Restriction map for
pF1KB9773
(Circular) (Six-base) MAPSORT of: pF1KB9773.seq Check: 3829 from: 1 to: 5036
pF1KB9773 5036 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 408 1899 408
Size: 1491 3545
AccI GT'mk_AC
Cuts at: 2074 2074
Size: 5036
Acc65I G'GTAC_C
Cuts at: 2052 2052
Size: 5036
AclI AA'CG_TT
Cuts at: 4876 4876
Size: 5036
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2861 3293 3646 4308 2861
Size: 432 353 662 3589
Fragments arranged by size:
3589 662 432 353
AflIII A'CryG_T
Cuts at: 725 989 2388 4188 4469 725
Size: 264 1399 1800 281 1292
Fragments arranged by size:
1800 1399 1292 281 264
AleI CACnn'nnGTG
Cuts at: 791 791
Size: 5036
AlwNI CAG_nnn'CTG
Cuts at: 1152 1278 2341 3779 1152
Size: 126 1063 1438 2409
Fragments arranged by size:
2409 1438 1063 126
ApaLI G'TGCA_C
Cuts at: 3874 3874
Size: 5036
ApoI r'AATT_y
Cuts at: 2040 4389 4645 2040
Size: 2349 256 2431
Fragments arranged by size:
2431 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5036
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5036
AvaI C'yCGr_G
Cuts at: 454 1414 1957 2056 454
Size: 960 543 99 3434
Fragments arranged by size:
3434 960 543 99
BamHI G'GATC_C
Cuts at: 2061 2061
Size: 5036
BanI G'GyrC_C
Cuts at: 142 715 1945 2052 2466 2714 2749 142
Size: 573 1230 107 414 248 35 2429
Fragments arranged by size:
2429 1230 573 414 248 107 35
BanII G_rGCy'C
Cuts at: 608 635 1055 1126 1781 1980 2050 3080 608
Size: 27 420 71 655 199 70 1030 2564
Fragments arranged by size:
2564 1030 655 420 199 71 70 27
BbeI G_GCGC'C
Cuts at: 2470 2718 2470
Size: 248 4788
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1531 1565 1531
Size: 34 5002
BciVI GTATCCnnnnn_n'
Cuts at: 2934 3990 5036 2934
Size: 1056 1046 2934
Fragments arranged by size:
2934 1056 1046
BclI T'GATC_A
Cuts at: 389 536 1346 2556 389
Size: 147 810 1210 2869
Fragments arranged by size:
2869 1210 810 147
BglI GCCn_nnn'nGGC
Cuts at: 1222 1222
Size: 5036
BglII A'GATC_T
Cuts at: 461 1385 2551 4588 461
Size: 924 1166 2037 909
Fragments arranged by size:
2037 1166 924 909
BlpI GC'TnA_GC
Cuts at: 1489 2148 1489
Size: 659 4377
Bme1580I G_kGCm'C
Cuts at: 145 718 1523 2661 2754 3878 145
Size: 573 805 1138 93 1124 1303
Fragments arranged by size:
1303 1138 1124 805 573 93
BmgBI CAC'GTC
Cuts at: 726 992 1293 726
Size: 266 301 4469
Fragments arranged by size:
4469 301 266
BmrI ACTGGGnnnn_n'
Cuts at: 2248 2329 2664 4808 2248
Size: 81 335 2144 2476
Fragments arranged by size:
2476 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 480 978 1041 1857 3403 480
Size: 498 63 816 1546 2113
Fragments arranged by size:
2113 1546 816 498 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 973 1069 1336 1753 1924 973
Size: 96 267 417 171 4085
Fragments arranged by size:
4085 417 267 171 96
Bpu10I CC'TnA_GC
Cuts at: 1636 1979 1636
Size: 343 4693
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1765 2212 3091 3805 4103 1765
Size: 447 879 714 298 2698
Fragments arranged by size:
2698 879 714 447 298
BsaI GGTCTCn'nnnn_
Cuts at: 4740 4740
Size: 5036
BsaAI yAC'GTr
Cuts at: 231 2389 231
Size: 2158 2878
BsaBI GATnn'nnATC
Cuts at: 6 130 1612 2550 4587 6
Size: 124 1482 938 2037 455
Fragments arranged by size:
2037 1482 938 455 124
BsaHI Gr'CG_yC
Cuts at: 1076 1853 2467 2715 3417 4913 1076
Size: 777 614 248 702 1496 1199
Fragments arranged by size:
1496 1199 777 702 614 248
BsaWI w'CCGG_w
Cuts at: 2225 2746 3424 3835 3982 4256 2225
Size: 521 678 411 147 274 3005
Fragments arranged by size:
3005 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 451 481 1897 1927 451
Size: 30 1416 30 3560
Fragments arranged by size:
3560 1416 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 472 850 1030 1660 2035 472
Size: 378 180 630 375 3473
Fragments arranged by size:
3473 630 378 375 180
BseYI C'CCAG_C
Cuts at: 223 1308 2461 3219 3884 4292 223
Size: 1085 1153 758 665 408 967
Fragments arranged by size:
1153 1085 967 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 436 1165 1252 1291 1684 1720 376
Size: 60 729 87 39 393 36 3692
Fragments arranged by size:
3692 729 393 87 60 39 36
BsiEI CG_ry'CG
Cuts at: 104 128 333 641 743 776 1325 2578 2624
Size: 24 205 308 102 33 549 1253 46
Cuts at: 2624 3854 104
Size: 1230 1286
Fragments arranged by size:
1286 1253 1230 549 308 205 102 46
33 24
BsiHKAI G_wGCw'C
Cuts at: 695 1027 1591 1889 2050 2828 3018 3878 695
Size: 332 564 298 161 778 190 860 1853
Fragments arranged by size:
1853 860 778 564 332 298 190 161
BsmI GAATG_Cn'
Cuts at: 445 445
Size: 5036
BsmBI CGTCTCn'nnnn_
Cuts at: 1312 1312
Size: 5036
Bsp1286I G_dGCh'C
Cuts at: 145 608 635 695 718 1027 1055 1126 1523
Size: 463 27 60 23 309 28 71 397
Cuts at: 1523 1591 1781 1889 1980 2050 2661 2754 2828
Size: 68 190 108 91 70 611 93 74
Cuts at: 2828 3018 3080 3878 145
Size: 190 62 798 1303
Fragments arranged by size:
1303 798 611 463 397 309 190 190
108 93 91 74 71 70 68 62
60 28 27 23
BspHI T'CATG_A
Cuts at: 3468 3468
Size: 5036
BspMI ACCTGCnnnn'nnnn_
Cuts at: 408 1286 1899 2086 2300 2602 2983 408
Size: 878 613 187 214 302 381 2461
Fragments arranged by size:
2461 878 613 381 302 214 187
BsrBI CCG'CTC
Cuts at: 50 252 1493 3328 3382 4866 5031 50
Size: 202 1241 1835 54 1484 165 55
Fragments arranged by size:
1835 1484 1241 202 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2948 2948
Size: 5036
BsrFI r'CCGG_y
Cuts at: 546 1591 1948 3034 3215 546
Size: 1045 357 1086 181 2367
Fragments arranged by size:
2367 1086 1045 357 181
BssSI C'ACGA_G
Cuts at: 1842 3307 4015 1842
Size: 1465 708 2863
Fragments arranged by size:
2863 1465 708
BstAPI GCAn_nnn'nTGC
Cuts at: 905 1152 1278 2413 905
Size: 247 126 1135 3528
Fragments arranged by size:
3528 1135 247 126
BstBI TT'CG_AA
Cuts at: 3397 3397
Size: 5036
BstEII G'GTnAC_C
Cuts at: 522 1533 522
Size: 1011 4025
BstXI CCAn_nnnn'nTGG
Cuts at: 231 793 1248 1863 231
Size: 562 455 615 3404
Fragments arranged by size:
3404 615 562 455
BstYI r'GATC_y
Cuts at: 1 461 1385 2061 2528 2551 2886 3132 3438
Size: 460 924 676 467 23 335 246 306
Cuts at: 3438 3450 3536 4588 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 924 676 467 460 449 335 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 119 470 635 770 1055 1577 1763 107
Size: 12 351 165 135 285 522 186 3380
Fragments arranged by size:
3380 522 351 285 186 165 135 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 177 633 2986 3167 3330 177
Size: 456 2353 181 163 1883
Fragments arranged by size:
2353 1883 456 181 163
BtsI GCAGTG_nn'
Cuts at: 2279 2279
Size: 5036
ClaI AT'CG_AT
Cuts at: 1547 1547
Size: 5036
DraI TTT'AAA
Cuts at: 2035 2035
Size: 5036
DraIII CAC_nnn'GTG
Cuts at: 831 831
Size: 5036
DrdI GACnn_nn'nnGTC
Cuts at: 930 2742 4086 930
Size: 1812 1344 1880
Fragments arranged by size:
1880 1812 1344
EaeI y'GGCC_r
Cuts at: 110 269 330 638 773 1238 1322 1574 1865
Size: 159 61 308 135 465 84 252 291
Cuts at: 1865 2621 2795 3186 3213 4558 110
Size: 756 174 391 27 1345 588
Fragments arranged by size:
1345 756 588 465 391 308 291 252
174 159 135 84 61 27
EagI C'GGCC_G
Cuts at: 330 638 773 1322 2621 330
Size: 308 135 549 1299 2745
Fragments arranged by size:
2745 1299 549 308 135
EarI CTCTTCn'nnn_
Cuts at: 1750 3059 3269 1750
Size: 1309 210 3517
Fragments arranged by size:
3517 1309 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1687 3244 3986 4132 4846 1687
Size: 1557 742 146 714 1877
Fragments arranged by size:
1877 1557 742 714 146
EcoICRI GAG'CTC
Cuts at: 2048 2048
Size: 5036
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 973 1069 1336 1753 1924 2861 3293 3646 4308
Size: 96 267 417 171 937 432 353 662
Cuts at: 4308 973
Size: 1701
Fragments arranged by size:
1701 937 662 432 417 353 267 171
96
EcoNI CCTnn'n_nnAGG
Cuts at: 909 970 1639 909
Size: 61 669 4306
Fragments arranged by size:
4306 669 61
EcoO109I rG'GnC_Cy
Cuts at: 923 1275 1984 2008 2175 923
Size: 352 709 24 167 3784
Fragments arranged by size:
3784 709 352 167 24
EcoRI G'AATT_C
Cuts at: 2040 2040
Size: 5036
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3055 3055
Size: 5036
FspI TGC'GCA
Cuts at: 898 2817 898
Size: 1919 3117
HaeII r_GCGC'y
Cuts at: 289 573 1482 1763 2470 2718 3948 4719 289
Size: 284 909 281 707 248 1230 771 606
Fragments arranged by size:
1230 909 771 707 606 284 281 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 451 483 1699 1731 2878 2908 2910 2940 451
Size: 32 1216 32 1147 30 2 30 2547
Fragments arranged by size:
2547 1216 1147 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2075 2075
Size: 5036
KasI G'GCGC_C
Cuts at: 2466 2714 2466
Size: 248 4788
KpnI G_GTAC'C
Cuts at: 2056 2056
Size: 5036
MluI A'CGCG_T
Cuts at: 4469 4469
Size: 5036
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 195 341 1052 1427 3795 3979 195
Size: 146 711 375 2368 184 1252
Fragments arranged by size:
2368 1252 711 375 184 146
MscI TGG'CCA
Cuts at: 112 2797 112
Size: 2685 2351
MslI CAynn'nnrTG
Cuts at: 791 1246 3025 3152 791
Size: 455 1779 127 2675
Fragments arranged by size:
2675 1779 455 127
MspA1I CmG'CkG
Cuts at: 121 1931 2147 2461 2821 3605 3850 121
Size: 1810 216 314 360 784 245 1307
Fragments arranged by size:
1810 1307 784 360 314 245 216
NaeI GCC'GGC
Cuts at: 548 3217 548
Size: 2669 2367
NarI GG'CG_CC
Cuts at: 2467 2715 2467
Size: 248 4788
NcoI C'CATG_G
Cuts at: 107 470 107
Size: 363 4673
NgoMIV G'CCGG_C
Cuts at: 546 3215 546
Size: 2669 2367
NspI r_CATG'y
Cuts at: 2089 2589 4192 2089
Size: 500 1603 2933
Fragments arranged by size:
2933 1603 500
PasI CC'CwG_GG
Cuts at: 1511 1511
Size: 5036
PciI A'CATG_T
Cuts at: 4188 4188
Size: 5036
PflMI CCAn_nnn'nTGG
Cuts at: 831 831
Size: 5036
PmeI GTTT'AAAC
Cuts at: 2035 2035
Size: 5036
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3482 3514 3482
Size: 32 5004
PpuMI rG'GwC_Cy
Cuts at: 923 1275 1984 923
Size: 352 709 3975
Fragments arranged by size:
3975 709 352
PshAI GACnn'nnGTC
Cuts at: 1826 1826
Size: 5036
PstI C_TGCA'G
Cuts at: 1160 2083 2768 1160
Size: 923 685 3428
Fragments arranged by size:
3428 923 685
PvuI CG_AT'CG
Cuts at: 104 128 743 104
Size: 24 615 4397
Fragments arranged by size:
4397 615 24
PvuII CAG'CTG
Cuts at: 2461 2821 2461
Size: 360 4676
RsrII CG'GwC_CG
Cuts at: 3231 3231
Size: 5036
SacI G_AGCT'C
Cuts at: 2050 2050
Size: 5036
SacII CC_GC'GG
Cuts at: 122 122
Size: 5036
SalI G'TCGA_C
Cuts at: 2073 2073
Size: 5036
SanDI GG'GwC_CC
Cuts at: 1984 1984
Size: 5036
SapI GCTCTTCn'nnn_
Cuts at: 1750 3059 3269 1750
Size: 1309 210 3517
Fragments arranged by size:
3517 1309 210
SbfI CC_TGCA'GG
Cuts at: 2083 2083
Size: 5036
ScaI AGT'ACT
Cuts at: 4553 4553
Size: 5036
SexAI A'CCwGG_T
Cuts at: 278 1106 278
Size: 828 4208
SfcI C'TryA_G
Cuts at: 33 1156 2079 2764 3732 3923 33
Size: 1123 923 685 968 191 1146
Fragments arranged by size:
1146 1123 968 923 685 191
SfoI GGC'GCC
Cuts at: 2468 2716 2468
Size: 248 4788
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5036
SmaI CCC'GGG
Cuts at: 1416 1959 2058 1416
Size: 543 99 4394
Fragments arranged by size:
4394 543 99
SmlI C'TyrA_G
Cuts at: 1780 2191 3106 3820 4082 1780
Size: 411 915 714 262 2734
Fragments arranged by size:
2734 915 714 411 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5036
SphI G_CATG'C
Cuts at: 2089 2589 2089
Size: 500 4536
StyI C'CwwG_G
Cuts at: 107 410 440 470 581 1139 1862 1988 2170
Size: 303 30 30 111 558 723 126 182
Cuts at: 2170 2524 107
Size: 354 2619
Fragments arranged by size:
2619 723 558 354 303 182 126 111
30 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 781 799 955 2619 2752 3211 3420 781
Size: 18 156 1664 133 459 209 2397
Fragments arranged by size:
2397 1664 459 209 156 133 18
TatI w'GTAC_w
Cuts at: 1679 4551 1679
Size: 2872 2164
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1897 1929 4553 4585 1897
Size: 32 2624 32 2348
Fragments arranged by size:
2624 2348 32 32
Tth111I GACn'n_nGTC
Cuts at: 927 1108 2833 927
Size: 181 1725 3130
Fragments arranged by size:
3130 1725 181
XbaI T'CTAG_A
Cuts at: 2067 2067
Size: 5036
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 279 1728 1948 2531 279
Size: 1449 220 583 2784
Fragments arranged by size:
2784 1449 583 220
XmaI C'CCGG_G
Cuts at: 1414 1957 2056 1414
Size: 543 99 4394
Fragments arranged by size:
4394 543 99
XmnI GAAnn'nnTTC
Cuts at: 826 826
Size: 5036
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmtI BsiWI
BspEI BsrGI BssHII BstZ17I Bsu36I CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PsiI PspOMI PspXI PsrI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI