Restriction map for
pF1KB9787
(Circular) (Six-base) MAPSORT of: pF1KB9787.seq Check: 8204 from: 1 to: 3686
pF1KB9787 3686 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 579 579
Size: 3686
AatII G_ACGT'C
Cuts at: 242 242
Size: 3686
AccI GT'mk_AC
Cuts at: 724 724
Size: 3686
Acc65I G'GTAC_C
Cuts at: 702 702
Size: 3686
AclI AA'CG_TT
Cuts at: 3526 3526
Size: 3686
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 1511 1943 2690 2958 285
Size: 1226 432 747 268 1013
Fragments arranged by size:
1226 1013 747 432 268
AflIII A'CryG_T
Cuts at: 1038 2142 3119 1038
Size: 1104 977 1605
Fragments arranged by size:
1605 1104 977
AgeI A'CCGG_T
Cuts at: 2074 2074
Size: 3686
AleI CACnn'nnGTG
Cuts at: 232 232
Size: 3686
AlwNI CAG_nnn'CTG
Cuts at: 991 2558 991
Size: 1567 2119
ApaI G_GGCC'C
Cuts at: 482 482
Size: 3686
ApaLI G'TGCA_C
Cuts at: 2456 2456
Size: 3686
ApoI r'AATT_y
Cuts at: 690 3039 3295 690
Size: 2349 256 1081
Fragments arranged by size:
2349 1081 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3686
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3686
AvaI C'yCGr_G
Cuts at: 706 706
Size: 3686
BamHI G'GATC_C
Cuts at: 711 711
Size: 3686
BanI G'GyrC_C
Cuts at: 702 1116 1364 1399 702
Size: 414 248 35 2989
Fragments arranged by size:
2989 414 248 35
BanII G_rGCy'C
Cuts at: 482 700 1730 482
Size: 218 1030 2438
Fragments arranged by size:
2438 1030 218
BbeI G_GCGC'C
Cuts at: 1120 1368 1120
Size: 248 3438
BbsI GAAGACnn'nnnn_
Cuts at: 234 234
Size: 3686
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 660 694 90
Size: 34 536 34 3082
Fragments arranged by size:
3082 536 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1584 2345 3686 1584
Size: 761 1341 1584
Fragments arranged by size:
1584 1341 761
BclI T'GATC_A
Cuts at: 1206 1206
Size: 3686
BglII A'GATC_T
Cuts at: 674 1201 3238 674
Size: 527 2037 1122
Fragments arranged by size:
2037 1122 527
BlpI GC'TnA_GC
Cuts at: 561 798 561
Size: 237 3449
Bme1580I G_kGCm'C
Cuts at: 482 1311 1404 2460 482
Size: 829 93 1056 1708
Fragments arranged by size:
1708 1056 829 93
BmgBI CAC'GTC
Cuts at: 339 339
Size: 3686
BmrI ACTGGGnnnn_n'
Cuts at: 898 979 1314 3458 898
Size: 81 335 2144 1126
Fragments arranged by size:
2144 1126 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2053 2053
Size: 3686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 574
Size: 3686
Bpu10I CC'TnA_GC
Cuts at: 568 568
Size: 3686
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 862 1741 2233 2531 862
Size: 879 492 298 2017
Fragments arranged by size:
2017 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 181 3390 181
Size: 3209 477
BsaAI yAC'GTr
Cuts at: 1039 1039
Size: 3686
BsaBI GATnn'nnATC
Cuts at: 6 1200 3237 6
Size: 1194 2037 455
Fragments arranged by size:
2037 1194 455
BsaHI Gr'CG_yC
Cuts at: 239 296 1117 1365 2067 3563 239
Size: 57 821 248 702 1496 362
Fragments arranged by size:
1496 821 702 362 248 57
BsaWI w'CCGG_w
Cuts at: 875 1396 2074 2348 2495 2906 875
Size: 521 678 274 147 411 1655
Fragments arranged by size:
1655 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 475 505 475
Size: 30 3656
BseYI C'CCAG_C
Cuts at: 1111 1869 2446 2942 1111
Size: 758 577 496 1855
Fragments arranged by size:
1855 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1228 1274 2482 104
Size: 1124 46 1208 1308
Fragments arranged by size:
1308 1208 1124 46
BsiHKAI G_wGCw'C
Cuts at: 562 700 1478 1668 2460 562
Size: 138 778 190 792 1788
Fragments arranged by size:
1788 792 778 190 138
BsmBI CGTCTCn'nnnn_
Cuts at: 627 627
Size: 3686
Bsp1286I G_dGCh'C
Cuts at: 482 562 700 1311 1404 1478 1668 1730 2460
Size: 80 138 611 93 74 190 62 730
Cuts at: 2460 482
Size: 1708
Fragments arranged by size:
1708 730 611 190 138 93 80 74
62
BspEI T'CCGG_A
Cuts at: 2906 2906
Size: 3686
BspHI T'CATG_A
Cuts at: 2862 2862
Size: 3686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 579 736 950 1252 1633 579
Size: 157 214 302 381 2632
Fragments arranged by size:
2632 381 302 214 157
BsrBI CCG'CTC
Cuts at: 50 1978 2032 3516 3681 50
Size: 1928 54 1484 165 55
Fragments arranged by size:
1928 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 297 1598 297
Size: 1301 2385
BsrFI r'CCGG_y
Cuts at: 286 1684 1865 2074 286
Size: 1398 181 209 1898
Fragments arranged by size:
1898 1398 209 181
BssSI C'ACGA_G
Cuts at: 1957 2315 1957
Size: 358 3328
BstAPI GCAn_nnn'nTGC
Cuts at: 1063 1063
Size: 3686
BstBI TT'CG_AA
Cuts at: 2047 2047
Size: 3686
BstYI r'GATC_y
Cuts at: 1 674 711 1178 1201 1536 1782 2794 2880
Size: 673 37 467 23 335 246 1012 86
Cuts at: 2880 2892 3238 1
Size: 12 346 449
Fragments arranged by size:
1012 673 467 449 346 335 246 86
37 23 12
BtgI C'CryG_G
Cuts at: 506 506
Size: 3686
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1636 1817 1980 1636
Size: 181 163 3342
Fragments arranged by size:
3342 181 163
BtsI GCAGTG_nn'
Cuts at: 929 929
Size: 3686
DraI TTT'AAA
Cuts at: 685 685
Size: 3686
DrdI GACnn_nn'nnGTC
Cuts at: 1392 2250 1392
Size: 858 2828
EaeI y'GGCC_r
Cuts at: 362 449 1271 1445 1836 1863 3208 362
Size: 87 822 174 391 27 1345 840
Fragments arranged by size:
1345 840 822 391 174 87 27
EagI C'GGCC_G
Cuts at: 1271 1271
Size: 3686
EarI CTCTTCn'nnn_
Cuts at: 658 1709 1919 658
Size: 1051 210 2425
Fragments arranged by size:
2425 1051 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1894 2204 2350 3496 1894
Size: 310 146 1146 2084
Fragments arranged by size:
2084 1146 310 146
EcoICRI GAG'CTC
Cuts at: 698 698
Size: 3686
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 574 1511 1943 2690 2958 285
Size: 289 937 432 747 268 1013
Fragments arranged by size:
1013 937 747 432 289 268
EcoO109I rG'GnC_Cy
Cuts at: 478 479 488 825 478
Size: 1 9 337 3339
Fragments arranged by size:
3339 337 9 1
EcoRI G'AATT_C
Cuts at: 690 690
Size: 3686
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1705 1705
Size: 3686
FspI TGC'GCA
Cuts at: 1467 1467
Size: 3686
HaeII r_GCGC'y
Cuts at: 1120 1368 2390 3369 1120
Size: 248 1022 979 1437
Fragments arranged by size:
1437 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1528 1558 1560 1590 1528
Size: 30 2 30 3624
Fragments arranged by size:
3624 30 30 2
HincII GTy'rAC
Cuts at: 621 725 621
Size: 104 3582
KasI G'GCGC_C
Cuts at: 1116 1364 1116
Size: 248 3438
KpnI G_GTAC'C
Cuts at: 706 706
Size: 3686
MluI A'CGCG_T
Cuts at: 3119 3119
Size: 3686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2357 2541 2357
Size: 184 3502
MscI TGG'CCA
Cuts at: 364 451 1447 364
Size: 87 996 2603
Fragments arranged by size:
2603 996 87
MslI CAynn'nnrTG
Cuts at: 232 1675 1802 232
Size: 1443 127 2116
Fragments arranged by size:
2116 1443 127
MspA1I CmG'CkG
Cuts at: 468 797 1111 1471 2484 2729 468
Size: 329 314 360 1013 245 1425
Fragments arranged by size:
1425 1013 360 329 314 245
NaeI GCC'GGC
Cuts at: 288 1867 288
Size: 1579 2107
NarI GG'CG_CC
Cuts at: 1117 1365 1117
Size: 248 3438
NcoI C'CATG_G
Cuts at: 506 506
Size: 3686
NgoMIV G'CCGG_C
Cuts at: 286 1865 286
Size: 1579 2107
NspI r_CATG'y
Cuts at: 739 1239 2146 739
Size: 500 907 2279
Fragments arranged by size:
2279 907 500
PciI A'CATG_T
Cuts at: 2142 2142
Size: 3686
PfoI T'CCnGG_A
Cuts at: 194 194
Size: 3686
PmeI GTTT'AAAC
Cuts at: 685 685
Size: 3686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2825 2857 2825
Size: 32 3654
PspOMI G'GGCC_C
Cuts at: 478 478
Size: 3686
PstI C_TGCA'G
Cuts at: 733 1418 733
Size: 685 3001
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3686
PvuII CAG'CTG
Cuts at: 1111 1471 1111
Size: 360 3326
RsrII CG'GwC_CG
Cuts at: 1881 1881
Size: 3686
SacI G_AGCT'C
Cuts at: 700 700
Size: 3686
SalI G'TCGA_C
Cuts at: 723 723
Size: 3686
SapI GCTCTTCn'nnn_
Cuts at: 658 1709 1919 658
Size: 1051 210 2425
Fragments arranged by size:
2425 1051 210
SbfI CC_TGCA'GG
Cuts at: 733 733
Size: 3686
ScaI AGT'ACT
Cuts at: 3203 3203
Size: 3686
SfcI C'TryA_G
Cuts at: 33 729 1414 2407 2598 33
Size: 696 685 993 191 1121
Fragments arranged by size:
1121 993 696 685 191
SfoI GGC'GCC
Cuts at: 1118 1366 1118
Size: 248 3438
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3686
SmaI CCC'GGG
Cuts at: 708 708
Size: 3686
SmlI C'TyrA_G
Cuts at: 841 1756 2248 2510 841
Size: 915 492 262 2017
Fragments arranged by size:
2017 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3686
SphI G_CATG'C
Cuts at: 739 1239 739
Size: 500 3186
StuI AGG'CCT
Cuts at: 202 202
Size: 3686
StyI C'CwwG_G
Cuts at: 506 542 820 1174 506
Size: 36 278 354 3018
Fragments arranged by size:
3018 354 278 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 469 1269 1402 1861 2070 469
Size: 800 133 459 209 2085
Fragments arranged by size:
2085 800 459 209 133
TatI w'GTAC_w
Cuts at: 582 3201 582
Size: 2619 1067
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 475 507 3203 3235 475
Size: 32 2696 32 926
Fragments arranged by size:
2696 926 32 32
Tth111I GACn'n_nGTC
Cuts at: 1483 1483
Size: 3686
XbaI T'CTAG_A
Cuts at: 717 717
Size: 3686
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 549 1181 549
Size: 632 3054
XmaI C'CCGG_G
Cuts at: 706 706
Size: 3686
ZraI GAC'GTC
Cuts at: 240 240
Size: 3686
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI AscI AvrII BaeI
BbvCI BfrBI BglI BmtI BseRI BsgI BsiWI BsmI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI