Cuts at: 447 447
Size: 4622
SapI GCTCTTCn'nnn_
Cuts at: 1438 2645 2855 1438
Size: 1207 210 3205
Fragments arranged by size:
3205 1207 210
SbfI CC_TGCA'GG
Cuts at: 1669 1669
Size: 4622
ScaI AGT'ACT
Cuts at: 4139 4139
Size: 4622
SfcI C'TryA_G
Cuts at: 33 196 1009 1665 2350 3343 3534 33
Size: 163 813 656 685 993 191 1121
Fragments arranged by size:
1121 993 813 685 656 191 163
SfiI GGCCn_nnn'nGGCC
Cuts at: 573 573
Size: 4622
SfoI GGC'GCC
Cuts at: 2054 2302 2054
Size: 248 4374
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4622
SmaI CCC'GGG
Cuts at: 846 1644 846
Size: 798 3824
SmlI C'TyrA_G
Cuts at: 1777 2692 3184 3446 1777
Size: 915 492 262 2953
Fragments arranged by size:
2953 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4622
SphI G_CATG'C
Cuts at: 1675 2175 1675
Size: 500 4122
StuI AGG'CCT
Cuts at: 292 292
Size: 4622
StyI C'CwwG_G
Cuts at: 107 113 162 731 1436 1756 2110 107
Size: 6 49 569 705 320 354 2619
Fragments arranged by size:
2619 705 569 354 320 49 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2205 2338 2797 3006 2205
Size: 133 459 209 3821
Fragments arranged by size:
3821 459 209 133
TatI w'GTAC_w
Cuts at: 989 1235 4137 989
Size: 246 2902 1474
Fragments arranged by size:
2902 1474 246
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4139 4171 4139
Size: 32 4590
Tth111I GACn'n_nGTC
Cuts at: 702 2419 702
Size: 1717 2905
XbaI T'CTAG_A
Cuts at: 1653 1653
Size: 4622
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2117 2117
Size: 4622
XmaI C'CCGG_G
Cuts at: 844 1642 844
Size: 798 3824
XmnI GAAnn'nnTTC
Cuts at: 244 758 244
Size: 514 4108
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BcgI BfrBI BmtI BseRI BsiWI BstEII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PshAI PsiI
PspXI PsrI SexAI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI