Restriction map for
pF1KB9758
(Circular) (Six-base) MAPSORT of: pF1KB9758.seq Check: 9604 from: 1 to: 5246
pF1KB9758 5246 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 949 1171 1290 949
Size: 222 119 4905
Fragments arranged by size:
4905 222 119
AccI GT'mk_AC
Cuts at: 338 389 2284 338
Size: 51 1895 3300
Fragments arranged by size:
3300 1895 51
Acc65I G'GTAC_C
Cuts at: 2262 2262
Size: 5246
AclI AA'CG_TT
Cuts at: 5086 5086
Size: 5246
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 671 1126 1216 3071 3503 4250 4518 671
Size: 455 90 1855 432 747 268 1399
Fragments arranged by size:
1855 1399 747 455 432 268 90
AfeI AGC'GCT
Cuts at: 1370 1370
Size: 5246
AflIII A'CryG_T
Cuts at: 1591 2598 3702 4679 1591
Size: 1007 1104 977 2158
Fragments arranged by size:
2158 1104 1007 977
AgeI A'CCGG_T
Cuts at: 3634 3634
Size: 5246
AleI CACnn'nnGTG
Cuts at: 197 629 1141 1565 2134 197
Size: 432 512 424 569 3309
Fragments arranged by size:
3309 569 512 432 424
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 872 872
Size: 5246
AlwNI CAG_nnn'CTG
Cuts at: 964 2551 4118 964
Size: 1587 1567 2092
Fragments arranged by size:
2092 1587 1567
ApaLI G'TGCA_C
Cuts at: 1216 4016 1216
Size: 2800 2446
ApoI r'AATT_y
Cuts at: 531 1472 1701 2250 4599 4855 531
Size: 941 229 549 2349 256 922
Fragments arranged by size:
2349 941 922 549 256 229
AseI AT'TA_AT
Cuts at: 20 20
Size: 5246
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5246
AvaI C'yCGr_G
Cuts at: 2266 2266
Size: 5246
BamHI G'GATC_C
Cuts at: 1651 2271 1651
Size: 620 4626
BanI G'GyrC_C
Cuts at: 1325 1429 2262 2676 2924 2959 1325
Size: 104 833 414 248 35 3612
Fragments arranged by size:
3612 833 414 248 104 35
BanII G_rGCy'C
Cuts at: 2260 3290 2260
Size: 1030 4216
BbeI G_GCGC'C
Cuts at: 1433 2680 2928 1433
Size: 1247 248 3751
Fragments arranged by size:
3751 1247 248
BbsI GAAGACnn'nnnn_
Cuts at: 677 840 677
Size: 163 5083
BbvCI CC'TCA_GC
Cuts at: 860 860
Size: 5246
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 160 194 160
Size: 34 5212
BciVI GTATCCnnnnn_n'
Cuts at: 3144 3905 5246 3144
Size: 761 1341 3144
Fragments arranged by size:
3144 1341 761
BclI T'GATC_A
Cuts at: 2766 2766
Size: 5246
BfrBI ATG'CAT
Cuts at: 171 171
Size: 5246
BglI GCCn_nnn'nGGC
Cuts at: 256 598 1343 2119 256
Size: 342 745 776 3383
Fragments arranged by size:
3383 776 745 342
BglII A'GATC_T
Cuts at: 2761 4798 2761
Size: 2037 3209
BlpI GC'TnA_GC
Cuts at: 2176 2358 2176
Size: 182 5064
Bme1580I G_kGCm'C
Cuts at: 919 1220 2871 2964 4020 919
Size: 301 1651 93 1056 2145
Fragments arranged by size:
2145 1651 1056 301 93
BmgBI CAC'GTC
Cuts at: 204 1281 204
Size: 1077 4169
BmrI ACTGGGnnnn_n'
Cuts at: 623 2458 2539 2874 5018 623
Size: 1835 81 335 2144 851
Fragments arranged by size:
2144 1835 851 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3613 3613
Size: 5246
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1777 1777
Size: 5246
Bpu10I CC'TnA_GC
Cuts at: 860 860
Size: 5246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 1441 2422 3301 3793 4091 400
Size: 1041 981 879 492 298 1555
Fragments arranged by size:
1555 1041 981 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 640 1394 4950 640
Size: 754 3556 936
Fragments arranged by size:
3556 936 754
BsaAI yAC'GTr
Cuts at: 1215 1293 2599 1215
Size: 78 1306 3862
Fragments arranged by size:
3862 1306 78
BsaBI GATnn'nnATC
Cuts at: 6 2760 4797 6
Size: 2754 2037 455
Fragments arranged by size:
2754 2037 455
BsaHI Gr'CG_yC
Cuts at: 1430 2677 2925 3627 5123 1430
Size: 1247 248 702 1496 1553
Fragments arranged by size:
1553 1496 1247 702 248
BsaWI w'CCGG_w
Cuts at: 2435 2956 3634 3908 4055 4466 2435
Size: 521 678 274 147 411 3215
Fragments arranged by size:
3215 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 759 789 964 994 759
Size: 30 175 30 5011
Fragments arranged by size:
5011 175 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1015 1024 1027 1030 1033 1036 1015
Size: 9 3 3 3 3 5225
Fragments arranged by size:
5225 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 787 1694 1778 2671 3429 4006 4502 787
Size: 907 84 893 758 577 496 1531
Fragments arranged by size:
1531 907 893 758 577 496 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 373 445 1083 1363 1401 140
Size: 233 72 638 280 38 3985
Fragments arranged by size:
3985 638 280 233 72 38
BsiEI CG_ry'CG
Cuts at: 104 2788 2834 4042 104
Size: 2684 46 1208 1308
Fragments arranged by size:
2684 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1220 2260 3038 3228 4020 1220
Size: 1040 778 190 792 2446
Fragments arranged by size:
2446 1040 792 778 190
BsmI GAATG_Cn'
Cuts at: 437 437
Size: 5246
Bsp1286I G_dGCh'C
Cuts at: 919 1220 2260 2871 2964 3038 3228 3290 4020
Size: 301 1040 611 93 74 190 62 730
Cuts at: 4020 919
Size: 2145
Fragments arranged by size:
2145 1040 730 611 301 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4466 4466
Size: 5246
BspHI T'CATG_A
Cuts at: 4422 4422
Size: 5246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 345 921 949 1171 1290 2296 2510 2812 3193
Size: 576 28 222 119 1006 214 302 381
Cuts at: 3193 345
Size: 2398
Fragments arranged by size:
2398 1006 576 381 302 222 214 119
28
BsrBI CCG'CTC
Cuts at: 50 1323 3538 3592 5076 5241 50
Size: 1273 2215 54 1484 165 55
Fragments arranged by size:
2215 1484 1273 165 55 54
BsrDI GCAATG_nn'
Cuts at: 681 1845 3158 681
Size: 1164 1313 2769
Fragments arranged by size:
2769 1313 1164
BsrFI r'CCGG_y
Cuts at: 1403 3244 3425 3634 1403
Size: 1841 181 209 3015
Fragments arranged by size:
3015 1841 209 181
BssSI C'ACGA_G
Cuts at: 1381 3517 3875 1381
Size: 2136 358 2752
Fragments arranged by size:
2752 2136 358
BstAPI GCAn_nnn'nTGC
Cuts at: 168 432 1070 1296 1424 1844 2623 168
Size: 264 638 226 128 420 779 2791
Fragments arranged by size:
2791 779 638 420 264 226 128
BstBI TT'CG_AA
Cuts at: 3607 3607
Size: 5246
BstEII G'GTnAC_C
Cuts at: 190 190
Size: 5246
BstXI CCAn_nnnn'nTGG
Cuts at: 1143 1143
Size: 5246
BstYI r'GATC_y
Cuts at: 1 809 1651 2271 2738 2761 3096 3342 4354
Size: 808 842 620 467 23 335 246 1012
Cuts at: 4354 4440 4452 4798 1
Size: 86 12 346 449
Fragments arranged by size:
1012 842 808 620 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 1125 1912 2132 107
Size: 1018 787 220 3221
Fragments arranged by size:
3221 1018 787 220
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3196 3377 3540 3196
Size: 181 163 4902
Fragments arranged by size:
4902 181 163
BtsI GCAGTG_nn'
Cuts at: 266 392 570 2489 266
Size: 126 178 1919 3023
Fragments arranged by size:
3023 1919 178 126
DraI TTT'AAA
Cuts at: 1665 2245 1665
Size: 580 4666
DraIII CAC_nnn'GTG
Cuts at: 1131 1424 1131
Size: 293 4953
DrdI GACnn_nn'nnGTC
Cuts at: 208 2952 3810 208
Size: 2744 858 1644
Fragments arranged by size:
2744 1644 858
EaeI y'GGCC_r
Cuts at: 608 1133 1167 1335 2040 2156 2831 3005 3396
Size: 525 34 168 705 116 675 174 391
Cuts at: 3396 3423 4768 608
Size: 27 1345 1086
Fragments arranged by size:
1345 1086 705 675 525 391 174 168
116 34 27
EagI C'GGCC_G
Cuts at: 2831 2831
Size: 5246
EarI CTCTTCn'nnn_
Cuts at: 304 1001 1042 1090 1192 1880 1982 3269 3479
Size: 697 41 48 102 688 102 1287 210
Cuts at: 3479 304
Size: 2071
Fragments arranged by size:
2071 1287 697 688 210 102 102 48
41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3454 3764 3910 5056 3454
Size: 310 146 1146 3644
Fragments arranged by size:
3644 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2258 2258
Size: 5246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 671 1126 1216 1777 3071 3503 4250 4518 671
Size: 455 90 561 1294 432 747 268 1399
Fragments arranged by size:
1399 1294 747 561 455 432 268 90
EcoO109I rG'GnC_Cy
Cuts at: 1350 2385 1350
Size: 1035 4211
EcoRI G'AATT_C
Cuts at: 1472 1701 2250 1472
Size: 229 549 4468
Fragments arranged by size:
4468 549 229
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 316 1071 1638 3265 316
Size: 755 567 1627 2297
Fragments arranged by size:
2297 1627 755 567
FspI TGC'GCA
Cuts at: 3027 3027
Size: 5246
HaeII r_GCGC'y
Cuts at: 1212 1233 1372 1433 1800 2680 2928 3950 4929
Size: 21 139 61 367 880 248 1022 979
Cuts at: 4929 1212
Size: 1529
Fragments arranged by size:
1529 1022 979 880 367 248 139 61
21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 964 996 1981 2013 3088 3118 3120 3150 964
Size: 32 985 32 1075 30 2 30 3060
Fragments arranged by size:
3060 1075 985 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2285 2285
Size: 5246
HindIII A'AGCT_T
Cuts at: 2005 2005
Size: 5246
KasI G'GCGC_C
Cuts at: 1429 2676 2924 1429
Size: 1247 248 3751
Fragments arranged by size:
3751 1247 248
KpnI G_GTAC'C
Cuts at: 2266 2266
Size: 5246
MluI A'CGCG_T
Cuts at: 4679 4679
Size: 5246
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 950 3917 4101 950
Size: 2967 184 2095
Fragments arranged by size:
2967 2095 184
MscI TGG'CCA
Cuts at: 610 1135 2042 2158 3007 610
Size: 525 907 116 849 2849
Fragments arranged by size:
2849 907 849 525 116
MslI CAynn'nnrTG
Cuts at: 197 629 922 1141 1565 1833 2134 3235 3362
Size: 432 293 219 424 268 301 1101 127
Cuts at: 3362 197
Size: 2081
Fragments arranged by size:
2081 1101 432 424 301 293 268 219
127
MspA1I CmG'CkG
Cuts at: 1154 1187 1331 1698 2357 2671 3031 4044 4289
Size: 33 144 367 659 314 360 1013 245
Cuts at: 4289 1154
Size: 2111
Fragments arranged by size:
2111 1013 659 367 360 314 245 144
33
NaeI GCC'GGC
Cuts at: 3427 3427
Size: 5246
NarI GG'CG_CC
Cuts at: 1430 2677 2925 1430
Size: 1247 248 3751
Fragments arranged by size:
3751 1247 248
NcoI C'CATG_G
Cuts at: 107 1912 107
Size: 1805 3441
NgoMIV G'CCGG_C
Cuts at: 3425 3425
Size: 5246
NsiI A_TGCA'T
Cuts at: 173 173
Size: 5246
NspI r_CATG'y
Cuts at: 684 1539 1595 2299 2799 3706 684
Size: 855 56 704 500 907 2224
Fragments arranged by size:
2224 907 855 704 500 56
PasI CC'CwG_GG
Cuts at: 596 596
Size: 5246
PciI A'CATG_T
Cuts at: 1591 3702 1591
Size: 2111 3135
PflMI CCAn_nnn'nTGG
Cuts at: 1131 2055 1131
Size: 924 4322
PmeI GTTT'AAAC
Cuts at: 2245 2245
Size: 5246
PmlI CAC'GTG
Cuts at: 1215 1293 1215
Size: 78 5168
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4385 4417 4385
Size: 32 5214
PshAI GACnn'nnGTC
Cuts at: 334 334
Size: 5246
PstI C_TGCA'G
Cuts at: 1301 2064 2293 2978 1301
Size: 763 229 685 3569
Fragments arranged by size:
3569 763 685 229
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5246
PvuII CAG'CTG
Cuts at: 2671 3031 2671
Size: 360 4886
RsrII CG'GwC_CG
Cuts at: 3441 3441
Size: 5246
SacI G_AGCT'C
Cuts at: 2260 2260
Size: 5246
SalI G'TCGA_C
Cuts at: 2283 2283
Size: 5246
SapI GCTCTTCn'nnn_
Cuts at: 1042 1090 1192 3269 3479 1042
Size: 48 102 2077 210 2809
Fragments arranged by size:
2809 2077 210 102 48
SbfI CC_TGCA'GG
Cuts at: 2293 2293
Size: 5246
ScaI AGT'ACT
Cuts at: 515 1621 4763 515
Size: 1106 3142 998
Fragments arranged by size:
3142 1106 998
SfcI C'TryA_G
Cuts at: 33 879 1297 2060 2289 2974 3967 4158 33
Size: 846 418 763 229 685 993 191 1121
Fragments arranged by size:
1121 993 846 763 685 418 229 191
SfoI GGC'GCC
Cuts at: 1431 2678 2926 1431
Size: 1247 248 3751
Fragments arranged by size:
3751 1247 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5246
SmaI CCC'GGG
Cuts at: 2268 2268
Size: 5246
SmlI C'TyrA_G
Cuts at: 379 1456 2401 3316 3808 4070 379
Size: 1077 945 915 492 262 1555
Fragments arranged by size:
1555 1077 945 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5246
SphI G_CATG'C
Cuts at: 2299 2799 2299
Size: 500 4746
StyI C'CwwG_G
Cuts at: 107 647 710 1912 2380 2734 107
Size: 540 63 1202 468 354 2619
Fragments arranged by size:
2619 1202 540 468 354 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1792 2829 2962 3421 3630 1792
Size: 1037 133 459 209 3408
Fragments arranged by size:
3408 1037 459 209 133
TatI w'GTAC_w
Cuts at: 513 1619 4761 513
Size: 1106 3142 998
Fragments arranged by size:
3142 1106 998
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 759 791 868 900 4763 4795 759
Size: 32 77 32 3863 32 1210
Fragments arranged by size:
3863 1210 77 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3043 3043
Size: 5246
XbaI T'CTAG_A
Cuts at: 2277 2277
Size: 5246
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 603 2741 603
Size: 2138 3108
XmaI C'CCGG_G
Cuts at: 2266 2266
Size: 5246
XmnI GAAnn'nnTTC
Cuts at: 1099 1261 1921 1099
Size: 162 660 4424
Fragments arranged by size:
4424 660 162
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI ApaI AscI AvrII BaeI
BmtI BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI PacI PfoI PpuMI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI