Restriction map for
pF1KB9779
(Circular) (Six-base) MAPSORT of: pF1KB9779.seq Check: 4994 from: 1 to: 5774
pF1KB9779 5774 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2540 2540
Size: 5774
AccI GT'mk_AC
Cuts at: 2812 2812
Size: 5774
Acc65I G'GTAC_C
Cuts at: 2790 2790
Size: 5774
AclI AA'CG_TT
Cuts at: 5614 5614
Size: 5774
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1445 2201 3599 4031 4384 5046 1445
Size: 756 1398 432 353 662 2173
Fragments arranged by size:
2173 1398 756 662 432 353
AflII C'TTAA_G
Cuts at: 169 169
Size: 5774
AflIII A'CryG_T
Cuts at: 3126 4926 5207 3126
Size: 1800 281 3693
Fragments arranged by size:
3693 1800 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2551 2583 2551
Size: 32 5742
AlwNI CAG_nnn'CTG
Cuts at: 469 2173 2691 3079 4517 469
Size: 1704 518 388 1438 1726
Fragments arranged by size:
1726 1704 1438 518 388
ApaLI G'TGCA_C
Cuts at: 4612 4612
Size: 5774
ApoI r'AATT_y
Cuts at: 766 897 1102 1326 1401 1413 1857 2641 2778
Size: 131 205 224 75 12 444 784 137
Cuts at: 2778 5127 5383 766
Size: 2349 256 1157
Fragments arranged by size:
2349 1157 784 444 256 224 205 137
131 75 12
AseI AT'TA_AT
Cuts at: 20 20
Size: 5774
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5774
AvaI C'yCGr_G
Cuts at: 2794 2794
Size: 5774
BamHI G'GATC_C
Cuts at: 2412 2799 2412
Size: 387 5387
BanI G'GyrC_C
Cuts at: 2339 2790 3204 3452 3487 2339
Size: 451 414 248 35 4626
Fragments arranged by size:
4626 451 414 248 35
BanII G_rGCy'C
Cuts at: 2788 3818 2788
Size: 1030 4744
BbeI G_GCGC'C
Cuts at: 3208 3456 3208
Size: 248 5526
BbsI GAAGACnn'nnnn_
Cuts at: 233 233
Size: 5774
BbvCI CC'TCA_GC
Cuts at: 2264 2264
Size: 5774
BciVI GTATCCnnnnn_n'
Cuts at: 3672 4728 5774 3672
Size: 1056 1046 3672
Fragments arranged by size:
3672 1056 1046
BclI T'GATC_A
Cuts at: 2309 3294 2309
Size: 985 4789
BfrBI ATG'CAT
Cuts at: 528 528
Size: 5774
BglI GCCn_nnn'nGGC
Cuts at: 424 1147 424
Size: 723 5051
BglII A'GATC_T
Cuts at: 1047 1527 3289 5326 1047
Size: 480 1762 2037 1495
Fragments arranged by size:
2037 1762 1495 480
BlpI GC'TnA_GC
Cuts at: 2886 2886
Size: 5774
Bme1580I G_kGCm'C
Cuts at: 1953 2476 3399 3492 4616 1953
Size: 523 923 93 1124 3111
Fragments arranged by size:
3111 1124 923 523 93
BmrI ACTGGGnnnn_n'
Cuts at: 2748 2986 3067 3402 5546 2748
Size: 238 81 335 2144 2976
Fragments arranged by size:
2976 2144 335 238 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 285 4141 285
Size: 3856 1918
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2781 2781
Size: 5774
Bpu10I CC'TnA_GC
Cuts at: 2264 2264
Size: 5774
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1118 2950 3829 4543 4841 1118
Size: 1832 879 714 298 2051
Fragments arranged by size:
2051 1832 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5478 5478
Size: 5774
BsaAI yAC'GTr
Cuts at: 3127 3127
Size: 5774
BsaBI GATnn'nnATC
Cuts at: 6 3288 5325 6
Size: 3282 2037 455
Fragments arranged by size:
3282 2037 455
BsaHI Gr'CG_yC
Cuts at: 3205 3453 4155 5651 3205
Size: 248 702 1496 3328
Fragments arranged by size:
3328 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1923 2963 3484 4162 4573 4720 4994 1923
Size: 1040 521 678 411 147 274 2703
Fragments arranged by size:
2703 1040 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 567 597 567
Size: 30 5744
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1466 1466
Size: 5774
BseYI C'CCAG_C
Cuts at: 3199 3957 4622 5030 3199
Size: 758 665 408 3943
Fragments arranged by size:
3943 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1721 1946 1721
Size: 225 5549
BsiEI CG_ry'CG
Cuts at: 104 3316 3362 4592 104
Size: 3212 46 1230 1286
Fragments arranged by size:
3212 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2788 3566 3756 4616 2788
Size: 778 190 860 3946
Fragments arranged by size:
3946 860 778 190
BsmI GAATG_Cn'
Cuts at: 420 2204 420
Size: 1784 3990
Bsp1286I G_dGCh'C
Cuts at: 1953 2476 2788 3399 3492 3566 3756 3818 4616
Size: 523 312 611 93 74 190 62 798
Cuts at: 4616 1953
Size: 3111
Fragments arranged by size:
3111 798 611 523 312 190 93 74
62
BspEI T'CCGG_A
Cuts at: 1923 1923
Size: 5774
BspHI T'CATG_A
Cuts at: 4206 4206
Size: 5774
BspMI ACCTGCnnnn'nnnn_
Cuts at: 413 2540 2824 3038 3340 3721 413
Size: 2127 284 214 302 381 2466
Fragments arranged by size:
2466 2127 381 302 284 214
BsrBI CCG'CTC
Cuts at: 50 4066 4120 5604 5769 50
Size: 4016 54 1484 165 55
Fragments arranged by size:
4016 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1831 3686 1831
Size: 1855 3919
BsrFI r'CCGG_y
Cuts at: 3772 3953 3772
Size: 181 5593
BssSI C'ACGA_G
Cuts at: 981 4045 4753 981
Size: 3064 708 2002
Fragments arranged by size:
3064 2002 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2682 3151 2682
Size: 469 5305
BstBI TT'CG_AA
Cuts at: 4135 4135
Size: 5774
BstYI r'GATC_y
Cuts at: 1 1047 1125 1527 1635 2412 2799 3266 3289
Size: 1046 78 402 108 777 387 467 23
Cuts at: 3289 3624 3870 4176 4188 4274 5326 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 1046 777 467 449 402 387 335
306 246 108 86 78 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5774
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3724 3905 4068 3724
Size: 181 163 5430
Fragments arranged by size:
5430 181 163
BtsI GCAGTG_nn'
Cuts at: 470 2732 3017 470
Size: 2262 285 3227
Fragments arranged by size:
3227 2262 285
DraI TTT'AAA
Cuts at: 1944 2773 1944
Size: 829 4945
DraIII CAC_nnn'GTG
Cuts at: 669 2236 2482 669
Size: 1567 246 3961
Fragments arranged by size:
3961 1567 246
DrdI GACnn_nn'nnGTC
Cuts at: 538 3480 4824 538
Size: 2942 1344 1488
Fragments arranged by size:
2942 1488 1344
EaeI y'GGCC_r
Cuts at: 3359 3533 3924 3951 5296 3359
Size: 174 391 27 1345 3837
Fragments arranged by size:
3837 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3359 3359
Size: 5774
EarI CTCTTCn'nnn_
Cuts at: 187 1473 2383 2429 2660 3797 4007 187
Size: 1286 910 46 231 1137 210 1954
Fragments arranged by size:
1954 1286 1137 910 231 210 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3982 4724 4870 5584 3982
Size: 742 146 714 4172
Fragments arranged by size:
4172 742 714 146
EcoICRI GAG'CTC
Cuts at: 2786 2786
Size: 5774
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1445 2201 2781 3599 4031 4384 5046 1445
Size: 756 580 818 432 353 662 2173
Fragments arranged by size:
2173 818 756 662 580 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 473 473
Size: 5774
EcoO109I rG'GnC_Cy
Cuts at: 2913 2913
Size: 5774
EcoRI G'AATT_C
Cuts at: 2778 2778
Size: 5774
EcoRV GAT'ATC
Cuts at: 156 1547 156
Size: 1391 4383
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 965 2059 3793 965
Size: 1094 1734 2946
Fragments arranged by size:
2946 1734 1094
FspI TGC'GCA
Cuts at: 3555 3555
Size: 5774
HaeII r_GCGC'y
Cuts at: 3208 3456 4686 5457 3208
Size: 248 1230 771 3525
Fragments arranged by size:
3525 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 612 644 820 852 1192 1224 3616 3646 3648
Size: 32 176 32 340 32 2392 30 2
Cuts at: 3648 3678 612
Size: 30 2708
Fragments arranged by size:
2708 2392 340 176 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2813 2813
Size: 5774
HindIII A'AGCT_T
Cuts at: 180 922 180
Size: 742 5032
KasI G'GCGC_C
Cuts at: 3204 3452 3204
Size: 248 5526
KpnI G_GTAC'C
Cuts at: 2794 2794
Size: 5774
MfeI C'AATT_G
Cuts at: 1300 1300
Size: 5774
MluI A'CGCG_T
Cuts at: 5207 5207
Size: 5774
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2310 2639 4533 4717 2310
Size: 329 1894 184 3367
Fragments arranged by size:
3367 1894 329 184
MscI TGG'CCA
Cuts at: 3535 3535
Size: 5774
MslI CAynn'nnrTG
Cuts at: 2021 3763 3890 2021
Size: 1742 127 3905
Fragments arranged by size:
3905 1742 127
MspA1I CmG'CkG
Cuts at: 2885 3199 3559 4343 4588 2885
Size: 314 360 784 245 4071
Fragments arranged by size:
4071 784 360 314 245
NaeI GCC'GGC
Cuts at: 3955 3955
Size: 5774
NarI GG'CG_CC
Cuts at: 3205 3453 3205
Size: 248 5526
NcoI C'CATG_G
Cuts at: 107 107
Size: 5774
NdeI CA'TA_TG
Cuts at: 436 1035 436
Size: 599 5175
NgoMIV G'CCGG_C
Cuts at: 3953 3953
Size: 5774
NsiI A_TGCA'T
Cuts at: 530 530
Size: 5774
NspI r_CATG'y
Cuts at: 269 513 2685 2827 3327 4930 269
Size: 244 2172 142 500 1603 1113
Fragments arranged by size:
2172 1603 1113 500 244 142
PciI A'CATG_T
Cuts at: 4926 4926
Size: 5774
PfoI T'CCnGG_A
Cuts at: 221 453 221
Size: 232 5542
PmeI GTTT'AAAC
Cuts at: 2773 2773
Size: 5774
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1514 1546 4220 4252 1514
Size: 32 2674 32 3036
Fragments arranged by size:
3036 2674 32 32
PsiI TTA'TAA
Cuts at: 212 1444 2117 212
Size: 1232 673 3869
Fragments arranged by size:
3869 1232 673
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2482 2514 2482
Size: 32 5742
PstI C_TGCA'G
Cuts at: 1422 2727 2821 3506 1422
Size: 1305 94 685 3690
Fragments arranged by size:
3690 1305 685 94
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5774
PvuII CAG'CTG
Cuts at: 3199 3559 3199
Size: 360 5414
RsrII CG'GwC_CG
Cuts at: 3969 3969
Size: 5774
SacI G_AGCT'C
Cuts at: 2788 2788
Size: 5774
SalI G'TCGA_C
Cuts at: 2811 2811
Size: 5774
SapI GCTCTTCn'nnn_
Cuts at: 3797 4007 3797
Size: 210 5564
SbfI CC_TGCA'GG
Cuts at: 2821 2821
Size: 5774
ScaI AGT'ACT
Cuts at: 2758 5291 2758
Size: 2533 3241
SfcI C'TryA_G
Cuts at: 33 138 470 1418 1825 2723 2817 3502 4470
Size: 105 332 948 407 898 94 685 968
Cuts at: 4470 4661 33
Size: 191 1146
Fragments arranged by size:
1146 968 948 898 685 407 332 191
105 94
SfiI GGCCn_nnn'nGGCC
Cuts at: 1147 1147
Size: 5774
SfoI GGC'GCC
Cuts at: 3206 3454 3206
Size: 248 5526
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5774
SmaI CCC'GGG
Cuts at: 2796 2796
Size: 5774
SmlI C'TyrA_G
Cuts at: 169 1133 2929 3844 4558 4820 169
Size: 964 1796 915 714 262 1123
Fragments arranged by size:
1796 1123 964 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5774
SphI G_CATG'C
Cuts at: 2827 3327 2827
Size: 500 5274
SspI AAT'ATT
Cuts at: 999 999
Size: 5774
StuI AGG'CCT
Cuts at: 1150 1150
Size: 5774
StyI C'CwwG_G
Cuts at: 107 519 1151 1196 2908 3262 107
Size: 412 632 45 1712 354 2619
Fragments arranged by size:
2619 1712 632 412 354 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3357 3490 3949 4158 3357
Size: 133 459 209 4973
Fragments arranged by size:
4973 459 209 133
TatI w'GTAC_w
Cuts at: 352 1655 1782 2134 2756 5289 352
Size: 1303 127 352 622 2533 837
Fragments arranged by size:
2533 1303 837 622 352 127
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 449 481 567 599 653 685 2291 2323 5291
Size: 32 86 32 54 32 1606 32 2968
Cuts at: 5291 5323 449
Size: 32 900
Fragments arranged by size:
2968 1606 900 86 54 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3571 3571
Size: 5774
XbaI T'CTAG_A
Cuts at: 2805 2805
Size: 5774
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1149 2389 3269 1149
Size: 1240 880 3654
Fragments arranged by size:
3654 1240 880
XmaI C'CCGG_G
Cuts at: 2794 2794
Size: 5774
XmnI GAAnn'nnTTC
Cuts at: 1413 2541 2738 1413
Size: 1128 197 4449
Fragments arranged by size:
4449 1128 197
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PfoI PmeI PpiI
PsiI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AleI AlfI ApaI AscI
AvrII BaeI BcgI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI FseI
FspAI HpaI NheI NotI NruI PacI PasI PflMI
PmlI PpuMI PshAI PspOMI PspXI SacII SanDI SexAI
SgrAI SnaBI SrfI SwaI XhoI ZraI