Restriction map for
pF1KB9777
(Circular) (Six-base) MAPSORT of: pF1KB9777.seq Check: 1049 from: 1 to: 5477
pF1KB9777 5477 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 2195 2515 2195
Size: 320 5157
Acc65I G'GTAC_C
Cuts at: 2493 2493
Size: 5477
AclI AA'CG_TT
Cuts at: 5317 5317
Size: 5477
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 584 979 1036 1609 3302 3734 4087 4749
Size: 469 395 57 573 1693 432 353 662
Cuts at: 4749 115
Size: 843
Fragments arranged by size:
1693 843 662 573 469 432 395 353
57
AflIII A'CryG_T
Cuts at: 2829 4629 4910 2829
Size: 1800 281 3396
Fragments arranged by size:
3396 1800 281
AleI CACnn'nnGTG
Cuts at: 677 1386 677
Size: 709 4768
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1831 1863 1831
Size: 32 5445
AlwNI CAG_nnn'CTG
Cuts at: 945 1434 2782 4220 945
Size: 489 1348 1438 2202
Fragments arranged by size:
2202 1438 1348 489
ApaI G_GGCC'C
Cuts at: 1409 1409
Size: 5477
ApaLI G'TGCA_C
Cuts at: 4315 4315
Size: 5477
ApoI r'AATT_y
Cuts at: 366 414 1231 2481 4830 5086 366
Size: 48 817 1250 2349 256 757
Fragments arranged by size:
2349 1250 817 757 256 48
AseI AT'TA_AT
Cuts at: 20 374 20
Size: 354 5123
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5477
AvaI C'yCGr_G
Cuts at: 202 913 1412 2081 2497 202
Size: 711 499 669 416 3182
Fragments arranged by size:
3182 711 669 499 416
BamHI G'GATC_C
Cuts at: 2110 2502 2110
Size: 392 5085
BanI G'GyrC_C
Cuts at: 1137 2493 2907 3155 3190 1137
Size: 1356 414 248 35 3424
Fragments arranged by size:
3424 1356 414 248 35
BanII G_rGCy'C
Cuts at: 410 1037 1409 2491 3521 410
Size: 627 372 1082 1030 2366
Fragments arranged by size:
2366 1082 1030 627 372
BbeI G_GCGC'C
Cuts at: 1141 2911 3159 1141
Size: 1770 248 3459
Fragments arranged by size:
3459 1770 248
BbsI GAAGACnn'nnnn_
Cuts at: 416 985 1384 1403 1597 416
Size: 569 399 19 194 4296
Fragments arranged by size:
4296 569 399 194 19
BciVI GTATCCnnnnn_n'
Cuts at: 3375 4431 5477 3375
Size: 1056 1046 3375
Fragments arranged by size:
3375 1056 1046
BclI T'GATC_A
Cuts at: 936 2997 936
Size: 2061 3416
BglII A'GATC_T
Cuts at: 2992 5029 2992
Size: 2037 3440
BlpI GC'TnA_GC
Cuts at: 1099 2589 1099
Size: 1490 3987
Bme1580I G_kGCm'C
Cuts at: 1409 3102 3195 4319 1409
Size: 1693 93 1124 2567
Fragments arranged by size:
2567 1693 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 849 1422 1637 2689 2770 3105 5249 849
Size: 573 215 1052 81 335 2144 1077
Fragments arranged by size:
2144 1077 1052 573 335 215 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3844 3844
Size: 5477
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 817 985 1456 1670 1849 2487 335
Size: 482 168 471 214 179 638 3325
Fragments arranged by size:
3325 638 482 471 214 179 168
Bpu10I CC'TnA_GC
Cuts at: 588 588
Size: 5477
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 2653 3532 4246 4544 394
Size: 2259 879 714 298 1327
Fragments arranged by size:
2259 1327 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1057 2132 5181 1057
Size: 1075 3049 1353
Fragments arranged by size:
3049 1353 1075
BsaAI yAC'GTr
Cuts at: 2830 2830
Size: 5477
BsaBI GATnn'nnATC
Cuts at: 6 2991 5028 6
Size: 2985 2037 455
Fragments arranged by size:
2985 2037 455
BsaHI Gr'CG_yC
Cuts at: 1138 2908 3156 3858 5354 1138
Size: 1770 248 702 1496 1261
Fragments arranged by size:
1770 1496 1261 702 248
BsaWI w'CCGG_w
Cuts at: 845 2428 2666 3187 3865 4276 4423 4697 845
Size: 1583 238 521 678 411 147 274 1625
Fragments arranged by size:
1625 1583 678 521 411 274 238 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 308 338 1663 1693 1831 1861 308
Size: 30 1325 30 138 30 3924
Fragments arranged by size:
3924 1325 138 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 538 1115 1936 2362 538
Size: 577 821 426 3653
Fragments arranged by size:
3653 821 577 426
BseYI C'CCAG_C
Cuts at: 1047 1141 1842 2902 3660 4325 4733 1047
Size: 94 701 1060 758 665 408 1791
Fragments arranged by size:
1791 1060 758 701 665 408 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 907
Size: 5477
BsiEI CG_ry'CG
Cuts at: 104 3019 3065 4295 104
Size: 2915 46 1230 1286
Fragments arranged by size:
2915 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 410 1037 1393 1684 1730 2491 3269 3459 4319
Size: 627 356 291 46 761 778 190 860
Cuts at: 4319 410
Size: 1568
Fragments arranged by size:
1568 860 778 761 627 356 291 190
46
BsmI GAATG_Cn'
Cuts at: 1058 1715 1058
Size: 657 4820
Bsp1286I G_dGCh'C
Cuts at: 410 1037 1393 1409 1684 1730 2491 3102 3195
Size: 627 356 16 275 46 761 611 93
Cuts at: 3195 3269 3459 3521 4319 410
Size: 74 190 62 798 1568
Fragments arranged by size:
1568 798 761 627 611 356 275 190
93 74 62 46 16
BspEI T'CCGG_A
Cuts at: 2428 2428
Size: 5477
BspHI T'CATG_A
Cuts at: 3909 3909
Size: 5477
BspMI ACCTGCnnnn'nnnn_
Cuts at: 747 1440 2527 2741 3043 3424 747
Size: 693 1087 214 302 381 2800
Fragments arranged by size:
2800 1087 693 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3769 3823 5307 5472 50
Size: 3719 54 1484 165 55
Fragments arranged by size:
3719 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 408 1944 3389 408
Size: 1536 1445 2496
Fragments arranged by size:
2496 1536 1445
BsrFI r'CCGG_y
Cuts at: 735 881 3475 3656 735
Size: 146 2594 181 2556
Fragments arranged by size:
2594 2556 181 146
BssSI C'ACGA_G
Cuts at: 1723 3748 4456 1723
Size: 2025 708 2744
Fragments arranged by size:
2744 2025 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2854 2854
Size: 5477
BstBI TT'CG_AA
Cuts at: 3838 3838
Size: 5477
BstEII G'GTnAC_C
Cuts at: 682 682
Size: 5477
BstXI CCAn_nnnn'nTGG
Cuts at: 963 963
Size: 5477
BstYI r'GATC_y
Cuts at: 1 1296 1584 2110 2425 2502 2969 2992 3327
Size: 1295 288 526 315 77 467 23 335
Cuts at: 3327 3573 3879 3891 3977 5029 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1295 1052 526 467 449 335 315 306
288 246 86 77 23 12
Bsu36I CC'TnA_GG
Cuts at: 215 512 1538 215
Size: 297 1026 4154
Fragments arranged by size:
4154 1026 297
BtgI C'CryG_G
Cuts at: 1133 1133
Size: 5477
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3427 3608 3771 3427
Size: 181 163 5133
Fragments arranged by size:
5133 181 163
BtsI GCAGTG_nn'
Cuts at: 684 717 2015 2720 684
Size: 33 1298 705 3441
Fragments arranged by size:
3441 1298 705 33
DraI TTT'AAA
Cuts at: 2268 2476 2268
Size: 208 5269
DrdI GACnn_nn'nnGTC
Cuts at: 846 3183 4527 846
Size: 2337 1344 1796
Fragments arranged by size:
2337 1796 1344
EaeI y'GGCC_r
Cuts at: 1797 2172 3062 3236 3627 3654 4999 1797
Size: 375 890 174 391 27 1345 2275
Fragments arranged by size:
2275 1345 890 391 375 174 27
EagI C'GGCC_G
Cuts at: 3062 3062
Size: 5477
EarI CTCTTCn'nnn_
Cuts at: 133 479 770 1012 1547 2339 3500 3710 133
Size: 346 291 242 535 792 1161 210 1900
Fragments arranged by size:
1900 1161 792 535 346 291 242 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1977 3685 4427 4573 5287 1977
Size: 1708 742 146 714 2167
Fragments arranged by size:
2167 1708 742 714 146
EcoICRI GAG'CTC
Cuts at: 408 1035 2489 408
Size: 627 1454 3396
Fragments arranged by size:
3396 1454 627
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 335 584 817 979 985 1036 1456 1609
Size: 220 249 233 162 6 51 420 153
Cuts at: 1609 1670 1849 2487 3302 3734 4087 4749 115
Size: 61 179 638 815 432 353 662 843
Fragments arranged by size:
843 815 662 638 432 420 353 249
233 220 179 162 153 61 51 6
EcoNI CCTnn'n_nnAGG
Cuts at: 440 440
Size: 5477
EcoO109I rG'GnC_Cy
Cuts at: 188 516 897 1405 2616 188
Size: 328 381 508 1211 3049
Fragments arranged by size:
3049 1211 508 381 328
EcoRI G'AATT_C
Cuts at: 414 2481 414
Size: 2067 3410
EcoRV GAT'ATC
Cuts at: 1974 1974
Size: 5477
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3496 3496
Size: 5477
FspI TGC'GCA
Cuts at: 3258 3258
Size: 5477
HaeII r_GCGC'y
Cuts at: 636 1141 1828 2911 3159 4389 5160 636
Size: 505 687 1083 248 1230 771 953
Fragments arranged by size:
1230 1083 953 771 687 505 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1668 1700 2359 2391 2410 2442 3319 3349 3351
Size: 32 659 32 19 32 877 30 2
Cuts at: 3351 3381 1668
Size: 30 3764
Fragments arranged by size:
3764 877 659 32 32 32 30 30
19 2
HincII GTy'rAC
Cuts at: 2196 2516 2196
Size: 320 5157
KasI G'GCGC_C
Cuts at: 1137 2907 3155 1137
Size: 1770 248 3459
Fragments arranged by size:
3459 1770 248
KpnI G_GTAC'C
Cuts at: 2497 2497
Size: 5477
MluI A'CGCG_T
Cuts at: 4910 4910
Size: 5477
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 220 439 1562 4236 4420 220
Size: 219 1123 2674 184 1277
Fragments arranged by size:
2674 1277 1123 219 184
MscI TGG'CCA
Cuts at: 1799 2174 3238 1799
Size: 375 1064 4038
Fragments arranged by size:
4038 1064 375
MslI CAynn'nnrTG
Cuts at: 263 677 1386 3466 3593 263
Size: 414 709 2080 127 2147
Fragments arranged by size:
2147 2080 709 414 127
MspA1I CmG'CkG
Cuts at: 963 1047 1179 2127 2588 2902 3262 4046 4291
Size: 84 132 948 461 314 360 784 245
Cuts at: 4291 963
Size: 2149
Fragments arranged by size:
2149 948 784 461 360 314 245 132
84
NaeI GCC'GGC
Cuts at: 737 883 3658 737
Size: 146 2775 2556
Fragments arranged by size:
2775 2556 146
NarI GG'CG_CC
Cuts at: 1138 2908 3156 1138
Size: 1770 248 3459
Fragments arranged by size:
3459 1770 248
NgoMIV G'CCGG_C
Cuts at: 735 881 3656 735
Size: 146 2775 2556
Fragments arranged by size:
2775 2556 146
NspI r_CATG'y
Cuts at: 2530 3030 4633 2530
Size: 500 1603 3374
Fragments arranged by size:
3374 1603 500
PasI CC'CwG_GG
Cuts at: 355 355
Size: 5477
PciI A'CATG_T
Cuts at: 4629 4629
Size: 5477
PflMI CCAn_nnn'nTGG
Cuts at: 1434 1742 1434
Size: 308 5169
PmeI GTTT'AAAC
Cuts at: 2476 2476
Size: 5477
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1831 1863 3923 3955 1831
Size: 32 2060 32 3353
Fragments arranged by size:
3353 2060 32 32
PpuMI rG'GwC_Cy
Cuts at: 188 516 897 188
Size: 328 381 4768
Fragments arranged by size:
4768 381 328
PspOMI G'GGCC_C
Cuts at: 1405 1405
Size: 5477
PspXI vC'TCGA_Gb
Cuts at: 913 913
Size: 5477
PstI C_TGCA'G
Cuts at: 276 758 1451 1820 2524 3209 276
Size: 482 693 369 704 685 2544
Fragments arranged by size:
2544 704 693 685 482 369
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5477
PvuII CAG'CTG
Cuts at: 963 1047 1179 2902 3262 963
Size: 84 132 1723 360 3178
Fragments arranged by size:
3178 1723 360 132 84
RsrII CG'GwC_CG
Cuts at: 3672 3672
Size: 5477
SacI G_AGCT'C
Cuts at: 410 1037 2491 410
Size: 627 1454 3396
Fragments arranged by size:
3396 1454 627
SalI G'TCGA_C
Cuts at: 2194 2514 2194
Size: 320 5157
SanDI GG'GwC_CC
Cuts at: 897 897
Size: 5477
SapI GCTCTTCn'nnn_
Cuts at: 770 1012 3500 3710 770
Size: 242 2488 210 2537
Fragments arranged by size:
2537 2488 242 210
SbfI CC_TGCA'GG
Cuts at: 2524 2524
Size: 5477
ScaI AGT'ACT
Cuts at: 4994 4994
Size: 5477
SfcI C'TryA_G
Cuts at: 33 272 754 1207 1447 1816 2520 3205 4173
Size: 239 482 453 240 369 704 685 968
Cuts at: 4173 4364 33
Size: 191 1146
Fragments arranged by size:
1146 968 704 685 482 453 369 240
239 191
SfoI GGC'GCC
Cuts at: 1139 2909 3157 1139
Size: 1770 248 3459
Fragments arranged by size:
3459 1770 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5477
SmaI CCC'GGG
Cuts at: 204 1414 2499 204
Size: 1210 1085 3182
Fragments arranged by size:
3182 1210 1085
SmlI C'TyrA_G
Cuts at: 409 913 2081 2632 3547 4261 4523 409
Size: 504 1168 551 915 714 262 1363
Fragments arranged by size:
1363 1168 915 714 551 504 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5477
SphI G_CATG'C
Cuts at: 2530 3030 2530
Size: 500 4977
SspI AAT'ATT
Cuts at: 151 151
Size: 5477
StuI AGG'CCT
Cuts at: 1991 1991
Size: 5477
StyI C'CwwG_G
Cuts at: 1076 1568 1736 2611 2965 1076
Size: 492 168 875 354 3588
Fragments arranged by size:
3588 875 492 354 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 275 3060 3193 3652 3861 275
Size: 2785 133 459 209 1891
Fragments arranged by size:
2785 1891 459 209 133
TatI w'GTAC_w
Cuts at: 534 1657 2186 2336 4992 534
Size: 1123 529 150 2656 1019
Fragments arranged by size:
2656 1123 1019 529 150
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 308 340 1663 1695 1737 1769 2102 2134 4994
Size: 32 1323 32 42 32 333 32 2860
Cuts at: 4994 5026 308
Size: 32 759
Fragments arranged by size:
2860 1323 759 333 42 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 169 1084 3274 169
Size: 915 2190 2372
Fragments arranged by size:
2372 2190 915
XbaI T'CTAG_A
Cuts at: 2508 2508
Size: 5477
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1176 2972 1176
Size: 1796 3681
XhoI C'TCGA_G
Cuts at: 913 2081 913
Size: 1168 4309
XmaI C'CCGG_G
Cuts at: 202 1412 2497 202
Size: 1210 1085 3182
Fragments arranged by size:
3182 1210 1085
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BsiWI BsmBI BsrGI BssHII BstZ17I ClaI CspCI DraIII
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PfoI PmlI PshAI PsiI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SwaI
XmnI ZraI