Restriction map for
pF1KB9747
(Circular) (Six-base) MAPSORT of: pF1KB9747.seq Check: 5897 from: 1 to: 4748
pF1KB9747 4748 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AatII G_ACGT'C
Cuts at: 269 269
Size: 4748
AccI GT'mk_AC
Cuts at: 1526 1786 1526
Size: 260 4488
Acc65I G'GTAC_C
Cuts at: 1132 1764 1132
Size: 632 4116
AclI AA'CG_TT
Cuts at: 4588 4588
Size: 4748
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 1355 2573 3005 3752 4020 640
Size: 715 1218 432 747 268 1368
Fragments arranged by size:
1368 1218 747 715 432 268
AfeI AGC'GCT
Cuts at: 167 167
Size: 4748
AflIII A'CryG_T
Cuts at: 758 1361 2100 3204 4181 758
Size: 603 739 1104 977 1325
Fragments arranged by size:
1325 1104 977 739 603
AgeI A'CCGG_T
Cuts at: 3136 3136
Size: 4748
AleI CACnn'nnGTG
Cuts at: 757 1520 757
Size: 763 3985
AlwNI CAG_nnn'CTG
Cuts at: 618 1062 2053 3620 618
Size: 444 991 1567 1746
Fragments arranged by size:
1746 1567 991 444
ApaI G_GGCC'C
Cuts at: 156 163 982 1304 1568 156
Size: 7 819 322 264 3336
Fragments arranged by size:
3336 819 322 264 7
ApaLI G'TGCA_C
Cuts at: 3518 3518
Size: 4748
ApoI r'AATT_y
Cuts at: 1752 4101 4357 1752
Size: 2349 256 2143
Fragments arranged by size:
2349 2143 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4748
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4748
AvaI C'yCGr_G
Cuts at: 156 196 249 256 1303 1682 1768 156
Size: 40 53 7 1047 379 86 3136
Fragments arranged by size:
3136 1047 379 86 53 40 7
BamHI G'GATC_C
Cuts at: 1773 1773
Size: 4748
BanI G'GyrC_C
Cuts at: 358 991 1132 1764 2178 2426 2461 358
Size: 633 141 632 414 248 35 2645
Fragments arranged by size:
2645 633 632 414 248 141 35
BanII G_rGCy'C
Cuts at: 156 163 203 335 585 982 1246 1304 1495
Size: 7 40 132 250 397 264 58 191
Cuts at: 1495 1568 1762 2792 156
Size: 73 194 1030 2112
Fragments arranged by size:
2112 1030 397 264 250 194 191 132
73 58 40 7
BbeI G_GCGC'C
Cuts at: 2182 2430 2182
Size: 248 4500
BbvCI CC'TCA_GC
Cuts at: 223 1069 223
Size: 846 3902
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4714
BciVI GTATCCnnnnn_n'
Cuts at: 2646 3407 4748 2646
Size: 761 1341 2646
Fragments arranged by size:
2646 1341 761
BclI T'GATC_A
Cuts at: 368 2268 368
Size: 1900 2848
BglI GCCn_nnn'nGGC
Cuts at: 151 546 151
Size: 395 4353
BglII A'GATC_T
Cuts at: 2263 4300 2263
Size: 2037 2711
BlpI GC'TnA_GC
Cuts at: 527 1240 1351 1860 527
Size: 713 111 509 3415
Fragments arranged by size:
3415 713 509 111
Bme1580I G_kGCm'C
Cuts at: 156 163 361 982 1035 1304 1568 2373 2466
Size: 7 198 621 53 269 264 805 93
Cuts at: 2466 3522 156
Size: 1056 1382
Fragments arranged by size:
1382 1056 805 621 269 264 198 93
53 7
BmgBI CAC'GTC
Cuts at: 975 975
Size: 4748
BmrI ACTGGGnnnn_n'
Cuts at: 1536 1960 2041 2376 4520 1536
Size: 424 81 335 2144 1764
Fragments arranged by size:
2144 1764 424 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3115 3115
Size: 4748
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 991 991
Size: 4748
Bpu10I CC'TnA_GC
Cuts at: 223 1069 223
Size: 846 3902
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1080 1924 2803 3295 3593 1080
Size: 844 879 492 298 2235
Fragments arranged by size:
2235 879 844 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 609 763 4452 609
Size: 154 3689 905
Fragments arranged by size:
3689 905 154
BsaAI yAC'GTr
Cuts at: 759 2101 759
Size: 1342 3406
BsaBI GATnn'nnATC
Cuts at: 6 370 479 2262 4299 6
Size: 364 109 1783 2037 455
Fragments arranged by size:
2037 1783 455 364 109
BsaHI Gr'CG_yC
Cuts at: 266 2179 2427 3129 4625 266
Size: 1913 248 702 1496 389
Fragments arranged by size:
1913 1496 702 389 248
BsaWI w'CCGG_w
Cuts at: 969 1937 2458 3136 3410 3557 3968 969
Size: 968 521 678 274 147 411 1749
Fragments arranged by size:
1749 968 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 154
Size: 4748
BseYI C'CCAG_C
Cuts at: 162 400 815 953 1496 1567 2173 2931 3508
Size: 238 415 138 543 71 606 758 577
Cuts at: 3508 4004 162
Size: 496 906
Fragments arranged by size:
906 758 606 577 543 496 415 238
138 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 735 1420 735
Size: 685 4063
BsiEI CG_ry'CG
Cuts at: 104 2290 2336 3544 104
Size: 2186 46 1208 1308
Fragments arranged by size:
2186 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 335 1495 1762 2540 2730 3522 335
Size: 1160 267 778 190 792 1561
Fragments arranged by size:
1561 1160 792 778 267 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1121 1121
Size: 4748
Bsp1286I G_dGCh'C
Cuts at: 156 163 203 335 361 585 982 1035 1246
Size: 7 40 132 26 224 397 53 211
Cuts at: 1246 1304 1495 1568 1762 2373 2466 2540 2730
Size: 58 191 73 194 611 93 74 190
Cuts at: 2730 2792 3522 156
Size: 62 730 1382
Fragments arranged by size:
1382 730 611 397 224 211 194 191
190 132 93 74 73 62 58 53
40 26 7
BspEI T'CCGG_A
Cuts at: 3968 3968
Size: 4748
BspHI T'CATG_A
Cuts at: 929 3924 929
Size: 2995 1753
BspMI ACCTGCnnnn'nnnn_
Cuts at: 884 1070 1343 1798 2012 2314 2695 884
Size: 186 273 455 214 302 381 2937
Fragments arranged by size:
2937 455 381 302 273 214 186
BsrBI CCG'CTC
Cuts at: 50 1486 1560 3040 3094 4578 4743 50
Size: 1436 74 1480 54 1484 165 55
Fragments arranged by size:
1484 1480 1436 165 74 55 54
BsrDI GCAATG_nn'
Cuts at: 2660 2660
Size: 4748
BsrFI r'CCGG_y
Cuts at: 2746 2927 3136 2746
Size: 181 209 4358
Fragments arranged by size:
4358 209 181
BssSI C'ACGA_G
Cuts at: 349 1021 3019 3377 349
Size: 672 1998 358 1720
Fragments arranged by size:
1998 1720 672 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1062 2125 1062
Size: 1063 3685
BstBI TT'CG_AA
Cuts at: 3109 3109
Size: 4748
BstEII G'GTnAC_C
Cuts at: 1263 1263
Size: 4748
BstYI r'GATC_y
Cuts at: 1 1773 2240 2263 2598 2844 3856 3942 3954
Size: 1772 467 23 335 246 1012 86 12
Cuts at: 3954 4300 1
Size: 346 449
Fragments arranged by size:
1772 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 284 688 1465 284
Size: 404 777 3567
Fragments arranged by size:
3567 777 404
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 466 2698 2879 3042 466
Size: 2232 181 163 2172
Fragments arranged by size:
2232 2172 181 163
BtsI GCAGTG_nn'
Cuts at: 740 867 1110 1263 1991 740
Size: 127 243 153 728 3497
Fragments arranged by size:
3497 728 243 153 127
DraI TTT'AAA
Cuts at: 1747 1747
Size: 4748
DrdI GACnn_nn'nnGTC
Cuts at: 1374 2454 3312 1374
Size: 1080 858 2810
Fragments arranged by size:
2810 1080 858
EaeI y'GGCC_r
Cuts at: 651 2333 2507 2898 2925 4270 651
Size: 1682 174 391 27 1345 1129
Fragments arranged by size:
1682 1345 1129 391 174 27
EagI C'GGCC_G
Cuts at: 2333 2333
Size: 4748
EarI CTCTTCn'nnn_
Cuts at: 1108 1188 2771 2981 1108
Size: 80 1583 210 2875
Fragments arranged by size:
2875 1583 210 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2956 3266 3412 4558 2956
Size: 310 146 1146 3146
Fragments arranged by size:
3146 1146 310 146
EcoICRI GAG'CTC
Cuts at: 333 1493 1760 333
Size: 1160 267 3321
Fragments arranged by size:
3321 1160 267
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 991 1355 2573 3005 3752 4020 640
Size: 351 364 1218 432 747 268 1368
Fragments arranged by size:
1368 1218 747 432 364 351 268
EcoNI CCTnn'n_nnAGG
Cuts at: 575 575
Size: 4748
EcoO109I rG'GnC_Cy
Cuts at: 123 153 253 979 1059 1300 1564 1887 123
Size: 30 100 726 80 241 264 323 2984
Fragments arranged by size:
2984 726 323 264 241 100 80 30
EcoRI G'AATT_C
Cuts at: 1752 1752
Size: 4748
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2767 2767
Size: 4748
FspI TGC'GCA
Cuts at: 2529 2529
Size: 4748
HaeII r_GCGC'y
Cuts at: 169 217 1574 2182 2430 3452 4431 169
Size: 48 1357 608 248 1022 979 486
Fragments arranged by size:
1357 1022 979 608 486 248 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2590 2620 2622 2652 2590
Size: 30 2 30 4686
Fragments arranged by size:
4686 30 30 2
HincII GTy'rAC
Cuts at: 1787 1787
Size: 4748
KasI G'GCGC_C
Cuts at: 2178 2426 2178
Size: 248 4500
KpnI G_GTAC'C
Cuts at: 1136 1768 1136
Size: 632 4116
MluI A'CGCG_T
Cuts at: 4181 4181
Size: 4748
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3419 3603 3419
Size: 184 4564
MscI TGG'CCA
Cuts at: 653 2509 653
Size: 1856 2892
MslI CAynn'nnrTG
Cuts at: 310 364 424 757 784 1456 1459 1520 1637
Size: 54 60 333 27 672 3 61 117
Cuts at: 1637 2737 2864 310
Size: 1100 127 2194
Fragments arranged by size:
2194 1100 672 333 127 117 61 60
54 27 3
MspA1I CmG'CkG
Cuts at: 227 286 600 690 819 1350 1467 1859 2173
Size: 59 314 90 129 531 117 392 314
Cuts at: 2173 2533 3546 3791 227
Size: 360 1013 245 1184
Fragments arranged by size:
1184 1013 531 392 360 314 314 245
129 117 90 59
NaeI GCC'GGC
Cuts at: 2929 2929
Size: 4748
NarI GG'CG_CC
Cuts at: 2179 2427 2179
Size: 248 4500
NgoMIV G'CCGG_C
Cuts at: 2927 2927
Size: 4748
NspI r_CATG'y
Cuts at: 1283 1365 1801 2301 3208 1283
Size: 82 436 500 907 2823
Fragments arranged by size:
2823 907 500 436 82
PciI A'CATG_T
Cuts at: 1361 3204 1361
Size: 1843 2905
PmeI GTTT'AAAC
Cuts at: 1747 1747
Size: 4748
PmlI CAC'GTG
Cuts at: 759 759
Size: 4748
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3887 3919 3887
Size: 32 4716
PpuMI rG'GwC_Cy
Cuts at: 253 1059 253
Size: 806 3942
PspOMI G'GGCC_C
Cuts at: 152 159 978 1300 1564 152
Size: 7 819 322 264 3336
Fragments arranged by size:
3336 819 322 264 7
PstI C_TGCA'G
Cuts at: 116 896 1258 1795 2480 116
Size: 780 362 537 685 2384
Fragments arranged by size:
2384 780 685 537 362
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4748
PvuII CAG'CTG
Cuts at: 600 819 2173 2533 600
Size: 219 1354 360 2815
Fragments arranged by size:
2815 1354 360 219
RsrII CG'GwC_CG
Cuts at: 2943 2943
Size: 4748
SacI G_AGCT'C
Cuts at: 335 1495 1762 335
Size: 1160 267 3321
Fragments arranged by size:
3321 1160 267
SacII CC_GC'GG
Cuts at: 287 691 1468 287
Size: 404 777 3567
Fragments arranged by size:
3567 777 404
SalI G'TCGA_C
Cuts at: 1785 1785
Size: 4748
SanDI GG'GwC_CC
Cuts at: 253 253
Size: 4748
SapI GCTCTTCn'nnn_
Cuts at: 2771 2981 2771
Size: 210 4538
SbfI CC_TGCA'GG
Cuts at: 1795 1795
Size: 4748
ScaI AGT'ACT
Cuts at: 4265 4265
Size: 4748
SfcI C'TryA_G
Cuts at: 33 112 892 1254 1392 1791 2476 3469 3660
Size: 79 780 362 138 399 685 993 191
Cuts at: 3660 33
Size: 1121
Fragments arranged by size:
1121 993 780 685 399 362 191 138
79
SfiI GGCCn_nnn'nGGCC
Cuts at: 151 151
Size: 4748
SfoI GGC'GCC
Cuts at: 2180 2428 2180
Size: 248 4500
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4748
SmaI CCC'GGG
Cuts at: 158 251 258 1770 158
Size: 93 7 1512 3136
Fragments arranged by size:
3136 1512 93 7
SmlI C'TyrA_G
Cuts at: 1095 1903 2818 3310 3572 1095
Size: 808 915 492 262 2271
Fragments arranged by size:
2271 915 808 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4748
SphI G_CATG'C
Cuts at: 1801 2301 1801
Size: 500 4248
StuI AGG'CCT
Cuts at: 145 145
Size: 4748
StyI C'CwwG_G
Cuts at: 845 1602 1882 2236 845
Size: 757 280 354 3357
Fragments arranged by size:
3357 757 354 280
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 769 829 1009 1099 2331 2464 2923 3132 769
Size: 60 180 90 1232 133 459 209 2385
Fragments arranged by size:
2385 1232 459 209 180 133 90 60
TatI w'GTAC_w
Cuts at: 1286 4263 1286
Size: 2977 1771
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4265 4297 4265
Size: 32 4716
Tth111I GACn'n_nGTC
Cuts at: 268 463 2545 268
Size: 195 2082 2471
Fragments arranged by size:
2471 2082 195
XbaI T'CTAG_A
Cuts at: 1779 1779
Size: 4748
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 972 2243 972
Size: 1271 3477
XmaI C'CCGG_G
Cuts at: 156 249 256 1768 156
Size: 93 7 1512 3136
Fragments arranged by size:
3136 1512 93 7
XmnI GAAnn'nnTTC
Cuts at: 343 343
Size: 4748
ZraI GAC'GTC
Cuts at: 267 267
Size: 4748
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AlfI AloI AscI AvrII BaeI
BbsI BfrBI BmtI BsaXI BsiWI BsmI BsrGI BssHII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PshAI PsiI
PspXI PsrI SexAI SgrAI SnaBI SrfI SspI SwaI
XhoI