Restriction map for
pF1KB9756
(Circular) (Six-base) MAPSORT of: pF1KB9756.seq Check: 2083 from: 1 to: 5228
pF1KB9756 5228 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 914 2069 2266 914
Size: 1155 197 3876
Fragments arranged by size:
3876 1155 197
Acc65I G'GTAC_C
Cuts at: 2244 2244
Size: 5228
AclI AA'CG_TT
Cuts at: 5068 5068
Size: 5228
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1452 1484 1813 1988 2180 3053 3485 4232 4500
Size: 32 329 175 192 873 432 747 268
Cuts at: 4500 1452
Size: 2180
Fragments arranged by size:
2180 873 747 432 329 268 192 175
32
AfeI AGC'GCT
Cuts at: 204 381 204
Size: 177 5051
AflIII A'CryG_T
Cuts at: 2580 3684 4661 2580
Size: 1104 977 3147
Fragments arranged by size:
3147 1104 977
AgeI A'CCGG_T
Cuts at: 3616 3616
Size: 5228
AhdI GACnn_n'nnGTC
Cuts at: 258 258
Size: 5228
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1412 1412
Size: 5228
AlwNI CAG_nnn'CTG
Cuts at: 895 1405 2533 4100 895
Size: 510 1128 1567 2023
Fragments arranged by size:
2023 1567 1128 510
ApaI G_GGCC'C
Cuts at: 333 407 333
Size: 74 5154
ApaLI G'TGCA_C
Cuts at: 3998 3998
Size: 5228
ApoI r'AATT_y
Cuts at: 943 2124 2232 4581 4837 943
Size: 1181 108 2349 256 1334
Fragments arranged by size:
2349 1334 1181 256 108
AseI AT'TA_AT
Cuts at: 20 2153 20
Size: 2133 3095
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5228
AvaI C'yCGr_G
Cuts at: 715 789 2248 715
Size: 74 1459 3695
Fragments arranged by size:
3695 1459 74
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 619 652 619
Size: 33 5195
BamHI G'GATC_C
Cuts at: 2253 2253
Size: 5228
BanI G'GyrC_C
Cuts at: 618 793 2244 2658 2906 2941 618
Size: 175 1451 414 248 35 2905
Fragments arranged by size:
2905 1451 414 248 175 35
BanII G_rGCy'C
Cuts at: 139 311 333 407 427 529 819 1355 1601
Size: 172 22 74 20 102 290 536 246
Cuts at: 1601 2242 3272 139
Size: 641 1030 2095
Fragments arranged by size:
2095 1030 641 536 290 246 172 102
74 22 20
BbeI G_GCGC'C
Cuts at: 622 2662 2910 622
Size: 2040 248 2940
Fragments arranged by size:
2940 2040 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5194
BciVI GTATCCnnnnn_n'
Cuts at: 3126 3887 5228 3126
Size: 761 1341 3126
Fragments arranged by size:
3126 1341 761
BclI T'GATC_A
Cuts at: 968 1443 2748 968
Size: 475 1305 3448
Fragments arranged by size:
3448 1305 475
BglI GCCn_nnn'nGGC
Cuts at: 337 422 487 850 936 337
Size: 85 65 363 86 4629
Fragments arranged by size:
4629 363 86 85 65
BglII A'GATC_T
Cuts at: 2743 4780 2743
Size: 2037 3191
BlpI GC'TnA_GC
Cuts at: 2340 2340
Size: 5228
Bme1580I G_kGCm'C
Cuts at: 333 407 712 796 909 2853 2946 4002 333
Size: 74 305 84 113 1944 93 1056 1559
Fragments arranged by size:
1944 1559 1056 305 113 93 84 74
BmgBI CAC'GTC
Cuts at: 912 912
Size: 5228
BmrI ACTGGGnnnn_n'
Cuts at: 880 2440 2521 2856 5000 880
Size: 1560 81 335 2144 1108
Fragments arranged by size:
2144 1560 1108 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 468 3595 468
Size: 3127 2101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 529 860 1583 1926 2097 193
Size: 336 331 723 343 171 3324
Fragments arranged by size:
3324 723 343 336 331 171
Bpu10I CC'TnA_GC
Cuts at: 523 523
Size: 5228
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1026 1604 2404 3283 3775 4073 1026
Size: 578 800 879 492 298 2181
Fragments arranged by size:
2181 879 800 578 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4932 4932
Size: 5228
BsaAI yAC'GTr
Cuts at: 2581 2581
Size: 5228
BsaBI GATnn'nnATC
Cuts at: 6 2742 4779 6
Size: 2736 2037 455
Fragments arranged by size:
2736 2037 455
BsaHI Gr'CG_yC
Cuts at: 359 619 2659 2907 3609 5105 359
Size: 260 2040 248 702 1496 482
Fragments arranged by size:
2040 1496 702 482 260 248
BsaWI w'CCGG_w
Cuts at: 2417 2938 3616 3890 4037 4448 2417
Size: 521 678 274 147 411 3197
Fragments arranged by size:
3197 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1509 1521 1539 1551 1509
Size: 12 18 12 5186
Fragments arranged by size:
5186 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 224 454 457 224
Size: 230 3 4995
Fragments arranged by size:
4995 230 3
BseYI C'CCAG_C
Cuts at: 217 613 1709 2653 3411 3988 4484 217
Size: 396 1096 944 758 577 496 961
Fragments arranged by size:
1096 961 944 758 577 496 396
BsiEI CG_ry'CG
Cuts at: 104 417 2770 2816 4024 104
Size: 313 2353 46 1208 1308
Fragments arranged by size:
2353 1308 1208 313 46
BsiHKAI G_wGCw'C
Cuts at: 139 529 2242 3020 3210 4002 139
Size: 390 1713 778 190 792 1365
Fragments arranged by size:
1713 1365 792 778 390 190
Bsp1286I G_dGCh'C
Cuts at: 139 311 333 407 427 529 712 796 819
Size: 172 22 74 20 102 183 84 23
Cuts at: 819 909 1355 1601 2242 2853 2946 3020 3210
Size: 90 446 246 641 611 93 74 190
Cuts at: 3210 3272 4002 139
Size: 62 730 1365
Fragments arranged by size:
1365 730 641 611 446 246 190 183
172 102 93 90 84 74 74 62
23 22 20
BspEI T'CCGG_A
Cuts at: 4448 4448
Size: 5228
BspHI T'CATG_A
Cuts at: 4404 4404
Size: 5228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 155 2278 2492 2794 3175 155
Size: 2123 214 302 381 2208
Fragments arranged by size:
2208 2123 381 302 214
BsrBI CCG'CTC
Cuts at: 50 302 534 822 3520 3574 5058 5223 50
Size: 252 232 288 2698 54 1484 165 55
Fragments arranged by size:
2698 1484 288 252 232 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1394 1795 1883 3140 1394
Size: 401 88 1257 3482
Fragments arranged by size:
3482 1257 401 88
BsrFI r'CCGG_y
Cuts at: 742 926 3226 3407 3616 742
Size: 184 2300 181 209 2354
Fragments arranged by size:
2354 2300 209 184 181
BsrGI T'GTAC_A
Cuts at: 1490 1490
Size: 5228
BssHII G'CGCG_C
Cuts at: 155 502 655 155
Size: 347 153 4728
Fragments arranged by size:
4728 347 153
BssSI C'ACGA_G
Cuts at: 3499 3857 3499
Size: 358 4870
BstAPI GCAn_nnn'nTGC
Cuts at: 1405 2605 1405
Size: 1200 4028
BstBI TT'CG_AA
Cuts at: 3589 3589
Size: 5228
BstEII G'GTnAC_C
Cuts at: 1110 1110
Size: 5228
BstXI CCAn_nnnn'nTGG
Cuts at: 1741 1741
Size: 5228
BstYI r'GATC_y
Cuts at: 1 856 1543 2253 2720 2743 3078 3324 4336
Size: 855 687 710 467 23 335 246 1012
Cuts at: 4336 4422 4434 4780 1
Size: 86 12 346 449
Fragments arranged by size:
1012 855 710 687 467 449 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 2070 2070
Size: 5228
Bsu36I CC'TnA_GG
Cuts at: 560 560
Size: 5228
BtgI C'CryG_G
Cuts at: 335 485 512 624 698 1529 1933 335
Size: 150 27 112 74 831 404 3630
Fragments arranged by size:
3630 831 404 150 112 74 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3178 3359 3522 3178
Size: 181 163 4884
Fragments arranged by size:
4884 181 163
BtsI GCAGTG_nn'
Cuts at: 395 497 759 1034 1406 2471 395
Size: 102 262 275 372 1065 3152
Fragments arranged by size:
3152 1065 372 275 262 102
DraI TTT'AAA
Cuts at: 2227 2227
Size: 5228
DrdI GACnn_nn'nnGTC
Cuts at: 2934 3792 2934
Size: 858 4370
EaeI y'GGCC_r
Cuts at: 207 338 392 414 494 1697 2813 2987 3378
Size: 131 54 22 80 1203 1116 174 391
Cuts at: 3378 3405 4750 207
Size: 27 1345 685
Fragments arranged by size:
1345 1203 1116 685 391 174 131 80
54 27 22
EagI C'GGCC_G
Cuts at: 414 2813 414
Size: 2399 2829
EarI CTCTTCn'nnn_
Cuts at: 598 1685 2016 2156 3251 3461 598
Size: 1087 331 140 1095 210 2365
Fragments arranged by size:
2365 1095 1087 331 210 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 444 1087 3436 3746 3892 5038 444
Size: 643 2349 310 146 1146 634
Fragments arranged by size:
2349 1146 643 634 310 146
EcoICRI GAG'CTC
Cuts at: 137 527 2240 137
Size: 390 1713 3125
Fragments arranged by size:
3125 1713 390
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 529 860 1452 1484 1583 1813 1926 1988
Size: 336 331 592 32 99 230 113 62
Cuts at: 1988 2097 2180 3053 3485 4232 4500 193
Size: 109 83 873 432 747 268 921
Fragments arranged by size:
921 873 747 592 432 336 331 268
230 113 109 99 83 62 32
EcoO109I rG'GnC_Cy
Cuts at: 329 330 403 404 876 2367 329
Size: 1 73 1 472 1491 3190
Fragments arranged by size:
3190 1491 472 73 1 1
EcoRI G'AATT_C
Cuts at: 2232 2232
Size: 5228
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 264 3247 264
Size: 2983 2245
FspI TGC'GCA
Cuts at: 3009 3009
Size: 5228
HaeII r_GCGC'y
Cuts at: 206 383 622 1092 2662 2910 3932 4911 206
Size: 177 239 470 1570 248 1022 979 523
Fragments arranged by size:
1570 1022 979 523 470 248 239 177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 244 276 1963 1995 3070 3100 3102 3132 244
Size: 32 1687 32 1075 30 2 30 2340
Fragments arranged by size:
2340 1687 1075 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1517 1608 2267 1517
Size: 91 659 4478
Fragments arranged by size:
4478 659 91
HpaI GTT'AAC
Cuts at: 1517 1517
Size: 5228
KasI G'GCGC_C
Cuts at: 618 2658 2906 618
Size: 2040 248 2940
Fragments arranged by size:
2940 2040 248
KpnI G_GTAC'C
Cuts at: 2248 2248
Size: 5228
MluI A'CGCG_T
Cuts at: 4661 4661
Size: 5228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 224 548 3899 4083 224
Size: 324 3351 184 1369
Fragments arranged by size:
3351 1369 324 184
MscI TGG'CCA
Cuts at: 209 394 1699 2989 209
Size: 185 1305 1290 2448
Fragments arranged by size:
2448 1305 1290 185
MslI CAynn'nnrTG
Cuts at: 1046 1264 1399 3217 3344 1046
Size: 218 135 1818 127 2930
Fragments arranged by size:
2930 1818 218 135 127
MspA1I CmG'CkG
Cuts at: 171 194 337 460 487 700 773 850 1713
Size: 23 143 123 27 213 73 77 863
Cuts at: 1713 1924 2339 2653 3013 4026 4271 171
Size: 211 415 314 360 1013 245 1128
Fragments arranged by size:
1128 1013 863 415 360 314 245 213
211 143 123 77 73 27 23
NaeI GCC'GGC
Cuts at: 3409 3409
Size: 5228
NarI GG'CG_CC
Cuts at: 619 2659 2907 619
Size: 2040 248 2940
Fragments arranged by size:
2940 2040 248
NcoI C'CATG_G
Cuts at: 512 1933 512
Size: 1421 3807
NdeI CA'TA_TG
Cuts at: 1806 1806
Size: 5228
NgoMIV G'CCGG_C
Cuts at: 3407 3407
Size: 5228
NspI r_CATG'y
Cuts at: 996 1140 2281 2781 3688 996
Size: 144 1141 500 907 2536
Fragments arranged by size:
2536 1141 907 500 144
PasI CC'CwG_GG
Cuts at: 1845 1845
Size: 5228
PciI A'CATG_T
Cuts at: 3684 3684
Size: 5228
PflMI CCAn_nnn'nTGG
Cuts at: 895 1085 1845 895
Size: 190 760 4278
Fragments arranged by size:
4278 760 190
PfoI T'CCnGG_A
Cuts at: 1592 1592
Size: 5228
PmeI GTTT'AAAC
Cuts at: 2227 2227
Size: 5228
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4367 4399 4367
Size: 32 5196
PsiI TTA'TAA
Cuts at: 1634 1634
Size: 5228
PspOMI G'GGCC_C
Cuts at: 329 403 329
Size: 74 5154
PstI C_TGCA'G
Cuts at: 465 2275 2960 465
Size: 1810 685 2733
Fragments arranged by size:
2733 1810 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5228
PvuII CAG'CTG
Cuts at: 1924 2653 3013 1924
Size: 729 360 4139
Fragments arranged by size:
4139 729 360
RsrII CG'GwC_CG
Cuts at: 3423 3423
Size: 5228
SacI G_AGCT'C
Cuts at: 139 529 2242 139
Size: 390 1713 3125
Fragments arranged by size:
3125 1713 390
SacII CC_GC'GG
Cuts at: 338 488 701 338
Size: 150 213 4865
Fragments arranged by size:
4865 213 150
SalI G'TCGA_C
Cuts at: 2265 2265
Size: 5228
SapI GCTCTTCn'nnn_
Cuts at: 2016 3251 3461 2016
Size: 1235 210 3783
Fragments arranged by size:
3783 1235 210
SbfI CC_TGCA'GG
Cuts at: 2275 2275
Size: 5228
ScaI AGT'ACT
Cuts at: 4745 4745
Size: 5228
SfcI C'TryA_G
Cuts at: 33 461 2271 2956 3949 4140 33
Size: 428 1810 685 993 191 1121
Fragments arranged by size:
1810 1121 993 685 428 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 337 337
Size: 5228
SfoI GGC'GCC
Cuts at: 620 2660 2908 620
Size: 2040 248 2940
Fragments arranged by size:
2940 2040 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5228
SmaI CCC'GGG
Cuts at: 717 791 2250 717
Size: 74 1459 3695
Fragments arranged by size:
3695 1459 74
SmlI C'TyrA_G
Cuts at: 1005 1619 2383 3298 3790 4052 1005
Size: 614 764 915 492 262 2181
Fragments arranged by size:
2181 915 764 614 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5228
SphI G_CATG'C
Cuts at: 2281 2781 2281
Size: 500 4728
SrfI GCCC'GGGC
Cuts at: 791 791
Size: 5228
StyI C'CwwG_G
Cuts at: 512 1933 1982 2362 2716 512
Size: 1421 49 380 354 3024
Fragments arranged by size:
3024 1421 380 354 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1098 1178 1276 2811 2944 3403 3612 1098
Size: 80 98 1535 133 459 209 2714
Fragments arranged by size:
2714 1535 459 209 133 98 80
TatI w'GTAC_w
Cuts at: 803 1490 4743 803
Size: 687 3253 1288
Fragments arranged by size:
3253 1288 687
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1521 1553 4745 4777 1521
Size: 32 3192 32 1972
Fragments arranged by size:
3192 1972 32 32
Tth111I GACn'n_nGTC
Cuts at: 3025 3025
Size: 5228
XbaI T'CTAG_A
Cuts at: 2046 2259 2046
Size: 213 5015
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 610 2723 610
Size: 2113 3115
XmaI C'CCGG_G
Cuts at: 715 789 2248 715
Size: 74 1459 3695
Fragments arranged by size:
3695 1459 74
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AloI AscI AvrII BbsI
BbvCI BfrBI BmtI BsgI BsiWI BsmI BsmBI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII MfeI
NheI NotI NruI NsiI PacI PmlI PpuMI PshAI
PspXI PsrI SanDI SexAI SgrAI SnaBI SspI StuI
SwaI XhoI XmnI ZraI