Restriction map for
pF1KB9774
(Circular) (Six-base) MAPSORT of: pF1KB9774.seq Check: 4036 from: 1 to: 5126
pF1KB9774 5126 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1880 2060 1880
Size: 180 4946
AccI GT'mk_AC
Cuts at: 710 2164 710
Size: 1454 3672
Acc65I G'GTAC_C
Cuts at: 2142 2142
Size: 5126
AclI AA'CG_TT
Cuts at: 4966 4966
Size: 5126
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2951 3383 4130 4398 2951
Size: 432 747 268 3679
Fragments arranged by size:
3679 747 432 268
AflIII A'CryG_T
Cuts at: 1997 2478 3582 4559 1997
Size: 481 1104 977 2564
Fragments arranged by size:
2564 1104 977 481
AgeI A'CCGG_T
Cuts at: 3514 3514
Size: 5126
AhdI GACnn_n'nnGTC
Cuts at: 1086 1200 1086
Size: 114 5012
AlwNI CAG_nnn'CTG
Cuts at: 144 159 198 465 1395 1470 1604 1890 1956
Size: 15 39 267 930 75 134 286 66
Cuts at: 1956 2431 3998 144
Size: 475 1567 1272
Fragments arranged by size:
1567 1272 930 475 286 267 134 75
66 39 15
ApaI G_GGCC'C
Cuts at: 192 2080 192
Size: 1888 3238
ApaLI G'TGCA_C
Cuts at: 1919 2059 3896 1919
Size: 140 1837 3149
Fragments arranged by size:
3149 1837 140
ApoI r'AATT_y
Cuts at: 532 2130 4479 4735 532
Size: 1598 2349 256 923
Fragments arranged by size:
2349 1598 923 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5126
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5126
AvaI C'yCGr_G
Cuts at: 666 1422 1434 1474 1669 1782 2073 2146 666
Size: 756 12 40 195 113 291 73 3646
Fragments arranged by size:
3646 756 291 195 113 73 40 12
BamHI G'GATC_C
Cuts at: 927 1161 2151 927
Size: 234 990 3902
Fragments arranged by size:
3902 990 234
BanI G'GyrC_C
Cuts at: 339 954 1497 1642 2083 2142 2556 2804 2839
Size: 615 543 145 441 59 414 248 35
Cuts at: 2839 339
Size: 2626
Fragments arranged by size:
2626 615 543 441 414 248 145 59
35
BanII G_rGCy'C
Cuts at: 192 374 832 869 950 2080 2140 3170 192
Size: 182 458 37 81 1130 60 1030 2148
Fragments arranged by size:
2148 1130 1030 458 182 81 60 37
BbeI G_GCGC'C
Cuts at: 1501 1646 2087 2560 2808 1501
Size: 145 441 473 248 3819
Fragments arranged by size:
3819 473 441 248 145
BbsI GAAGACnn'nnnn_
Cuts at: 339 740 1855 339
Size: 401 1115 3610
Fragments arranged by size:
3610 1115 401
BbvCI CC'TCA_GC
Cuts at: 137 137
Size: 5126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1668 1702 90
Size: 34 1544 34 3514
Fragments arranged by size:
3514 1544 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3024 3785 5126 3024
Size: 761 1341 3024
Fragments arranged by size:
3024 1341 761
BclI T'GATC_A
Cuts at: 2646 2646
Size: 5126
BglI GCCn_nnn'nGGC
Cuts at: 424 1784 424
Size: 1360 3766
BglII A'GATC_T
Cuts at: 392 2641 4678 392
Size: 2249 2037 840
Fragments arranged by size:
2249 2037 840
BlpI GC'TnA_GC
Cuts at: 863 2238 863
Size: 1375 3751
Bme1580I G_kGCm'C
Cuts at: 192 342 1669 1923 2063 2080 2751 2844 3900
Size: 150 1327 254 140 17 671 93 1056
Cuts at: 3900 192
Size: 1418
Fragments arranged by size:
1418 1327 1056 671 254 150 140 93
17
BmrI ACTGGGnnnn_n'
Cuts at: 369 2338 2419 2754 4898 369
Size: 1969 81 335 2144 597
Fragments arranged by size:
2144 1969 597 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1503 3493 1503
Size: 1990 3136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 201 493 631 836 1045 201
Size: 292 138 205 209 4282
Fragments arranged by size:
4282 292 209 205 138
Bpu10I CC'TnA_GC
Cuts at: 137 2111 137
Size: 1974 3152
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 1945 2026 2302 3181 3673 3971 260
Size: 1685 81 276 879 492 298 1415
Fragments arranged by size:
1685 1415 879 492 298 276 81
BsaI GGTCTCn'nnnn_
Cuts at: 4830 4830
Size: 5126
BsaAI yAC'GTr
Cuts at: 2000 2479 2000
Size: 479 4647
BsaBI GATnn'nnATC
Cuts at: 6 2640 4677 6
Size: 2634 2037 455
Fragments arranged by size:
2634 2037 455
BsaHI Gr'CG_yC
Cuts at: 293 1498 1643 2084 2557 2805 3507 5003 293
Size: 1205 145 441 473 248 702 1496 416
Fragments arranged by size:
1496 1205 702 473 441 416 248 145
BsaWI w'CCGG_w
Cuts at: 2315 2836 3514 3788 3935 4346 2315
Size: 521 678 274 147 411 3095
Fragments arranged by size:
3095 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 541 571 1099 1129 2081 2111 541
Size: 30 528 30 952 30 3556
Fragments arranged by size:
3556 952 528 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 223 1120 1234 1621 223
Size: 897 114 387 3728
Fragments arranged by size:
3728 897 387 114
BseYI C'CCAG_C
Cuts at: 144 185 567 1337 1343 1349 1382 2551 3309
Size: 41 382 770 6 6 33 1169 758
Cuts at: 3309 3886 4382 144
Size: 577 496 888
Fragments arranged by size:
1169 888 770 758 577 496 382 41
33 6 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1621 1876 1621
Size: 255 4871
BsiEI CG_ry'CG
Cuts at: 104 130 357 527 2668 2714 3922 104
Size: 26 227 170 2141 46 1208 1308
Fragments arranged by size:
2141 1308 1208 227 170 46 26
BsiHKAI G_wGCw'C
Cuts at: 1837 1871 1923 2063 2140 2918 3108 3900 1837
Size: 34 52 140 77 778 190 792 3063
Fragments arranged by size:
3063 792 778 190 140 77 52 34
BsmI GAATG_Cn'
Cuts at: 263 263
Size: 5126
Bsp1286I G_dGCh'C
Cuts at: 192 342 374 832 869 950 1669 1837 1871
Size: 150 32 458 37 81 719 168 34
Cuts at: 1871 1923 2063 2080 2140 2751 2844 2918 3108
Size: 52 140 17 60 611 93 74 190
Cuts at: 3108 3170 3900 192
Size: 62 730 1418
Fragments arranged by size:
1418 730 719 611 458 190 168 150
140 93 81 74 62 60 52 37
34 32 17
BspEI T'CCGG_A
Cuts at: 4346 4346
Size: 5126
BspHI T'CATG_A
Cuts at: 4302 4302
Size: 5126
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1880 1964 2060 2176 2390 2692 3073 1880
Size: 84 96 116 214 302 381 3933
Fragments arranged by size:
3933 381 302 214 116 96 84
BsrBI CCG'CTC
Cuts at: 50 1737 1821 1905 3418 3472 4956 5121 50
Size: 1687 84 84 1513 54 1484 165 55
Fragments arranged by size:
1687 1513 1484 165 84 84 55 54
BsrDI GCAATG_nn'
Cuts at: 713 3038 713
Size: 2325 2801
BsrFI r'CCGG_y
Cuts at: 228 3124 3305 3514 228
Size: 2896 181 209 1840
Fragments arranged by size:
2896 1840 209 181
BssSI C'ACGA_G
Cuts at: 1662 3397 3755 1662
Size: 1735 358 3033
Fragments arranged by size:
3033 1735 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1604 1956 2503 1604
Size: 352 547 4227
Fragments arranged by size:
4227 547 352
BstBI TT'CG_AA
Cuts at: 3487 3487
Size: 5126
BstXI CCAn_nnnn'nTGG
Cuts at: 1235 1410 1235
Size: 175 4951
BstYI r'GATC_y
Cuts at: 1 392 927 1161 2151 2618 2641 2976 3222
Size: 391 535 234 990 467 23 335 246
Cuts at: 3222 4234 4320 4332 4678 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 990 535 467 449 391 346 335
246 234 86 23 12
BtgI C'CryG_G
Cuts at: 527 1157 1234 1812 2089 527
Size: 630 77 578 277 3564
Fragments arranged by size:
3564 630 578 277 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1389 1553 3076 3257 3420 1389
Size: 164 1523 181 163 3095
Fragments arranged by size:
3095 1523 181 164 163
BtsI GCAGTG_nn'
Cuts at: 2369 2369
Size: 5126
DraI TTT'AAA
Cuts at: 2125 2125
Size: 5126
DraIII CAC_nnn'GTG
Cuts at: 1001 1001
Size: 5126
DrdI GACnn_nn'nnGTC
Cuts at: 2832 3690 2832
Size: 858 4268
EaeI y'GGCC_r
Cuts at: 127 248 301 506 524 1412 2711 2885 3276
Size: 121 53 205 18 888 1299 174 391
Cuts at: 3276 3303 4648 127
Size: 27 1345 605
Fragments arranged by size:
1345 1299 888 605 391 205 174 121
53 27 18
EagI C'GGCC_G
Cuts at: 127 524 2711 127
Size: 397 2187 2542
Fragments arranged by size:
2542 2187 397
EarI CTCTTCn'nnn_
Cuts at: 902 1759 3149 3359 902
Size: 857 1390 210 2669
Fragments arranged by size:
2669 1390 857 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1638 1783 3334 3644 3790 4936 1638
Size: 145 1551 310 146 1146 1828
Fragments arranged by size:
1828 1551 1146 310 146 145
EcoICRI GAG'CTC
Cuts at: 2138 2138
Size: 5126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 201 493 631 836 1045 2951 3383 4130 4398
Size: 292 138 205 209 1906 432 747 268
Cuts at: 4398 201
Size: 929
Fragments arranged by size:
1906 929 747 432 292 268 209 205
138
EcoNI CCTnn'n_nnAGG
Cuts at: 580 787 580
Size: 207 4919
EcoO109I rG'GnC_Cy
Cuts at: 156 572 651 684 1020 1080 2077 2265 156
Size: 416 79 33 336 60 997 188 3017
Fragments arranged by size:
3017 997 416 336 188 79 60 33
EcoRI G'AATT_C
Cuts at: 2130 2130
Size: 5126
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3145 3145
Size: 5126
FspI TGC'GCA
Cuts at: 634 962 1696 2907 634
Size: 328 734 1211 2853
Fragments arranged by size:
2853 1211 734 328
HaeII r_GCGC'y
Cuts at: 235 900 1501 1646 1992 2087 2560 2808 3830
Size: 665 601 145 346 95 473 248 1022
Cuts at: 3830 4809 235
Size: 979 552
Fragments arranged by size:
1022 979 665 601 552 473 346 248
145 95
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 958 990 1072 1104 1186 1218 2968 2998 3000
Size: 32 82 32 82 32 1750 30 2
Cuts at: 3000 3030 958
Size: 30 3054
Fragments arranged by size:
3054 1750 82 82 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 122 711 2165 122
Size: 589 1454 3083
Fragments arranged by size:
3083 1454 589
KasI G'GCGC_C
Cuts at: 1497 1642 2083 2556 2804 1497
Size: 145 441 473 248 3819
Fragments arranged by size:
3819 473 441 248 145
KpnI G_GTAC'C
Cuts at: 2146 2146
Size: 5126
MluI A'CGCG_T
Cuts at: 4559 4559
Size: 5126
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3797 3981 3797
Size: 184 4942
MscI TGG'CCA
Cuts at: 2887 2887
Size: 5126
MslI CAynn'nnrTG
Cuts at: 718 1233 1408 3115 3242 718
Size: 515 175 1707 127 2602
Fragments arranged by size:
2602 1707 515 175 127
MspA1I CmG'CkG
Cuts at: 144 173 462 1017 1368 1601 1814 1887 2066
Size: 29 289 555 351 233 213 73 179
Cuts at: 2066 2237 2551 2911 3924 4169 144
Size: 171 314 360 1013 245 1101
Fragments arranged by size:
1101 1013 555 360 351 314 289 245
233 213 179 171 73 29
NaeI GCC'GGC
Cuts at: 230 3307 230
Size: 3077 2049
NarI GG'CG_CC
Cuts at: 1498 1643 2084 2557 2805 1498
Size: 145 441 473 248 3819
Fragments arranged by size:
3819 473 441 248 145
NcoI C'CATG_G
Cuts at: 1234 1234
Size: 5126
NgoMIV G'CCGG_C
Cuts at: 228 3305 228
Size: 3077 2049
NspI r_CATG'y
Cuts at: 2179 2679 3586 2179
Size: 500 907 3719
Fragments arranged by size:
3719 907 500
PasI CC'CwG_GG
Cuts at: 869 869
Size: 5126
PciI A'CATG_T
Cuts at: 3582 3582
Size: 5126
PflMI CCAn_nnn'nTGG
Cuts at: 159 159
Size: 5126
PfoI T'CCnGG_A
Cuts at: 1090 1090
Size: 5126
PmeI GTTT'AAAC
Cuts at: 2125 2125
Size: 5126
PmlI CAC'GTG
Cuts at: 2000 2000
Size: 5126
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4265 4297 4265
Size: 32 5094
PpuMI rG'GwC_Cy
Cuts at: 572 651 1080 572
Size: 79 429 4618
Fragments arranged by size:
4618 429 79
PshAI GACnn'nnGTC
Cuts at: 706 706
Size: 5126
PsiI TTA'TAA
Cuts at: 776 776
Size: 5126
PspOMI G'GGCC_C
Cuts at: 188 2076 188
Size: 1888 3238
PstI C_TGCA'G
Cuts at: 584 602 1373 1877 2173 2858 584
Size: 18 771 504 296 685 2852
Fragments arranged by size:
2852 771 685 504 296 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5126
PvuII CAG'CTG
Cuts at: 144 462 1368 1601 2551 2911 144
Size: 318 906 233 950 360 2359
Fragments arranged by size:
2359 950 906 360 318 233
RsrII CG'GwC_CG
Cuts at: 3321 3321
Size: 5126
SacI G_AGCT'C
Cuts at: 2140 2140
Size: 5126
SacII CC_GC'GG
Cuts at: 1815 1815
Size: 5126
SalI G'TCGA_C
Cuts at: 709 2163 709
Size: 1454 3672
SanDI GG'GwC_CC
Cuts at: 572 572
Size: 5126
SapI GCTCTTCn'nnn_
Cuts at: 1759 3149 3359 1759
Size: 1390 210 3526
Fragments arranged by size:
3526 1390 210
SbfI CC_TGCA'GG
Cuts at: 2173 2173
Size: 5126
ScaI AGT'ACT
Cuts at: 4643 4643
Size: 5126
SfcI C'TryA_G
Cuts at: 33 580 598 1369 1854 1873 2169 2854 3847
Size: 547 18 771 485 19 296 685 993
Cuts at: 3847 4038 33
Size: 191 1121
Fragments arranged by size:
1121 993 771 685 547 485 296 191
19 18
SfoI GGC'GCC
Cuts at: 1499 1644 2085 2558 2806 1499
Size: 145 441 473 248 3819
Fragments arranged by size:
3819 473 441 248 145
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5126
SmaI CCC'GGG
Cuts at: 668 1424 1476 2075 2148 668
Size: 756 52 599 73 3646
Fragments arranged by size:
3646 756 599 73 52
SmlI C'TyrA_G
Cuts at: 275 1960 2041 2281 3196 3688 3950 275
Size: 1685 81 240 915 492 262 1451
Fragments arranged by size:
1685 1451 915 492 262 240 81
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5126
SphI G_CATG'C
Cuts at: 2179 2679 2179
Size: 500 4626
SrfI GCCC'GGGC
Cuts at: 2075 2075
Size: 5126
StyI C'CwwG_G
Cuts at: 689 1234 1730 2260 2614 689
Size: 545 496 530 354 3201
Fragments arranged by size:
3201 545 530 496 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2709 2842 3301 3510 2709
Size: 133 459 209 4325
Fragments arranged by size:
4325 459 209 133
TatI w'GTAC_w
Cuts at: 722 4641 722
Size: 3919 1207
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 332 364 541 573 985 1017 1099 1131 2081
Size: 32 177 32 412 32 82 32 950
Cuts at: 2081 2113 4643 4675 332
Size: 32 2530 32 783
Fragments arranged by size:
2530 950 783 412 177 82 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2923 2923
Size: 5126
XbaI T'CTAG_A
Cuts at: 2157 2157
Size: 5126
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 426 2621 426
Size: 2195 2931
XmaI C'CCGG_G
Cuts at: 666 1422 1474 2073 2146 666
Size: 756 52 599 73 3646
Fragments arranged by size:
3646 756 599 73 52
XmnI GAAnn'nnTTC
Cuts at: 1768 1768
Size: 5126
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PspXI PsrI SexAI SfiI SgrAI SnaBI SspI
StuI SwaI XhoI ZraI