Restriction map for
pF1KB9791
(Circular) (Six-base) MAPSORT of: pF1KB9791.seq Check: 9640 from: 1 to: 3743
pF1KB9791 3743 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 310 556 310
Size: 246 3497
AccI GT'mk_AC
Cuts at: 781 781
Size: 3743
Acc65I G'GTAC_C
Cuts at: 759 759
Size: 3743
AclI AA'CG_TT
Cuts at: 3583 3583
Size: 3743
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1568 2000 2747 3015 1568
Size: 432 747 268 2296
Fragments arranged by size:
2296 747 432 268
AflIII A'CryG_T
Cuts at: 1095 2199 3176 1095
Size: 1104 977 1662
Fragments arranged by size:
1662 1104 977
AgeI A'CCGG_T
Cuts at: 2131 2131
Size: 3743
AlwNI CAG_nnn'CTG
Cuts at: 413 1048 2615 413
Size: 635 1567 1541
Fragments arranged by size:
1567 1541 635
ApaI G_GGCC'C
Cuts at: 275 275
Size: 3743
ApaLI G'TGCA_C
Cuts at: 2513 2513
Size: 3743
ApoI r'AATT_y
Cuts at: 747 3096 3352 747
Size: 2349 256 1138
Fragments arranged by size:
2349 1138 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3743
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3743
AvaI C'yCGr_G
Cuts at: 239 558 763 239
Size: 319 205 3219
Fragments arranged by size:
3219 319 205
BamHI G'GATC_C
Cuts at: 768 768
Size: 3743
BanI G'GyrC_C
Cuts at: 259 322 477 759 1173 1421 1456 259
Size: 63 155 282 414 248 35 2546
Fragments arranged by size:
2546 414 282 248 155 63 35
BanII G_rGCy'C
Cuts at: 275 757 1787 275
Size: 482 1030 2231
Fragments arranged by size:
2231 1030 482
BbeI G_GCGC'C
Cuts at: 1177 1425 1177
Size: 248 3495
BbvCI CC'TCA_GC
Cuts at: 545 718 545
Size: 173 3570
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 463 497 90
Size: 34 339 34 3336
Fragments arranged by size:
3336 339 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1641 2402 3743 1641
Size: 761 1341 1641
Fragments arranged by size:
1641 1341 761
BclI T'GATC_A
Cuts at: 1263 1263
Size: 3743
BglI GCCn_nnn'nGGC
Cuts at: 671 728 671
Size: 57 3686
BglII A'GATC_T
Cuts at: 1258 3295 1258
Size: 2037 1706
BlpI GC'TnA_GC
Cuts at: 855 855
Size: 3743
Bme1580I G_kGCm'C
Cuts at: 264 275 327 1368 1461 2517 264
Size: 11 52 1041 93 1056 1490
Fragments arranged by size:
1490 1056 1041 93 52 11
BmrI ACTGGGnnnn_n'
Cuts at: 155 612 955 1036 1371 3515 155
Size: 457 343 81 335 2144 383
Fragments arranged by size:
2144 457 383 343 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2110 2110
Size: 3743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 313
Size: 3743
Bpu10I CC'TnA_GC
Cuts at: 545 718 545
Size: 173 3570
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 919 1798 2290 2588 919
Size: 879 492 298 2074
Fragments arranged by size:
2074 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3447 3447
Size: 3743
BsaAI yAC'GTr
Cuts at: 1096 1096
Size: 3743
BsaBI GATnn'nnATC
Cuts at: 6 1257 3294 6
Size: 1251 2037 455
Fragments arranged by size:
2037 1251 455
BsaHI Gr'CG_yC
Cuts at: 1174 1422 2124 3620 1174
Size: 248 702 1496 1297
Fragments arranged by size:
1496 1297 702 248
BsaWI w'CCGG_w
Cuts at: 932 1453 2131 2405 2552 2963 932
Size: 521 678 274 147 411 1712
Fragments arranged by size:
1712 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 217 247 217
Size: 30 3713
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 145 706 133
Size: 12 561 3170
Fragments arranged by size:
3170 561 12
BseYI C'CCAG_C
Cuts at: 268 298 413 1168 1926 2503 2999 268
Size: 30 115 755 758 577 496 1012
Fragments arranged by size:
1012 758 755 577 496 115 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 347 693 347
Size: 346 3397
BsiEI CG_ry'CG
Cuts at: 104 1285 1331 2539 104
Size: 1181 46 1208 1308
Fragments arranged by size:
1308 1208 1181 46
BsiHKAI G_wGCw'C
Cuts at: 757 1535 1725 2517 757
Size: 778 190 792 1983
Fragments arranged by size:
1983 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 264 275 327 757 1368 1461 1535 1725 1787
Size: 11 52 430 611 93 74 190 62
Cuts at: 1787 2517 264
Size: 730 1490
Fragments arranged by size:
1490 730 611 430 190 93 74 62
52 11
BspEI T'CCGG_A
Cuts at: 2963 2963
Size: 3743
BspHI T'CATG_A
Cuts at: 2919 2919
Size: 3743
BspMI ACCTGCnnnn'nnnn_
Cuts at: 310 556 793 1007 1309 1690 310
Size: 246 237 214 302 381 2363
Fragments arranged by size:
2363 381 302 246 237 214
BsrBI CCG'CTC
Cuts at: 50 2035 2089 3573 3738 50
Size: 1985 54 1484 165 55
Fragments arranged by size:
1985 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 196 1655 196
Size: 1459 2284
BsrFI r'CCGG_y
Cuts at: 341 1741 1922 2131 341
Size: 1400 181 209 1953
Fragments arranged by size:
1953 1400 209 181
BssSI C'ACGA_G
Cuts at: 2014 2372 2014
Size: 358 3385
BstAPI GCAn_nnn'nTGC
Cuts at: 1120 1120
Size: 3743
BstBI TT'CG_AA
Cuts at: 687 2104 687
Size: 1417 2326
BstYI r'GATC_y
Cuts at: 1 768 1235 1258 1593 1839 2851 2937 2949
Size: 767 467 23 335 246 1012 86 12
Cuts at: 2949 3295 1
Size: 346 449
Fragments arranged by size:
1012 767 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 413 693 1693 1874 2037 413
Size: 280 1000 181 163 2119
Fragments arranged by size:
2119 1000 280 181 163
BtsI GCAGTG_nn'
Cuts at: 695 986 695
Size: 291 3452
DraI TTT'AAA
Cuts at: 742 742
Size: 3743
DrdI GACnn_nn'nnGTC
Cuts at: 1449 2307 1449
Size: 858 2885
EaeI y'GGCC_r
Cuts at: 329 729 1328 1502 1893 1920 3265 329
Size: 400 599 174 391 27 1345 807
Fragments arranged by size:
1345 807 599 400 391 174 27
EagI C'GGCC_G
Cuts at: 1328 1328
Size: 3743
EarI CTCTTCn'nnn_
Cuts at: 289 1766 1976 289
Size: 1477 210 2056
Fragments arranged by size:
2056 1477 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 493 1951 2261 2407 3553 493
Size: 1458 310 146 1146 683
Fragments arranged by size:
1458 1146 683 310 146
EcoICRI GAG'CTC
Cuts at: 755 755
Size: 3743
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 1568 2000 2747 3015 313
Size: 1255 432 747 268 1041
Fragments arranged by size:
1255 1041 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 292 292
Size: 3743
EcoO109I rG'GnC_Cy
Cuts at: 225 272 302 882 225
Size: 47 30 580 3086
Fragments arranged by size:
3086 580 47 30
EcoRI G'AATT_C
Cuts at: 747 747
Size: 3743
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1762 1762
Size: 3743
FspI TGC'GCA
Cuts at: 1524 1524
Size: 3743
HaeII r_GCGC'y
Cuts at: 1177 1425 2447 3426 1177
Size: 248 1022 979 1494
Fragments arranged by size:
1494 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 217 249 1585 1615 1617 1647 217
Size: 32 1336 30 2 30 2313
Fragments arranged by size:
2313 1336 32 30 30 2
HincII GTy'rAC
Cuts at: 782 782
Size: 3743
KasI G'GCGC_C
Cuts at: 1173 1421 1173
Size: 248 3495
KpnI G_GTAC'C
Cuts at: 763 763
Size: 3743
MfeI C'AATT_G
Cuts at: 170 170
Size: 3743
MluI A'CGCG_T
Cuts at: 3176 3176
Size: 3743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2414 2598 2414
Size: 184 3559
MscI TGG'CCA
Cuts at: 1504 1504
Size: 3743
MslI CAynn'nnrTG
Cuts at: 1732 1859 1732
Size: 127 3616
MspA1I CmG'CkG
Cuts at: 436 487 527 652 854 1168 1528 2541 2786
Size: 51 40 125 202 314 360 1013 245
Cuts at: 2786 436
Size: 1393
Fragments arranged by size:
1393 1013 360 314 245 202 125 51
40
NaeI GCC'GGC
Cuts at: 343 1924 343
Size: 1581 2162
NarI GG'CG_CC
Cuts at: 1174 1422 1174
Size: 248 3495
NgoMIV G'CCGG_C
Cuts at: 341 1922 341
Size: 1581 2162
NspI r_CATG'y
Cuts at: 796 1296 2203 796
Size: 500 907 2336
Fragments arranged by size:
2336 907 500
PasI CC'CwG_GG
Cuts at: 660 660
Size: 3743
PciI A'CATG_T
Cuts at: 2199 2199
Size: 3743
PmeI GTTT'AAAC
Cuts at: 742 742
Size: 3743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2882 2914 2882
Size: 32 3711
PpuMI rG'GwC_Cy
Cuts at: 225 225
Size: 3743
PspOMI G'GGCC_C
Cuts at: 271 271
Size: 3743
PstI C_TGCA'G
Cuts at: 486 790 1475 486
Size: 304 685 2754
Fragments arranged by size:
2754 685 304
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3743
PvuII CAG'CTG
Cuts at: 487 1168 1528 487
Size: 681 360 2702
Fragments arranged by size:
2702 681 360
RsrII CG'GwC_CG
Cuts at: 431 1938 431
Size: 1507 2236
SacI G_AGCT'C
Cuts at: 757 757
Size: 3743
SalI G'TCGA_C
Cuts at: 780 780
Size: 3743
SapI GCTCTTCn'nnn_
Cuts at: 289 1766 1976 289
Size: 1477 210 2056
Fragments arranged by size:
2056 1477 210
SbfI CC_TGCA'GG
Cuts at: 790 790
Size: 3743
ScaI AGT'ACT
Cuts at: 511 3260 511
Size: 2749 994
SfcI C'TryA_G
Cuts at: 33 482 786 1471 2464 2655 33
Size: 449 304 685 993 191 1121
Fragments arranged by size:
1121 993 685 449 304 191
SfoI GGC'GCC
Cuts at: 1175 1423 1175
Size: 248 3495
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3743
SmaI CCC'GGG
Cuts at: 560 765 560
Size: 205 3538
SmlI C'TyrA_G
Cuts at: 898 1813 2305 2567 898
Size: 915 492 262 2074
Fragments arranged by size:
2074 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3743
SphI G_CATG'C
Cuts at: 796 1296 796
Size: 500 3243
StyI C'CwwG_G
Cuts at: 877 1231 877
Size: 354 3389
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 405 1326 1459 1918 2127 405
Size: 921 133 459 209 2021
Fragments arranged by size:
2021 921 459 209 133
TatI w'GTAC_w
Cuts at: 509 3258 509
Size: 2749 994
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3260 3292 3260
Size: 32 3711
Tth111I GACn'n_nGTC
Cuts at: 1540 1540
Size: 3743
XbaI T'CTAG_A
Cuts at: 774 774
Size: 3743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1238 1238
Size: 3743
XmaI C'CCGG_G
Cuts at: 558 763 558
Size: 205 3538
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI