Restriction map for
pF1KB9769
(Circular) (Six-base) MAPSORT of: pF1KB9769.seq Check: 5252 from: 1 to: 6254
pF1KB9769 6254 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 2687 3292 2687
Size: 605 5649
Acc65I G'GTAC_C
Cuts at: 3270 3270
Size: 6254
AclI AA'CG_TT
Cuts at: 197 1998 6094 197
Size: 1801 4096 357
Fragments arranged by size:
4096 1801 357
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 701 1066 1240 1898 1945 2039 2542 2683 2930
Size: 365 174 658 47 94 503 141 247
Cuts at: 2930 2942 4079 4511 5258 5526 701
Size: 12 1137 432 747 268 1429
Fragments arranged by size:
1429 1137 747 658 503 432 365 268
247 174 141 94 47 12
AflIII A'CryG_T
Cuts at: 1079 3606 4710 5687 1079
Size: 2527 1104 977 1646
Fragments arranged by size:
2527 1646 1104 977
AgeI A'CCGG_T
Cuts at: 4642 4642
Size: 6254
AleI CACnn'nnGTG
Cuts at: 1366 2317 2335 1366
Size: 951 18 5285
Fragments arranged by size:
5285 951 18
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2308 2340 2308
Size: 32 6222
AlwNI CAG_nnn'CTG
Cuts at: 1354 2929 3559 5126 1354
Size: 1575 630 1567 2482
Fragments arranged by size:
2482 1575 1567 630
ApaLI G'TGCA_C
Cuts at: 3178 5024 3178
Size: 1846 4408
ApoI r'AATT_y
Cuts at: 529 694 1171 2451 2494 2766 3258 5607 5863
Size: 165 477 1280 43 272 492 2349 256
Cuts at: 5863 529
Size: 920
Fragments arranged by size:
2349 1280 920 492 477 272 256 165
43
AseI AT'TA_AT
Cuts at: 20 20
Size: 6254
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6254
AvaI C'yCGr_G
Cuts at: 1472 2009 3274 1472
Size: 537 1265 4452
Fragments arranged by size:
4452 1265 537
AvrII C'CTAG_G
Cuts at: 1149 1149
Size: 6254
BamHI G'GATC_C
Cuts at: 3279 3279
Size: 6254
BanI G'GyrC_C
Cuts at: 1926 3270 3684 3932 3967 1926
Size: 1344 414 248 35 4213
Fragments arranged by size:
4213 1344 414 248 35
BanII G_rGCy'C
Cuts at: 408 647 1186 2535 3268 4298 408
Size: 239 539 1349 733 1030 2364
Fragments arranged by size:
2364 1349 1030 733 539 239
BbeI G_GCGC'C
Cuts at: 3688 3936 3688
Size: 248 6006
BbsI GAAGACnn'nnnn_
Cuts at: 678 2833 678
Size: 2155 4099
BciVI GTATCCnnnnn_n'
Cuts at: 4152 4913 6254 4152
Size: 761 1341 4152
Fragments arranged by size:
4152 1341 761
BclI T'GATC_A
Cuts at: 2063 2717 3774 2063
Size: 654 1057 4543
Fragments arranged by size:
4543 1057 654
BfrBI ATG'CAT
Cuts at: 2379 2379
Size: 6254
BglII A'GATC_T
Cuts at: 1691 2097 3769 5806 1691
Size: 406 1672 2037 2139
Fragments arranged by size:
2139 2037 1672 406
BlpI GC'TnA_GC
Cuts at: 2402 3366 2402
Size: 964 5290
Bme1580I G_kGCm'C
Cuts at: 3182 3879 3972 5028 3182
Size: 697 93 1056 4408
Fragments arranged by size:
4408 1056 697 93
BmgBI CAC'GTC
Cuts at: 1080 1101 1080
Size: 21 6233
BmrI ACTGGGnnnn_n'
Cuts at: 3466 3547 3882 6026 3466
Size: 81 335 2144 3694
Fragments arranged by size:
3694 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2410 4621 2410
Size: 2211 4043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 1009 2167 2902 3106 557
Size: 452 1158 735 204 3705
Fragments arranged by size:
3705 1158 735 452 204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 856 977 2263 3430 4309 4801 5099 856
Size: 121 1286 1167 879 492 298 2011
Fragments arranged by size:
2011 1286 1167 879 492 298 121
BsaI GGTCTCn'nnnn_
Cuts at: 5958 5958
Size: 6254
BsaAI yAC'GTr
Cuts at: 3607 3607
Size: 6254
BsaBI GATnn'nnATC
Cuts at: 6 1696 3768 5805 6
Size: 1690 2072 2037 455
Fragments arranged by size:
2072 2037 1690 455
BsaHI Gr'CG_yC
Cuts at: 3685 3933 4635 6131 3685
Size: 248 702 1496 3808
Fragments arranged by size:
3808 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2075 3443 3964 4642 4916 5063 5474 2075
Size: 1368 521 678 274 147 411 2855
Fragments arranged by size:
2855 1368 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2180 2210 2180
Size: 30 6224
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1051 1441 1444 1447 2201 2998 1051
Size: 390 3 3 754 797 4307
Fragments arranged by size:
4307 797 754 390 3 3
BseYI C'CCAG_C
Cuts at: 802 1347 3679 4437 5014 5510 802
Size: 545 2332 758 577 496 1546
Fragments arranged by size:
2332 1546 758 577 545 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 623 2251 2341 3209 623
Size: 1628 90 868 3668
Fragments arranged by size:
3668 1628 868 90
BsiEI CG_ry'CG
Cuts at: 104 3796 3842 5050 104
Size: 3692 46 1208 1308
Fragments arranged by size:
3692 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 647 1536 1842 2535 3182 3268 4046 4236 5028
Size: 889 306 693 647 86 778 190 792
Cuts at: 5028 647
Size: 1873
Fragments arranged by size:
1873 889 792 778 693 647 306 190
86
BsmI GAATG_Cn'
Cuts at: 399 1971 2289 399
Size: 1572 318 4364
Fragments arranged by size:
4364 1572 318
Bsp1286I G_dGCh'C
Cuts at: 408 647 1186 1536 1842 2535 3182 3268 3879
Size: 239 539 350 306 693 647 86 611
Cuts at: 3879 3972 4046 4236 4298 5028 408
Size: 93 74 190 62 730 1634
Fragments arranged by size:
1634 730 693 647 611 539 350 306
239 190 93 86 74 62
BspEI T'CCGG_A
Cuts at: 5474 5474
Size: 6254
BspHI T'CATG_A
Cuts at: 545 2822 5430 545
Size: 2277 2608 1369
Fragments arranged by size:
2608 2277 1369
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2557 3304 3518 3820 4201 2557
Size: 747 214 302 381 4610
Fragments arranged by size:
4610 747 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2646 4546 4600 6084 6249 50
Size: 2596 1900 54 1484 165 55
Fragments arranged by size:
2596 1900 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 393 1739 1757 4166 393
Size: 1346 18 2409 2481
Fragments arranged by size:
2481 2409 1346 18
BsrFI r'CCGG_y
Cuts at: 4252 4433 4642 4252
Size: 181 209 5864
Fragments arranged by size:
5864 209 181
BsrGI T'GTAC_A
Cuts at: 158 2207 158
Size: 2049 4205
BssSI C'ACGA_G
Cuts at: 4525 4883 4525
Size: 358 5896
BstAPI GCAn_nnn'nTGC
Cuts at: 3631 3631
Size: 6254
BstBI TT'CG_AA
Cuts at: 2858 4615 2858
Size: 1757 4497
BstXI CCAn_nnnn'nTGG
Cuts at: 2161 2358 2161
Size: 197 6057
BstYI r'GATC_y
Cuts at: 1 1691 2097 3279 3746 3769 4104 4350 5362
Size: 1690 406 1182 467 23 335 246 1012
Cuts at: 5362 5448 5460 5806 1
Size: 86 12 346 449
Fragments arranged by size:
1690 1182 1012 467 449 406 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1664 2846 2888 1664
Size: 1182 42 5030
Fragments arranged by size:
5030 1182 42
BtgI C'CryG_G
Cuts at: 107 929 1787 2351 3192 3204 107
Size: 822 858 564 841 12 3157
Fragments arranged by size:
3157 858 841 822 564 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 606 4204 4385 4548 606
Size: 3598 181 163 2312
Fragments arranged by size:
3598 2312 181 163
BtsI GCAGTG_nn'
Cuts at: 418 1842 3497 418
Size: 1424 1655 3175
Fragments arranged by size:
3175 1655 1424
ClaI AT'CG_AT
Cuts at: 1700 1700
Size: 6254
DraI TTT'AAA
Cuts at: 2448 3253 2448
Size: 805 5449
DrdI GACnn_nn'nnGTC
Cuts at: 3960 4818 3960
Size: 858 5396
EaeI y'GGCC_r
Cuts at: 335 2596 3839 4013 4404 4431 5776 335
Size: 2261 1243 174 391 27 1345 813
Fragments arranged by size:
2261 1345 1243 813 391 174 27
EagI C'GGCC_G
Cuts at: 3839 3839
Size: 6254
EarI CTCTTCn'nnn_
Cuts at: 145 544 779 1286 1418 1458 1514 1524 2015
Size: 399 235 507 132 40 56 10 491
Cuts at: 2015 2969 2975 3014 3053 3157 4277 4487 145
Size: 954 6 39 39 104 1120 210 1912
Fragments arranged by size:
1912 1120 954 507 491 399 235 210
132 104 56 40 39 39 10 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 186 4462 4772 4918 6064 186
Size: 4276 310 146 1146 376
Fragments arranged by size:
4276 1146 376 310 146
EcoICRI GAG'CTC
Cuts at: 645 2533 3266 645
Size: 1888 733 3633
Fragments arranged by size:
3633 1888 733
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 701 1009 1066 1240 1898 1945 2039 2167
Size: 144 308 57 174 658 47 94 128
Cuts at: 2167 2542 2683 2902 2930 2942 3106 4079 4511
Size: 375 141 219 28 12 164 973 432
Cuts at: 4511 5258 5526 557
Size: 747 268 1285
Fragments arranged by size:
1285 973 747 658 432 375 308 268
219 174 164 144 141 128 94 57
47 28 12
EcoO109I rG'GnC_Cy
Cuts at: 1452 3393 1452
Size: 1941 4313
EcoRI G'AATT_C
Cuts at: 1171 2766 3258 1171
Size: 1595 492 4167
Fragments arranged by size:
4167 1595 492
EcoRV GAT'ATC
Cuts at: 597 597
Size: 6254
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 474 4273 474
Size: 3799 2455
FspI TGC'GCA
Cuts at: 4035 4035
Size: 6254
HaeII r_GCGC'y
Cuts at: 3688 3936 4958 5937 3688
Size: 248 1022 979 4005
Fragments arranged by size:
4005 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 969 1001 2911 2937 2943 2969 2992 3024 4096
Size: 32 1910 26 6 26 23 32 1072
Cuts at: 4096 4126 4128 4158 969
Size: 30 2 30 3065
Fragments arranged by size:
3065 1910 1072 32 32 30 30 26
26 23 6 2
HincII GTy'rAC
Cuts at: 3293 3293
Size: 6254
KasI G'GCGC_C
Cuts at: 3684 3932 3684
Size: 248 6006
KpnI G_GTAC'C
Cuts at: 3274 3274
Size: 6254
MfeI C'AATT_G
Cuts at: 165 165
Size: 6254
MluI A'CGCG_T
Cuts at: 5687 5687
Size: 6254
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 983 4925 5109 983
Size: 3942 184 2128
Fragments arranged by size:
3942 2128 184
MscI TGG'CCA
Cuts at: 337 2598 4015 337
Size: 2261 1417 2576
Fragments arranged by size:
2576 2261 1417
MslI CAynn'nnrTG
Cuts at: 112 331 976 1099 1366 2159 2315 2317 2335
Size: 219 645 123 267 793 156 2 18
Cuts at: 2335 2378 4243 4370 112
Size: 43 1865 127 1996
Fragments arranged by size:
1996 1865 793 645 267 219 156 127
123 43 18 2
MspA1I CmG'CkG
Cuts at: 3365 3679 4039 5052 5297 3365
Size: 314 360 1013 245 4322
Fragments arranged by size:
4322 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4435 4435
Size: 6254
NarI GG'CG_CC
Cuts at: 3685 3933 3685
Size: 248 6006
NcoI C'CATG_G
Cuts at: 107 929 107
Size: 822 5432
NdeI CA'TA_TG
Cuts at: 902 2375 902
Size: 1473 4781
NgoMIV G'CCGG_C
Cuts at: 4433 4433
Size: 6254
NsiI A_TGCA'T
Cuts at: 2381 2381
Size: 6254
NspI r_CATG'y
Cuts at: 2227 3307 3807 4714 2227
Size: 1080 500 907 3767
Fragments arranged by size:
3767 1080 907 500
PciI A'CATG_T
Cuts at: 4710 4710
Size: 6254
PfoI T'CCnGG_A
Cuts at: 2879 2879
Size: 6254
PmeI GTTT'AAAC
Cuts at: 3253 3253
Size: 6254
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5393 5425 5393
Size: 32 6222
PpuMI rG'GwC_Cy
Cuts at: 1452 1452
Size: 6254
PshAI GACnn'nnGTC
Cuts at: 1888 1888
Size: 6254
PsiI TTA'TAA
Cuts at: 1013 2003 1013
Size: 990 5264
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1928 1960 1928
Size: 32 6222
PstI C_TGCA'G
Cuts at: 1518 3301 3986 1518
Size: 1783 685 3786
Fragments arranged by size:
3786 1783 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6254
PvuII CAG'CTG
Cuts at: 3679 4039 3679
Size: 360 5894
RsrII CG'GwC_CG
Cuts at: 3108 4449 3108
Size: 1341 4913
SacI G_AGCT'C
Cuts at: 647 2535 3268 647
Size: 1888 733 3633
Fragments arranged by size:
3633 1888 733
SalI G'TCGA_C
Cuts at: 3291 3291
Size: 6254
SapI GCTCTTCn'nnn_
Cuts at: 544 1524 4277 4487 544
Size: 980 2753 210 2311
Fragments arranged by size:
2753 2311 980 210
SbfI CC_TGCA'GG
Cuts at: 3301 3301
Size: 6254
ScaI AGT'ACT
Cuts at: 5771 5771
Size: 6254
SfcI C'TryA_G
Cuts at: 33 1514 2538 2697 2732 3297 3982 4975 5166
Size: 1481 1024 159 35 565 685 993 191
Cuts at: 5166 33
Size: 1121
Fragments arranged by size:
1481 1121 1024 993 685 565 191 159
35
SfoI GGC'GCC
Cuts at: 3686 3934 3686
Size: 248 6006
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6254
SmaI CCC'GGG
Cuts at: 3276 3276
Size: 6254
SmlI C'TyrA_G
Cuts at: 871 992 2009 2278 3409 4324 4816 5078 871
Size: 121 1017 269 1131 915 492 262 2047
Fragments arranged by size:
2047 1131 1017 915 492 269 262 121
SpeI A'CTAG_T
Cuts at: 67 2566 67
Size: 2499 3755
SphI G_CATG'C
Cuts at: 2227 3307 3807 2227
Size: 1080 500 4674
Fragments arranged by size:
4674 1080 500
SspI AAT'ATT
Cuts at: 1920 2118 2217 1920
Size: 198 99 5957
Fragments arranged by size:
5957 198 99
StyI C'CwwG_G
Cuts at: 107 929 1149 3388 3742 107
Size: 822 220 2239 354 2619
Fragments arranged by size:
2619 2239 822 354 220
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 211 2399 3125 3837 3970 4429 4638 211
Size: 2188 726 712 133 459 209 1827
Fragments arranged by size:
2188 1827 726 712 459 209 133
TatI w'GTAC_w
Cuts at: 158 1989 2207 2796 5769 158
Size: 1831 218 589 2973 643
Fragments arranged by size:
2973 1831 643 589 218
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2180 2212 5771 5803 2180
Size: 32 3559 32 2631
Fragments arranged by size:
3559 2631 32 32
Tth111I GACn'n_nGTC
Cuts at: 4051 4051
Size: 6254
XbaI T'CTAG_A
Cuts at: 3285 3285
Size: 6254
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2242 3749 2242
Size: 1507 4747
XhoI C'TCGA_G
Cuts at: 2009 2009
Size: 6254
XmaI C'CCGG_G
Cuts at: 3274 3274
Size: 6254
XmnI GAAnn'nnTTC
Cuts at: 1467 1969 1467
Size: 502 5752
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
BaeI BbvCI BcgI BglI BmtI Bpu10I BsiWI BsmBI
BssHII BstEII BstZ17I CspCI DraIII EcoNI FseI FspAI
HindIII HpaI NheI NotI NruI PacI PasI PflMI
PmlI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI ZraI