Restriction map for
pF1KB9749
(Circular) (Six-base) MAPSORT of: pF1KB9749.seq Check: 3202 from: 1 to: 4790
pF1KB9749 4790 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 464 464
Size: 4790
AccI GT'mk_AC
Cuts at: 1828 1828
Size: 4790
Acc65I G'GTAC_C
Cuts at: 1806 1806
Size: 4790
AclI AA'CG_TT
Cuts at: 4630 4630
Size: 4790
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1407 2615 3047 3794 4062 1407
Size: 1208 432 747 268 2135
Fragments arranged by size:
2135 1208 747 432 268
AflIII A'CryG_T
Cuts at: 297 2142 3246 4223 297
Size: 1845 1104 977 864
Fragments arranged by size:
1845 1104 977 864
AgeI A'CCGG_T
Cuts at: 3178 3178
Size: 4790
AhdI GACnn_n'nnGTC
Cuts at: 558 1335 1365 558
Size: 777 30 3983
Fragments arranged by size:
3983 777 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1666 1666
Size: 4790
AlwNI CAG_nnn'CTG
Cuts at: 2095 3662 2095
Size: 1567 3223
ApaI G_GGCC'C
Cuts at: 923 923
Size: 4790
ApaLI G'TGCA_C
Cuts at: 120 367 3560 120
Size: 247 3193 1350
Fragments arranged by size:
3193 1350 247
ApoI r'AATT_y
Cuts at: 1794 4143 4399 1794
Size: 2349 256 2185
Fragments arranged by size:
2349 2185 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4790
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4790
AvaI C'yCGr_G
Cuts at: 223 947 1148 1398 1534 1810 223
Size: 724 201 250 136 276 3203
Fragments arranged by size:
3203 724 276 250 201 136
BamHI G'GATC_C
Cuts at: 1815 1815
Size: 4790
BanI G'GyrC_C
Cuts at: 837 1045 1573 1806 2220 2468 2503 837
Size: 208 528 233 414 248 35 3124
Fragments arranged by size:
3124 528 414 248 233 208 35
BanII G_rGCy'C
Cuts at: 314 748 923 1148 1478 1534 1804 2834 314
Size: 434 175 225 330 56 270 1030 2270
Fragments arranged by size:
2270 1030 434 330 270 225 175 56
BbeI G_GCGC'C
Cuts at: 841 2224 2472 841
Size: 1383 248 3159
Fragments arranged by size:
3159 1383 248
BbsI GAAGACnn'nnnn_
Cuts at: 255 634 255
Size: 379 4411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1363 1397 1363
Size: 34 4756
BciVI GTATCCnnnnn_n'
Cuts at: 2688 3449 4790 2688
Size: 761 1341 2688
Fragments arranged by size:
2688 1341 761
BclI T'GATC_A
Cuts at: 2310 2310
Size: 4790
BglI GCCn_nnn'nGGC
Cuts at: 1162 1162
Size: 4790
BglII A'GATC_T
Cuts at: 2305 4342 2305
Size: 2037 2753
BlpI GC'TnA_GC
Cuts at: 1902 1902
Size: 4790
Bme1580I G_kGCm'C
Cuts at: 124 371 923 1576 2415 2508 3564 124
Size: 247 552 653 839 93 1056 1350
Fragments arranged by size:
1350 1056 839 653 552 247 93
BmgBI CAC'GTC
Cuts at: 905 905
Size: 4790
BmrI ACTGGGnnnn_n'
Cuts at: 563 1218 2002 2083 2418 4562 563
Size: 655 784 81 335 2144 791
Fragments arranged by size:
2144 791 784 655 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1203 1377 3157 1203
Size: 174 1780 2836
Fragments arranged by size:
2836 1780 174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 1025 1189 1227 1233 1245 1395 1789 781
Size: 244 164 38 6 12 150 394 3782
Fragments arranged by size:
3782 394 244 164 150 38 12 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1549 1966 2845 3337 3635 1549
Size: 417 879 492 298 2704
Fragments arranged by size:
2704 879 492 417 298
BsaI GGTCTCn'nnnn_
Cuts at: 880 1732 4494 880
Size: 852 2762 1176
Fragments arranged by size:
2762 1176 852
BsaAI yAC'GTr
Cuts at: 2143 2143
Size: 4790
BsaBI GATnn'nnATC
Cuts at: 6 2304 4341 6
Size: 2298 2037 455
Fragments arranged by size:
2298 2037 455
BsaHI Gr'CG_yC
Cuts at: 656 838 2221 2469 3171 4667 656
Size: 182 1383 248 702 1496 779
Fragments arranged by size:
1496 1383 779 702 248 182
BsaWI w'CCGG_w
Cuts at: 500 1616 1979 2500 3178 3452 3599 4010 500
Size: 1116 363 521 678 274 147 411 1280
Fragments arranged by size:
1280 1116 678 521 411 363 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 322 352 487 517 1186 1216 1231 1261 1711
Size: 30 135 30 669 30 15 30 450
Cuts at: 1711 1741 322
Size: 30 3371
Fragments arranged by size:
3371 669 450 135 30 30 30 30
30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 343 400 738 844 1053 1056 1059 1062 1192
Size: 57 338 106 209 3 3 3 130
Cuts at: 1192 1357 1390 1446 1542 1690 343
Size: 165 33 56 96 148 3443
Fragments arranged by size:
3443 338 209 165 148 130 106 96
57 56 33 3 3 3
BseYI C'CCAG_C
Cuts at: 144 804 991 1465 2215 2973 3550 4046 144
Size: 660 187 474 750 758 577 496 888
Fragments arranged by size:
888 758 750 660 577 496 474 187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 256
Size: 4790
BsiEI CG_ry'CG
Cuts at: 104 967 2332 2378 3586 104
Size: 863 1365 46 1208 1308
Fragments arranged by size:
1365 1308 1208 863 46
BsiHKAI G_wGCw'C
Cuts at: 124 371 710 748 1041 1804 2582 2772 3564
Size: 247 339 38 293 763 778 190 792
Cuts at: 3564 124
Size: 1350
Fragments arranged by size:
1350 792 778 763 339 293 247 190
38
Bsp1286I G_dGCh'C
Cuts at: 124 314 371 710 748 923 1041 1148 1478
Size: 190 57 339 38 175 118 107 330
Cuts at: 1478 1534 1576 1804 2415 2508 2582 2772 2834
Size: 56 42 228 611 93 74 190 62
Cuts at: 2834 3564 124
Size: 730 1350
Fragments arranged by size:
1350 730 611 339 330 228 190 190
175 118 107 93 74 62 57 56
42 38
BspEI T'CCGG_A
Cuts at: 4010 4010
Size: 4790
BspHI T'CATG_A
Cuts at: 3966 3966
Size: 4790
BspMI ACCTGCnnnn'nnnn_
Cuts at: 464 1340 1840 2054 2356 2737 464
Size: 876 500 214 302 381 2517
Fragments arranged by size:
2517 876 500 381 302 214
BsrBI CCG'CTC
Cuts at: 50 717 3082 3136 4620 4785 50
Size: 667 2365 54 1484 165 55
Fragments arranged by size:
2365 1484 667 165 55 54
BsrDI GCAATG_nn'
Cuts at: 235 2702 235
Size: 2467 2323
BsrFI r'CCGG_y
Cuts at: 520 815 966 1092 1384 2788 2969 3178 520
Size: 295 151 126 292 1404 181 209 2132
Fragments arranged by size:
2132 1404 295 292 209 181 151 126
BssSI C'ACGA_G
Cuts at: 3061 3419 3061
Size: 358 4432
BstAPI GCAn_nnn'nTGC
Cuts at: 2167 2167
Size: 4790
BstBI TT'CG_AA
Cuts at: 3151 3151
Size: 4790
BstEII G'GTnAC_C
Cuts at: 696 696
Size: 4790
BstXI CCAn_nnnn'nTGG
Cuts at: 759 759
Size: 4790
BstYI r'GATC_y
Cuts at: 1 1559 1815 2282 2305 2640 2886 3898 3984
Size: 1558 256 467 23 335 246 1012 86
Cuts at: 3984 3996 4342 1
Size: 12 346 449
Fragments arranged by size:
1558 1012 467 449 346 335 256 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 179 216 491 642 1319 1775 107
Size: 72 37 275 151 677 456 3122
Fragments arranged by size:
3122 677 456 275 151 72 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 136 2740 2921 3084 136
Size: 2604 181 163 1842
Fragments arranged by size:
2604 1842 181 163
BtsI GCAGTG_nn'
Cuts at: 2033 2033
Size: 4790
DraI TTT'AAA
Cuts at: 1789 1789
Size: 4790
DraIII CAC_nnn'GTG
Cuts at: 249 696 249
Size: 447 4343
DrdI GACnn_nn'nnGTC
Cuts at: 2496 3354 2496
Size: 858 3932
EaeI y'GGCC_r
Cuts at: 964 968 1094 1683 1778 2375 2549 2940 2967
Size: 4 126 589 95 597 174 391 27
Cuts at: 2967 4312 964
Size: 1345 1442
Fragments arranged by size:
1442 1345 597 589 391 174 126 95
27 4
EagI C'GGCC_G
Cuts at: 964 2375 964
Size: 1411 3379
EarI CTCTTCn'nnn_
Cuts at: 1006 1368 1729 2813 3023 1006
Size: 362 361 1084 210 2773
Fragments arranged by size:
2773 1084 362 361 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2998 3308 3454 4600 2998
Size: 310 146 1146 3188
Fragments arranged by size:
3188 1146 310 146
EcoICRI GAG'CTC
Cuts at: 746 1802 746
Size: 1056 3734
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 1025 1189 1227 1233 1245 1395 1407 1789
Size: 244 164 38 6 12 150 12 382
Cuts at: 1789 2615 3047 3794 4062 781
Size: 826 432 747 268 1509
Fragments arranged by size:
1509 826 747 432 382 268 244 164
150 38 12 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1567 1567
Size: 4790
EcoO109I rG'GnC_Cy
Cuts at: 920 1028 1164 1306 1329 1929 920
Size: 108 136 142 23 600 3781
Fragments arranged by size:
3781 600 142 136 108 23
EcoRI G'AATT_C
Cuts at: 1794 1794
Size: 4790
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 279 420 492 873 2809 279
Size: 141 72 381 1936 2260
Fragments arranged by size:
2260 1936 381 141 72
FseI GG_CCGG'CC
Cuts at: 970 970
Size: 4790
FspI TGC'GCA
Cuts at: 166 1750 2571 166
Size: 1584 821 2385
Fragments arranged by size:
2385 1584 821
HaeII r_GCGC'y
Cuts at: 841 998 1451 1633 2224 2472 3494 4473 841
Size: 157 453 182 591 248 1022 979 1158
Fragments arranged by size:
1158 1022 979 591 453 248 182 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 544 576 1186 1218 1249 1281 1321 1351 1353
Size: 32 610 32 31 32 40 30 2
Cuts at: 1353 1383 1650 1682 1711 1743 2632 2662 2664
Size: 30 267 32 29 32 889 30 2
Cuts at: 2664 2694 544
Size: 30 2640
Fragments arranged by size:
2640 889 610 267 40 32 32 32
32 32 31 30 30 30 30 29
2 2
HincII GTy'rAC
Cuts at: 1829 1829
Size: 4790
KasI G'GCGC_C
Cuts at: 837 2220 2468 837
Size: 1383 248 3159
Fragments arranged by size:
3159 1383 248
KpnI G_GTAC'C
Cuts at: 1810 1810
Size: 4790
MluI A'CGCG_T
Cuts at: 297 4223 297
Size: 3926 864
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1208 1262 1373 3461 3645 1208
Size: 54 111 2088 184 2353
Fragments arranged by size:
2353 2088 184 111 54
MscI TGG'CCA
Cuts at: 1685 1780 2551 1685
Size: 95 771 3924
Fragments arranged by size:
3924 771 95
MslI CAynn'nnrTG
Cuts at: 364 1691 2779 2906 364
Size: 1327 1088 127 2248
Fragments arranged by size:
2248 1327 1088 127
MspA1I CmG'CkG
Cuts at: 218 1901 2215 2575 3588 3833 218
Size: 1683 314 360 1013 245 1175
Fragments arranged by size:
1683 1175 1013 360 314 245
NaeI GCC'GGC
Cuts at: 522 817 968 1094 1386 2971 522
Size: 295 151 126 292 1585 2341
Fragments arranged by size:
2341 1585 295 292 151 126
NarI GG'CG_CC
Cuts at: 838 2221 2469 838
Size: 1383 248 3159
Fragments arranged by size:
3159 1383 248
NcoI C'CATG_G
Cuts at: 107 1319 107
Size: 1212 3578
NgoMIV G'CCGG_C
Cuts at: 520 815 966 1092 1384 2969 520
Size: 295 151 126 292 1585 2341
Fragments arranged by size:
2341 1585 295 292 151 126
NspI r_CATG'y
Cuts at: 1843 2343 3250 1843
Size: 500 907 3383
Fragments arranged by size:
3383 907 500
PciI A'CATG_T
Cuts at: 3246 3246
Size: 4790
PfoI T'CCnGG_A
Cuts at: 1030 1030
Size: 4790
PmeI GTTT'AAAC
Cuts at: 1789 1789
Size: 4790
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3929 3961 3929
Size: 32 4758
PpuMI rG'GwC_Cy
Cuts at: 1028 1306 1329 1028
Size: 278 23 4489
Fragments arranged by size:
4489 278 23
PspOMI G'GGCC_C
Cuts at: 919 919
Size: 4790
PstI C_TGCA'G
Cuts at: 1337 1546 1655 1760 1837 2522 1337
Size: 209 109 105 77 685 3605
Fragments arranged by size:
3605 685 209 109 105 77
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4790
PvuII CAG'CTG
Cuts at: 2215 2575 2215
Size: 360 4430
RsrII CG'GwC_CG
Cuts at: 2985 2985
Size: 4790
SacI G_AGCT'C
Cuts at: 748 1804 748
Size: 1056 3734
SacII CC_GC'GG
Cuts at: 219 219
Size: 4790
SalI G'TCGA_C
Cuts at: 1827 1827
Size: 4790
SanDI GG'GwC_CC
Cuts at: 1028 1028
Size: 4790
SapI GCTCTTCn'nnn_
Cuts at: 1006 2813 3023 1006
Size: 1807 210 2773
Fragments arranged by size:
2773 1807 210
SbfI CC_TGCA'GG
Cuts at: 1837 1837
Size: 4790
ScaI AGT'ACT
Cuts at: 4307 4307
Size: 4790
SfcI C'TryA_G
Cuts at: 33 1333 1542 1651 1756 1833 2518 3511 3702
Size: 1300 209 109 105 77 685 993 191
Cuts at: 3702 33
Size: 1121
Fragments arranged by size:
1300 1121 993 685 209 191 109 105
77
SfiI GGCCn_nnn'nGGCC
Cuts at: 1162 1162
Size: 4790
SfoI GGC'GCC
Cuts at: 839 2222 2470 839
Size: 1383 248 3159
Fragments arranged by size:
3159 1383 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4790
SmaI CCC'GGG
Cuts at: 949 1150 1400 1812 949
Size: 201 250 412 3927
Fragments arranged by size:
3927 412 250 201
SmlI C'TyrA_G
Cuts at: 1564 1945 2860 3352 3614 1564
Size: 381 915 492 262 2740
Fragments arranged by size:
2740 915 492 381 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4790
SphI G_CATG'C
Cuts at: 1843 2343 1843
Size: 500 4290
StuI AGG'CCT
Cuts at: 1201 1588 1201
Size: 387 4403
StyI C'CwwG_G
Cuts at: 107 650 701 752 785 824 848 911 1097
Size: 543 51 51 33 39 24 63 186
Cuts at: 1097 1302 1319 1924 2278 107
Size: 205 17 605 354 2619
Fragments arranged by size:
2619 605 543 354 205 186 63 51
51 39 33 24 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2373 2506 2965 3174 2373
Size: 133 459 209 3989
Fragments arranged by size:
3989 459 209 133
TatI w'GTAC_w
Cuts at: 4305 4305
Size: 4790
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 103 135 4307 4339 103
Size: 32 4172 32 554
Fragments arranged by size:
4172 554 32 32
Tth111I GACn'n_nGTC
Cuts at: 2587 2587
Size: 4790
XbaI T'CTAG_A
Cuts at: 1821 1821
Size: 4790
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1164 2285 1164
Size: 1121 3669
XmaI C'CCGG_G
Cuts at: 947 1148 1398 1810 947
Size: 201 250 412 3927
Fragments arranged by size:
3927 412 250 201
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI AscI AvrII BaeI
BbvCI BfrBI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI EcoRV FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspXI PsrI SexAI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI