Restriction map for
pF1KB9746
(Circular) (Six-base) MAPSORT of: pF1KB9746.seq Check: 5938 from: 1 to: 4739
pF1KB9746 4739 bp
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AccI GT'mk_AC
Cuts at: 210 1777 210
Size: 1567 3172
Acc65I G'GTAC_C
Cuts at: 1755 1755
Size: 4739
AclI AA'CG_TT
Cuts at: 4579 4579
Size: 4739
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 654 990 1242 1410 2564 2996 3743 4011 654
Size: 336 252 168 1154 432 747 268 1382
Fragments arranged by size:
1382 1154 747 432 336 268 252 168
AflIII A'CryG_T
Cuts at: 2091 3195 4172 2091
Size: 1104 977 2658
Fragments arranged by size:
2658 1104 977
AgeI A'CCGG_T
Cuts at: 3127 3127
Size: 4739
AhdI GACnn_n'nnGTC
Cuts at: 251 1343 251
Size: 1092 3647
AleI CACnn'nnGTG
Cuts at: 461 629 1553 1637 461
Size: 168 924 84 3563
Fragments arranged by size:
3563 924 168 84
AlwNI CAG_nnn'CTG
Cuts at: 2044 3611 2044
Size: 1567 3172
ApaLI G'TGCA_C
Cuts at: 1264 3509 1264
Size: 2245 2494
ApoI r'AATT_y
Cuts at: 452 704 788 956 1292 1460 1743 4092 4348
Size: 252 84 168 336 168 283 2349 256
Cuts at: 4348 452
Size: 843
Fragments arranged by size:
2349 843 336 283 256 252 168 168
84
AseI AT'TA_AT
Cuts at: 20 20
Size: 4739
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4739
AvaI C'yCGr_G
Cuts at: 1759 1759
Size: 4739
BamHI G'GATC_C
Cuts at: 1124 1764 1124
Size: 640 4099
BanI G'GyrC_C
Cuts at: 1755 2169 2417 2452 1755
Size: 414 248 35 4042
Fragments arranged by size:
4042 414 248 35
BanII G_rGCy'C
Cuts at: 1753 2783 1753
Size: 1030 3709
BbeI G_GCGC'C
Cuts at: 2173 2421 2173
Size: 248 4491
BbsI GAAGACnn'nnnn_
Cuts at: 1044 1678 1044
Size: 634 4105
BciVI GTATCCnnnnn_n'
Cuts at: 2637 3398 4739 2637
Size: 761 1341 2637
Fragments arranged by size:
2637 1341 761
BclI T'GATC_A
Cuts at: 2259 2259
Size: 4739
BglII A'GATC_T
Cuts at: 2254 4291 2254
Size: 2037 2702
BlpI GC'TnA_GC
Cuts at: 429 776 1851 429
Size: 347 1075 3317
Fragments arranged by size:
3317 1075 347
Bme1580I G_kGCm'C
Cuts at: 1268 2364 2457 3513 1268
Size: 1096 93 1056 2494
Fragments arranged by size:
2494 1096 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 554 1142 1478 1951 2032 2367 4511 554
Size: 588 336 473 81 335 2144 782
Fragments arranged by size:
2144 782 588 473 336 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3106 3106
Size: 4739
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1238 1238
Size: 4739
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1915 2794 3286 3584 1915
Size: 879 492 298 3070
Fragments arranged by size:
3070 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4443 4443
Size: 4739
BsaAI yAC'GTr
Cuts at: 223 1269 2092 223
Size: 1046 823 2870
Fragments arranged by size:
2870 1046 823
BsaBI GATnn'nnATC
Cuts at: 6 2253 4290 6
Size: 2247 2037 455
Fragments arranged by size:
2247 2037 455
BsaHI Gr'CG_yC
Cuts at: 2170 2418 3120 4616 2170
Size: 248 702 1496 2293
Fragments arranged by size:
2293 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1928 2449 3127 3401 3548 3959 1928
Size: 521 678 274 147 411 2708
Fragments arranged by size:
2708 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 667 667
Size: 4739
BseYI C'CCAG_C
Cuts at: 2164 2922 3499 3995 2164
Size: 758 577 496 2908
Fragments arranged by size:
2908 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2281 2327 3535 104
Size: 2177 46 1208 1308
Fragments arranged by size:
2177 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1268 1753 2531 2721 3513 1268
Size: 485 778 190 792 2494
Fragments arranged by size:
2494 792 778 485 190
BsmI GAATG_Cn'
Cuts at: 736 997 736
Size: 261 4478
Bsp1286I G_dGCh'C
Cuts at: 1268 1753 2364 2457 2531 2721 2783 3513 1268
Size: 485 611 93 74 190 62 730 2494
Fragments arranged by size:
2494 730 611 485 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3959 3959
Size: 4739
BspHI T'CATG_A
Cuts at: 3915 3915
Size: 4739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1789 2003 2305 2686 1789
Size: 214 302 381 3842
Fragments arranged by size:
3842 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3031 3085 4569 4734 50
Size: 2981 54 1484 165 55
Fragments arranged by size:
2981 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 493 2651 493
Size: 2158 2581
BsrFI r'CCGG_y
Cuts at: 2737 2918 3127 2737
Size: 181 209 4349
Fragments arranged by size:
4349 209 181
BsrGI T'GTAC_A
Cuts at: 1362 1362
Size: 4739
BssSI C'ACGA_G
Cuts at: 3010 3368 3010
Size: 358 4381
BstAPI GCAn_nnn'nTGC
Cuts at: 992 2116 992
Size: 1124 3615
BstBI TT'CG_AA
Cuts at: 3100 3100
Size: 4739
BstYI r'GATC_y
Cuts at: 1 1124 1764 2231 2254 2589 2835 3847 3933
Size: 1123 640 467 23 335 246 1012 86
Cuts at: 3933 3945 4291 1
Size: 12 346 449
Fragments arranged by size:
1123 1012 640 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4739
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2689 2870 3033 2689
Size: 181 163 4395
Fragments arranged by size:
4395 181 163
BtsI GCAGTG_nn'
Cuts at: 1982 1982
Size: 4739
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 723 758 1647 1682 723
Size: 35 889 35 3780
Fragments arranged by size:
3780 889 35 35
DraI TTT'AAA
Cuts at: 1653 1738 1653
Size: 85 4654
DrdI GACnn_nn'nnGTC
Cuts at: 2445 3303 2445
Size: 858 3881
EaeI y'GGCC_r
Cuts at: 2324 2498 2889 2916 4261 2324
Size: 174 391 27 1345 2802
Fragments arranged by size:
2802 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2324 2324
Size: 4739
EarI CTCTTCn'nnn_
Cuts at: 2762 2972 2762
Size: 210 4529
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2947 3257 3403 4549 2947
Size: 310 146 1146 3137
Fragments arranged by size:
3137 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1751 1751
Size: 4739
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 654 990 1238 1242 1410 2564 2996 3743 4011
Size: 336 248 4 168 1154 432 747 268
Cuts at: 4011 654
Size: 1382
Fragments arranged by size:
1382 1154 747 432 336 268 248 168
4
EcoO109I rG'GnC_Cy
Cuts at: 1878 1878
Size: 4739
EcoRI G'AATT_C
Cuts at: 452 704 788 956 1460 1743 452
Size: 252 84 168 504 283 3448
Fragments arranged by size:
3448 504 283 252 168 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2758 2758
Size: 4739
FspI TGC'GCA
Cuts at: 2520 2520
Size: 4739
HaeII r_GCGC'y
Cuts at: 2173 2421 3443 4422 2173
Size: 248 1022 979 2490
Fragments arranged by size:
2490 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 237 269 335 367 1329 1361 2581 2611 2613
Size: 32 66 32 962 32 1220 30 2
Cuts at: 2613 2643 237
Size: 30 2333
Fragments arranged by size:
2333 1220 962 66 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1778 1778
Size: 4739
HindIII A'AGCT_T
Cuts at: 581 601 581
Size: 20 4719
KasI G'GCGC_C
Cuts at: 2169 2417 2169
Size: 248 4491
KpnI G_GTAC'C
Cuts at: 1759 1759
Size: 4739
MluI A'CGCG_T
Cuts at: 4172 4172
Size: 4739
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 110 3410 3594 110
Size: 3300 184 1255
Fragments arranged by size:
3300 1255 184
MscI TGG'CCA
Cuts at: 2500 2500
Size: 4739
MslI CAynn'nnrTG
Cuts at: 225 293 377 461 477 561 629 713 881
Size: 68 84 84 16 84 68 84 168
Cuts at: 881 1301 1541 1553 1637 1721 2728 2855 225
Size: 420 240 12 84 84 1007 127 2109
Fragments arranged by size:
2109 1007 420 240 168 127 84 84
84 84 84 84 68 68 16 12
MspA1I CmG'CkG
Cuts at: 1626 1850 2164 2524 3537 3782 1626
Size: 224 314 360 1013 245 2583
Fragments arranged by size:
2583 1013 360 314 245 224
NaeI GCC'GGC
Cuts at: 2920 2920
Size: 4739
NarI GG'CG_CC
Cuts at: 2170 2418 2170
Size: 248 4491
NcoI C'CATG_G
Cuts at: 107 107
Size: 4739
NdeI CA'TA_TG
Cuts at: 558 1718 558
Size: 1160 3579
NgoMIV G'CCGG_C
Cuts at: 2918 2918
Size: 4739
NspI r_CATG'y
Cuts at: 376 1792 2292 3199 376
Size: 1416 500 907 1916
Fragments arranged by size:
1916 1416 907 500
PciI A'CATG_T
Cuts at: 3195 3195
Size: 4739
PmeI GTTT'AAAC
Cuts at: 1738 1738
Size: 4739
PmlI CAC'GTG
Cuts at: 223 223
Size: 4739
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3878 3910 3878
Size: 32 4707
PstI C_TGCA'G
Cuts at: 1786 2471 1786
Size: 685 4054
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4739
PvuII CAG'CTG
Cuts at: 2164 2524 2164
Size: 360 4379
RsrII CG'GwC_CG
Cuts at: 2934 2934
Size: 4739
SacI G_AGCT'C
Cuts at: 1753 1753
Size: 4739
SalI G'TCGA_C
Cuts at: 1776 1776
Size: 4739
SapI GCTCTTCn'nnn_
Cuts at: 2762 2972 2762
Size: 210 4529
SbfI CC_TGCA'GG
Cuts at: 1786 1786
Size: 4739
ScaI AGT'ACT
Cuts at: 621 1095 4256 621
Size: 474 3161 1104
Fragments arranged by size:
3161 1104 474
SexAI A'CCwGG_T
Cuts at: 686 686
Size: 4739
SfcI C'TryA_G
Cuts at: 33 1782 2467 3460 3651 33
Size: 1749 685 993 191 1121
Fragments arranged by size:
1749 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2171 2419 2171
Size: 248 4491
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4739
SmaI CCC'GGG
Cuts at: 1761 1761
Size: 4739
SmlI C'TyrA_G
Cuts at: 1894 2809 3301 3563 1894
Size: 915 492 262 3070
Fragments arranged by size:
3070 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4739
SphI G_CATG'C
Cuts at: 1792 2292 1792
Size: 500 4239
StuI AGG'CCT
Cuts at: 415 667 751 919 1087 1255 1423 1591 415
Size: 252 84 168 168 168 168 168 3563
Fragments arranged by size:
3563 252 168 168 168 168 168 84
StyI C'CwwG_G
Cuts at: 107 1873 2227 107
Size: 1766 354 2619
Fragments arranged by size:
2619 1766 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 635 2322 2455 2914 3123 635
Size: 1687 133 459 209 2251
Fragments arranged by size:
2251 1687 459 209 133
TatI w'GTAC_w
Cuts at: 525 619 1093 1362 4254 525
Size: 94 474 269 2892 1010
Fragments arranged by size:
2892 1010 474 269 94
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4256 4288 4256
Size: 32 4707
Tth111I GACn'n_nGTC
Cuts at: 2536 2536
Size: 4739
XbaI T'CTAG_A
Cuts at: 1770 1770
Size: 4739
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2234 2234
Size: 4739
XmaI C'CCGG_G
Cuts at: 1759 1759
Size: 4739
XmnI GAAnn'nnTTC
Cuts at: 667 835 1003 667
Size: 168 168 4403
Fragments arranged by size:
4403 168 168
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PmeI PmlI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI