Restriction map for
pF1KB9751
(Circular) (Six-base) MAPSORT of: pF1KB9751.seq Check: 8408 from: 1 to: 4931
pF1KB9751 4931 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1969 1969
Size: 4931
Acc65I G'GTAC_C
Cuts at: 1008 1947 1008
Size: 939 3992
AclI AA'CG_TT
Cuts at: 4771 4771
Size: 4931
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1026 1527 2756 3188 3935 4203 1026
Size: 501 1229 432 747 268 1754
Fragments arranged by size:
1754 1229 747 501 432 268
AfeI AGC'GCT
Cuts at: 1384 1384
Size: 4931
AflIII A'CryG_T
Cuts at: 2283 3387 4364 2283
Size: 1104 977 2850
Fragments arranged by size:
2850 1104 977
AgeI A'CCGG_T
Cuts at: 3319 3319
Size: 4931
AlwNI CAG_nnn'CTG
Cuts at: 2236 3803 2236
Size: 1567 3364
ApaLI G'TGCA_C
Cuts at: 3701 3701
Size: 4931
ApoI r'AATT_y
Cuts at: 160 271 294 510 785 1093 1131 1313 1397
Size: 111 23 216 275 308 38 182 84
Cuts at: 1397 1817 1935 4284 4540 160
Size: 420 118 2349 256 551
Fragments arranged by size:
2349 551 420 308 275 256 216 182
118 111 84 38 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 4931
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4931
AvaI C'yCGr_G
Cuts at: 1951 1951
Size: 4931
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 428 461 428
Size: 33 4898
BamHI G'GATC_C
Cuts at: 1956 1956
Size: 4931
BanI G'GyrC_C
Cuts at: 328 1008 1947 2361 2609 2644 328
Size: 680 939 414 248 35 2615
Fragments arranged by size:
2615 939 680 414 248 35
BanII G_rGCy'C
Cuts at: 970 1303 1945 2975 970
Size: 333 642 1030 2926
Fragments arranged by size:
2926 1030 642 333
BbeI G_GCGC'C
Cuts at: 332 2365 2613 332
Size: 2033 248 2650
Fragments arranged by size:
2650 2033 248
BbsI GAAGACnn'nnnn_
Cuts at: 734 1051 1364 734
Size: 317 313 4301
Fragments arranged by size:
4301 317 313
BciVI GTATCCnnnnn_n'
Cuts at: 2829 3590 4931 2829
Size: 761 1341 2829
Fragments arranged by size:
2829 1341 761
BclI T'GATC_A
Cuts at: 1881 2451 1881
Size: 570 4361
BfrBI ATG'CAT
Cuts at: 1027 1027
Size: 4931
BglII A'GATC_T
Cuts at: 1049 1473 2446 4483 1049
Size: 424 973 2037 1497
Fragments arranged by size:
2037 1497 973 424
BlpI GC'TnA_GC
Cuts at: 1806 2043 1806
Size: 237 4694
Bme1580I G_kGCm'C
Cuts at: 2556 2649 3705 2556
Size: 93 1056 3782
Fragments arranged by size:
3782 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 405 676 2143 2224 2559 4703 405
Size: 271 1467 81 335 2144 633
Fragments arranged by size:
2144 1467 633 335 271 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 859 3298 859
Size: 2439 2492
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 854 1607 854
Size: 753 4178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 533 2107 2986 3478 3776 533
Size: 1574 879 492 298 1688
Fragments arranged by size:
1688 1574 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4635 4635
Size: 4931
BsaAI yAC'GTr
Cuts at: 2284 2284
Size: 4931
BsaBI GATnn'nnATC
Cuts at: 6 2445 4482 6
Size: 2439 2037 455
Fragments arranged by size:
2439 2037 455
BsaHI Gr'CG_yC
Cuts at: 329 2362 2610 3312 4808 329
Size: 2033 248 702 1496 452
Fragments arranged by size:
2033 1496 702 452 248
BsaWI w'CCGG_w
Cuts at: 970 2120 2641 3319 3593 3740 4151 970
Size: 1150 521 678 274 147 411 1750
Fragments arranged by size:
1750 1150 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 187 217 187
Size: 30 4901
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 166 166
Size: 4931
BseYI C'CCAG_C
Cuts at: 1379 2356 3114 3691 4187 1379
Size: 977 758 577 496 2123
Fragments arranged by size:
2123 977 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2473 2519 3727 104
Size: 2369 46 1208 1308
Fragments arranged by size:
2369 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 970 1303 1945 2723 2913 3705 970
Size: 333 642 778 190 792 2196
Fragments arranged by size:
2196 792 778 642 333 190
BsmI GAATG_Cn'
Cuts at: 327 669 1481 327
Size: 342 812 3777
Fragments arranged by size:
3777 812 342
Bsp1286I G_dGCh'C
Cuts at: 970 1303 1945 2556 2649 2723 2913 2975 3705
Size: 333 642 611 93 74 190 62 730
Cuts at: 3705 970
Size: 2196
Fragments arranged by size:
2196 730 642 611 333 190 93 74
62
BspEI T'CCGG_A
Cuts at: 970 4151 970
Size: 3181 1750
BspHI T'CATG_A
Cuts at: 1692 4107 1692
Size: 2415 2516
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1981 2195 2497 2878 1981
Size: 214 302 381 4034
Fragments arranged by size:
4034 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3223 3277 4761 4926 50
Size: 3173 54 1484 165 55
Fragments arranged by size:
3173 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2843 2843
Size: 4931
BsrFI r'CCGG_y
Cuts at: 2929 3110 3319 2929
Size: 181 209 4541
Fragments arranged by size:
4541 209 181
BssSI C'ACGA_G
Cuts at: 3202 3560 3202
Size: 358 4573
BstAPI GCAn_nnn'nTGC
Cuts at: 2308 2308
Size: 4931
BstBI TT'CG_AA
Cuts at: 1311 1815 3292 1311
Size: 504 1477 2950
Fragments arranged by size:
2950 1477 504
BstYI r'GATC_y
Cuts at: 1 1049 1473 1956 2423 2446 2781 3027 4039
Size: 1048 424 483 467 23 335 246 1012
Cuts at: 4039 4125 4137 4483 1
Size: 86 12 346 449
Fragments arranged by size:
1048 1012 483 467 449 424 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 638 107
Size: 531 4400
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2881 3062 3225 2881
Size: 181 163 4587
Fragments arranged by size:
4587 181 163
BtsI GCAGTG_nn'
Cuts at: 2174 2174
Size: 4931
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 298 333 298
Size: 35 4896
DraI TTT'AAA
Cuts at: 1182 1930 1182
Size: 748 4183
DraIII CAC_nnn'GTG
Cuts at: 808 808
Size: 4931
DrdI GACnn_nn'nnGTC
Cuts at: 2637 3495 2637
Size: 858 4073
EaeI y'GGCC_r
Cuts at: 2516 2690 3081 3108 4453 2516
Size: 174 391 27 1345 2994
Fragments arranged by size:
2994 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2516 2516
Size: 4931
EarI CTCTTCn'nnn_
Cuts at: 1082 1604 1817 1908 2954 3164 1082
Size: 522 213 91 1046 210 2849
Fragments arranged by size:
2849 1046 522 213 210 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3139 3449 3595 4741 3139
Size: 310 146 1146 3329
Fragments arranged by size:
3329 1146 310 146
EcoICRI GAG'CTC
Cuts at: 968 1301 1943 968
Size: 333 642 3956
Fragments arranged by size:
3956 642 333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 854 1026 1527 1607 2756 3188 3935 4203 854
Size: 172 501 80 1149 432 747 268 1582
Fragments arranged by size:
1582 1149 747 501 432 268 172 80
EcoO109I rG'GnC_Cy
Cuts at: 2070 2070
Size: 4931
EcoRI G'AATT_C
Cuts at: 1313 1817 1935 1313
Size: 504 118 4309
Fragments arranged by size:
4309 504 118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2950 2950
Size: 4931
FspI TGC'GCA
Cuts at: 2712 2712
Size: 4931
HaeII r_GCGC'y
Cuts at: 332 1386 2365 2613 3635 4614 332
Size: 1054 979 248 1022 979 649
Fragments arranged by size:
1054 1022 979 979 649 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1041 1073 1194 1226 1290 1322 2773 2803 2805
Size: 32 121 32 64 32 1451 30 2
Cuts at: 2805 2835 1041
Size: 30 3137
Fragments arranged by size:
3137 1451 121 64 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 948 1970 948
Size: 1022 3909
HindIII A'AGCT_T
Cuts at: 1558 1558
Size: 4931
KasI G'GCGC_C
Cuts at: 328 2361 2609 328
Size: 2033 248 2650
Fragments arranged by size:
2650 2033 248
KpnI G_GTAC'C
Cuts at: 1012 1951 1012
Size: 939 3992
MluI A'CGCG_T
Cuts at: 4364 4364
Size: 4931
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3602 3786 3602
Size: 184 4747
MscI TGG'CCA
Cuts at: 2692 2692
Size: 4931
MslI CAynn'nnrTG
Cuts at: 1266 1697 2920 3047 1266
Size: 431 1223 127 3150
Fragments arranged by size:
3150 1223 431 127
MspA1I CmG'CkG
Cuts at: 2042 2356 2716 3729 3974 2042
Size: 314 360 1013 245 2999
Fragments arranged by size:
2999 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3112 3112
Size: 4931
NarI GG'CG_CC
Cuts at: 329 2362 2610 329
Size: 2033 248 2650
Fragments arranged by size:
2650 2033 248
NcoI C'CATG_G
Cuts at: 107 638 107
Size: 531 4400
NgoMIV G'CCGG_C
Cuts at: 3110 3110
Size: 4931
NsiI A_TGCA'T
Cuts at: 1029 1029
Size: 4931
NspI r_CATG'y
Cuts at: 872 1031 1984 2484 3391 872
Size: 159 953 500 907 2412
Fragments arranged by size:
2412 953 907 500 159
PciI A'CATG_T
Cuts at: 3387 3387
Size: 4931
PflMI CCAn_nnn'nTGG
Cuts at: 454 454
Size: 4931
PfoI T'CCnGG_A
Cuts at: 164 622 164
Size: 458 4473
PmeI GTTT'AAAC
Cuts at: 1930 1930
Size: 4931
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 641 673 4070 4102 641
Size: 32 3397 32 1470
Fragments arranged by size:
3397 1470 32 32
PshAI GACnn'nnGTC
Cuts at: 1460 1460
Size: 4931
PsiI TTA'TAA
Cuts at: 1349 1433 1871 1349
Size: 84 438 4409
Fragments arranged by size:
4409 438 84
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1214 1246 1214
Size: 32 4899
PstI C_TGCA'G
Cuts at: 1978 2663 1978
Size: 685 4246
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4931
PvuII CAG'CTG
Cuts at: 2356 2716 2356
Size: 360 4571
RsrII CG'GwC_CG
Cuts at: 3126 3126
Size: 4931
SacI G_AGCT'C
Cuts at: 970 1303 1945 970
Size: 333 642 3956
Fragments arranged by size:
3956 642 333
SalI G'TCGA_C
Cuts at: 1968 1968
Size: 4931
SapI GCTCTTCn'nnn_
Cuts at: 1817 2954 3164 1817
Size: 1137 210 3584
Fragments arranged by size:
3584 1137 210
SbfI CC_TGCA'GG
Cuts at: 1978 1978
Size: 4931
ScaI AGT'ACT
Cuts at: 760 1223 4448 760
Size: 463 3225 1243
Fragments arranged by size:
3225 1243 463
SfcI C'TryA_G
Cuts at: 33 189 1974 2659 3652 3843 33
Size: 156 1785 685 993 191 1121
Fragments arranged by size:
1785 1121 993 685 191 156
SfoI GGC'GCC
Cuts at: 330 2363 2611 330
Size: 2033 248 2650
Fragments arranged by size:
2650 2033 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4931
SmaI CCC'GGG
Cuts at: 1953 1953
Size: 4931
SmlI C'TyrA_G
Cuts at: 548 2086 3001 3493 3755 548
Size: 1538 915 492 262 1724
Fragments arranged by size:
1724 1538 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4931
SphI G_CATG'C
Cuts at: 872 1984 2484 872
Size: 1112 500 3319
Fragments arranged by size:
3319 1112 500
SspI AAT'ATT
Cuts at: 1867 1867
Size: 4931
StyI C'CwwG_G
Cuts at: 107 520 638 2065 2419 107
Size: 413 118 1427 354 2619
Fragments arranged by size:
2619 1427 413 354 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2514 2647 3106 3315 2514
Size: 133 459 209 4130
Fragments arranged by size:
4130 459 209 133
TatI w'GTAC_w
Cuts at: 758 1221 1326 1578 4446 758
Size: 463 105 252 2868 1243
Fragments arranged by size:
2868 1243 463 252 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 187 219 4448 4480 187
Size: 32 4229 32 638
Fragments arranged by size:
4229 638 32 32
Tth111I GACn'n_nGTC
Cuts at: 2728 2728
Size: 4931
XbaI T'CTAG_A
Cuts at: 1962 1962
Size: 4931
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2426 2426
Size: 4931
XmaI C'CCGG_G
Cuts at: 1951 1951
Size: 4931
XmnI GAAnn'nnTTC
Cuts at: 365 695 1540 365
Size: 330 845 3756
Fragments arranged by size:
3756 845 330
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BglI BmgBI BmtI Bpu10I
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI PacI PasI PmlI PpuMI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI