Restriction map for
pF1KB9744
(Circular) (Six-base) MAPSORT of: pF1KB9744.seq Check: 6587 from: 1 to: 4709
pF1KB9744 4709 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1477 1477
Size: 4709
AatII G_ACGT'C
Cuts at: 1631 1631
Size: 4709
AccI GT'mk_AC
Cuts at: 1747 1747
Size: 4709
Acc65I G'GTAC_C
Cuts at: 1725 1725
Size: 4709
AclI AA'CG_TT
Cuts at: 4549 4549
Size: 4709
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 685 769 955 997 2534 2966 3713 3981
Size: 90 84 186 42 1537 432 747 268
Cuts at: 3981 595
Size: 1323
Fragments arranged by size:
1537 1323 747 432 268 186 90 84
42
AflIII A'CryG_T
Cuts at: 2061 3165 4142 2061
Size: 1104 977 2628
Fragments arranged by size:
2628 1104 977
AgeI A'CCGG_T
Cuts at: 3097 3097
Size: 4709
AleI CACnn'nnGTG
Cuts at: 1675 1675
Size: 4709
AlwNI CAG_nnn'CTG
Cuts at: 555 1158 1270 2014 3581 555
Size: 603 112 744 1567 1683
Fragments arranged by size:
1683 1567 744 603 112
ApaI G_GGCC'C
Cuts at: 693 1087 693
Size: 394 4315
ApaLI G'TGCA_C
Cuts at: 877 3479 877
Size: 2602 2107
ApoI r'AATT_y
Cuts at: 1713 4062 4318 1713
Size: 2349 256 2104
Fragments arranged by size:
2349 2104 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4709
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4709
AvaI C'yCGr_G
Cuts at: 261 509 568 964 1204 1729 261
Size: 248 59 396 240 525 3241
Fragments arranged by size:
3241 525 396 248 240 59
BamHI G'GATC_C
Cuts at: 579 1734 579
Size: 1155 3554
BanI G'GyrC_C
Cuts at: 1102 1113 1124 1365 1725 2139 2387 2422 1102
Size: 11 11 241 360 414 248 35 3389
Fragments arranged by size:
3389 414 360 248 241 35 11 11
BanII G_rGCy'C
Cuts at: 693 871 1015 1087 1723 2753 693
Size: 178 144 72 636 1030 2649
Fragments arranged by size:
2649 1030 636 178 144 72
BbeI G_GCGC'C
Cuts at: 1369 2143 2391 1369
Size: 774 248 3687
Fragments arranged by size:
3687 774 248
BbsI GAAGACnn'nnnn_
Cuts at: 132 645 673 132
Size: 513 28 4168
Fragments arranged by size:
4168 513 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1587 1615 1621 1649 1587
Size: 28 6 28 4647
Fragments arranged by size:
4647 28 28 6
BciVI GTATCCnnnnn_n'
Cuts at: 2607 3368 4709 2607
Size: 761 1341 2607
Fragments arranged by size:
2607 1341 761
BclI T'GATC_A
Cuts at: 2229 2229
Size: 4709
BglI GCCn_nnn'nGGC
Cuts at: 766 851 1309 1459 766
Size: 85 458 150 4016
Fragments arranged by size:
4016 458 150 85
BglII A'GATC_T
Cuts at: 2224 4261 2224
Size: 2037 2672
BlpI GC'TnA_GC
Cuts at: 118 1159 1821 118
Size: 1041 662 3006
Fragments arranged by size:
3006 1041 662
Bme1580I G_kGCm'C
Cuts at: 693 881 1087 1129 1282 2334 2427 3483 693
Size: 188 206 42 153 1052 93 1056 1919
Fragments arranged by size:
1919 1056 1052 206 188 153 93 42
BmgBI CAC'GTC
Cuts at: 449 449
Size: 4709
BmrI ACTGGGnnnn_n'
Cuts at: 523 776 1292 1412 1921 2002 2337 4481 523
Size: 253 516 120 509 81 335 2144 751
Fragments arranged by size:
2144 751 516 509 335 253 120 81
BmtI G_CTAG'C
Cuts at: 1351 1351
Size: 4709
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3076 3076
Size: 4709
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 1001 1150 208
Size: 793 149 3767
Fragments arranged by size:
3767 793 149
Bpu10I CC'TnA_GC
Cuts at: 160 160
Size: 4709
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 1885 2764 3256 3554 376
Size: 1509 879 492 298 1531
Fragments arranged by size:
1531 1509 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 514 1219 4413 514
Size: 705 3194 810
Fragments arranged by size:
3194 810 705
BsaAI yAC'GTr
Cuts at: 1614 2062 1614
Size: 448 4261
BsaBI GATnn'nnATC
Cuts at: 6 2223 4260 6
Size: 2217 2037 455
Fragments arranged by size:
2217 2037 455
BsaHI Gr'CG_yC
Cuts at: 1366 1628 2140 2388 3090 4586 1366
Size: 262 512 248 702 1496 1489
Fragments arranged by size:
1496 1489 702 512 262 248
BsaWI w'CCGG_w
Cuts at: 314 587 890 1898 2419 3097 3371 3518 3929
Size: 273 303 1008 521 678 274 147 411
Cuts at: 3929 314
Size: 1094
Fragments arranged by size:
1094 1008 678 521 411 303 274 273
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1507 1537 1507
Size: 30 4679
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 259 426 447 1507 127
Size: 132 167 21 1060 3329
Fragments arranged by size:
3329 1060 167 132 21
BseYI C'CCAG_C
Cuts at: 233 1002 1042 1120 1136 1263 1537 2134 2892
Size: 769 40 78 16 127 274 597 758
Cuts at: 2892 3469 3965 233
Size: 577 496 977
Fragments arranged by size:
977 769 758 597 577 496 274 127
78 40 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 328
Size: 4709
BsiEI CG_ry'CG
Cuts at: 104 846 2251 2297 3505 104
Size: 742 1405 46 1208 1308
Fragments arranged by size:
1405 1308 1208 742 46
BsiHKAI G_wGCw'C
Cuts at: 871 881 1613 1723 2501 2691 3483 871
Size: 10 732 110 778 190 792 2097
Fragments arranged by size:
2097 792 778 732 190 110 10
Bsp1286I G_dGCh'C
Cuts at: 693 871 881 1015 1087 1129 1282 1613 1723
Size: 178 10 134 72 42 153 331 110
Cuts at: 1723 2334 2427 2501 2691 2753 3483 693
Size: 611 93 74 190 62 730 1919
Fragments arranged by size:
1919 730 611 331 190 178 153 134
110 93 74 72 62 42 10
BspEI T'CCGG_A
Cuts at: 587 3929 587
Size: 3342 1367
BspHI T'CATG_A
Cuts at: 3885 3885
Size: 4709
BspMI ACCTGCnnnn'nnnn_
Cuts at: 126 914 1477 1759 1973 2275 2656 126
Size: 788 563 282 214 302 381 2179
Fragments arranged by size:
2179 788 563 381 302 282 214
BsrBI CCG'CTC
Cuts at: 50 3001 3055 4539 4704 50
Size: 2951 54 1484 165 55
Fragments arranged by size:
2951 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2621 2621
Size: 4709
BsrFI r'CCGG_y
Cuts at: 341 377 2707 2888 3097 341
Size: 36 2330 181 209 1953
Fragments arranged by size:
2330 1953 209 181 36
BssSI C'ACGA_G
Cuts at: 2980 3338 2980
Size: 358 4351
BstAPI GCAn_nnn'nTGC
Cuts at: 2086 2086
Size: 4709
BstBI TT'CG_AA
Cuts at: 3070 3070
Size: 4709
BstXI CCAn_nnnn'nTGG
Cuts at: 789 927 1362 1677 789
Size: 138 435 315 3821
Fragments arranged by size:
3821 435 315 138
BstYI r'GATC_y
Cuts at: 1 551 579 681 1734 2201 2224 2559 2805
Size: 550 28 102 1053 467 23 335 246
Cuts at: 2805 3817 3903 3915 4261 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1053 1012 550 467 449 346 335 246
102 86 28 23 12
Bsu36I CC'TnA_GG
Cuts at: 1693 1693
Size: 4709
BtgI C'CryG_G
Cuts at: 107 788 1098 107
Size: 681 310 3718
Fragments arranged by size:
3718 681 310
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2659 2840 3003 2659
Size: 181 163 4365
Fragments arranged by size:
4365 181 163
BtsI GCAGTG_nn'
Cuts at: 356 1367 1413 1468 1631 1952 356
Size: 1011 46 55 163 321 3113
Fragments arranged by size:
3113 1011 321 163 55 46
DraI TTT'AAA
Cuts at: 1708 1708
Size: 4709
DraIII CAC_nnn'GTG
Cuts at: 531 531
Size: 4709
DrdI GACnn_nn'nnGTC
Cuts at: 2415 3273 2415
Size: 858 3851
EaeI y'GGCC_r
Cuts at: 343 560 653 695 791 809 830 843 1253
Size: 217 93 42 96 18 21 13 410
Cuts at: 1253 1463 2294 2468 2859 2886 4231 343
Size: 210 831 174 391 27 1345 821
Fragments arranged by size:
1345 831 821 410 391 217 210 174
96 93 42 27 21 18 13
EagI C'GGCC_G
Cuts at: 843 2294 843
Size: 1451 3258
EarI CTCTTCn'nnn_
Cuts at: 313 444 465 467 2732 2942 313
Size: 131 21 2 2265 210 2080
Fragments arranged by size:
2265 2080 210 131 21 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1515 2917 3227 3373 4519 1515
Size: 1402 310 146 1146 1705
Fragments arranged by size:
1705 1402 1146 310 146
EcoICRI GAG'CTC
Cuts at: 869 1721 869
Size: 852 3857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 595 685 769 955 997 1001 1150 2534
Size: 387 90 84 186 42 4 149 1384
Cuts at: 2534 2966 3713 3981 208
Size: 432 747 268 936
Fragments arranged by size:
1384 936 747 432 387 268 186 149
90 84 42 4
EcoNI CCTnn'n_nnAGG
Cuts at: 655 820 655
Size: 165 4544
EcoO109I rG'GnC_Cy
Cuts at: 505 689 690 1038 1083 1084 1132 1848 505
Size: 184 1 348 45 1 48 716 3366
Fragments arranged by size:
3366 716 348 184 48 45 1 1
EcoRI G'AATT_C
Cuts at: 1713 1713
Size: 4709
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2728 2728
Size: 4709
FseI GG_CCGG'CC
Cuts at: 345 345
Size: 4709
FspI TGC'GCA
Cuts at: 2490 2490
Size: 4709
HaeII r_GCGC'y
Cuts at: 384 725 934 1369 2143 2391 3413 4392 384
Size: 341 209 435 774 248 1022 979 701
Fragments arranged by size:
1022 979 774 701 435 341 248 209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 394 426 1507 1513 1539 1545 2551 2581 2583
Size: 32 1081 6 26 6 1006 30 2
Cuts at: 2583 2613 394
Size: 30 2490
Fragments arranged by size:
2490 1081 1006 32 30 30 26 6
6 2
HincII GTy'rAC
Cuts at: 1748 1748
Size: 4709
KasI G'GCGC_C
Cuts at: 1365 2139 2387 1365
Size: 774 248 3687
Fragments arranged by size:
3687 774 248
KpnI G_GTAC'C
Cuts at: 1729 1729
Size: 4709
MluI A'CGCG_T
Cuts at: 4142 4142
Size: 4709
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1538 3380 3564 1538
Size: 1842 184 2683
Fragments arranged by size:
2683 1842 184
MscI TGG'CCA
Cuts at: 562 655 793 811 832 2470 562
Size: 93 138 18 21 1638 2801
Fragments arranged by size:
2801 1638 138 93 21 18
MslI CAynn'nnrTG
Cuts at: 361 1097 1675 2698 2825 361
Size: 736 578 1023 127 2245
Fragments arranged by size:
2245 1023 736 578 127
MspA1I CmG'CkG
Cuts at: 233 860 1046 1307 1820 2134 2494 3507 3752
Size: 627 186 261 513 314 360 1013 245
Cuts at: 3752 233
Size: 1190
Fragments arranged by size:
1190 1013 627 513 360 314 261 245
186
NaeI GCC'GGC
Cuts at: 343 379 2890 343
Size: 36 2511 2162
Fragments arranged by size:
2511 2162 36
NarI GG'CG_CC
Cuts at: 1366 2140 2388 1366
Size: 774 248 3687
Fragments arranged by size:
3687 774 248
NcoI C'CATG_G
Cuts at: 107 788 1098 107
Size: 681 310 3718
Fragments arranged by size:
3718 681 310
NgoMIV G'CCGG_C
Cuts at: 341 377 2888 341
Size: 36 2511 2162
Fragments arranged by size:
2511 2162 36
NheI G'CTAG_C
Cuts at: 1347 1347
Size: 4709
NspI r_CATG'y
Cuts at: 1203 1762 2262 3169 1203
Size: 559 500 907 2743
Fragments arranged by size:
2743 907 559 500
PasI CC'CwG_GG
Cuts at: 1270 1270
Size: 4709
PciI A'CATG_T
Cuts at: 3165 3165
Size: 4709
PflMI CCAn_nnn'nTGG
Cuts at: 555 1173 1270 555
Size: 618 97 3994
Fragments arranged by size:
3994 618 97
PfoI T'CCnGG_A
Cuts at: 254 1017 254
Size: 763 3946
PmeI GTTT'AAAC
Cuts at: 1708 1708
Size: 4709
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3848 3880 3848
Size: 32 4677
PpuMI rG'GwC_Cy
Cuts at: 505 505
Size: 4709
PspOMI G'GGCC_C
Cuts at: 689 1083 689
Size: 394 4315
PstI C_TGCA'G
Cuts at: 376 1756 2441 376
Size: 1380 685 2644
Fragments arranged by size:
2644 1380 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4709
PvuII CAG'CTG
Cuts at: 233 860 2134 2494 233
Size: 627 1274 360 2448
Fragments arranged by size:
2448 1274 627 360
RsrII CG'GwC_CG
Cuts at: 2904 2904
Size: 4709
SacI G_AGCT'C
Cuts at: 871 1723 871
Size: 852 3857
SalI G'TCGA_C
Cuts at: 1746 1746
Size: 4709
SanDI GG'GwC_CC
Cuts at: 505 505
Size: 4709
SapI GCTCTTCn'nnn_
Cuts at: 2732 2942 2732
Size: 210 4499
SbfI CC_TGCA'GG
Cuts at: 1756 1756
Size: 4709
ScaI AGT'ACT
Cuts at: 4226 4226
Size: 4709
SexAI A'CCwGG_T
Cuts at: 194 194
Size: 4709
SfcI C'TryA_G
Cuts at: 33 372 1752 2437 3430 3621 33
Size: 339 1380 685 993 191 1121
Fragments arranged by size:
1380 1121 993 685 339 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1309 1309
Size: 4709
SfoI GGC'GCC
Cuts at: 1367 2141 2389 1367
Size: 774 248 3687
Fragments arranged by size:
3687 774 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4709
SmaI CCC'GGG
Cuts at: 511 1206 1731 511
Size: 695 525 3489
Fragments arranged by size:
3489 695 525
SmlI C'TyrA_G
Cuts at: 391 1864 2779 3271 3533 391
Size: 1473 915 492 262 1567
Fragments arranged by size:
1567 1473 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4709
SphI G_CATG'C
Cuts at: 1203 1762 2262 1203
Size: 559 500 3650
Fragments arranged by size:
3650 559 500
StuI AGG'CCT
Cuts at: 737 769 737
Size: 32 4677
StyI C'CwwG_G
Cuts at: 107 346 656 761 788 1098 1241 1843 2197
Size: 239 310 105 27 310 143 602 354
Cuts at: 2197 107
Size: 2619
Fragments arranged by size:
2619 602 354 310 310 239 143 105
27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 542 1111 1112 1161 1165 1386 2292 2425 2884
Size: 569 1 49 4 221 906 133 459
Cuts at: 2884 3093 542
Size: 209 2158
Fragments arranged by size:
2158 906 569 459 221 209 133 49
4 1
TatI w'GTAC_w
Cuts at: 4224 4224
Size: 4709
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4226 4258 4226
Size: 32 4677
Tth111I GACn'n_nGTC
Cuts at: 1396 2506 1396
Size: 1110 3599
XbaI T'CTAG_A
Cuts at: 1740 1740
Size: 4709
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2204 2204
Size: 4709
XmaI C'CCGG_G
Cuts at: 509 1204 1729 509
Size: 695 525 3489
Fragments arranged by size:
3489 695 525
ZraI GAC'GTC
Cuts at: 1629 1629
Size: 4709
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI AscI AvrII BaeI
BbvCI BfrBI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI EcoRV FspAI HindIII HpaI MfeI
NdeI NotI NruI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SacII SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI