Restriction map for
pF1KB9764
(Circular) (Six-base) MAPSORT of: pF1KB9764.seq Check: 9000 from: 1 to: 5630
pF1KB9764 5630 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 2668 2668
Size: 5630
Acc65I G'GTAC_C
Cuts at: 2646 2646
Size: 5630
AclI AA'CG_TT
Cuts at: 1053 5470 1053
Size: 4417 1213
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 483 572 655 1740 3455 3887 4634 4902 483
Size: 89 83 1085 1715 432 747 268 1211
Fragments arranged by size:
1715 1211 1085 747 432 268 89 83
AflIII A'CryG_T
Cuts at: 1006 2570 2982 4086 5063 1006
Size: 1564 412 1104 977 1573
Fragments arranged by size:
1573 1564 1104 977 412
AgeI A'CCGG_T
Cuts at: 4018 4018
Size: 5630
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 226 258 226
Size: 32 5598
AlwNI CAG_nnn'CTG
Cuts at: 2935 4502 2935
Size: 1567 4063
ApaLI G'TGCA_C
Cuts at: 4400 4400
Size: 5630
ApoI r'AATT_y
Cuts at: 154 391 716 924 1092 1118 1176 1202 1344
Size: 237 325 208 168 26 58 26 142
Cuts at: 1344 1370 1680 1706 1848 1874 1958 2100 2352
Size: 26 310 26 142 26 84 142 252
Cuts at: 2352 2378 2462 2619 2634 4983 5239 154
Size: 26 84 157 15 2349 256 545
Fragments arranged by size:
2349 545 325 310 256 252 237 208
168 157 142 142 142 84 84 58
26 26 26 26 26 26 15
AseI AT'TA_AT
Cuts at: 20 20
Size: 5630
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5630
AvaI C'yCGr_G
Cuts at: 2650 2650
Size: 5630
BamHI G'GATC_C
Cuts at: 2655 2655
Size: 5630
BanI G'GyrC_C
Cuts at: 2646 3060 3308 3343 2646
Size: 414 248 35 4933
Fragments arranged by size:
4933 414 248 35
BanII G_rGCy'C
Cuts at: 1260 2644 3674 1260
Size: 1384 1030 3216
Fragments arranged by size:
3216 1384 1030
BbeI G_GCGC'C
Cuts at: 3064 3312 3064
Size: 248 5382
BbsI GAAGACnn'nnnn_
Cuts at: 1158 1326 1662 1830 2082 2334 1158
Size: 168 336 168 252 252 4454
Fragments arranged by size:
4454 336 252 252 168 168
BciVI GTATCCnnnnn_n'
Cuts at: 3528 4289 5630 3528
Size: 761 1341 3528
Fragments arranged by size:
3528 1341 761
BclI T'GATC_A
Cuts at: 371 3150 371
Size: 2779 2851
BglII A'GATC_T
Cuts at: 3145 5182 3145
Size: 2037 3593
BlpI GC'TnA_GC
Cuts at: 1269 2742 1269
Size: 1473 4157
Bme1580I G_kGCm'C
Cuts at: 3255 3348 4404 3255
Size: 93 1056 4481
Fragments arranged by size:
4481 1056 93
BmgBI CAC'GTC
Cuts at: 213 213
Size: 5630
BmrI ACTGGGnnnn_n'
Cuts at: 2842 2923 3258 5402 2842
Size: 81 335 2144 3070
Fragments arranged by size:
3070 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3997 3997
Size: 5630
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1148 1232 1386 1400 1568 1652 1736 1820 1904
Size: 84 154 14 168 84 84 84 84
Cuts at: 1904 1988 2156 2240 2408 1148
Size: 84 168 84 168 4370
Fragments arranged by size:
4370 168 168 168 154 84 84 84
84 84 84 84 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 2806 3685 4177 4475 550
Size: 2256 879 492 298 1705
Fragments arranged by size:
2256 1705 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5334 5334
Size: 5630
BsaAI yAC'GTr
Cuts at: 2983 2983
Size: 5630
BsaBI GATnn'nnATC
Cuts at: 6 3144 5181 6
Size: 3138 2037 455
Fragments arranged by size:
3138 2037 455
BsaHI Gr'CG_yC
Cuts at: 3061 3309 4011 5507 3061
Size: 248 702 1496 3184
Fragments arranged by size:
3184 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1462 2470 2819 3340 4018 4292 4439 4850 1462
Size: 1008 349 521 678 274 147 411 2242
Fragments arranged by size:
2242 1008 678 521 411 349 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 226 256 226
Size: 30 5600
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 181
Size: 5630
BseYI C'CCAG_C
Cuts at: 3055 3813 4390 4886 3055
Size: 758 577 496 3799
Fragments arranged by size:
3799 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3172 3218 4426 104
Size: 3068 46 1208 1308
Fragments arranged by size:
3068 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1260 1596 2644 3422 3612 4404 1260
Size: 336 1048 778 190 792 2486
Fragments arranged by size:
2486 1048 792 778 336 190
BsmI GAATG_Cn'
Cuts at: 1495 1495
Size: 5630
Bsp1286I G_dGCh'C
Cuts at: 1260 1596 2644 3255 3348 3422 3612 3674 4404
Size: 336 1048 611 93 74 190 62 730
Cuts at: 4404 1260
Size: 2486
Fragments arranged by size:
2486 1048 730 611 336 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4850 4850
Size: 5630
BspHI T'CATG_A
Cuts at: 1485 4806 1485
Size: 3321 2309
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2680 2894 3196 3577 2680
Size: 214 302 381 4733
Fragments arranged by size:
4733 381 302 214
BsrBI CCG'CTC
Cuts at: 50 190 3922 3976 5460 5625 50
Size: 140 3732 54 1484 165 55
Fragments arranged by size:
3732 1484 165 140 55 54
BsrDI GCAATG_nn'
Cuts at: 2331 3542 2331
Size: 1211 4419
BsrFI r'CCGG_y
Cuts at: 3628 3809 4018 3628
Size: 181 209 5240
Fragments arranged by size:
5240 209 181
BsrGI T'GTAC_A
Cuts at: 203 203
Size: 5630
BssSI C'ACGA_G
Cuts at: 3901 4259 3901
Size: 358 5272
BstAPI GCAn_nnn'nTGC
Cuts at: 3007 3007
Size: 5630
BstBI TT'CG_AA
Cuts at: 3991 3991
Size: 5630
BstXI CCAn_nnnn'nTGG
Cuts at: 350 2548 350
Size: 2198 3432
BstYI r'GATC_y
Cuts at: 1 2655 3122 3145 3480 3726 4738 4824 4836
Size: 2654 467 23 335 246 1012 86 12
Cuts at: 4836 5182 1
Size: 346 449
Fragments arranged by size:
2654 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5630
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3580 3761 3924 3580
Size: 181 163 5286
Fragments arranged by size:
5286 181 163
BtsI GCAGTG_nn'
Cuts at: 2873 2873
Size: 5630
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2313 2348 2313
Size: 35 5595
DraI TTT'AAA
Cuts at: 2629 2629
Size: 5630
DrdI GACnn_nn'nnGTC
Cuts at: 3336 4194 3336
Size: 858 4772
EaeI y'GGCC_r
Cuts at: 3215 3389 3780 3807 5152 3215
Size: 174 391 27 1345 3693
Fragments arranged by size:
3693 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3215 3215
Size: 5630
EarI CTCTTCn'nnn_
Cuts at: 390 418 600 843 3653 3863 390
Size: 28 182 243 2810 210 2157
Fragments arranged by size:
2810 2157 243 210 182 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3838 4148 4294 5440 3838
Size: 310 146 1146 4028
Fragments arranged by size:
4028 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1258 2642 1258
Size: 1384 4246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 483 572 655 1148 1232 1386 1400 1568 1652
Size: 89 83 493 84 154 14 168 84
Cuts at: 1652 1736 1740 1820 1904 1988 2156 2240 2408
Size: 84 4 80 84 84 168 84 168
Cuts at: 2408 3455 3887 4634 4902 483
Size: 1047 432 747 268 1211
Fragments arranged by size:
1211 1047 747 493 432 268 168 168
168 154 89 84 84 84 84 84
84 83 80 14 4
EcoO109I rG'GnC_Cy
Cuts at: 2769 2769
Size: 5630
EcoRI G'AATT_C
Cuts at: 154 1176 1202 1370 1706 1848 1874 1958 2100
Size: 1022 26 168 336 142 26 84 142
Cuts at: 2100 2352 2378 2462 2619 2634 154
Size: 252 26 84 157 15 3150
Fragments arranged by size:
3150 1022 336 252 168 157 142 142
84 84 26 26 26 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 589 3649 589
Size: 3060 2570
FspI TGC'GCA
Cuts at: 3411 3411
Size: 5630
HaeII r_GCGC'y
Cuts at: 3064 3312 4334 5313 3064
Size: 248 1022 979 3381
Fragments arranged by size:
3381 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 363 395 820 852 3472 3502 3504 3534 363
Size: 32 425 32 2620 30 2 30 2459
Fragments arranged by size:
2620 2459 425 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2669 2669
Size: 5630
HindIII A'AGCT_T
Cuts at: 640 1266 2191 640
Size: 626 925 4079
Fragments arranged by size:
4079 925 626
KasI G'GCGC_C
Cuts at: 3060 3308 3060
Size: 248 5382
KpnI G_GTAC'C
Cuts at: 2650 2650
Size: 5630
MluI A'CGCG_T
Cuts at: 5063 5063
Size: 5630
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 200 266 440 4301 4485 200
Size: 66 174 3861 184 1345
Fragments arranged by size:
3861 1345 184 174 66
MscI TGG'CCA
Cuts at: 3391 3391
Size: 5630
MslI CAynn'nnrTG
Cuts at: 2051 2235 2487 2546 3619 3746 2051
Size: 184 252 59 1073 127 3935
Fragments arranged by size:
3935 1073 252 184 127 59
MspA1I CmG'CkG
Cuts at: 2741 3055 3415 4428 4673 2741
Size: 314 360 1013 245 3698
Fragments arranged by size:
3698 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3811 3811
Size: 5630
NarI GG'CG_CC
Cuts at: 3061 3309 3061
Size: 248 5382
NcoI C'CATG_G
Cuts at: 107 107
Size: 5630
NdeI CA'TA_TG
Cuts at: 2232 2484 2232
Size: 252 5378
NgoMIV G'CCGG_C
Cuts at: 3809 3809
Size: 5630
NspI r_CATG'y
Cuts at: 179 2683 3183 4090 179
Size: 2504 500 907 1719
Fragments arranged by size:
2504 1719 907 500
PasI CC'CwG_GG
Cuts at: 120 246 120
Size: 126 5504
PciI A'CATG_T
Cuts at: 4086 4086
Size: 5630
PmeI GTTT'AAAC
Cuts at: 2629 2629
Size: 5630
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 226 258 4769 4801 226
Size: 32 4511 32 1055
Fragments arranged by size:
4511 1055 32 32
PsiI TTA'TAA
Cuts at: 791 1142 1562 1898 791
Size: 351 420 336 4523
Fragments arranged by size:
4523 420 351 336
PstI C_TGCA'G
Cuts at: 695 2677 3362 695
Size: 1982 685 2963
Fragments arranged by size:
2963 1982 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5630
PvuII CAG'CTG
Cuts at: 3055 3415 3055
Size: 360 5270
RsrII CG'GwC_CG
Cuts at: 3825 3825
Size: 5630
SacI G_AGCT'C
Cuts at: 1260 2644 1260
Size: 1384 4246
SalI G'TCGA_C
Cuts at: 2667 2667
Size: 5630
SapI GCTCTTCn'nnn_
Cuts at: 3653 3863 3653
Size: 210 5420
SbfI CC_TGCA'GG
Cuts at: 2677 2677
Size: 5630
ScaI AGT'ACT
Cuts at: 5147 5147
Size: 5630
SfcI C'TryA_G
Cuts at: 33 149 691 2673 3358 4351 4542 33
Size: 116 542 1982 685 993 191 1121
Fragments arranged by size:
1982 1121 993 685 542 191 116
SfoI GGC'GCC
Cuts at: 3062 3310 3062
Size: 248 5382
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5630
SmaI CCC'GGG
Cuts at: 2652 2652
Size: 5630
SmlI C'TyrA_G
Cuts at: 529 2785 3700 4192 4454 529
Size: 2256 915 492 262 1705
Fragments arranged by size:
2256 1705 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5630
SphI G_CATG'C
Cuts at: 2683 3183 2683
Size: 500 5130
StyI C'CwwG_G
Cuts at: 107 2764 3118 107
Size: 2657 354 2619
Fragments arranged by size:
2657 2619 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 133 3213 3346 3805 4014 133
Size: 3080 133 459 209 1749
Fragments arranged by size:
3080 1749 459 209 133
TatI w'GTAC_w
Cuts at: 203 324 5145 203
Size: 121 4821 688
Fragments arranged by size:
4821 688 121
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5147 5179 5147
Size: 32 5598
Tth111I GACn'n_nGTC
Cuts at: 3427 3427
Size: 5630
XbaI T'CTAG_A
Cuts at: 2661 2661
Size: 5630
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3125 3125
Size: 5630
XmaI C'CCGG_G
Cuts at: 2650 2650
Size: 5630
XmnI GAAnn'nnTTC
Cuts at: 745 1008 2341 745
Size: 263 1333 4034
Fragments arranged by size:
4034 1333 263
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsgI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I
ClaI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NheI NotI NruI NsiI PacI PflMI PfoI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI