Restriction map for
pF1KB9767
(Circular) (Six-base) MAPSORT of: pF1KB9767.seq Check: 2649 from: 1 to: 5954
pF1KB9767 5954 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 123 2992 123
Size: 2869 3085
Acc65I G'GTAC_C
Cuts at: 2890 2970 2890
Size: 80 5874
AclI AA'CG_TT
Cuts at: 5794 5794
Size: 5954
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 365 677 758 1186 1349 1369 1373 1631
Size: 178 312 81 428 163 20 4 258
Cuts at: 1631 2069 2219 3779 4211 4958 5226 187
Size: 438 150 1560 432 747 268 915
Fragments arranged by size:
1560 915 747 438 432 428 312 268
258 178 163 150 81 20 4
AfeI AGC'GCT
Cuts at: 352 352
Size: 5954
AflII C'TTAA_G
Cuts at: 2076 2076
Size: 5954
AflIII A'CryG_T
Cuts at: 2289 3306 4410 5387 2289
Size: 1017 1104 977 2856
Fragments arranged by size:
2856 1104 1017 977
AgeI A'CCGG_T
Cuts at: 4342 4342
Size: 5954
AleI CACnn'nnGTG
Cuts at: 935 935
Size: 5954
AlwNI CAG_nnn'CTG
Cuts at: 2056 3259 4826 2056
Size: 1203 1567 3184
Fragments arranged by size:
3184 1567 1203
ApaLI G'TGCA_C
Cuts at: 4724 4724
Size: 5954
ApoI r'AATT_y
Cuts at: 635 796 1114 1947 2378 2958 5307 5563 635
Size: 161 318 833 431 580 2349 256 1026
Fragments arranged by size:
2349 1026 833 580 431 318 256 161
AseI AT'TA_AT
Cuts at: 20 1135 20
Size: 1115 4839
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5954
AvaI C'yCGr_G
Cuts at: 2974 2974
Size: 5954
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 190 223 190
Size: 33 5921
BamHI G'GATC_C
Cuts at: 2979 2979
Size: 5954
BanI G'GyrC_C
Cuts at: 2890 2970 3384 3632 3667 2890
Size: 80 414 248 35 5177
Fragments arranged by size:
5177 414 248 80 35
BanII G_rGCy'C
Cuts at: 2582 2968 3998 2582
Size: 386 1030 4538
Fragments arranged by size:
4538 1030 386
BbeI G_GCGC'C
Cuts at: 3388 3636 3388
Size: 248 5706
BbsI GAAGACnn'nnnn_
Cuts at: 401 907 401
Size: 506 5448
BciVI GTATCCnnnnn_n'
Cuts at: 3852 4613 5954 3852
Size: 761 1341 3852
Fragments arranged by size:
3852 1341 761
BclI T'GATC_A
Cuts at: 3474 3474
Size: 5954
BfrBI ATG'CAT
Cuts at: 982 1108 2189 2433 982
Size: 126 1081 244 4503
Fragments arranged by size:
4503 1081 244 126
BglII A'GATC_T
Cuts at: 3469 5506 3469
Size: 2037 3917
BlpI GC'TnA_GC
Cuts at: 3066 3066
Size: 5954
Bme1580I G_kGCm'C
Cuts at: 3579 3672 4728 3579
Size: 93 1056 4805
Fragments arranged by size:
4805 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 3166 3247 3582 5726 3166
Size: 81 335 2144 3394
Fragments arranged by size:
3394 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4321 4321
Size: 5954
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 687 1151 1402 1706 2783 461
Size: 226 464 251 304 1077 3632
Fragments arranged by size:
3632 1077 464 304 251 226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2672 2786 3130 4009 4501 4799 2672
Size: 114 344 879 492 298 3827
Fragments arranged by size:
3827 879 492 344 298 114
BsaI GGTCTCn'nnnn_
Cuts at: 1833 5658 1833
Size: 3825 2129
BsaAI yAC'GTr
Cuts at: 3307 3307
Size: 5954
BsaBI GATnn'nnATC
Cuts at: 6 3468 5505 6
Size: 3462 2037 455
Fragments arranged by size:
3462 2037 455
BsaHI Gr'CG_yC
Cuts at: 2213 3385 3633 4335 5831 2213
Size: 1172 248 702 1496 2336
Fragments arranged by size:
2336 1496 1172 702 248
BsaWI w'CCGG_w
Cuts at: 3143 3664 4342 4616 4763 5174 3143
Size: 521 678 274 147 411 3923
Fragments arranged by size:
3923 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 680 710 747 777 680
Size: 30 37 30 5857
Fragments arranged by size:
5857 37 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 140 1314 2880 2883 2886 140
Size: 1174 1566 3 3 3208
Fragments arranged by size:
3208 1566 1174 3 3
BseYI C'CCAG_C
Cuts at: 601 3379 4137 4714 5210 601
Size: 2778 758 577 496 1345
Fragments arranged by size:
2778 1345 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3496 3542 4750 104
Size: 3392 46 1208 1308
Fragments arranged by size:
3392 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2582 2761 2968 3746 3936 4728 2582
Size: 179 207 778 190 792 3808
Fragments arranged by size:
3808 792 778 207 190 179
BsmI GAATG_Cn'
Cuts at: 1202 2191 2517 1202
Size: 989 326 4639
Fragments arranged by size:
4639 989 326
Bsp1286I G_dGCh'C
Cuts at: 2582 2761 2968 3579 3672 3746 3936 3998 4728
Size: 179 207 611 93 74 190 62 730
Cuts at: 4728 2582
Size: 3808
Fragments arranged by size:
3808 730 611 207 190 179 93 74
62
BspEI T'CCGG_A
Cuts at: 5174 5174
Size: 5954
BspHI T'CATG_A
Cuts at: 1142 5130 1142
Size: 3988 1966
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3004 3218 3520 3901 3004
Size: 214 302 381 5057
Fragments arranged by size:
5057 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4246 4300 5784 5949 50
Size: 4196 54 1484 165 55
Fragments arranged by size:
4196 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1515 2888 3866 1515
Size: 1373 978 3603
Fragments arranged by size:
3603 1373 978
BsrFI r'CCGG_y
Cuts at: 3952 4133 4342 3952
Size: 181 209 5564
Fragments arranged by size:
5564 209 181
BsrGI T'GTAC_A
Cuts at: 1404 2487 1404
Size: 1083 4871
BssSI C'ACGA_G
Cuts at: 4225 4583 4225
Size: 358 5596
BstAPI GCAn_nnn'nTGC
Cuts at: 3331 3331
Size: 5954
BstBI TT'CG_AA
Cuts at: 1214 4315 1214
Size: 3101 2853
BstYI r'GATC_y
Cuts at: 1 1059 1232 2703 2979 3446 3469 3804 4050
Size: 1058 173 1471 276 467 23 335 246
Cuts at: 4050 5062 5148 5160 5506 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1471 1058 1012 467 449 346 335 276
246 173 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2391 2391
Size: 5954
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3904 4085 4248 3904
Size: 181 163 5610
Fragments arranged by size:
5610 181 163
BtsI GCAGTG_nn'
Cuts at: 829 2076 2100 3197 829
Size: 1247 24 1097 3586
Fragments arranged by size:
3586 1247 1097 24
DraI TTT'AAA
Cuts at: 335 2953 335
Size: 2618 3336
DrdI GACnn_nn'nnGTC
Cuts at: 3660 4518 3660
Size: 858 5096
EaeI y'GGCC_r
Cuts at: 3539 3713 4104 4131 5476 3539
Size: 174 391 27 1345 4017
Fragments arranged by size:
4017 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3539 3539
Size: 5954
EarI CTCTTCn'nnn_
Cuts at: 509 736 1344 2088 2587 2762 3977 4187 509
Size: 227 608 744 499 175 1215 210 2276
Fragments arranged by size:
2276 1215 744 608 499 227 210 175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4162 4472 4618 5764 4162
Size: 310 146 1146 4352
Fragments arranged by size:
4352 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2580 2966 2580
Size: 386 5568
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 365 461 677 687 758 1151 1186 1349
Size: 178 96 216 10 71 393 35 163
Cuts at: 1349 1369 1373 1402 1631 1706 2069 2219 2783
Size: 20 4 29 229 75 363 150 564
Cuts at: 2783 3779 4211 4958 5226 187
Size: 996 432 747 268 915
Fragments arranged by size:
996 915 747 564 432 393 363 268
229 216 178 163 150 96 75 71
35 29 20 10 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2390 2390
Size: 5954
EcoO109I rG'GnC_Cy
Cuts at: 3093 3093
Size: 5954
EcoRI G'AATT_C
Cuts at: 2958 2958
Size: 5954
EcoRV GAT'ATC
Cuts at: 1284 1284
Size: 5954
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 369 973 1748 1903 3973 369
Size: 604 775 155 2070 2350
Fragments arranged by size:
2350 2070 775 604 155
FspI TGC'GCA
Cuts at: 3735 3735
Size: 5954
HaeII r_GCGC'y
Cuts at: 354 3388 3636 4658 5637 354
Size: 3034 248 1022 979 671
Fragments arranged by size:
3034 1022 979 671 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 680 712 2065 2089 2097 2121 2233 2265 3796
Size: 32 1353 24 8 24 112 32 1531
Cuts at: 3796 3826 3828 3858 680
Size: 30 2 30 2776
Fragments arranged by size:
2776 1531 1353 112 32 32 30 30
24 24 8 2
HincII GTy'rAC
Cuts at: 124 144 699 1005 2993 124
Size: 20 555 306 1988 3085
Fragments arranged by size:
3085 1988 555 306 20
HindIII A'AGCT_T
Cuts at: 1381 1381
Size: 5954
HpaI GTT'AAC
Cuts at: 144 144
Size: 5954
KasI G'GCGC_C
Cuts at: 3384 3632 3384
Size: 248 5706
KpnI G_GTAC'C
Cuts at: 2894 2974 2894
Size: 80 5874
MluI A'CGCG_T
Cuts at: 5387 5387
Size: 5954
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 829 4625 4809 829
Size: 3796 184 1974
Fragments arranged by size:
3796 1974 184
MscI TGG'CCA
Cuts at: 3715 3715
Size: 5954
MslI CAynn'nnrTG
Cuts at: 935 981 2425 2458 2899 3943 4070 935
Size: 46 1444 33 441 1044 127 2819
Fragments arranged by size:
2819 1444 1044 441 127 46 33
MspA1I CmG'CkG
Cuts at: 3065 3379 3739 4752 4997 3065
Size: 314 360 1013 245 4022
Fragments arranged by size:
4022 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4135 4135
Size: 5954
NarI GG'CG_CC
Cuts at: 3385 3633 3385
Size: 248 5706
NdeI CA'TA_TG
Cuts at: 978 978
Size: 5954
NgoMIV G'CCGG_C
Cuts at: 4133 4133
Size: 5954
NsiI A_TGCA'T
Cuts at: 984 1110 2191 2435 984
Size: 126 1081 244 4503
Fragments arranged by size:
4503 1081 244 126
NspI r_CATG'y
Cuts at: 1829 2293 3007 3507 4414 1829
Size: 464 714 500 907 3369
Fragments arranged by size:
3369 907 714 500 464
PciI A'CATG_T
Cuts at: 2289 4410 2289
Size: 2121 3833
PflMI CCAn_nnn'nTGG
Cuts at: 2819 2819
Size: 5954
PmeI GTTT'AAAC
Cuts at: 2953 2953
Size: 5954
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5093 5125 5093
Size: 32 5922
PsiI TTA'TAA
Cuts at: 316 995 316
Size: 679 5275
PstI C_TGCA'G
Cuts at: 3001 3686 3001
Size: 685 5269
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5954
PvuII CAG'CTG
Cuts at: 3379 3739 3379
Size: 360 5594
RsrII CG'GwC_CG
Cuts at: 4149 4149
Size: 5954
SacI G_AGCT'C
Cuts at: 2582 2968 2582
Size: 386 5568
SalI G'TCGA_C
Cuts at: 122 2991 122
Size: 2869 3085
SapI GCTCTTCn'nnn_
Cuts at: 509 2088 2587 2762 3977 4187 509
Size: 1579 499 175 1215 210 2276
Fragments arranged by size:
2276 1579 1215 499 210 175
SbfI CC_TGCA'GG
Cuts at: 3001 3001
Size: 5954
ScaI AGT'ACT
Cuts at: 5471 5471
Size: 5954
SfcI C'TryA_G
Cuts at: 33 933 2723 2997 3682 4675 4866 33
Size: 900 1790 274 685 993 191 1121
Fragments arranged by size:
1790 1121 993 900 685 274 191
SfoI GGC'GCC
Cuts at: 3386 3634 3386
Size: 248 5706
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5954
SmaI CCC'GGG
Cuts at: 2976 2976
Size: 5954
SmlI C'TyrA_G
Cuts at: 2076 2687 2801 3109 4024 4516 4778 2076
Size: 611 114 308 915 492 262 3252
Fragments arranged by size:
3252 915 611 492 308 262 114
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5954
SphI G_CATG'C
Cuts at: 3007 3507 3007
Size: 500 5454
SspI AAT'ATT
Cuts at: 367 367
Size: 5954
StyI C'CwwG_G
Cuts at: 3088 3442 3088
Size: 354 5600
SwaI ATTT'AAAT
Cuts at: 335 335
Size: 5954
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 517 615 3537 3670 4129 4338 517
Size: 98 2922 133 459 209 2133
Fragments arranged by size:
2922 2133 459 209 133 98
TatI w'GTAC_w
Cuts at: 1157 1404 2487 5469 1157
Size: 247 1083 2982 1642
Fragments arranged by size:
2982 1642 1083 247
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2219 2251 5471 5503 2219
Size: 32 3220 32 2670
Fragments arranged by size:
3220 2670 32 32
Tth111I GACn'n_nGTC
Cuts at: 1042 3751 1042
Size: 2709 3245
XbaI T'CTAG_A
Cuts at: 471 1951 2985 471
Size: 1480 1034 3440
Fragments arranged by size:
3440 1480 1034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3449 3449
Size: 5954
XmaI C'CCGG_G
Cuts at: 2974 2974
Size: 5954
XmnI GAAnn'nnTTC
Cuts at: 1886 1886
Size: 5954
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI
NspI PciI PflMI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AhdI AlfI AloI ApaI AscI AvrII
BbvCI BcgI BglI BmgBI BmtI Bpu10I BsgI BsiWI
BsmBI BssHII BstEII BstXI BstZ17I BtgI ClaI CspCI
DraIII FseI FspAI MfeI NcoI NheI NotI NruI
PacI PasI PfoI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI XhoI ZraI