Restriction map for
pF1KB9789
(Circular) (Six-base) MAPSORT of: pF1KB9789.seq Check: 9452 from: 1 to: 3719
pF1KB9789 3719 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 757 757
Size: 3719
Acc65I G'GTAC_C
Cuts at: 735 735
Size: 3719
AclI AA'CG_TT
Cuts at: 3559 3559
Size: 3719
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1544 1976 2723 2991 1544
Size: 432 747 268 2272
Fragments arranged by size:
2272 747 432 268
AfeI AGC'GCT
Cuts at: 515 515
Size: 3719
AflII C'TTAA_G
Cuts at: 517 517
Size: 3719
AflIII A'CryG_T
Cuts at: 1071 2175 3152 1071
Size: 1104 977 1638
Fragments arranged by size:
1638 1104 977
AgeI A'CCGG_T
Cuts at: 2107 2107
Size: 3719
AleI CACnn'nnGTG
Cuts at: 396 396
Size: 3719
AlwNI CAG_nnn'CTG
Cuts at: 465 1024 2591 465
Size: 559 1567 1593
Fragments arranged by size:
1593 1567 559
ApaI G_GGCC'C
Cuts at: 556 632 556
Size: 76 3643
ApaLI G'TGCA_C
Cuts at: 2489 2489
Size: 3719
ApoI r'AATT_y
Cuts at: 723 3072 3328 723
Size: 2349 256 1114
Fragments arranged by size:
2349 1114 256
AscI GG'CGCG_CC
Cuts at: 208 384 208
Size: 176 3543
AseI AT'TA_AT
Cuts at: 20 20
Size: 3719
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3719
AvaI C'yCGr_G
Cuts at: 425 604 739 425
Size: 179 135 3405
Fragments arranged by size:
3405 179 135
AvrII C'CTAG_G
Cuts at: 639 639
Size: 3719
BamHI G'GATC_C
Cuts at: 744 744
Size: 3719
BanI G'GyrC_C
Cuts at: 185 574 735 1149 1397 1432 185
Size: 389 161 414 248 35 2472
Fragments arranged by size:
2472 414 389 248 161 35
BanII G_rGCy'C
Cuts at: 296 527 556 632 733 1763 296
Size: 231 29 76 101 1030 2252
Fragments arranged by size:
2252 1030 231 101 76 29
BbeI G_GCGC'C
Cuts at: 189 578 1153 1401 189
Size: 389 575 248 2507
Fragments arranged by size:
2507 575 389 248
BciVI GTATCCnnnnn_n'
Cuts at: 1617 2378 3719 1617
Size: 761 1341 1617
Fragments arranged by size:
1617 1341 761
BclI T'GATC_A
Cuts at: 1239 1239
Size: 3719
BglI GCCn_nnn'nGGC
Cuts at: 112 278 560 572 112
Size: 166 282 12 3259
Fragments arranged by size:
3259 282 166 12
BglII A'GATC_T
Cuts at: 1234 3271 1234
Size: 2037 1682
BlpI GC'TnA_GC
Cuts at: 831 831
Size: 3719
Bme1580I G_kGCm'C
Cuts at: 556 632 1344 1437 2493 556
Size: 76 712 93 1056 1782
Fragments arranged by size:
1782 1056 712 93 76
BmrI ACTGGGnnnn_n'
Cuts at: 931 1012 1347 3491 931
Size: 81 335 2144 1159
Fragments arranged by size:
2144 1159 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 3719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 496 639 151
Size: 345 143 3231
Fragments arranged by size:
3231 345 143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 895 1774 2266 2564 280
Size: 615 879 492 298 1435
Fragments arranged by size:
1435 879 615 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3423 3423
Size: 3719
BsaAI yAC'GTr
Cuts at: 1072 1072
Size: 3719
BsaBI GATnn'nnATC
Cuts at: 6 1233 3270 6
Size: 1227 2037 455
Fragments arranged by size:
2037 1227 455
BsaHI Gr'CG_yC
Cuts at: 186 312 575 1150 1398 2100 3596 186
Size: 126 263 575 248 702 1496 309
Fragments arranged by size:
1496 702 575 309 263 248 126
BsaWI w'CCGG_w
Cuts at: 678 908 1429 2107 2381 2528 2939 678
Size: 230 521 678 274 147 411 1458
Fragments arranged by size:
1458 678 521 411 274 230 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 148 163 214 505 148
Size: 15 51 291 3362
Fragments arranged by size:
3362 291 51 15
BseYI C'CCAG_C
Cuts at: 537 568 1144 1902 2479 2975 537
Size: 31 576 758 577 496 1281
Fragments arranged by size:
1281 758 577 576 496 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 247
Size: 3719
BsiEI CG_ry'CG
Cuts at: 104 237 1261 1307 2515 104
Size: 133 1024 46 1208 1308
Fragments arranged by size:
1308 1208 1024 133 46
BsiHKAI G_wGCw'C
Cuts at: 296 527 733 1511 1701 2493 296
Size: 231 206 778 190 792 1522
Fragments arranged by size:
1522 792 778 231 206 190
Bsp1286I G_dGCh'C
Cuts at: 296 527 556 632 733 1344 1437 1511 1701
Size: 231 29 76 101 611 93 74 190
Cuts at: 1701 1763 2493 296
Size: 62 730 1522
Fragments arranged by size:
1522 730 611 231 190 101 93 76
74 62 29
BspEI T'CCGG_A
Cuts at: 678 2939 678
Size: 2261 1458
BspHI T'CATG_A
Cuts at: 2895 2895
Size: 3719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 769 983 1285 1666 769
Size: 214 302 381 2822
Fragments arranged by size:
2822 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2011 2065 3549 3714 50
Size: 1961 54 1484 165 55
Fragments arranged by size:
1961 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1631 1631
Size: 3719
BsrFI r'CCGG_y
Cuts at: 308 1717 1898 2107 308
Size: 1409 181 209 1920
Fragments arranged by size:
1920 1409 209 181
BssHII G'CGCG_C
Cuts at: 208 384 208
Size: 176 3543
BssSI C'ACGA_G
Cuts at: 1990 2348 1990
Size: 358 3361
BstAPI GCAn_nnn'nTGC
Cuts at: 1096 1096
Size: 3719
BstBI TT'CG_AA
Cuts at: 2080 2080
Size: 3719
BstYI r'GATC_y
Cuts at: 1 284 744 1211 1234 1569 1815 2827 2913
Size: 283 460 467 23 335 246 1012 86
Cuts at: 2913 2925 3271 1
Size: 12 346 449
Fragments arranged by size:
1012 467 460 449 346 335 283 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 558 107
Size: 451 3268
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 659 1669 1850 2013 659
Size: 1010 181 163 2365
Fragments arranged by size:
2365 1010 181 163
BtsI GCAGTG_nn'
Cuts at: 962 962
Size: 3719
DraI TTT'AAA
Cuts at: 718 718
Size: 3719
DrdI GACnn_nn'nnGTC
Cuts at: 1425 2283 1425
Size: 858 2861
EaeI y'GGCC_r
Cuts at: 234 467 1304 1478 1869 1896 3241 234
Size: 233 837 174 391 27 1345 712
Fragments arranged by size:
1345 837 712 391 233 174 27
EagI C'GGCC_G
Cuts at: 234 1304 234
Size: 1070 2649
EarI CTCTTCn'nnn_
Cuts at: 167 412 1742 1952 167
Size: 245 1330 210 1934
Fragments arranged by size:
1934 1330 245 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1927 2237 2383 3529 1927
Size: 310 146 1146 2117
Fragments arranged by size:
2117 1146 310 146
EcoICRI GAG'CTC
Cuts at: 294 525 731 294
Size: 231 206 3282
Fragments arranged by size:
3282 231 206
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 496 639 1544 1976 2723 2991 151
Size: 345 143 905 432 747 268 879
Fragments arranged by size:
905 879 747 432 345 268 143
EcoNI CCTnn'n_nnAGG
Cuts at: 244 244
Size: 3719
EcoO109I rG'GnC_Cy
Cuts at: 413 441 552 553 629 858 413
Size: 28 111 1 76 229 3274
Fragments arranged by size:
3274 229 111 76 28 1
EcoRI G'AATT_C
Cuts at: 723 723
Size: 3719
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1738 1738
Size: 3719
FspI TGC'GCA
Cuts at: 1500 1500
Size: 3719
HaeII r_GCGC'y
Cuts at: 163 189 372 517 538 578 1153 1401 2423
Size: 26 183 145 21 40 575 248 1022
Cuts at: 2423 3402 163
Size: 979 480
Fragments arranged by size:
1022 979 575 480 248 183 145 40
26 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 637 669 1561 1591 1593 1623 637
Size: 32 892 30 2 30 2733
Fragments arranged by size:
2733 892 32 30 30 2
HincII GTy'rAC
Cuts at: 758 758
Size: 3719
KasI G'GCGC_C
Cuts at: 185 574 1149 1397 185
Size: 389 575 248 2507
Fragments arranged by size:
2507 575 389 248
KpnI G_GTAC'C
Cuts at: 739 739
Size: 3719
MluI A'CGCG_T
Cuts at: 3152 3152
Size: 3719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2390 2574 2390
Size: 184 3535
MscI TGG'CCA
Cuts at: 1480 1480
Size: 3719
MslI CAynn'nnrTG
Cuts at: 396 1708 1835 396
Size: 1312 127 2280
Fragments arranged by size:
2280 1312 127
MspA1I CmG'CkG
Cuts at: 264 560 572 830 1144 1504 2517 2762 264
Size: 296 12 258 314 360 1013 245 1221
Fragments arranged by size:
1221 1013 360 314 296 258 245 12
NaeI GCC'GGC
Cuts at: 1900 1900
Size: 3719
NarI GG'CG_CC
Cuts at: 186 575 1150 1398 186
Size: 389 575 248 2507
Fragments arranged by size:
2507 575 389 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3719
NgoMIV G'CCGG_C
Cuts at: 1898 1898
Size: 3719
NotI GC'GGCC_GC
Cuts at: 234 234
Size: 3719
NspI r_CATG'y
Cuts at: 772 1272 2179 772
Size: 500 907 2312
Fragments arranged by size:
2312 907 500
PciI A'CATG_T
Cuts at: 2175 2175
Size: 3719
PfoI T'CCnGG_A
Cuts at: 356 356
Size: 3719
PmeI GTTT'AAAC
Cuts at: 718 718
Size: 3719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2858 2890 2858
Size: 32 3687
PpuMI rG'GwC_Cy
Cuts at: 413 441 413
Size: 28 3691
PspOMI G'GGCC_C
Cuts at: 552 628 552
Size: 76 3643
PstI C_TGCA'G
Cuts at: 269 320 766 1451 269
Size: 51 446 685 2537
Fragments arranged by size:
2537 685 446 51
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3719
PvuII CAG'CTG
Cuts at: 264 1144 1504 264
Size: 880 360 2479
Fragments arranged by size:
2479 880 360
RsrII CG'GwC_CG
Cuts at: 1914 1914
Size: 3719
SacI G_AGCT'C
Cuts at: 296 527 733 296
Size: 231 206 3282
Fragments arranged by size:
3282 231 206
SacII CC_GC'GG
Cuts at: 561 561
Size: 3719
SalI G'TCGA_C
Cuts at: 756 756
Size: 3719
SanDI GG'GwC_CC
Cuts at: 413 413
Size: 3719
SapI GCTCTTCn'nnn_
Cuts at: 167 412 1742 1952 167
Size: 245 1330 210 1934
Fragments arranged by size:
1934 1330 245 210
SbfI CC_TGCA'GG
Cuts at: 766 766
Size: 3719
ScaI AGT'ACT
Cuts at: 3236 3236
Size: 3719
SfcI C'TryA_G
Cuts at: 33 265 316 762 1447 2440 2631 33
Size: 232 51 446 685 993 191 1121
Fragments arranged by size:
1121 993 685 446 232 191 51
SfoI GGC'GCC
Cuts at: 187 576 1151 1399 187
Size: 389 575 248 2507
Fragments arranged by size:
2507 575 389 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3719
SmaI CCC'GGG
Cuts at: 427 741 427
Size: 314 3405
SmlI C'TyrA_G
Cuts at: 295 517 874 1789 2281 2543 295
Size: 222 357 915 492 262 1471
Fragments arranged by size:
1471 915 492 357 262 222
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3719
SphI G_CATG'C
Cuts at: 772 1272 772
Size: 500 3219
StyI C'CwwG_G
Cuts at: 107 639 853 1207 107
Size: 532 214 354 2619
Fragments arranged by size:
2619 532 354 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1302 1435 1894 2103 1302
Size: 133 459 209 2918
Fragments arranged by size:
2918 459 209 133
TatI w'GTAC_w
Cuts at: 3234 3234
Size: 3719
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 3687
Tth111I GACn'n_nGTC
Cuts at: 1516 1516
Size: 3719
XbaI T'CTAG_A
Cuts at: 750 750
Size: 3719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1214 1214
Size: 3719
XmaI C'CCGG_G
Cuts at: 425 739 425
Size: 314 3405
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PciI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AhdI AlfI AloI BaeI BbsI BbvCI
BcgI BfrBI BmgBI BmtI Bpu10I BsaXI BsiWI BsmI
BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI