Restriction map for
pF1KB9772
(Circular) (Six-base) MAPSORT of: pF1KB9772.seq Check: 9402 from: 1 to: 4760
pF1KB9772 4760 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 446 714 1798 446
Size: 268 1084 3408
Fragments arranged by size:
3408 1084 268
Acc65I G'GTAC_C
Cuts at: 1776 1776
Size: 4760
AclI AA'CG_TT
Cuts at: 489 4600 489
Size: 4111 649
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 250 504 604 1593 1677 2585 3017 3370
Size: 41 254 100 989 84 908 432 353
Cuts at: 3370 4032 209
Size: 662 937
Fragments arranged by size:
989 937 908 662 432 353 254 100
84 41
AflII C'TTAA_G
Cuts at: 385 954 385
Size: 569 4191
AflIII A'CryG_T
Cuts at: 393 1501 1537 2112 3912 4193 393
Size: 1108 36 575 1800 281 960
Fragments arranged by size:
1800 1108 960 575 281 36
AlwNI CAG_nnn'CTG
Cuts at: 2065 3503 2065
Size: 1438 3322
ApaLI G'TGCA_C
Cuts at: 555 3598 555
Size: 3043 1717
ApoI r'AATT_y
Cuts at: 126 1110 1139 1391 1764 4113 4369 126
Size: 984 29 252 373 2349 256 517
Fragments arranged by size:
2349 984 517 373 256 252 29
AseI AT'TA_AT
Cuts at: 20 605 20
Size: 585 4175
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4760
AvaI C'yCGr_G
Cuts at: 1780 1780
Size: 4760
BamHI G'GATC_C
Cuts at: 1071 1559 1785 1071
Size: 488 226 4046
Fragments arranged by size:
4046 488 226
BanI G'GyrC_C
Cuts at: 1776 2190 2438 2473 1776
Size: 414 248 35 4063
Fragments arranged by size:
4063 414 248 35
BanII G_rGCy'C
Cuts at: 1774 2804 1774
Size: 1030 3730
BbeI G_GCGC'C
Cuts at: 2194 2442 2194
Size: 248 4512
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4726
BciVI GTATCCnnnnn_n'
Cuts at: 2658 3714 4760 2658
Size: 1056 1046 2658
Fragments arranged by size:
2658 1056 1046
BclI T'GATC_A
Cuts at: 1128 2280 1128
Size: 1152 3608
BglII A'GATC_T
Cuts at: 1027 2275 4312 1027
Size: 1248 2037 1475
Fragments arranged by size:
2037 1475 1248
BlpI GC'TnA_GC
Cuts at: 1872 1872
Size: 4760
Bme1580I G_kGCm'C
Cuts at: 559 2385 2478 3602 559
Size: 1826 93 1124 1717
Fragments arranged by size:
1826 1717 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 155 1972 2053 2388 4532 155
Size: 1817 81 335 2144 383
Fragments arranged by size:
2144 1817 383 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 309 670 3127 309
Size: 361 2457 1942
Fragments arranged by size:
2457 1942 361
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 279 351 500 833 917 1337 1421 1437
Size: 146 72 149 333 84 420 84 16
Cuts at: 1437 1589 1673 133
Size: 152 84 3220
Fragments arranged by size:
3220 420 333 152 149 146 84 84
84 72 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1936 2815 3529 3827 1936
Size: 879 714 298 2869
Fragments arranged by size:
2869 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4464 4464
Size: 4760
BsaAI yAC'GTr
Cuts at: 1502 1538 2113 1502
Size: 36 575 4149
Fragments arranged by size:
4149 575 36
BsaBI GATnn'nnATC
Cuts at: 6 2274 4311 6
Size: 2268 2037 455
Fragments arranged by size:
2268 2037 455
BsaHI Gr'CG_yC
Cuts at: 2191 2439 3141 4637 2191
Size: 248 702 1496 2314
Fragments arranged by size:
2314 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1949 2470 3148 3559 3706 3980 1949
Size: 521 678 411 147 274 2729
Fragments arranged by size:
2729 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 320 350 320
Size: 30 4730
BseYI C'CCAG_C
Cuts at: 2185 2943 3608 4016 2185
Size: 758 665 408 2929
Fragments arranged by size:
2929 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 445 223
Size: 222 4538
BsiEI CG_ry'CG
Cuts at: 104 2302 2348 3578 104
Size: 2198 46 1230 1286
Fragments arranged by size:
2198 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 559 1774 2552 2742 3602 559
Size: 1215 778 190 860 1717
Fragments arranged by size:
1717 1215 860 778 190
BsmI GAATG_Cn'
Cuts at: 1003 1446 1003
Size: 443 4317
Bsp1286I G_dGCh'C
Cuts at: 559 1774 2385 2478 2552 2742 2804 3602 559
Size: 1215 611 93 74 190 62 798 1717
Fragments arranged by size:
1717 1215 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 1719 3192 1719
Size: 1473 3287
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1810 2024 2326 2707 1810
Size: 214 302 381 3863
Fragments arranged by size:
3863 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3052 3106 4590 4755 50
Size: 3002 54 1484 165 55
Fragments arranged by size:
3002 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 358 2672 358
Size: 2314 2446
BsrFI r'CCGG_y
Cuts at: 2758 2939 2758
Size: 181 4579
BssSI C'ACGA_G
Cuts at: 3031 3739 3031
Size: 708 4052
BstAPI GCAn_nnn'nTGC
Cuts at: 2137 2137
Size: 4760
BstBI TT'CG_AA
Cuts at: 1137 1713 3121 1137
Size: 576 1408 2776
Fragments arranged by size:
2776 1408 576
BstYI r'GATC_y
Cuts at: 1 1027 1071 1279 1559 1785 2252 2275 2610
Size: 1026 44 208 280 226 467 23 335
Cuts at: 2610 2856 3162 3174 3260 4312 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 1026 467 449 335 306 280 246
226 208 86 44 23 12
BstZ17I GTA'TAC
Cuts at: 715 715
Size: 4760
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2710 2891 3054 2710
Size: 181 163 4416
Fragments arranged by size:
4416 181 163
BtsI GCAGTG_nn'
Cuts at: 588 1061 2003 588
Size: 473 942 3345
Fragments arranged by size:
3345 942 473
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 684 719 990 1025 684
Size: 35 271 35 4419
Fragments arranged by size:
4419 271 35 35
DraI TTT'AAA
Cuts at: 1052 1759 1052
Size: 707 4053
DrdI GACnn_nn'nnGTC
Cuts at: 2466 3810 2466
Size: 1344 3416
EaeI y'GGCC_r
Cuts at: 131 2345 2519 2910 2937 4282 131
Size: 2214 174 391 27 1345 609
Fragments arranged by size:
2214 1345 609 391 174 27
EagI C'GGCC_G
Cuts at: 2345 2345
Size: 4760
EarI CTCTTCn'nnn_
Cuts at: 157 182 2783 2993 157
Size: 25 2601 210 1924
Fragments arranged by size:
2601 1924 210 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2968 3710 3856 4570 2968
Size: 742 146 714 3158
Fragments arranged by size:
3158 742 714 146
EcoICRI GAG'CTC
Cuts at: 1772 1772
Size: 4760
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 209 250 279 351 500 504 604 833
Size: 76 41 29 72 149 4 100 229
Cuts at: 833 917 1337 1421 1437 1589 1593 1673 1677
Size: 84 420 84 16 152 4 80 4
Cuts at: 1677 2585 3017 3370 4032 133
Size: 908 432 353 662 861
Fragments arranged by size:
908 861 662 432 420 353 229 152
149 100 84 84 80 76 72 41
29 16 4 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 268 855 268
Size: 587 4173
EcoO109I rG'GnC_Cy
Cuts at: 1241 1899 1241
Size: 658 4102
EcoRI G'AATT_C
Cuts at: 1139 1391 1764 1139
Size: 252 373 4135
Fragments arranged by size:
4135 373 252
EcoRV GAT'ATC
Cuts at: 145 145
Size: 4760
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 259 1092 2779 259
Size: 833 1687 2240
Fragments arranged by size:
2240 1687 833
FspI TGC'GCA
Cuts at: 2541 2541
Size: 4760
HaeII r_GCGC'y
Cuts at: 1487 2194 2442 3672 4443 1487
Size: 707 248 1230 771 1804
Fragments arranged by size:
1804 1230 771 707 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 134 166 320 352 1732 1764 2602 2632 2634
Size: 32 154 32 1380 32 838 30 2
Cuts at: 2634 2664 134
Size: 30 2230
Fragments arranged by size:
2230 1380 838 154 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 868 961 1545 1799 868
Size: 93 584 254 3829
Fragments arranged by size:
3829 584 254 93
HindIII A'AGCT_T
Cuts at: 221 569 221
Size: 348 4412
HpaI GTT'AAC
Cuts at: 1545 1545
Size: 4760
KasI G'GCGC_C
Cuts at: 2190 2438 2190
Size: 248 4512
KpnI G_GTAC'C
Cuts at: 1780 1780
Size: 4760
MluI A'CGCG_T
Cuts at: 4193 4193
Size: 4760
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3519 3703 3519
Size: 184 4576
MscI TGG'CCA
Cuts at: 133 2521 133
Size: 2388 2372
MslI CAynn'nnrTG
Cuts at: 426 980 1500 1504 1736 2749 2876 426
Size: 554 520 4 232 1013 127 2310
Fragments arranged by size:
2310 1013 554 520 232 127 4
MspA1I CmG'CkG
Cuts at: 1871 2185 2545 3329 3574 1871
Size: 314 360 784 245 3057
Fragments arranged by size:
3057 784 360 314 245
NaeI GCC'GGC
Cuts at: 2941 2941
Size: 4760
NarI GG'CG_CC
Cuts at: 2191 2439 2191
Size: 248 4512
NdeI CA'TA_TG
Cuts at: 965 965
Size: 4760
NgoMIV G'CCGG_C
Cuts at: 2939 2939
Size: 4760
NspI r_CATG'y
Cuts at: 397 1219 1473 1557 1735 1813 2313 3916 397
Size: 822 254 84 178 78 500 1603 1241
Fragments arranged by size:
1603 1241 822 500 254 178 84 78
PciI A'CATG_T
Cuts at: 393 3912 393
Size: 3519 1241
PmeI GTTT'AAAC
Cuts at: 1759 1759
Size: 4760
PmlI CAC'GTG
Cuts at: 1502 1538 1502
Size: 36 4724
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3206 3238 3206
Size: 32 4728
PsiI TTA'TAA
Cuts at: 911 911
Size: 4760
PstI C_TGCA'G
Cuts at: 1807 2492 1807
Size: 685 4075
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4760
PvuII CAG'CTG
Cuts at: 2185 2545 2185
Size: 360 4400
RsrII CG'GwC_CG
Cuts at: 2955 2955
Size: 4760
SacI G_AGCT'C
Cuts at: 1774 1774
Size: 4760
SalI G'TCGA_C
Cuts at: 1797 1797
Size: 4760
SapI GCTCTTCn'nnn_
Cuts at: 157 2783 2993 157
Size: 2626 210 1924
Fragments arranged by size:
2626 1924 210
SbfI CC_TGCA'GG
Cuts at: 1807 1807
Size: 4760
ScaI AGT'ACT
Cuts at: 4277 4277
Size: 4760
SexAI A'CCwGG_T
Cuts at: 856 856
Size: 4760
SfcI C'TryA_G
Cuts at: 33 1803 2488 3456 3647 33
Size: 1770 685 968 191 1146
Fragments arranged by size:
1770 1146 968 685 191
SfoI GGC'GCC
Cuts at: 2192 2440 2192
Size: 248 4512
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4760
SmaI CCC'GGG
Cuts at: 1782 1782
Size: 4760
SmlI C'TyrA_G
Cuts at: 385 954 1915 2830 3544 3806 385
Size: 569 961 915 714 262 1339
Fragments arranged by size:
1339 961 915 714 569 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4760
SphI G_CATG'C
Cuts at: 1219 1735 1813 2313 1219
Size: 516 78 500 3666
Fragments arranged by size:
3666 516 500 78
SspI AAT'ATT
Cuts at: 792 792
Size: 4760
StuI AGG'CCT
Cuts at: 1270 1270
Size: 4760
StyI C'CwwG_G
Cuts at: 1894 2248 1894
Size: 354 4406
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 970 2343 2476 2935 3144 970
Size: 1373 133 459 209 2586
Fragments arranged by size:
2586 1373 459 209 133
TatI w'GTAC_w
Cuts at: 4275 4275
Size: 4760
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 657 689 4277 4309 657
Size: 32 3588 32 1108
Fragments arranged by size:
3588 1108 32 32
Tth111I GACn'n_nGTC
Cuts at: 2557 2557
Size: 4760
XbaI T'CTAG_A
Cuts at: 1791 1791
Size: 4760
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2255 2255
Size: 4760
XmaI C'CCGG_G
Cuts at: 1780 1780
Size: 4760
XmnI GAAnn'nnTTC
Cuts at: 598 850 1186 1606 1690 598
Size: 252 336 420 84 3668
Fragments arranged by size:
3668 420 336 252 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PciI PmeI PmlI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI Bpu10I BseRI BsiWI BsmBI BspEI BsrGI
BssHII BstEII BstXI Bsu36I BtgI ClaI DraIII FseI
FspAI MfeI NcoI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI