Restriction map for
pF1KB9794
(Circular) (Six-base) MAPSORT of: pF1KB9794.seq Check: 3265 from: 1 to: 3764
pF1KB9794 3764 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 802 802
Size: 3764
Acc65I G'GTAC_C
Cuts at: 780 780
Size: 3764
AclI AA'CG_TT
Cuts at: 3604 3604
Size: 3764
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1589 2021 2768 3036 1589
Size: 432 747 268 2317
Fragments arranged by size:
2317 747 432 268
AfeI AGC'GCT
Cuts at: 137 137
Size: 3764
AflIII A'CryG_T
Cuts at: 1116 2220 3197 1116
Size: 1104 977 1683
Fragments arranged by size:
1683 1104 977
AgeI A'CCGG_T
Cuts at: 2152 2152
Size: 3764
AlwNI CAG_nnn'CTG
Cuts at: 1069 2636 1069
Size: 1567 2197
ApaLI G'TGCA_C
Cuts at: 2534 2534
Size: 3764
ApoI r'AATT_y
Cuts at: 768 3117 3373 768
Size: 2349 256 1159
Fragments arranged by size:
2349 1159 256
AscI GG'CGCG_CC
Cuts at: 263 263
Size: 3764
AseI AT'TA_AT
Cuts at: 20 20
Size: 3764
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
AvaI C'yCGr_G
Cuts at: 228 328 346 672 784 228
Size: 100 18 326 112 3208
Fragments arranged by size:
3208 326 112 100 18
BamHI G'GATC_C
Cuts at: 789 789
Size: 3764
BanI G'GyrC_C
Cuts at: 175 598 780 1194 1442 1477 175
Size: 423 182 414 248 35 2462
Fragments arranged by size:
2462 423 414 248 182 35
BanII G_rGCy'C
Cuts at: 152 778 1808 152
Size: 626 1030 2108
Fragments arranged by size:
2108 1030 626
BbeI G_GCGC'C
Cuts at: 179 1198 1446 179
Size: 1019 248 2497
Fragments arranged by size:
2497 1019 248
BbsI GAAGACnn'nnnn_
Cuts at: 728 728
Size: 3764
BbvCI CC'TCA_GC
Cuts at: 752 752
Size: 3764
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 624 658 624
Size: 34 3730
BciVI GTATCCnnnnn_n'
Cuts at: 1662 2423 3764 1662
Size: 761 1341 1662
Fragments arranged by size:
1662 1341 761
BclI T'GATC_A
Cuts at: 1284 1284
Size: 3764
BglI GCCn_nnn'nGGC
Cuts at: 147 147
Size: 3764
BglII A'GATC_T
Cuts at: 1279 3316 1279
Size: 2037 1727
BlpI GC'TnA_GC
Cuts at: 876 876
Size: 3764
Bme1580I G_kGCm'C
Cuts at: 601 647 1389 1482 2538 601
Size: 46 742 93 1056 1827
Fragments arranged by size:
1827 1056 742 93 46
BmgBI CAC'GTC
Cuts at: 444 444
Size: 3764
BmrI ACTGGGnnnn_n'
Cuts at: 976 1057 1392 3536 976
Size: 81 335 2144 1204
Fragments arranged by size:
2144 1204 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 3764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 541 204
Size: 337 3427
Bpu10I CC'TnA_GC
Cuts at: 752 752
Size: 3764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 940 1819 2311 2609 940
Size: 879 492 298 2095
Fragments arranged by size:
2095 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3468 3468
Size: 3764
BsaAI yAC'GTr
Cuts at: 1117 1117
Size: 3764
BsaBI GATnn'nnATC
Cuts at: 6 1278 3315 6
Size: 1272 2037 455
Fragments arranged by size:
2037 1272 455
BsaHI Gr'CG_yC
Cuts at: 176 1195 1443 2145 3641 176
Size: 1019 248 702 1496 299
Fragments arranged by size:
1496 1019 702 299 248
BsaWI w'CCGG_w
Cuts at: 953 1474 2152 2426 2573 2984 953
Size: 521 678 274 147 411 1733
Fragments arranged by size:
1733 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 580 580
Size: 3764
BseYI C'CCAG_C
Cuts at: 594 1189 1947 2524 3020 594
Size: 595 758 577 496 1338
Fragments arranged by size:
1338 758 595 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 613
Size: 3764
BsiEI CG_ry'CG
Cuts at: 104 516 1306 1352 2560 104
Size: 412 790 46 1208 1308
Fragments arranged by size:
1308 1208 790 412 46
BsiHKAI G_wGCw'C
Cuts at: 778 1556 1746 2538 778
Size: 778 190 792 2004
Fragments arranged by size:
2004 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 152 601 647 778 1389 1482 1556 1746 1808
Size: 449 46 131 611 93 74 190 62
Cuts at: 1808 2538 152
Size: 730 1378
Fragments arranged by size:
1378 730 611 449 190 131 93 74
62 46
BspEI T'CCGG_A
Cuts at: 2984 2984
Size: 3764
BspHI T'CATG_A
Cuts at: 2940 2940
Size: 3764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 287 389 561 814 1028 1330 1711 287
Size: 102 172 253 214 302 381 2340
Fragments arranged by size:
2340 381 302 253 214 172 102
BsrBI CCG'CTC
Cuts at: 50 188 2056 2110 3594 3759 50
Size: 138 1868 54 1484 165 55
Fragments arranged by size:
1868 1484 165 138 55 54
BsrDI GCAATG_nn'
Cuts at: 1676 1676
Size: 3764
BsrFI r'CCGG_y
Cuts at: 515 688 1762 1943 2152 515
Size: 173 1074 181 209 2127
Fragments arranged by size:
2127 1074 209 181 173
BssHII G'CGCG_C
Cuts at: 263 263
Size: 3764
BssSI C'ACGA_G
Cuts at: 2035 2393 2035
Size: 358 3406
BstAPI GCAn_nnn'nTGC
Cuts at: 1141 1141
Size: 3764
BstBI TT'CG_AA
Cuts at: 2125 2125
Size: 3764
BstEII G'GTnAC_C
Cuts at: 573 573
Size: 3764
BstYI r'GATC_y
Cuts at: 1 789 1256 1279 1614 1860 2872 2958 2970
Size: 788 467 23 335 246 1012 86 12
Cuts at: 2970 3316 1
Size: 346 449
Fragments arranged by size:
1012 788 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 235 107
Size: 128 3636
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1714 1895 2058 1714
Size: 181 163 3420
Fragments arranged by size:
3420 181 163
BtsI GCAGTG_nn'
Cuts at: 747 1007 747
Size: 260 3504
DraI TTT'AAA
Cuts at: 763 763
Size: 3764
DrdI GACnn_nn'nnGTC
Cuts at: 1470 2328 1470
Size: 858 2906
EaeI y'GGCC_r
Cuts at: 554 1349 1523 1914 1941 3286 554
Size: 795 174 391 27 1345 1032
Fragments arranged by size:
1345 1032 795 391 174 27
EagI C'GGCC_G
Cuts at: 1349 1349
Size: 3764
EarI CTCTTCn'nnn_
Cuts at: 152 1787 1997 152
Size: 1635 210 1919
Fragments arranged by size:
1919 1635 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 146 1972 2282 2428 3574 146
Size: 1826 310 146 1146 336
Fragments arranged by size:
1826 1146 336 310 146
EcoICRI GAG'CTC
Cuts at: 776 776
Size: 3764
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 541 1589 2021 2768 3036 204
Size: 337 1048 432 747 268 932
Fragments arranged by size:
1048 932 747 432 337 268
EcoNI CCTnn'n_nnAGG
Cuts at: 531 606 531
Size: 75 3689
EcoO109I rG'GnC_Cy
Cuts at: 357 378 903 357
Size: 21 525 3218
Fragments arranged by size:
3218 525 21
EcoRI G'AATT_C
Cuts at: 768 768
Size: 3764
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1783 1783
Size: 3764
FspI TGC'GCA
Cuts at: 1545 1545
Size: 3764
HaeII r_GCGC'y
Cuts at: 139 179 227 1198 1446 2468 3447 139
Size: 40 48 971 248 1022 979 456
Fragments arranged by size:
1022 979 971 456 248 48 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1606 1636 1638 1668 1606
Size: 30 2 30 3702
Fragments arranged by size:
3702 30 30 2
HincII GTy'rAC
Cuts at: 803 803
Size: 3764
KasI G'GCGC_C
Cuts at: 175 1194 1442 175
Size: 1019 248 2497
Fragments arranged by size:
2497 1019 248
KpnI G_GTAC'C
Cuts at: 784 784
Size: 3764
MluI A'CGCG_T
Cuts at: 3197 3197
Size: 3764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2435 2619 2435
Size: 184 3580
MscI TGG'CCA
Cuts at: 556 1525 556
Size: 969 2795
MslI CAynn'nnrTG
Cuts at: 1753 1880 1753
Size: 127 3637
MspA1I CmG'CkG
Cuts at: 285 301 875 1189 1549 2562 2807 285
Size: 16 574 314 360 1013 245 1242
Fragments arranged by size:
1242 1013 574 360 314 245 16
NaeI GCC'GGC
Cuts at: 690 1945 690
Size: 1255 2509
NarI GG'CG_CC
Cuts at: 176 1195 1443 176
Size: 1019 248 2497
Fragments arranged by size:
2497 1019 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3764
NgoMIV G'CCGG_C
Cuts at: 688 1943 688
Size: 1255 2509
NspI r_CATG'y
Cuts at: 414 423 817 1317 2224 414
Size: 9 394 500 907 1954
Fragments arranged by size:
1954 907 500 394 9
PasI CC'CwG_GG
Cuts at: 608 608
Size: 3764
PciI A'CATG_T
Cuts at: 2220 2220
Size: 3764
PmeI GTTT'AAAC
Cuts at: 763 763
Size: 3764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 631 663 2903 2935 631
Size: 32 2240 32 1460
Fragments arranged by size:
2240 1460 32 32
PpuMI rG'GwC_Cy
Cuts at: 378 378
Size: 3764
PspXI vC'TCGA_Gb
Cuts at: 672 672
Size: 3764
PstI C_TGCA'G
Cuts at: 811 1496 811
Size: 685 3079
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3764
PvuII CAG'CTG
Cuts at: 1189 1549 1189
Size: 360 3404
RsrII CG'GwC_CG
Cuts at: 716 1959 716
Size: 1243 2521
SacI G_AGCT'C
Cuts at: 778 778
Size: 3764
SalI G'TCGA_C
Cuts at: 801 801
Size: 3764
SapI GCTCTTCn'nnn_
Cuts at: 1787 1997 1787
Size: 210 3554
SbfI CC_TGCA'GG
Cuts at: 811 811
Size: 3764
ScaI AGT'ACT
Cuts at: 3281 3281
Size: 3764
SfcI C'TryA_G
Cuts at: 33 501 807 1492 2485 2676 33
Size: 468 306 685 993 191 1121
Fragments arranged by size:
1121 993 685 468 306 191
SfoI GGC'GCC
Cuts at: 177 1196 1444 177
Size: 1019 248 2497
Fragments arranged by size:
2497 1019 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
SmaI CCC'GGG
Cuts at: 330 786 330
Size: 456 3308
SmlI C'TyrA_G
Cuts at: 672 919 1834 2326 2588 672
Size: 247 915 492 262 1848
Fragments arranged by size:
1848 915 492 262 247
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3764
SphI G_CATG'C
Cuts at: 414 817 1317 414
Size: 403 500 2861
Fragments arranged by size:
2861 500 403
StuI AGG'CCT
Cuts at: 526 750 526
Size: 224 3540
StyI C'CwwG_G
Cuts at: 107 578 898 1252 107
Size: 471 320 354 2619
Fragments arranged by size:
2619 471 354 320
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 733 1347 1480 1939 2148 733
Size: 614 133 459 209 2349
Fragments arranged by size:
2349 614 459 209 133
TatI w'GTAC_w
Cuts at: 3279 3279
Size: 3764
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3281 3313 3281
Size: 32 3732
Tth111I GACn'n_nGTC
Cuts at: 1561 1561
Size: 3764
XbaI T'CTAG_A
Cuts at: 795 795
Size: 3764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1259 1259
Size: 3764
XhoI C'TCGA_G
Cuts at: 672 672
Size: 3764
XmaI C'CCGG_G
Cuts at: 328 784 328
Size: 456 3308
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PmeI PpiI PpuMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AvrII BaeI BfrBI BmtI BsaXI BsiWI BsmI BsmBI
BsrGI BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PshAI PsiI
PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XmnI ZraI