Restriction map for
pF1KB9755
(Circular) (Six-base) MAPSORT of: pF1KB9755.seq Check: 1902 from: 1 to: 5096
pF1KB9755 5096 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 284 284
Size: 5096
AccI GT'mk_AC
Cuts at: 2134 2134
Size: 5096
Acc65I G'GTAC_C
Cuts at: 2112 2112
Size: 5096
AclI AA'CG_TT
Cuts at: 4936 4936
Size: 5096
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 2921 3353 4100 4368 180
Size: 2741 432 747 268 908
Fragments arranged by size:
2741 908 747 432 268
AflIII A'CryG_T
Cuts at: 931 2448 3552 4529 931
Size: 1517 1104 977 1498
Fragments arranged by size:
1517 1498 1104 977
AgeI A'CCGG_T
Cuts at: 3484 3484
Size: 5096
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 617 649 617
Size: 32 5064
AlwNI CAG_nnn'CTG
Cuts at: 155 219 1529 2033 2401 3968 155
Size: 64 1310 504 368 1567 1283
Fragments arranged by size:
1567 1310 1283 504 368 64
ApaLI G'TGCA_C
Cuts at: 3866 3866
Size: 5096
ApoI r'AATT_y
Cuts at: 391 419 628 904 940 989 1131 1325 1409
Size: 28 209 276 36 49 142 194 84
Cuts at: 1409 1493 1577 1725 1829 1913 1977 2100 4449
Size: 84 84 148 104 84 64 123 2349
Cuts at: 4449 4705 391
Size: 256 782
Fragments arranged by size:
2349 782 276 256 209 194 148 142
123 104 84 84 84 84 64 49
36 28
AseI AT'TA_AT
Cuts at: 20 1141 20
Size: 1121 3975
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5096
AvaI C'yCGr_G
Cuts at: 2116 2116
Size: 5096
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 226 259 226
Size: 33 5063
BamHI G'GATC_C
Cuts at: 2121 2121
Size: 5096
BanI G'GyrC_C
Cuts at: 2112 2526 2774 2809 2112
Size: 414 248 35 4399
Fragments arranged by size:
4399 414 248 35
BanII G_rGCy'C
Cuts at: 2110 3140 2110
Size: 1030 4066
BbeI G_GCGC'C
Cuts at: 2530 2778 2530
Size: 248 4848
BbsI GAAGACnn'nnnn_
Cuts at: 467 467
Size: 5096
BbvCI CC'TCA_GC
Cuts at: 871 871
Size: 5096
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5062
BciVI GTATCCnnnnn_n'
Cuts at: 2994 3755 5096 2994
Size: 761 1341 2994
Fragments arranged by size:
2994 1341 761
BclI T'GATC_A
Cuts at: 2616 2616
Size: 5096
BfrBI ATG'CAT
Cuts at: 1149 1401 1569 2073 1149
Size: 252 168 504 4172
Fragments arranged by size:
4172 504 252 168
BglII A'GATC_T
Cuts at: 950 2611 4648 950
Size: 1661 2037 1398
Fragments arranged by size:
2037 1661 1398
BlpI GC'TnA_GC
Cuts at: 1018 2208 1018
Size: 1190 3906
Bme1580I G_kGCm'C
Cuts at: 2721 2814 3870 2721
Size: 93 1056 3947
Fragments arranged by size:
3947 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2308 2389 2724 4868 2308
Size: 81 335 2144 2536
Fragments arranged by size:
2536 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1276 3463 1276
Size: 2187 2909
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1271 1355 1523 1546 1691 1775 2027 1271
Size: 84 168 23 145 84 252 4340
Fragments arranged by size:
4340 252 168 145 84 84 23
Bpu10I CC'TnA_GC
Cuts at: 871 871
Size: 5096
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2272 3151 3643 3941 2272
Size: 879 492 298 3427
Fragments arranged by size:
3427 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4800 4800
Size: 5096
BsaAI yAC'GTr
Cuts at: 2449 2449
Size: 5096
BsaBI GATnn'nnATC
Cuts at: 6 2610 4647 6
Size: 2604 2037 455
Fragments arranged by size:
2604 2037 455
BsaHI Gr'CG_yC
Cuts at: 237 2527 2775 3477 4973 237
Size: 2290 248 702 1496 360
Fragments arranged by size:
2290 1496 702 360 248
BsaWI w'CCGG_w
Cuts at: 2285 2806 3484 3758 3905 4316 2285
Size: 521 678 274 147 411 3065
Fragments arranged by size:
3065 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 429 971 220
Size: 209 542 4345
Fragments arranged by size:
4345 542 209
BseYI C'CCAG_C
Cuts at: 131 2521 3279 3856 4352 131
Size: 2390 758 577 496 875
Fragments arranged by size:
2390 875 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1964 1964
Size: 5096
BsiEI CG_ry'CG
Cuts at: 104 2638 2684 3892 104
Size: 2534 46 1208 1308
Fragments arranged by size:
2534 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2110 2888 3078 3870 2110
Size: 778 190 792 3336
Fragments arranged by size:
3336 792 778 190
BsmI GAATG_Cn'
Cuts at: 1273 1525 1273
Size: 252 4844
Bsp1286I G_dGCh'C
Cuts at: 2110 2721 2814 2888 3078 3140 3870 2110
Size: 611 93 74 190 62 730 3336
Fragments arranged by size:
3336 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4316 4316
Size: 5096
BspHI T'CATG_A
Cuts at: 1011 4272 1011
Size: 3261 1835
BspMI ACCTGCnnnn'nnnn_
Cuts at: 284 2146 2360 2662 3043 284
Size: 1862 214 302 381 2337
Fragments arranged by size:
2337 1862 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3388 3442 4926 5091 50
Size: 3338 54 1484 165 55
Fragments arranged by size:
3338 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 747 1194 3008 747
Size: 447 1814 2835
Fragments arranged by size:
2835 1814 447
BsrFI r'CCGG_y
Cuts at: 3094 3275 3484 3094
Size: 181 209 4706
Fragments arranged by size:
4706 209 181
BssSI C'ACGA_G
Cuts at: 3367 3725 3367
Size: 358 4738
BstAPI GCAn_nnn'nTGC
Cuts at: 2473 2473
Size: 5096
BstBI TT'CG_AA
Cuts at: 293 3457 293
Size: 3164 1932
BstYI r'GATC_y
Cuts at: 1 950 2121 2588 2611 2946 3192 4204 4290
Size: 949 1171 467 23 335 246 1012 86
Cuts at: 4290 4302 4648 1
Size: 12 346 449
Fragments arranged by size:
1171 1012 949 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 343 343
Size: 5096
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2059 3046 3227 3390 2059
Size: 987 181 163 3765
Fragments arranged by size:
3765 987 181 163
BtsI GCAGTG_nn'
Cuts at: 156 1278 1362 1530 2339 156
Size: 1122 84 168 809 2913
Fragments arranged by size:
2913 1122 809 168 84
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1176 1211 1176
Size: 35 5061
DraI TTT'AAA
Cuts at: 2095 2095
Size: 5096
DrdI GACnn_nn'nnGTC
Cuts at: 2802 3660 2802
Size: 858 4238
EaeI y'GGCC_r
Cuts at: 2681 2855 3246 3273 4618 2681
Size: 174 391 27 1345 3159
Fragments arranged by size:
3159 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2681 2681
Size: 5096
EarI CTCTTCn'nnn_
Cuts at: 691 804 3119 3329 691
Size: 113 2315 210 2458
Fragments arranged by size:
2458 2315 210 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3304 3614 3760 4906 3304
Size: 310 146 1146 3494
Fragments arranged by size:
3494 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2108 2108
Size: 5096
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 1271 1355 1523 1546 1691 1775 2027 2921
Size: 1091 84 168 23 145 84 252 894
Cuts at: 2921 3353 4100 4368 180
Size: 432 747 268 908
Fragments arranged by size:
1091 908 894 747 432 268 252 168
145 84 84 23
EcoNI CCTnn'n_nnAGG
Cuts at: 244 244
Size: 5096
EcoO109I rG'GnC_Cy
Cuts at: 1427 1790 2235 1427
Size: 363 445 4288
Fragments arranged by size:
4288 445 363
EcoRI G'AATT_C
Cuts at: 1131 1409 1493 1577 1829 1913 2100 1131
Size: 278 84 84 252 84 187 4127
Fragments arranged by size:
4127 278 252 187 84 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1491 3115 1491
Size: 1624 3472
FspI TGC'GCA
Cuts at: 2877 2877
Size: 5096
HaeII r_GCGC'y
Cuts at: 2530 2778 3800 4779 2530
Size: 248 1022 979 2847
Fragments arranged by size:
2847 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1470 1502 1806 1838 2938 2968 2970 3000 1470
Size: 32 304 32 1100 30 2 30 3566
Fragments arranged by size:
3566 1100 304 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2135 2135
Size: 5096
KasI G'GCGC_C
Cuts at: 2526 2774 2526
Size: 248 4848
KpnI G_GTAC'C
Cuts at: 2116 2116
Size: 5096
MluI A'CGCG_T
Cuts at: 4529 4529
Size: 5096
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 239 305 3767 3951 239
Size: 66 3462 184 1384
Fragments arranged by size:
3462 1384 184 66
MscI TGG'CCA
Cuts at: 2857 2857
Size: 5096
MslI CAynn'nnrTG
Cuts at: 149 680 1082 1938 3085 3212 149
Size: 531 402 856 1147 127 2033
Fragments arranged by size:
2033 1147 856 531 402 127
MspA1I CmG'CkG
Cuts at: 2207 2521 2881 3894 4139 2207
Size: 314 360 1013 245 3164
Fragments arranged by size:
3164 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3277 3277
Size: 5096
NarI GG'CG_CC
Cuts at: 2527 2775 2527
Size: 248 4848
NcoI C'CATG_G
Cuts at: 343 343
Size: 5096
NdeI CA'TA_TG
Cuts at: 1099 1099
Size: 5096
NgoMIV G'CCGG_C
Cuts at: 3275 3275
Size: 5096
NsiI A_TGCA'T
Cuts at: 1151 1403 1571 2075 1151
Size: 252 168 504 4172
Fragments arranged by size:
4172 504 252 168
NspI r_CATG'y
Cuts at: 935 2149 2649 3556 935
Size: 1214 500 907 2475
Fragments arranged by size:
2475 1214 907 500
PciI A'CATG_T
Cuts at: 931 3552 931
Size: 2621 2475
PmeI GTTT'AAAC
Cuts at: 2095 2095
Size: 5096
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4235 4267 4235
Size: 32 5064
PpuMI rG'GwC_Cy
Cuts at: 1427 1427
Size: 5096
PsiI TTA'TAA
Cuts at: 1853 1853
Size: 5096
PstI C_TGCA'G
Cuts at: 2143 2828 2143
Size: 685 4411
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5096
PvuII CAG'CTG
Cuts at: 2521 2881 2521
Size: 360 4736
RsrII CG'GwC_CG
Cuts at: 3291 3291
Size: 5096
SacI G_AGCT'C
Cuts at: 2110 2110
Size: 5096
SalI G'TCGA_C
Cuts at: 2133 2133
Size: 5096
SapI GCTCTTCn'nnn_
Cuts at: 3119 3329 3119
Size: 210 4886
SbfI CC_TGCA'GG
Cuts at: 2143 2143
Size: 5096
ScaI AGT'ACT
Cuts at: 4613 4613
Size: 5096
SfcI C'TryA_G
Cuts at: 33 267 1838 2139 2824 3817 4008 33
Size: 234 1571 301 685 993 191 1121
Fragments arranged by size:
1571 1121 993 685 301 234 191
SfoI GGC'GCC
Cuts at: 2528 2776 2528
Size: 248 4848
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5096
SmaI CCC'GGG
Cuts at: 2118 2118
Size: 5096
SmlI C'TyrA_G
Cuts at: 2251 3166 3658 3920 2251
Size: 915 492 262 3427
Fragments arranged by size:
3427 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5096
SphI G_CATG'C
Cuts at: 2149 2649 2149
Size: 500 4596
SspI AAT'ATT
Cuts at: 587 2082 587
Size: 1495 3601
StuI AGG'CCT
Cuts at: 1120 1456 1708 1120
Size: 336 252 4508
Fragments arranged by size:
4508 336 252
StyI C'CwwG_G
Cuts at: 343 2230 2584 343
Size: 1887 354 2855
Fragments arranged by size:
2855 1887 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 973 2065 2679 2812 3271 3480 973
Size: 1092 614 133 459 209 2589
Fragments arranged by size:
2589 1092 614 459 209 133
TatI w'GTAC_w
Cuts at: 934 1102 1438 1902 4611 934
Size: 168 336 464 2709 1419
Fragments arranged by size:
2709 1419 464 336 168
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4613 4645 4613
Size: 32 5064
Tth111I GACn'n_nGTC
Cuts at: 2893 2893
Size: 5096
XbaI T'CTAG_A
Cuts at: 2127 2127
Size: 5096
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2591 2591
Size: 5096
XmaI C'CCGG_G
Cuts at: 2116 2116
Size: 5096
XmnI GAAnn'nnTTC
Cuts at: 952 1120 1540 1624 1708 1876 1960 952
Size: 168 420 84 84 168 84 4088
Fragments arranged by size:
4088 420 168 168 84 84 84
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PmeI PpiI PpuMI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI ApaI AscI
AvrII BglI BmgBI BmtI BsaXI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI ZraI