Restriction map for
pF1KB9771
(Circular) (Six-base) MAPSORT of: pF1KB9771.seq Check: 1943 from: 1 to: 4727
pF1KB9771 4727 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 1765 1765
Size: 4727
Acc65I G'GTAC_C
Cuts at: 1743 1743
Size: 4727
AclI AA'CG_TT
Cuts at: 569 4567 569
Size: 3998 729
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 657 2552 2984 3337 3999 657
Size: 1895 432 353 662 1385
Fragments arranged by size:
1895 1385 662 432 353
AflIII A'CryG_T
Cuts at: 531 802 2079 3879 4160 531
Size: 271 1277 1800 281 1098
Fragments arranged by size:
1800 1277 1098 281 271
AleI CACnn'nnGTG
Cuts at: 1243 1243
Size: 4727
AlwNI CAG_nnn'CTG
Cuts at: 916 1207 2032 3470 916
Size: 291 825 1438 2173
Fragments arranged by size:
2173 1438 825 291
ApaLI G'TGCA_C
Cuts at: 296 3565 296
Size: 3269 1458
ApoI r'AATT_y
Cuts at: 1731 4080 4336 1731
Size: 2349 256 2122
Fragments arranged by size:
2349 2122 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4727
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
AvaI C'yCGr_G
Cuts at: 115 1747 115
Size: 1632 3095
BamHI G'GATC_C
Cuts at: 1752 1752
Size: 4727
BanI G'GyrC_C
Cuts at: 1743 2157 2405 2440 1743
Size: 414 248 35 4030
Fragments arranged by size:
4030 414 248 35
BanII G_rGCy'C
Cuts at: 1741 2771 1741
Size: 1030 3697
BbeI G_GCGC'C
Cuts at: 2161 2409 2161
Size: 248 4479
BbsI GAAGACnn'nnnn_
Cuts at: 1340 1340
Size: 4727
BbvCI CC'TCA_GC
Cuts at: 1428 1428
Size: 4727
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1006 1040 1006
Size: 34 4693
BciVI GTATCCnnnnn_n'
Cuts at: 2625 3681 4727 2625
Size: 1056 1046 2625
Fragments arranged by size:
2625 1056 1046
BclI T'GATC_A
Cuts at: 2247 2247
Size: 4727
BglII A'GATC_T
Cuts at: 2242 4279 2242
Size: 2037 2690
BlpI GC'TnA_GC
Cuts at: 1839 1839
Size: 4727
Bme1580I G_kGCm'C
Cuts at: 217 300 1074 2352 2445 3569 217
Size: 83 774 1278 93 1124 1375
Fragments arranged by size:
1375 1278 1124 774 93 83
BmrI ACTGGGnnnn_n'
Cuts at: 1528 1939 2020 2355 4499 1528
Size: 411 81 335 2144 1756
Fragments arranged by size:
2144 1756 411 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1012 1015 3094 1012
Size: 3 2079 2645
Fragments arranged by size:
2645 2079 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 348 1113 145
Size: 203 765 3759
Fragments arranged by size:
3759 765 203
Bpu10I CC'TnA_GC
Cuts at: 1193 1428 1193
Size: 235 4492
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1903 2782 3496 3794 1903
Size: 879 714 298 2836
Fragments arranged by size:
2836 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4431 4431
Size: 4727
BsaAI yAC'GTr
Cuts at: 2080 2080
Size: 4727
BsaBI GATnn'nnATC
Cuts at: 6 2241 4278 6
Size: 2235 2037 455
Fragments arranged by size:
2235 2037 455
BsaHI Gr'CG_yC
Cuts at: 2158 2406 3108 4604 2158
Size: 248 702 1496 2281
Fragments arranged by size:
2281 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1365 1916 2437 3115 3526 3673 3947 1365
Size: 551 521 678 411 147 274 2145
Fragments arranged by size:
2145 678 551 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 541 571 541
Size: 30 4697
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 988 1004 1715 988
Size: 16 711 4000
Fragments arranged by size:
4000 711 16
BseYI C'CCAG_C
Cuts at: 1442 2152 2910 3575 3983 1442
Size: 710 758 665 408 2186
Fragments arranged by size:
2186 758 710 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1687 1687
Size: 4727
BsiEI CG_ry'CG
Cuts at: 104 2269 2315 3545 104
Size: 2165 46 1230 1286
Fragments arranged by size:
2165 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 300 1741 2519 2709 3569 300
Size: 1441 778 190 860 1458
Fragments arranged by size:
1458 1441 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 217 300 1074 1741 2352 2445 2519 2709 2771
Size: 83 774 667 611 93 74 190 62
Cuts at: 2771 3569 217
Size: 798 1375
Fragments arranged by size:
1375 798 774 667 611 190 93 83
74 62
BspHI T'CATG_A
Cuts at: 3159 3159
Size: 4727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 901 1777 1991 2293 2674 901
Size: 876 214 302 381 2954
Fragments arranged by size:
2954 876 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3019 3073 4557 4722 50
Size: 2969 54 1484 165 55
Fragments arranged by size:
2969 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 278 1316 2639 278
Size: 1038 1323 2366
Fragments arranged by size:
2366 1323 1038
BsrFI r'CCGG_y
Cuts at: 543 1562 2725 2906 543
Size: 1019 1163 181 2364
Fragments arranged by size:
2364 1163 1019 181
BsrGI T'GTAC_A
Cuts at: 935 935
Size: 4727
BssSI C'ACGA_G
Cuts at: 349 458 478 2998 3706 349
Size: 109 20 2520 708 1370
Fragments arranged by size:
2520 1370 708 109 20
BstAPI GCAn_nnn'nTGC
Cuts at: 840 916 2104 840
Size: 76 1188 3463
Fragments arranged by size:
3463 1188 76
BstBI TT'CG_AA
Cuts at: 3088 3088
Size: 4727
BstXI CCAn_nnnn'nTGG
Cuts at: 1056 1056
Size: 4727
BstYI r'GATC_y
Cuts at: 1 657 1752 2219 2242 2577 2823 3129 3141
Size: 656 1095 467 23 335 246 306 12
Cuts at: 3141 3227 4279 1
Size: 86 1052 449
Fragments arranged by size:
1095 1052 656 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1325 1325
Size: 4727
BtgI C'CryG_G
Cuts at: 865 978 865
Size: 113 4614
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2677 2858 3021 2677
Size: 181 163 4383
Fragments arranged by size:
4383 181 163
BtsI GCAGTG_nn'
Cuts at: 217 1278 1970 217
Size: 1061 692 2974
Fragments arranged by size:
2974 1061 692
DraI TTT'AAA
Cuts at: 1605 1726 1605
Size: 121 4606
DrdI GACnn_nn'nnGTC
Cuts at: 2433 3777 2433
Size: 1344 3383
EaeI y'GGCC_r
Cuts at: 243 2312 2486 2877 2904 4249 243
Size: 2069 174 391 27 1345 721
Fragments arranged by size:
2069 1345 721 391 174 27
EagI C'GGCC_G
Cuts at: 2312 2312
Size: 4727
EarI CTCTTCn'nnn_
Cuts at: 804 943 1274 1411 2750 2960 804
Size: 139 331 137 1339 210 2571
Fragments arranged by size:
2571 1339 331 210 139 137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1687 2935 3677 3823 4537 1687
Size: 1248 742 146 714 1877
Fragments arranged by size:
1877 1248 742 714 146
EcoICRI GAG'CTC
Cuts at: 1739 1739
Size: 4727
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 348 657 1113 2552 2984 3337 3999 145
Size: 203 309 456 1439 432 353 662 873
Fragments arranged by size:
1439 873 662 456 432 353 309 203
EcoNI CCTnn'n_nnAGG
Cuts at: 204 1643 204
Size: 1439 3288
EcoO109I rG'GnC_Cy
Cuts at: 860 1439 1866 860
Size: 579 427 3721
Fragments arranged by size:
3721 579 427
EcoRI G'AATT_C
Cuts at: 1731 1731
Size: 4727
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2746 2746
Size: 4727
FseI GG_CCGG'CC
Cuts at: 1566 1566
Size: 4727
FspI TGC'GCA
Cuts at: 2508 2508
Size: 4727
HaeII r_GCGC'y
Cuts at: 2161 2409 3639 4410 2161
Size: 248 1230 771 2478
Fragments arranged by size:
2478 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 541 573 1525 1557 1691 1723 2569 2599 2601
Size: 32 952 32 134 32 846 30 2
Cuts at: 2601 2631 541
Size: 30 2637
Fragments arranged by size:
2637 952 846 134 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 512 1766 512
Size: 1254 3473
KasI G'GCGC_C
Cuts at: 2157 2405 2157
Size: 248 4479
KpnI G_GTAC'C
Cuts at: 1747 1747
Size: 4727
MfeI C'AATT_G
Cuts at: 716 716
Size: 4727
MluI A'CGCG_T
Cuts at: 4160 4160
Size: 4727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3486 3670 3486
Size: 184 4543
MscI TGG'CCA
Cuts at: 2488 2488
Size: 4727
MslI CAynn'nnrTG
Cuts at: 1243 2716 2843 1243
Size: 1473 127 3127
Fragments arranged by size:
3127 1473 127
MspA1I CmG'CkG
Cuts at: 1838 2152 2512 3296 3541 1838
Size: 314 360 784 245 3024
Fragments arranged by size:
3024 784 360 314 245
NaeI GCC'GGC
Cuts at: 1564 2908 1564
Size: 1344 3383
NarI GG'CG_CC
Cuts at: 2158 2406 2158
Size: 248 4479
NcoI C'CATG_G
Cuts at: 865 978 865
Size: 113 4614
NgoMIV G'CCGG_C
Cuts at: 1562 2906 1562
Size: 1344 3383
NspI r_CATG'y
Cuts at: 535 806 931 1780 2280 3883 535
Size: 271 125 849 500 1603 1379
Fragments arranged by size:
1603 1379 849 500 271 125
PacI TTA_AT'TAA
Cuts at: 752 752
Size: 4727
PciI A'CATG_T
Cuts at: 531 802 3879 531
Size: 271 3077 1379
Fragments arranged by size:
3077 1379 271
PmeI GTTT'AAAC
Cuts at: 1726 1726
Size: 4727
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3173 3205 3173
Size: 32 4695
PpuMI rG'GwC_Cy
Cuts at: 860 1439 860
Size: 579 4148
PspXI vC'TCGA_Gb
Cuts at: 115 115
Size: 4727
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 681 713 681
Size: 32 4695
PstI C_TGCA'G
Cuts at: 1218 1774 2459 1218
Size: 556 685 3486
Fragments arranged by size:
3486 685 556
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4727
PvuII CAG'CTG
Cuts at: 2152 2512 2152
Size: 360 4367
RsrII CG'GwC_CG
Cuts at: 2922 2922
Size: 4727
SacI G_AGCT'C
Cuts at: 1741 1741
Size: 4727
SalI G'TCGA_C
Cuts at: 1764 1764
Size: 4727
SanDI GG'GwC_CC
Cuts at: 1439 1439
Size: 4727
SapI GCTCTTCn'nnn_
Cuts at: 943 1274 2750 2960 943
Size: 331 1476 210 2710
Fragments arranged by size:
2710 1476 331 210
SbfI CC_TGCA'GG
Cuts at: 1774 1774
Size: 4727
ScaI AGT'ACT
Cuts at: 4244 4244
Size: 4727
SexAI A'CCwGG_T
Cuts at: 962 962
Size: 4727
SfcI C'TryA_G
Cuts at: 33 1214 1266 1770 2455 3423 3614 33
Size: 1181 52 504 685 968 191 1146
Fragments arranged by size:
1181 1146 968 685 504 191 52
SfoI GGC'GCC
Cuts at: 2159 2407 2159
Size: 248 4479
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
SmaI CCC'GGG
Cuts at: 1749 1749
Size: 4727
SmlI C'TyrA_G
Cuts at: 115 1882 2797 3511 3773 115
Size: 1767 915 714 262 1069
Fragments arranged by size:
1767 1069 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4727
SphI G_CATG'C
Cuts at: 931 1780 2280 931
Size: 849 500 3378
Fragments arranged by size:
3378 849 500
StyI C'CwwG_G
Cuts at: 865 978 1861 2215 865
Size: 113 883 354 3377
Fragments arranged by size:
3377 883 354 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 447 1510 2310 2443 2902 3111 447
Size: 1063 800 133 459 209 2063
Fragments arranged by size:
2063 1063 800 459 209 133
TatI w'GTAC_w
Cuts at: 935 4242 935
Size: 3307 1420
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4244 4276 4244
Size: 32 4695
Tth111I GACn'n_nGTC
Cuts at: 2524 2524
Size: 4727
XbaI T'CTAG_A
Cuts at: 1758 1758
Size: 4727
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2222 2222
Size: 4727
XhoI C'TCGA_G
Cuts at: 115 115
Size: 4727
XmaI C'CCGG_G
Cuts at: 1747 1747
Size: 4727
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PacI PciI
PmeI PpiI PpuMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BaeI BfrBI BglI BmgBI BmtI
BsiWI BsmI BsmBI BspEI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoRV FspAI HindIII HpaI NdeI NheI
NotI NruI NsiI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI SacII SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XmnI ZraI