Restriction map for
pF1KB9776
(Circular) (Six-base) MAPSORT of: pF1KB9776.seq Check: 5573 from: 1 to: 5261
pF1KB9776 5261 bp
With 182 enzymes: SgfI *
September 18, 2009 17:01 ..
AccI GT'mk_AC
Cuts at: 2299 2299
Size: 5261
Acc65I G'GTAC_C
Cuts at: 2277 2277
Size: 5261
AclI AA'CG_TT
Cuts at: 5101 5101
Size: 5261
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 795 3086 3518 4265 4533 795
Size: 2291 432 747 268 1523
Fragments arranged by size:
2291 1523 747 432 268
AfeI AGC'GCT
Cuts at: 1978 1978
Size: 5261
AflIII A'CryG_T
Cuts at: 2613 3717 4694 2613
Size: 1104 977 3180
Fragments arranged by size:
3180 1104 977
AgeI A'CCGG_T
Cuts at: 3649 3649
Size: 5261
AhdI GACnn_n'nnGTC
Cuts at: 1896 1896
Size: 5261
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1960 1960
Size: 5261
AlwNI CAG_nnn'CTG
Cuts at: 2566 4133 2566
Size: 1567 3694
ApaI G_GGCC'C
Cuts at: 1091 2141 1091
Size: 1050 4211
ApaLI G'TGCA_C
Cuts at: 1075 4031 1075
Size: 2956 2305
ApoI r'AATT_y
Cuts at: 2265 4614 4870 2265
Size: 2349 256 2656
Fragments arranged by size:
2656 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5261
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5261
AvaI C'yCGr_G
Cuts at: 1686 1758 2058 2281 1686
Size: 72 300 223 4666
Fragments arranged by size:
4666 300 223 72
BamHI G'GATC_C
Cuts at: 2286 2286
Size: 5261
BanI G'GyrC_C
Cuts at: 1216 1648 1965 2277 2691 2939 2974 1216
Size: 432 317 312 414 248 35 3503
Fragments arranged by size:
3503 432 414 317 312 248 35
BanII G_rGCy'C
Cuts at: 358 1091 1163 1243 2141 2151 2275 3305 358
Size: 733 72 80 898 10 124 1030 2314
Fragments arranged by size:
2314 1030 898 733 124 80 72 10
BbeI G_GCGC'C
Cuts at: 1652 1969 2695 2943 1652
Size: 317 726 248 3970
Fragments arranged by size:
3970 726 317 248
BbsI GAAGACnn'nnnn_
Cuts at: 759 759
Size: 5261
BbvCI CC'TCA_GC
Cuts at: 350 350
Size: 5261
BciVI GTATCCnnnnn_n'
Cuts at: 3159 3920 5261 3159
Size: 761 1341 3159
Fragments arranged by size:
3159 1341 761
BclI T'GATC_A
Cuts at: 2781 2781
Size: 5261
BglI GCCn_nnn'nGGC
Cuts at: 1167 1167
Size: 5261
BglII A'GATC_T
Cuts at: 284 2776 4813 284
Size: 2492 2037 732
Fragments arranged by size:
2492 2037 732
BlpI GC'TnA_GC
Cuts at: 2373 2373
Size: 5261
Bme1580I G_kGCm'C
Cuts at: 1079 1091 2084 2141 2180 2886 2979 4035 1079
Size: 12 993 57 39 706 93 1056 2305
Fragments arranged by size:
2305 1056 993 706 93 57 39 12
BmrI ACTGGGnnnn_n'
Cuts at: 2225 2473 2554 2889 5033 2225
Size: 248 81 335 2144 2453
Fragments arranged by size:
2453 2144 335 248 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 303 531 711 819 3628 303
Size: 228 180 108 2809 1936
Fragments arranged by size:
2809 1936 228 180 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 1626 679
Size: 947 4314
Bpu10I CC'TnA_GC
Cuts at: 350 1249 1980 350
Size: 899 731 3631
Fragments arranged by size:
3631 899 731
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1393 2437 3316 3808 4106 1393
Size: 1044 879 492 298 2548
Fragments arranged by size:
2548 1044 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 109 655 1197 4965 109
Size: 546 542 3768 405
Fragments arranged by size:
3768 546 542 405
BsaAI yAC'GTr
Cuts at: 1132 2614 1132
Size: 1482 3779
BsaBI GATnn'nnATC
Cuts at: 6 1417 2775 4812 6
Size: 1411 1358 2037 455
Fragments arranged by size:
2037 1411 1358 455
BsaHI Gr'CG_yC
Cuts at: 151 1649 1966 2692 2940 3642 5138 151
Size: 1498 317 726 248 702 1496 274
Fragments arranged by size:
1498 1496 726 702 317 274 248
BsaWI w'CCGG_w
Cuts at: 1487 2450 2971 3649 3923 4070 4481 1487
Size: 963 521 678 274 147 411 2267
Fragments arranged by size:
2267 963 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 139 169 1624 1654 139
Size: 30 1455 30 3746
Fragments arranged by size:
3746 1455 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 295 1263 295
Size: 968 4293
BseYI C'CCAG_C
Cuts at: 226 369 530 1115 1773 1969 2686 3444 4021
Size: 143 161 585 658 196 717 758 577
Cuts at: 4021 4517 226
Size: 496 970
Fragments arranged by size:
970 758 717 658 585 577 496 196
161 143
BsiEI CG_ry'CG
Cuts at: 104 1860 2803 2849 4057 104
Size: 1756 943 46 1208 1308
Fragments arranged by size:
1756 1308 1208 943 46
BsiHKAI G_wGCw'C
Cuts at: 1079 1255 2003 2275 3053 3243 4035 1079
Size: 176 748 272 778 190 792 2305
Fragments arranged by size:
2305 792 778 748 272 190 176
BsmBI CGTCTCn'nnnn_
Cuts at: 142 502 142
Size: 360 4901
Bsp1286I G_dGCh'C
Cuts at: 358 1079 1091 1163 1243 1255 2003 2084 2141
Size: 721 12 72 80 12 748 81 57
Cuts at: 2141 2151 2180 2275 2886 2979 3053 3243 3305
Size: 10 29 95 611 93 74 190 62
Cuts at: 3305 4035 358
Size: 730 1584
Fragments arranged by size:
1584 748 730 721 611 190 95 93
81 80 74 72 62 57 29 12
12 10
BspEI T'CCGG_A
Cuts at: 4481 4481
Size: 5261
BspHI T'CATG_A
Cuts at: 950 4437 950
Size: 3487 1774
BspMI ACCTGCnnnn'nnnn_
Cuts at: 695 1526 2311 2525 2827 3208 695
Size: 831 785 214 302 381 2748
Fragments arranged by size:
2748 831 785 381 302 214
BsrBI CCG'CTC
Cuts at: 50 540 798 1962 3553 3607 5091 5256 50
Size: 490 258 1164 1591 54 1484 165 55
Fragments arranged by size:
1591 1484 1164 490 258 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1144 3173 1144
Size: 2029 3232
BsrFI r'CCGG_y
Cuts at: 1213 1986 3259 3440 3649 1213
Size: 773 1273 181 209 2825
Fragments arranged by size:
2825 1273 773 209 181
BssSI C'ACGA_G
Cuts at: 3532 3890 3532
Size: 358 4903
BstAPI GCAn_nnn'nTGC
Cuts at: 2638 2638
Size: 5261
BstBI TT'CG_AA
Cuts at: 3622 3622
Size: 5261
BstEII G'GTnAC_C
Cuts at: 1591 1591
Size: 5261
BstXI CCAn_nnnn'nTGG
Cuts at: 139 139
Size: 5261
BstYI r'GATC_y
Cuts at: 1 284 2286 2753 2776 3111 3357 4369 4455
Size: 283 2002 467 23 335 246 1012 86
Cuts at: 4455 4467 4813 1
Size: 12 346 449
Fragments arranged by size:
2002 1012 467 449 346 335 283 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 965 1361 1706 1954 2126 2132 2219 107
Size: 858 396 345 248 172 6 87 3149
Fragments arranged by size:
3149 858 396 345 248 172 87 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3211 3392 3555 3211
Size: 181 163 4917
Fragments arranged by size:
4917 181 163
BtsI GCAGTG_nn'
Cuts at: 657 738 1199 1579 1839 2504 657
Size: 81 461 380 260 665 3414
Fragments arranged by size:
3414 665 461 380 260 81
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 900 935 900
Size: 35 5226
DraI TTT'AAA
Cuts at: 2260 2260
Size: 5261
DraIII CAC_nnn'GTG
Cuts at: 1470 1470
Size: 5261
DrdI GACnn_nn'nnGTC
Cuts at: 1051 2967 3825 1051
Size: 1916 858 2487
Fragments arranged by size:
2487 1916 858
EaeI y'GGCC_r
Cuts at: 321 541 1610 1991 2027 2129 2216 2846 3020
Size: 220 1069 381 36 102 87 630 174
Cuts at: 3020 3411 3438 4783 321
Size: 391 27 1345 799
Fragments arranged by size:
1345 1069 799 630 391 381 220 174
102 87 36 27
EagI C'GGCC_G
Cuts at: 2846 2846
Size: 5261
EarI CTCTTCn'nnn_
Cuts at: 3284 3494 3284
Size: 210 5051
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 214 786 1528 3469 3779 3925 5071 214
Size: 572 742 1941 310 146 1146 404
Fragments arranged by size:
1941 1146 742 572 404 310 146
EcoICRI GAG'CTC
Cuts at: 2273 2273
Size: 5261
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 795 1626 3086 3518 4265 4533 679
Size: 116 831 1460 432 747 268 1407
Fragments arranged by size:
1460 1407 831 747 432 268 116
EcoNI CCTnn'n_nnAGG
Cuts at: 1376 1376
Size: 5261
EcoO109I rG'GnC_Cy
Cuts at: 1087 1119 1875 2137 2138 2400 1087
Size: 32 756 262 1 262 3948
Fragments arranged by size:
3948 756 262 262 32 1
EcoRI G'AATT_C
Cuts at: 2265 2265
Size: 5261
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 258 1578 3280 258
Size: 1320 1702 2239
Fragments arranged by size:
2239 1702 1320
FspI TGC'GCA
Cuts at: 3042 3042
Size: 5261
HaeII r_GCGC'y
Cuts at: 1308 1652 1802 1969 1980 2695 2943 3965 4944
Size: 344 150 167 11 715 248 1022 979
Cuts at: 4944 1308
Size: 1625
Fragments arranged by size:
1625 1022 979 715 344 248 167 150
11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 379 411 619 651 1882 1914 2052 2084 2155
Size: 32 208 32 1231 32 138 32 71
Cuts at: 2155 2187 3103 3133 3135 3165 379
Size: 32 916 30 2 30 2475
Fragments arranged by size:
2475 1231 916 208 138 71 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1551 2300 1551
Size: 749 4512
KasI G'GCGC_C
Cuts at: 1648 1965 2691 2939 1648
Size: 317 726 248 3970
Fragments arranged by size:
3970 726 317 248
KpnI G_GTAC'C
Cuts at: 2281 2281
Size: 5261
MluI A'CGCG_T
Cuts at: 4694 4694
Size: 5261
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1364 1807 1865 3932 4116 1364
Size: 443 58 2067 184 2509
Fragments arranged by size:
2509 2067 443 184 58
MscI TGG'CCA
Cuts at: 1612 2029 2218 3022 1612
Size: 417 189 804 3851
Fragments arranged by size:
3851 804 417 189
MslI CAynn'nnrTG
Cuts at: 137 464 470 643 2107 2218 3250 3377 137
Size: 327 6 173 1464 111 1032 127 2021
Fragments arranged by size:
2021 1464 1032 327 173 127 111 6
MspA1I CmG'CkG
Cuts at: 349 1910 1956 2128 2372 2686 3046 4059 4304
Size: 1561 46 172 244 314 360 1013 245
Cuts at: 4304 349
Size: 1306
Fragments arranged by size:
1561 1306 1013 360 314 245 244 172
46
NaeI GCC'GGC
Cuts at: 1988 3442 1988
Size: 1454 3807
NarI GG'CG_CC
Cuts at: 1649 1966 2692 2940 1649
Size: 317 726 248 3970
Fragments arranged by size:
3970 726 317 248
NcoI C'CATG_G
Cuts at: 107 1361 1706 2132 2219 107
Size: 1254 345 426 87 3149
Fragments arranged by size:
3149 1254 426 345 87
NgoMIV G'CCGG_C
Cuts at: 1986 3440 1986
Size: 1454 3807
NspI r_CATG'y
Cuts at: 571 1404 2314 2814 3721 571
Size: 833 910 500 907 2111
Fragments arranged by size:
2111 910 907 833 500
PciI A'CATG_T
Cuts at: 3717 3717
Size: 5261
PflMI CCAn_nnn'nTGG
Cuts at: 1115 1470 2025 1115
Size: 355 555 4351
Fragments arranged by size:
4351 555 355
PfoI T'CCnGG_A
Cuts at: 1766 2073 1766
Size: 307 4954
PmeI GTTT'AAAC
Cuts at: 2260 2260
Size: 5261
PmlI CAC'GTG
Cuts at: 1132 1132
Size: 5261
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4400 4432 4400
Size: 32 5229
PpuMI rG'GwC_Cy
Cuts at: 1119 1875 1119
Size: 756 4505
PshAI GACnn'nnGTC
Cuts at: 1124 1124
Size: 5261
PspOMI G'GGCC_C
Cuts at: 1087 2137 1087
Size: 1050 4211
PstI C_TGCA'G
Cuts at: 1206 2308 2993 1206
Size: 1102 685 3474
Fragments arranged by size:
3474 1102 685
PvuI CG_AT'CG
Cuts at: 104 1860 104
Size: 1756 3505
PvuII CAG'CTG
Cuts at: 349 2686 3046 349
Size: 2337 360 2564
Fragments arranged by size:
2564 2337 360
RsrII CG'GwC_CG
Cuts at: 1485 3456 1485
Size: 1971 3290
SacI G_AGCT'C
Cuts at: 2275 2275
Size: 5261
SacII CC_GC'GG
Cuts at: 1957 2129 1957
Size: 172 5089
SalI G'TCGA_C
Cuts at: 2298 2298
Size: 5261
SanDI GG'GwC_CC
Cuts at: 1119 1119
Size: 5261
SapI GCTCTTCn'nnn_
Cuts at: 3284 3494 3284
Size: 210 5051
SbfI CC_TGCA'GG
Cuts at: 2308 2308
Size: 5261
ScaI AGT'ACT
Cuts at: 4778 4778
Size: 5261
SfcI C'TryA_G
Cuts at: 33 1103 1202 1779 2304 2989 3982 4173 33
Size: 1070 99 577 525 685 993 191 1121
Fragments arranged by size:
1121 1070 993 685 577 525 191 99
SfoI GGC'GCC
Cuts at: 1650 1967 2693 2941 1650
Size: 317 726 248 3970
Fragments arranged by size:
3970 726 317 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5261
SmaI CCC'GGG
Cuts at: 1688 1760 2283 1688
Size: 72 523 4666
Fragments arranged by size:
4666 523 72
SmlI C'TyrA_G
Cuts at: 1408 2416 3331 3823 4085 1408
Size: 1008 915 492 262 2584
Fragments arranged by size:
2584 1008 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5261
SphI G_CATG'C
Cuts at: 571 2314 2814 571
Size: 1743 500 3018
Fragments arranged by size:
3018 1743 500
StyI C'CwwG_G
Cuts at: 107 194 1361 1699 1706 2132 2219 2395 2749
Size: 87 1167 338 7 426 87 176 354
Cuts at: 2749 107
Size: 2619
Fragments arranged by size:
2619 1167 426 354 338 176 87 87
7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 377 1757 2844 2977 3436 3645 377
Size: 1380 1087 133 459 209 1993
Fragments arranged by size:
1993 1380 1087 459 209 133
TatI w'GTAC_w
Cuts at: 4776 4776
Size: 5261
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1624 1656 4778 4810 1624
Size: 32 3122 32 2075
Fragments arranged by size:
3122 2075 32 32
Tth111I GACn'n_nGTC
Cuts at: 3058 3058
Size: 5261
XbaI T'CTAG_A
Cuts at: 2292 2292
Size: 5261
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 466 1633 2756 466
Size: 1167 1123 2971
Fragments arranged by size:
2971 1167 1123
XmaI C'CCGG_G
Cuts at: 1686 1758 2281 1686
Size: 72 523 4666
Fragments arranged by size:
4666 523 72
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AloI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsgI BsiWI BsmI BsrGI
BssHII BstZ17I Bsu36I ClaI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI