Restriction map for
pF1KB9792
(Circular) (Six-base) MAPSORT of: pF1KB9792.seq Check: 8181 from: 1 to: 3743
pF1KB9792 3743 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 204 204
Size: 3743
AccI GT'mk_AC
Cuts at: 781 781
Size: 3743
Acc65I G'GTAC_C
Cuts at: 759 759
Size: 3743
AclI AA'CG_TT
Cuts at: 3583 3583
Size: 3743
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1568 2000 2747 3015 1568
Size: 432 747 268 2296
Fragments arranged by size:
2296 747 432 268
AfeI AGC'GCT
Cuts at: 346 466 346
Size: 120 3623
AflIII A'CryG_T
Cuts at: 383 1095 2199 3176 383
Size: 712 1104 977 950
Fragments arranged by size:
1104 977 950 712
AgeI A'CCGG_T
Cuts at: 2131 2131
Size: 3743
AlwNI CAG_nnn'CTG
Cuts at: 150 255 1048 2615 150
Size: 105 793 1567 1278
Fragments arranged by size:
1567 1278 793 105
ApaLI G'TGCA_C
Cuts at: 2513 2513
Size: 3743
ApoI r'AATT_y
Cuts at: 747 3096 3352 747
Size: 2349 256 1138
Fragments arranged by size:
2349 1138 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3743
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3743
AvaI C'yCGr_G
Cuts at: 417 763 417
Size: 346 3397
BamHI G'GATC_C
Cuts at: 768 768
Size: 3743
BanI G'GyrC_C
Cuts at: 412 759 1173 1421 1456 412
Size: 347 414 248 35 2699
Fragments arranged by size:
2699 414 347 248 35
BanII G_rGCy'C
Cuts at: 629 757 1787 629
Size: 128 1030 2585
Fragments arranged by size:
2585 1030 128
BbeI G_GCGC'C
Cuts at: 416 1177 1425 416
Size: 761 248 2734
Fragments arranged by size:
2734 761 248
BbsI GAAGACnn'nnnn_
Cuts at: 691 691
Size: 3743
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3709
BciVI GTATCCnnnnn_n'
Cuts at: 1641 2402 3743 1641
Size: 761 1341 1641
Fragments arranged by size:
1641 1341 761
BclI T'GATC_A
Cuts at: 1263 1263
Size: 3743
BglII A'GATC_T
Cuts at: 1258 3295 1258
Size: 2037 1706
BlpI GC'TnA_GC
Cuts at: 855 855
Size: 3743
Bme1580I G_kGCm'C
Cuts at: 424 595 1368 1461 2517 424
Size: 171 773 93 1056 1650
Fragments arranged by size:
1650 1056 773 171 93
BmrI ACTGGGnnnn_n'
Cuts at: 955 1036 1371 3515 955
Size: 81 335 2144 1183
Fragments arranged by size:
2144 1183 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2110 2110
Size: 3743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 724
Size: 3743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 919 1798 2290 2588 919
Size: 879 492 298 2074
Fragments arranged by size:
2074 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 558 3447 558
Size: 2889 854
BsaAI yAC'GTr
Cuts at: 228 533 1096 228
Size: 305 563 2875
Fragments arranged by size:
2875 563 305
BsaBI GATnn'nnATC
Cuts at: 6 169 1257 3294 6
Size: 163 1088 2037 455
Fragments arranged by size:
2037 1088 455 163
BsaHI Gr'CG_yC
Cuts at: 413 493 1174 1422 2124 3620 413
Size: 80 681 248 702 1496 536
Fragments arranged by size:
1496 702 681 536 248 80
BsaWI w'CCGG_w
Cuts at: 932 1453 2131 2405 2552 2963 932
Size: 521 678 274 147 411 1712
Fragments arranged by size:
1712 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 538 568 538
Size: 30 3713
BseYI C'CCAG_C
Cuts at: 150 255 1168 1926 2503 2999 150
Size: 105 913 758 577 496 894
Fragments arranged by size:
913 894 758 577 496 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 97 142 97
Size: 45 3698
BsiEI CG_ry'CG
Cuts at: 104 1285 1331 2539 104
Size: 1181 46 1208 1308
Fragments arranged by size:
1308 1208 1181 46
BsiHKAI G_wGCw'C
Cuts at: 757 1535 1725 2517 757
Size: 778 190 792 1983
Fragments arranged by size:
1983 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 424 595 629 757 1368 1461 1535 1725 1787
Size: 171 34 128 611 93 74 190 62
Cuts at: 1787 2517 424
Size: 730 1650
Fragments arranged by size:
1650 730 611 190 171 128 93 74
62 34
BspEI T'CCGG_A
Cuts at: 2963 2963
Size: 3743
BspHI T'CATG_A
Cuts at: 2919 2919
Size: 3743
BspMI ACCTGCnnnn'nnnn_
Cuts at: 204 793 1007 1309 1690 204
Size: 589 214 302 381 2257
Fragments arranged by size:
2257 589 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2035 2089 3573 3738 50
Size: 1985 54 1484 165 55
Fragments arranged by size:
1985 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1655 1655
Size: 3743
BsrFI r'CCGG_y
Cuts at: 1741 1922 2131 1741
Size: 181 209 3353
Fragments arranged by size:
3353 209 181
BssHII G'CGCG_C
Cuts at: 126 128 126
Size: 2 3741
BssSI C'ACGA_G
Cuts at: 615 2014 2372 615
Size: 1399 358 1986
Fragments arranged by size:
1986 1399 358
BstAPI GCAn_nnn'nTGC
Cuts at: 120 1120 120
Size: 1000 2743
BstBI TT'CG_AA
Cuts at: 2104 2104
Size: 3743
BstYI r'GATC_y
Cuts at: 1 164 768 1235 1258 1593 1839 2851 2937
Size: 163 604 467 23 335 246 1012 86
Cuts at: 2937 2949 3295 1
Size: 12 346 449
Fragments arranged by size:
1012 604 467 449 346 335 246 163
86 23 12
BtgI C'CryG_G
Cuts at: 689 689
Size: 3743
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1693 1874 2037 1693
Size: 181 163 3399
Fragments arranged by size:
3399 181 163
BtsI GCAGTG_nn'
Cuts at: 986 986
Size: 3743
DraI TTT'AAA
Cuts at: 742 742
Size: 3743
DraIII CAC_nnn'GTG
Cuts at: 120 120
Size: 3743
DrdI GACnn_nn'nnGTC
Cuts at: 1449 2307 1449
Size: 858 2885
EaeI y'GGCC_r
Cuts at: 1328 1502 1893 1920 3265 1328
Size: 174 391 27 1345 1806
Fragments arranged by size:
1806 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1328 1328
Size: 3743
EarI CTCTTCn'nnn_
Cuts at: 1766 1976 1766
Size: 210 3533
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 496 1951 2261 2407 3553 496
Size: 1455 310 146 1146 686
Fragments arranged by size:
1455 1146 686 310 146
EcoICRI GAG'CTC
Cuts at: 755 755
Size: 3743
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 1568 2000 2747 3015 724
Size: 844 432 747 268 1452
Fragments arranged by size:
1452 844 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 882 882
Size: 3743
EcoRI G'AATT_C
Cuts at: 747 747
Size: 3743
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1762 1762
Size: 3743
FspI TGC'GCA
Cuts at: 1524 1524
Size: 3743
HaeII r_GCGC'y
Cuts at: 348 416 468 1177 1425 2447 3426 348
Size: 68 52 709 248 1022 979 665
Fragments arranged by size:
1022 979 709 665 248 68 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 370 400 402 432 1585 1615 1617 1647 370
Size: 30 2 30 1153 30 2 30 2466
Fragments arranged by size:
2466 1153 30 30 30 30 2 2
HincII GTy'rAC
Cuts at: 782 782
Size: 3743
KasI G'GCGC_C
Cuts at: 412 1173 1421 412
Size: 761 248 2734
Fragments arranged by size:
2734 761 248
KpnI G_GTAC'C
Cuts at: 763 763
Size: 3743
MluI A'CGCG_T
Cuts at: 383 3176 383
Size: 2793 950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2414 2598 2414
Size: 184 3559
MscI TGG'CCA
Cuts at: 1504 1504
Size: 3743
MslI CAynn'nnrTG
Cuts at: 1732 1859 1732
Size: 127 3616
MspA1I CmG'CkG
Cuts at: 268 278 854 1168 1528 2541 2786 268
Size: 10 576 314 360 1013 245 1225
Fragments arranged by size:
1225 1013 576 360 314 245 10
NaeI GCC'GGC
Cuts at: 1924 1924
Size: 3743
NarI GG'CG_CC
Cuts at: 413 1174 1422 413
Size: 761 248 2734
Fragments arranged by size:
2734 761 248
NcoI C'CATG_G
Cuts at: 689 689
Size: 3743
NgoMIV G'CCGG_C
Cuts at: 1922 1922
Size: 3743
NspI r_CATG'y
Cuts at: 796 1296 2203 796
Size: 500 907 2336
Fragments arranged by size:
2336 907 500
PciI A'CATG_T
Cuts at: 2199 2199
Size: 3743
PflMI CCAn_nnn'nTGG
Cuts at: 150 186 602 150
Size: 36 416 3291
Fragments arranged by size:
3291 416 36
PmeI GTTT'AAAC
Cuts at: 742 742
Size: 3743
PmlI CAC'GTG
Cuts at: 228 228
Size: 3743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2882 2914 2882
Size: 32 3711
PstI C_TGCA'G
Cuts at: 790 1475 790
Size: 685 3058
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3743
PvuII CAG'CTG
Cuts at: 1168 1528 1168
Size: 360 3383
RsrII CG'GwC_CG
Cuts at: 1938 1938
Size: 3743
SacI G_AGCT'C
Cuts at: 757 757
Size: 3743
SalI G'TCGA_C
Cuts at: 780 780
Size: 3743
SapI GCTCTTCn'nnn_
Cuts at: 1766 1976 1766
Size: 210 3533
SbfI CC_TGCA'GG
Cuts at: 790 790
Size: 3743
ScaI AGT'ACT
Cuts at: 3260 3260
Size: 3743
SexAI A'CCwGG_T
Cuts at: 548 548
Size: 3743
SfcI C'TryA_G
Cuts at: 33 786 1471 2464 2655 33
Size: 753 685 993 191 1121
Fragments arranged by size:
1121 993 753 685 191
SfoI GGC'GCC
Cuts at: 414 1175 1423 414
Size: 761 248 2734
Fragments arranged by size:
2734 761 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3743
SmaI CCC'GGG
Cuts at: 765 765
Size: 3743
SmlI C'TyrA_G
Cuts at: 898 1813 2305 2567 898
Size: 915 492 262 2074
Fragments arranged by size:
2074 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3743
SphI G_CATG'C
Cuts at: 796 1296 796
Size: 500 3243
StyI C'CwwG_G
Cuts at: 428 470 476 641 689 877 1231 428
Size: 42 6 165 48 188 354 2940
Fragments arranged by size:
2940 354 188 165 48 42 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 441 1326 1459 1918 2127 441
Size: 885 133 459 209 2057
Fragments arranged by size:
2057 885 459 209 133
TatI w'GTAC_w
Cuts at: 3258 3258
Size: 3743
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3260 3292 3260
Size: 32 3711
Tth111I GACn'n_nGTC
Cuts at: 1540 1540
Size: 3743
XbaI T'CTAG_A
Cuts at: 774 774
Size: 3743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 556 1238 556
Size: 682 3061
XmaI C'CCGG_G
Cuts at: 763 763
Size: 3743
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PmlI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BseRI BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI