Restriction map for
pF1KB9804
(Circular) (Six-base) MAPSORT of: pF1KB9804.seq Check: 6137 from: 1 to: 3782
pF1KB9804 3782 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 820 820
Size: 3782
Acc65I G'GTAC_C
Cuts at: 798 798
Size: 3782
AclI AA'CG_TT
Cuts at: 3622 3622
Size: 3782
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1607 2039 2786 3054 1607
Size: 432 747 268 2335
Fragments arranged by size:
2335 747 432 268
AfeI AGC'GCT
Cuts at: 270 270
Size: 3782
AflIII A'CryG_T
Cuts at: 774 1134 2238 3215 774
Size: 360 1104 977 1341
Fragments arranged by size:
1341 1104 977 360
AgeI A'CCGG_T
Cuts at: 2170 2170
Size: 3782
AlwNI CAG_nnn'CTG
Cuts at: 1087 2654 1087
Size: 1567 2215
ApaI G_GGCC'C
Cuts at: 416 618 416
Size: 202 3580
ApaLI G'TGCA_C
Cuts at: 2552 2552
Size: 3782
ApoI r'AATT_y
Cuts at: 648 786 3135 3391 648
Size: 138 2349 256 1039
Fragments arranged by size:
2349 1039 256 138
AseI AT'TA_AT
Cuts at: 20 20
Size: 3782
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3782
AvaI C'yCGr_G
Cuts at: 619 802 619
Size: 183 3599
BamHI G'GATC_C
Cuts at: 807 807
Size: 3782
BanI G'GyrC_C
Cuts at: 524 682 798 1212 1460 1495 524
Size: 158 116 414 248 35 2811
Fragments arranged by size:
2811 414 248 158 116 35
BanII G_rGCy'C
Cuts at: 367 416 618 796 1826 367
Size: 49 202 178 1030 2323
Fragments arranged by size:
2323 1030 202 178 49
BbeI G_GCGC'C
Cuts at: 1216 1464 1216
Size: 248 3534
BbsI GAAGACnn'nnnn_
Cuts at: 409 409
Size: 3782
BbvCI CC'TCA_GC
Cuts at: 610 610
Size: 3782
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 244 278 90
Size: 34 120 34 3594
Fragments arranged by size:
3594 120 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1680 2441 3782 1680
Size: 761 1341 1680
Fragments arranged by size:
1680 1341 761
BclI T'GATC_A
Cuts at: 1302 1302
Size: 3782
BglI GCCn_nnn'nGGC
Cuts at: 478 487 478
Size: 9 3773
BglII A'GATC_T
Cuts at: 1297 3334 1297
Size: 2037 1745
BlpI GC'TnA_GC
Cuts at: 894 894
Size: 3782
Bme1580I G_kGCm'C
Cuts at: 416 618 1407 1500 2556 416
Size: 202 789 93 1056 1642
Fragments arranged by size:
1642 1056 789 202 93
BmrI ACTGGGnnnn_n'
Cuts at: 994 1075 1410 3554 994
Size: 81 335 2144 1222
Fragments arranged by size:
2144 1222 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 333 2149 333
Size: 1816 1966
Bpu10I CC'TnA_GC
Cuts at: 610 610
Size: 3782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 958 1837 2329 2627 958
Size: 879 492 298 2113
Fragments arranged by size:
2113 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 328 3486 328
Size: 3158 624
BsaAI yAC'GTr
Cuts at: 476 1135 476
Size: 659 3123
BsaBI GATnn'nnATC
Cuts at: 6 1296 3333 6
Size: 1290 2037 455
Fragments arranged by size:
2037 1290 455
BsaHI Gr'CG_yC
Cuts at: 1213 1461 2163 3659 1213
Size: 248 702 1496 1336
Fragments arranged by size:
1496 1336 702 248
BsaWI w'CCGG_w
Cuts at: 971 1492 2170 2444 2591 3002 971
Size: 521 678 274 147 411 1751
Fragments arranged by size:
1751 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 144 619 637 144
Size: 475 18 3289
Fragments arranged by size:
3289 475 18
BseYI C'CCAG_C
Cuts at: 659 1207 1965 2542 3038 659
Size: 548 758 577 496 1403
Fragments arranged by size:
1403 758 577 548 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 480 358
Size: 122 3660
BsiEI CG_ry'CG
Cuts at: 104 1324 1370 2578 104
Size: 1220 46 1208 1308
Fragments arranged by size:
1308 1220 1208 46
BsiHKAI G_wGCw'C
Cuts at: 796 1574 1764 2556 796
Size: 778 190 792 2022
Fragments arranged by size:
2022 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 367 416 618 796 1407 1500 1574 1764 1826
Size: 49 202 178 611 93 74 190 62
Cuts at: 1826 2556 367
Size: 730 1593
Fragments arranged by size:
1593 730 611 202 190 178 93 74
62 49
BspEI T'CCGG_A
Cuts at: 3002 3002
Size: 3782
BspHI T'CATG_A
Cuts at: 2958 2958
Size: 3782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 832 1046 1348 1729 832
Size: 214 302 381 2885
Fragments arranged by size:
2885 381 302 214
BsrBI CCG'CTC
Cuts at: 50 370 2074 2128 3612 3777 50
Size: 320 1704 54 1484 165 55
Fragments arranged by size:
1704 1484 320 165 55 54
BsrDI GCAATG_nn'
Cuts at: 285 1694 285
Size: 1409 2373
BsrFI r'CCGG_y
Cuts at: 1780 1961 2170 1780
Size: 181 209 3392
Fragments arranged by size:
3392 209 181
BssSI C'ACGA_G
Cuts at: 345 2053 2411 345
Size: 1708 358 1716
Fragments arranged by size:
1716 1708 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1159 1159
Size: 3782
BstBI TT'CG_AA
Cuts at: 2143 2143
Size: 3782
BstEII G'GTnAC_C
Cuts at: 574 574
Size: 3782
BstXI CCAn_nnnn'nTGG
Cuts at: 445 445
Size: 3782
BstYI r'GATC_y
Cuts at: 1 431 807 1274 1297 1632 1878 2890 2976
Size: 430 376 467 23 335 246 1012 86
Cuts at: 2976 2988 3334 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 430 376 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 320 729 320
Size: 409 3373
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1732 1913 2076 1732
Size: 181 163 3438
Fragments arranged by size:
3438 181 163
BtsI GCAGTG_nn'
Cuts at: 1025 1025
Size: 3782
DraI TTT'AAA
Cuts at: 781 781
Size: 3782
DrdI GACnn_nn'nnGTC
Cuts at: 1488 2346 1488
Size: 858 2924
EaeI y'GGCC_r
Cuts at: 708 732 1367 1541 1932 1959 3304 708
Size: 24 635 174 391 27 1345 1186
Fragments arranged by size:
1345 1186 635 391 174 27 24
EagI C'GGCC_G
Cuts at: 1367 1367
Size: 3782
EarI CTCTTCn'nnn_
Cuts at: 233 302 1805 2015 233
Size: 69 1503 210 2000
Fragments arranged by size:
2000 1503 210 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1990 2300 2446 3592 1990
Size: 310 146 1146 2180
Fragments arranged by size:
2180 1146 310 146
EcoICRI GAG'CTC
Cuts at: 794 794
Size: 3782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1607 2039 2786 3054 1607
Size: 432 747 268 2335
Fragments arranged by size:
2335 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 622 622
Size: 3782
EcoO109I rG'GnC_Cy
Cuts at: 413 614 615 921 413
Size: 201 1 306 3274
Fragments arranged by size:
3274 306 201 1
EcoRI G'AATT_C
Cuts at: 786 786
Size: 3782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1801 1801
Size: 3782
FspI TGC'GCA
Cuts at: 1563 1563
Size: 3782
HaeII r_GCGC'y
Cuts at: 272 1216 1464 2486 3465 272
Size: 944 248 1022 979 589
Fragments arranged by size:
1022 979 944 589 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 142 174 1624 1654 1656 1686 142
Size: 32 1450 30 2 30 2238
Fragments arranged by size:
2238 1450 32 30 30 2
HincII GTy'rAC
Cuts at: 821 821
Size: 3782
KasI G'GCGC_C
Cuts at: 1212 1460 1212
Size: 248 3534
KpnI G_GTAC'C
Cuts at: 802 802
Size: 3782
MluI A'CGCG_T
Cuts at: 3215 3215
Size: 3782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2453 2637 2453
Size: 184 3598
MscI TGG'CCA
Cuts at: 710 734 1543 710
Size: 24 809 2949
Fragments arranged by size:
2949 809 24
MslI CAynn'nnrTG
Cuts at: 1771 1898 1771
Size: 127 3655
MspA1I CmG'CkG
Cuts at: 484 549 663 893 1207 1567 2580 2825 484
Size: 65 114 230 314 360 1013 245 1441
Fragments arranged by size:
1441 1013 360 314 245 230 114 65
NaeI GCC'GGC
Cuts at: 1963 1963
Size: 3782
NarI GG'CG_CC
Cuts at: 1213 1461 1213
Size: 248 3534
NcoI C'CATG_G
Cuts at: 729 729
Size: 3782
NgoMIV G'CCGG_C
Cuts at: 1961 1961
Size: 3782
NspI r_CATG'y
Cuts at: 835 1335 2242 835
Size: 500 907 2375
Fragments arranged by size:
2375 907 500
PasI CC'CwG_GG
Cuts at: 516 516
Size: 3782
PciI A'CATG_T
Cuts at: 2238 2238
Size: 3782
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 3782
PmeI GTTT'AAAC
Cuts at: 781 781
Size: 3782
PmlI CAC'GTG
Cuts at: 476 476
Size: 3782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2921 2953 2921
Size: 32 3750
PspOMI G'GGCC_C
Cuts at: 412 614 412
Size: 202 3580
PspXI vC'TCGA_Gb
Cuts at: 619 619
Size: 3782
PstI C_TGCA'G
Cuts at: 548 829 1514 548
Size: 281 685 2816
Fragments arranged by size:
2816 685 281
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3782
PvuII CAG'CTG
Cuts at: 1207 1567 1207
Size: 360 3422
RsrII CG'GwC_CG
Cuts at: 1977 1977
Size: 3782
SacI G_AGCT'C
Cuts at: 796 796
Size: 3782
SalI G'TCGA_C
Cuts at: 819 819
Size: 3782
SapI GCTCTTCn'nnn_
Cuts at: 1805 2015 1805
Size: 210 3572
SbfI CC_TGCA'GG
Cuts at: 829 829
Size: 3782
ScaI AGT'ACT
Cuts at: 3299 3299
Size: 3782
SfcI C'TryA_G
Cuts at: 33 544 825 1510 2503 2694 33
Size: 511 281 685 993 191 1121
Fragments arranged by size:
1121 993 685 511 281 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 478 478
Size: 3782
SfoI GGC'GCC
Cuts at: 1214 1462 1214
Size: 248 3534
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3782
SmaI CCC'GGG
Cuts at: 804 804
Size: 3782
SmlI C'TyrA_G
Cuts at: 619 937 1852 2344 2606 619
Size: 318 915 492 262 1795
Fragments arranged by size:
1795 915 492 318 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3782
SphI G_CATG'C
Cuts at: 835 1335 835
Size: 500 3282
StyI C'CwwG_G
Cuts at: 500 587 729 916 1270 500
Size: 87 142 187 354 3012
Fragments arranged by size:
3012 354 187 142 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 529 673 752 1365 1498 1957 2166 529
Size: 144 79 613 133 459 209 2145
Fragments arranged by size:
2145 613 459 209 144 133 79
TatI w'GTAC_w
Cuts at: 3297 3297
Size: 3782
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 367 399 568 600 3299 3331 367
Size: 32 169 32 2699 32 818
Fragments arranged by size:
2699 818 169 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1579 1579
Size: 3782
XbaI T'CTAG_A
Cuts at: 813 813
Size: 3782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 295 442 727 1277 295
Size: 147 285 550 2800
Fragments arranged by size:
2800 550 285 147
XhoI C'TCGA_G
Cuts at: 619 619
Size: 3782
XmaI C'CCGG_G
Cuts at: 802 802
Size: 3782
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PmlI PpiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BfrBI BmgBI BmtI BpmI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PfoI PpuMI PshAI
PsiI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI
SspI StuI SwaI XmnI ZraI