Restriction map for
pF1KB9770
(Circular) (Six-base) MAPSORT of: pF1KB9770.seq Check: 4370 from: 1 to: 4499
pF1KB9770 4499 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 625 625
Size: 4499
AccI GT'mk_AC
Cuts at: 772 1537 772
Size: 765 3734
Acc65I G'GTAC_C
Cuts at: 1515 1515
Size: 4499
AclI AA'CG_TT
Cuts at: 4339 4339
Size: 4499
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 351 2324 2756 3109 3771 351
Size: 1973 432 353 662 1079
Fragments arranged by size:
1973 1079 662 432 353
AflIII A'CryG_T
Cuts at: 1851 3651 3932 1851
Size: 1800 281 2418
Fragments arranged by size:
2418 1800 281
AgeI A'CCGG_T
Cuts at: 662 662
Size: 4499
AleI CACnn'nnGTG
Cuts at: 148 943 148
Size: 795 3704
AlwNI CAG_nnn'CTG
Cuts at: 1804 3242 1804
Size: 1438 3061
ApaI G_GGCC'C
Cuts at: 295 328 295
Size: 33 4466
ApaLI G'TGCA_C
Cuts at: 3337 3337
Size: 4499
ApoI r'AATT_y
Cuts at: 1503 3852 4108 1503
Size: 2349 256 1894
Fragments arranged by size:
2349 1894 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4499
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
AvaI C'yCGr_G
Cuts at: 237 1519 237
Size: 1282 3217
BamHI G'GATC_C
Cuts at: 809 962 1076 1524 809
Size: 153 114 448 3784
Fragments arranged by size:
3784 448 153 114
BanI G'GyrC_C
Cuts at: 342 665 1135 1476 1515 1929 2177 2212 342
Size: 323 470 341 39 414 248 35 2629
Fragments arranged by size:
2629 470 414 341 323 248 39 35
BanII G_rGCy'C
Cuts at: 274 295 328 839 1256 1397 1513 2543 274
Size: 21 33 511 417 141 116 1030 2230
Fragments arranged by size:
2230 1030 511 417 141 116 33 21
BbeI G_GCGC'C
Cuts at: 1139 1480 1933 2181 1139
Size: 341 453 248 3457
Fragments arranged by size:
3457 453 341 248
BbvCI CC'TCA_GC
Cuts at: 1282 1282
Size: 4499
BciVI GTATCCnnnnn_n'
Cuts at: 2397 3453 4499 2397
Size: 1056 1046 2397
Fragments arranged by size:
2397 1056 1046
BclI T'GATC_A
Cuts at: 2019 2019
Size: 4499
BglI GCCn_nnn'nGGC
Cuts at: 449 449
Size: 4499
BglII A'GATC_T
Cuts at: 2014 4051 2014
Size: 2037 2462
BlpI GC'TnA_GC
Cuts at: 388 821 1355 1611 388
Size: 433 534 256 3276
Fragments arranged by size:
3276 534 433 256
Bme1580I G_kGCm'C
Cuts at: 295 328 347 2124 2217 3341 295
Size: 33 19 1777 93 1124 1453
Fragments arranged by size:
1777 1453 1124 93 33 19
BmgBI CAC'GTC
Cuts at: 1101 1101
Size: 4499
BmrI ACTGGGnnnn_n'
Cuts at: 1711 1792 2127 4271 1711
Size: 81 335 2144 1939
Fragments arranged by size:
2144 1939 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2866 2866
Size: 4499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 835 1492 835
Size: 657 3842
Bpu10I CC'TnA_GC
Cuts at: 268 1282 268
Size: 1014 3485
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 1675 2554 3268 3566 442
Size: 1233 879 714 298 1375
Fragments arranged by size:
1375 1233 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 841 4203 841
Size: 3362 1137
BsaAI yAC'GTr
Cuts at: 855 1852 855
Size: 997 3502
BsaBI GATnn'nnATC
Cuts at: 6 2013 4050 6
Size: 2007 2037 455
Fragments arranged by size:
2037 2007 455
BsaHI Gr'CG_yC
Cuts at: 452 1136 1477 1930 2178 2880 4376 452
Size: 684 341 453 248 702 1496 575
Fragments arranged by size:
1496 702 684 575 453 341 248
BsaWI w'CCGG_w
Cuts at: 662 749 1106 1688 2209 2887 3298 3445 3719
Size: 87 357 582 521 678 411 147 274
Cuts at: 3719 662
Size: 1442
Fragments arranged by size:
1442 678 582 521 411 357 274 147
87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1153 1183 1228 1258 1153
Size: 30 45 30 4394
Fragments arranged by size:
4394 45 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 147 522 742 147
Size: 375 220 3904
Fragments arranged by size:
3904 375 220
BseYI C'CCAG_C
Cuts at: 383 880 1206 1924 2682 3347 3755 383
Size: 497 326 718 758 665 408 1127
Fragments arranged by size:
1127 758 718 665 497 408 326
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 742 1081 190
Size: 552 339 3608
Fragments arranged by size:
3608 552 339
BsiEI CG_ry'CG
Cuts at: 104 1268 2041 2087 3317 104
Size: 1164 773 46 1230 1286
Fragments arranged by size:
1286 1230 1164 773 46
BsiHKAI G_wGCw'C
Cuts at: 274 1513 2291 2481 3341 274
Size: 1239 778 190 860 1432
Fragments arranged by size:
1432 1239 860 778 190
BsmI GAATG_Cn'
Cuts at: 249 249
Size: 4499
Bsp1286I G_dGCh'C
Cuts at: 274 295 328 347 839 1256 1397 1513 2124
Size: 21 33 19 492 417 141 116 611
Cuts at: 2124 2217 2291 2481 2543 3341 274
Size: 93 74 190 62 798 1432
Fragments arranged by size:
1432 798 611 492 417 190 141 116
93 74 62 33 21 19
BspHI T'CATG_A
Cuts at: 2931 2931
Size: 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 625 1549 1763 2065 2446 625
Size: 924 214 302 381 2678
Fragments arranged by size:
2678 924 381 302 214
BsrBI CCG'CTC
Cuts at: 50 132 530 732 1055 2791 2845 4329 4494
Size: 82 398 202 323 1736 54 1484 165
Cuts at: 4494 50
Size: 55
Fragments arranged by size:
1736 1484 398 323 202 165 82 55
54
BsrDI GCAATG_nn'
Cuts at: 2411 2411
Size: 4499
BsrFI r'CCGG_y
Cuts at: 662 798 1374 2497 2678 662
Size: 136 576 1123 181 2483
Fragments arranged by size:
2483 1123 576 181 136
BsrGI T'GTAC_A
Cuts at: 554 554
Size: 4499
BssHII G'CGCG_C
Cuts at: 1218 1218
Size: 4499
BssSI C'ACGA_G
Cuts at: 2770 3478 2770
Size: 708 3791
BstAPI GCAn_nnn'nTGC
Cuts at: 1876 1876
Size: 4499
BstBI TT'CG_AA
Cuts at: 2860 2860
Size: 4499
BstYI r'GATC_y
Cuts at: 1 809 962 1076 1524 1991 2014 2349 2595
Size: 808 153 114 448 467 23 335 246
Cuts at: 2595 2901 2913 2999 4051 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 808 467 449 448 335 306 246
153 114 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 477 534 477
Size: 57 4442
BtgI C'CryG_G
Cuts at: 107 188 320 698 830 107
Size: 81 132 378 132 3776
Fragments arranged by size:
3776 378 132 132 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1020 1044 2449 2630 2793 1020
Size: 24 1405 181 163 2726
Fragments arranged by size:
2726 1405 181 163 24
BtsI GCAGTG_nn'
Cuts at: 1742 1742
Size: 4499
DraI TTT'AAA
Cuts at: 1498 1498
Size: 4499
DrdI GACnn_nn'nnGTC
Cuts at: 997 2205 3549 997
Size: 1208 1344 1947
Fragments arranged by size:
1947 1344 1208
EaeI y'GGCC_r
Cuts at: 695 827 1085 1265 2084 2258 2649 2676 4021
Size: 132 258 180 819 174 391 27 1345
Cuts at: 4021 695
Size: 1173
Fragments arranged by size:
1345 1173 819 391 258 180 174 132
27
EagI C'GGCC_G
Cuts at: 1265 2084 1265
Size: 819 3680
EarI CTCTTCn'nnn_
Cuts at: 1420 2522 2732 1420
Size: 1102 210 3187
Fragments arranged by size:
3187 1102 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1357 2707 3449 3595 4309 1357
Size: 1350 742 146 714 1547
Fragments arranged by size:
1547 1350 742 714 146
EcoICRI GAG'CTC
Cuts at: 272 1511 272
Size: 1239 3260
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 351 835 1492 2324 2756 3109 3771 351
Size: 484 657 832 432 353 662 1079
Fragments arranged by size:
1079 832 662 657 484 432 353
EcoO109I rG'GnC_Cy
Cuts at: 220 325 462 603 1638 220
Size: 105 137 141 1035 3081
Fragments arranged by size:
3081 1035 141 137 105
EcoRI G'AATT_C
Cuts at: 1503 1503
Size: 4499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 597 2518 597
Size: 1921 2578
FspI TGC'GCA
Cuts at: 598 2280 598
Size: 1682 2817
HaeII r_GCGC'y
Cuts at: 785 1118 1139 1282 1480 1933 2181 3411 4182
Size: 333 21 143 198 453 248 1230 771
Cuts at: 4182 785
Size: 1102
Fragments arranged by size:
1230 1102 771 453 333 248 198 143
21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 517 549 754 786 1153 1185 2341 2371 2373
Size: 32 205 32 367 32 1156 30 2
Cuts at: 2373 2403 517
Size: 30 2613
Fragments arranged by size:
2613 1156 367 205 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 773 1538 773
Size: 765 3734
KasI G'GCGC_C
Cuts at: 1135 1476 1929 2177 1135
Size: 341 453 248 3457
Fragments arranged by size:
3457 453 341 248
KpnI G_GTAC'C
Cuts at: 1519 1519
Size: 4499
MluI A'CGCG_T
Cuts at: 3932 3932
Size: 4499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3258 3442 3258
Size: 184 4315
MscI TGG'CCA
Cuts at: 697 1087 2260 697
Size: 390 1173 2936
Fragments arranged by size:
2936 1173 390
MslI CAynn'nnrTG
Cuts at: 148 340 703 799 943 1063 1189 2488 2615
Size: 192 363 96 144 120 126 1299 127
Cuts at: 2615 148
Size: 2032
Fragments arranged by size:
2032 1299 363 192 144 127 126 120
96
MspA1I CmG'CkG
Cuts at: 246 560 657 1610 1924 2284 3068 3313 246
Size: 314 97 953 314 360 784 245 1432
Fragments arranged by size:
1432 953 784 360 314 314 245 97
NaeI GCC'GGC
Cuts at: 1376 2680 1376
Size: 1304 3195
NarI GG'CG_CC
Cuts at: 1136 1477 1930 2178 1136
Size: 341 453 248 3457
Fragments arranged by size:
3457 453 341 248
NcoI C'CATG_G
Cuts at: 107 188 320 698 107
Size: 81 132 378 3908
Fragments arranged by size:
3908 378 132 81
NgoMIV G'CCGG_C
Cuts at: 1374 2678 1374
Size: 1304 3195
NspI r_CATG'y
Cuts at: 798 1188 1552 2052 3655 798
Size: 390 364 500 1603 1642
Fragments arranged by size:
1642 1603 500 390 364
PasI CC'CwG_GG
Cuts at: 216 360 438 216
Size: 144 78 4277
Fragments arranged by size:
4277 144 78
PciI A'CATG_T
Cuts at: 3651 3651
Size: 4499
PfoI T'CCnGG_A
Cuts at: 812 812
Size: 4499
PmeI GTTT'AAAC
Cuts at: 1498 1498
Size: 4499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 868 900 2945 2977 868
Size: 32 2045 32 2390
Fragments arranged by size:
2390 2045 32 32
PpuMI rG'GwC_Cy
Cuts at: 462 603 462
Size: 141 4358
PspOMI G'GGCC_C
Cuts at: 291 324 291
Size: 33 4466
PstI C_TGCA'G
Cuts at: 1546 2231 1546
Size: 685 3814
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4499
PvuII CAG'CTG
Cuts at: 246 560 1924 2284 246
Size: 314 1364 360 2461
Fragments arranged by size:
2461 1364 360 314
RsrII CG'GwC_CG
Cuts at: 2694 2694
Size: 4499
SacI G_AGCT'C
Cuts at: 274 1513 274
Size: 1239 3260
SalI G'TCGA_C
Cuts at: 771 1536 771
Size: 765 3734
SapI GCTCTTCn'nnn_
Cuts at: 2522 2732 2522
Size: 210 4289
SbfI CC_TGCA'GG
Cuts at: 1546 1546
Size: 4499
ScaI AGT'ACT
Cuts at: 673 4016 673
Size: 3343 1156
SexAI A'CCwGG_T
Cuts at: 995 995
Size: 4499
SfcI C'TryA_G
Cuts at: 33 1542 2227 3195 3386 33
Size: 1509 685 968 191 1146
Fragments arranged by size:
1509 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1137 1478 1931 2179 1137
Size: 341 453 248 3457
Fragments arranged by size:
3457 453 341 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
SmaI CCC'GGG
Cuts at: 239 1521 239
Size: 1282 3217
SmlI C'TyrA_G
Cuts at: 457 1654 2569 3283 3545 457
Size: 1197 915 714 262 1411
Fragments arranged by size:
1411 1197 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4499
SphI G_CATG'C
Cuts at: 1552 2052 1552
Size: 500 3999
StuI AGG'CCT
Cuts at: 1312 1312
Size: 4499
StyI C'CwwG_G
Cuts at: 107 188 320 698 1247 1304 1633 1987 107
Size: 81 132 378 549 57 329 354 2619
Fragments arranged by size:
2619 549 378 354 329 132 81 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 160 294 369 859 1458 2082 2215 2674 2883
Size: 134 75 490 599 624 133 459 209
Cuts at: 2883 160
Size: 1776
Fragments arranged by size:
1776 624 599 490 459 209 134 133
75
TatI w'GTAC_w
Cuts at: 554 671 4014 554
Size: 117 3343 1039
Fragments arranged by size:
3343 1039 117
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4016 4048 4016
Size: 32 4467
Tth111I GACn'n_nGTC
Cuts at: 898 997 2296 898
Size: 99 1299 3101
Fragments arranged by size:
3101 1299 99
XbaI T'CTAG_A
Cuts at: 1530 1530
Size: 4499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 285 357 1994 285
Size: 72 1637 2790
Fragments arranged by size:
2790 1637 72
XmaI C'CCGG_G
Cuts at: 237 1519 237
Size: 1282 3217
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmtI BsiWI BsmBI BspEI
BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI