Restriction map for
pF1KB9793
(Circular) (Six-base) MAPSORT of: pF1KB9793.seq Check: 2614 from: 1 to: 3749
pF1KB9793 3749 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 787 787
Size: 3749
Acc65I G'GTAC_C
Cuts at: 765 765
Size: 3749
AclI AA'CG_TT
Cuts at: 3589 3589
Size: 3749
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1574 2006 2753 3021 1574
Size: 432 747 268 2302
Fragments arranged by size:
2302 747 432 268
AflIII A'CryG_T
Cuts at: 1101 2205 3182 1101
Size: 1104 977 1668
Fragments arranged by size:
1668 1104 977
AgeI A'CCGG_T
Cuts at: 2137 2137
Size: 3749
AleI CACnn'nnGTG
Cuts at: 262 262
Size: 3749
AlwNI CAG_nnn'CTG
Cuts at: 1054 2621 1054
Size: 1567 2182
ApaI G_GGCC'C
Cuts at: 549 549
Size: 3749
ApaLI G'TGCA_C
Cuts at: 2519 2519
Size: 3749
ApoI r'AATT_y
Cuts at: 753 3102 3358 753
Size: 2349 256 1144
Fragments arranged by size:
2349 1144 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3749
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
AvaI C'yCGr_G
Cuts at: 769 769
Size: 3749
BamHI G'GATC_C
Cuts at: 774 774
Size: 3749
BanI G'GyrC_C
Cuts at: 765 1179 1427 1462 765
Size: 414 248 35 3052
Fragments arranged by size:
3052 414 248 35
BanII G_rGCy'C
Cuts at: 200 549 625 763 1793 200
Size: 349 76 138 1030 2156
Fragments arranged by size:
2156 1030 349 138 76
BbeI G_GCGC'C
Cuts at: 1183 1431 1183
Size: 248 3501
BciVI GTATCCnnnnn_n'
Cuts at: 1647 2408 3749 1647
Size: 761 1341 1647
Fragments arranged by size:
1647 1341 761
BclI T'GATC_A
Cuts at: 1269 1269
Size: 3749
BglI GCCn_nnn'nGGC
Cuts at: 332 332
Size: 3749
BglII A'GATC_T
Cuts at: 1264 3301 1264
Size: 2037 1712
BlpI GC'TnA_GC
Cuts at: 861 861
Size: 3749
Bme1580I G_kGCm'C
Cuts at: 549 1374 1467 2523 549
Size: 825 93 1056 1775
Fragments arranged by size:
1775 1056 825 93
BmrI ACTGGGnnnn_n'
Cuts at: 961 1042 1377 3521 961
Size: 81 335 2144 1189
Fragments arranged by size:
2144 1189 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 577 2116 577
Size: 1539 2210
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 1804 2296 2594 925
Size: 879 492 298 2080
Fragments arranged by size:
2080 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 151 3453 151
Size: 3302 447
BsaAI yAC'GTr
Cuts at: 228 1102 228
Size: 874 2875
BsaBI GATnn'nnATC
Cuts at: 6 1263 3300 6
Size: 1257 2037 455
Fragments arranged by size:
2037 1257 455
BsaHI Gr'CG_yC
Cuts at: 1180 1428 2130 3626 1180
Size: 248 702 1496 1303
Fragments arranged by size:
1496 1303 702 248
BsaWI w'CCGG_w
Cuts at: 938 1459 2137 2411 2558 2969 938
Size: 521 678 274 147 411 1718
Fragments arranged by size:
1718 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 549 579 549
Size: 30 3719
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 181
Size: 3749
BseYI C'CCAG_C
Cuts at: 691 1174 1932 2509 3005 691
Size: 483 758 577 496 1435
Fragments arranged by size:
1435 758 577 496 483
BsiEI CG_ry'CG
Cuts at: 104 1291 1337 2545 104
Size: 1187 46 1208 1308
Fragments arranged by size:
1308 1208 1187 46
BsiHKAI G_wGCw'C
Cuts at: 625 763 1541 1731 2523 625
Size: 138 778 190 792 1851
Fragments arranged by size:
1851 792 778 190 138
BsmI GAATG_Cn'
Cuts at: 585 585
Size: 3749
Bsp1286I G_dGCh'C
Cuts at: 200 549 625 763 1374 1467 1541 1731 1793
Size: 349 76 138 611 93 74 190 62
Cuts at: 1793 2523 200
Size: 730 1426
Fragments arranged by size:
1426 730 611 349 190 138 93 76
74 62
BspEI T'CCGG_A
Cuts at: 2969 2969
Size: 3749
BspHI T'CATG_A
Cuts at: 2925 2925
Size: 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 479 799 1013 1315 1696 479
Size: 320 214 302 381 2532
Fragments arranged by size:
2532 381 320 302 214
BsrBI CCG'CTC
Cuts at: 50 2041 2095 3579 3744 50
Size: 1991 54 1484 165 55
Fragments arranged by size:
1991 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1661 1661
Size: 3749
BsrFI r'CCGG_y
Cuts at: 1747 1928 2137 1747
Size: 181 209 3359
Fragments arranged by size:
3359 209 181
BssSI C'ACGA_G
Cuts at: 2020 2378 2020
Size: 358 3391
BstAPI GCAn_nnn'nTGC
Cuts at: 1126 1126
Size: 3749
BstBI TT'CG_AA
Cuts at: 2110 2110
Size: 3749
BstYI r'GATC_y
Cuts at: 1 774 1241 1264 1599 1845 2857 2943 2955
Size: 773 467 23 335 246 1012 86 12
Cuts at: 2955 3301 1
Size: 346 449
Fragments arranged by size:
1012 773 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 131 161 278 398 539 665 131
Size: 30 117 120 141 126 3215
Fragments arranged by size:
3215 141 126 120 117 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1699 1880 2043 1699
Size: 181 163 3405
Fragments arranged by size:
3405 181 163
BtsI GCAGTG_nn'
Cuts at: 391 702 992 391
Size: 311 290 3148
Fragments arranged by size:
3148 311 290
DraI TTT'AAA
Cuts at: 748 748
Size: 3749
DrdI GACnn_nn'nnGTC
Cuts at: 1455 2313 1455
Size: 858 2891
EaeI y'GGCC_r
Cuts at: 378 1334 1508 1899 1926 3271 378
Size: 956 174 391 27 1345 856
Fragments arranged by size:
1345 956 856 391 174 27
EagI C'GGCC_G
Cuts at: 1334 1334
Size: 3749
EarI CTCTTCn'nnn_
Cuts at: 1772 1982 1772
Size: 210 3539
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1957 2267 2413 3559 1957
Size: 310 146 1146 2147
Fragments arranged by size:
2147 1146 310 146
EcoICRI GAG'CTC
Cuts at: 623 761 623
Size: 138 3611
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1574 2006 2753 3021 1574
Size: 432 747 268 2302
Fragments arranged by size:
2302 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 113 184 468 545 546 888 113
Size: 71 284 77 1 342 2974
Fragments arranged by size:
2974 342 284 77 71 1
EcoRI G'AATT_C
Cuts at: 753 753
Size: 3749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 659 1768 659
Size: 1109 2640
FspI TGC'GCA
Cuts at: 1530 1530
Size: 3749
HaeII r_GCGC'y
Cuts at: 1183 1431 2453 3432 1183
Size: 248 1022 979 1500
Fragments arranged by size:
1500 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1591 1621 1623 1653 1591
Size: 30 2 30 3687
Fragments arranged by size:
3687 30 30 2
HincII GTy'rAC
Cuts at: 788 788
Size: 3749
KasI G'GCGC_C
Cuts at: 1179 1427 1179
Size: 248 3501
KpnI G_GTAC'C
Cuts at: 769 769
Size: 3749
MluI A'CGCG_T
Cuts at: 3182 3182
Size: 3749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2420 2604 2420
Size: 184 3565
MscI TGG'CCA
Cuts at: 380 1510 380
Size: 1130 2619
MslI CAynn'nnrTG
Cuts at: 262 1738 1865 262
Size: 1476 127 2146
Fragments arranged by size:
2146 1476 127
MspA1I CmG'CkG
Cuts at: 860 1174 1534 2547 2792 860
Size: 314 360 1013 245 1817
Fragments arranged by size:
1817 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1930 1930
Size: 3749
NarI GG'CG_CC
Cuts at: 1180 1428 1180
Size: 248 3501
NcoI C'CATG_G
Cuts at: 398 539 665 398
Size: 141 126 3482
Fragments arranged by size:
3482 141 126
NgoMIV G'CCGG_C
Cuts at: 1928 1928
Size: 3749
NspI r_CATG'y
Cuts at: 802 1302 2209 802
Size: 500 907 2342
Fragments arranged by size:
2342 907 500
PasI CC'CwG_GG
Cuts at: 285 285
Size: 3749
PciI A'CATG_T
Cuts at: 2205 2205
Size: 3749
PflMI CCAn_nnn'nTGG
Cuts at: 666 666
Size: 3749
PfoI T'CCnGG_A
Cuts at: 186 186
Size: 3749
PmeI GTTT'AAAC
Cuts at: 748 748
Size: 3749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2888 2920 2888
Size: 32 3717
PpuMI rG'GwC_Cy
Cuts at: 113 184 468 113
Size: 71 284 3394
Fragments arranged by size:
3394 284 71
PspOMI G'GGCC_C
Cuts at: 545 545
Size: 3749
PstI C_TGCA'G
Cuts at: 796 1481 796
Size: 685 3064
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3749
PvuII CAG'CTG
Cuts at: 1174 1534 1174
Size: 360 3389
RsrII CG'GwC_CG
Cuts at: 1944 1944
Size: 3749
SacI G_AGCT'C
Cuts at: 625 763 625
Size: 138 3611
SalI G'TCGA_C
Cuts at: 786 786
Size: 3749
SapI GCTCTTCn'nnn_
Cuts at: 1772 1982 1772
Size: 210 3539
SbfI CC_TGCA'GG
Cuts at: 796 796
Size: 3749
ScaI AGT'ACT
Cuts at: 3266 3266
Size: 3749
SfcI C'TryA_G
Cuts at: 33 792 1477 2470 2661 33
Size: 759 685 993 191 1121
Fragments arranged by size:
1121 993 759 685 191
SfoI GGC'GCC
Cuts at: 1181 1429 1181
Size: 248 3501
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
SmaI CCC'GGG
Cuts at: 771 771
Size: 3749
SmlI C'TyrA_G
Cuts at: 904 1819 2311 2573 904
Size: 915 492 262 2080
Fragments arranged by size:
2080 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3749
SphI G_CATG'C
Cuts at: 802 1302 802
Size: 500 3249
StuI AGG'CCT
Cuts at: 322 322
Size: 3749
StyI C'CwwG_G
Cuts at: 398 475 539 665 883 1237 398
Size: 77 64 126 218 354 2910
Fragments arranged by size:
2910 354 218 126 77 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1332 1465 1924 2133 1332
Size: 133 459 209 2948
Fragments arranged by size:
2948 459 209 133
TatI w'GTAC_w
Cuts at: 3264 3264
Size: 3749
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 549 581 3266 3298 549
Size: 32 2685 32 1000
Fragments arranged by size:
2685 1000 32 32
Tth111I GACn'n_nGTC
Cuts at: 1546 1546
Size: 3749
XbaI T'CTAG_A
Cuts at: 780 780
Size: 3749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 264 396 1244 264
Size: 132 848 2769
Fragments arranged by size:
2769 848 132
XmaI C'CCGG_G
Cuts at: 769 769
Size: 3749
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BpmI Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI