Restriction map for
pF1KB9805
(Circular) (Six-base) MAPSORT of: pF1KB9805.seq Check: 1650 from: 1 to: 3788
pF1KB9805 3788 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 826 826
Size: 3788
Acc65I G'GTAC_C
Cuts at: 804 804
Size: 3788
AclI AA'CG_TT
Cuts at: 3628 3628
Size: 3788
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1613 2045 2792 3060 1613
Size: 432 747 268 2341
Fragments arranged by size:
2341 747 432 268
AflIII A'CryG_T
Cuts at: 137 1140 2244 3221 137
Size: 1003 1104 977 704
Fragments arranged by size:
1104 1003 977 704
AgeI A'CCGG_T
Cuts at: 2176 2176
Size: 3788
AlwNI CAG_nnn'CTG
Cuts at: 579 627 1093 2660 579
Size: 48 466 1567 1707
Fragments arranged by size:
1707 1567 466 48
ApaI G_GGCC'C
Cuts at: 241 713 241
Size: 472 3316
ApaLI G'TGCA_C
Cuts at: 2558 2558
Size: 3788
ApoI r'AATT_y
Cuts at: 792 3141 3397 792
Size: 2349 256 1183
Fragments arranged by size:
2349 1183 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3788
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
AvaI C'yCGr_G
Cuts at: 240 808 240
Size: 568 3220
BamHI G'GATC_C
Cuts at: 813 813
Size: 3788
BanI G'GyrC_C
Cuts at: 261 593 697 804 1218 1466 1501 261
Size: 332 104 107 414 248 35 2548
Fragments arranged by size:
2548 414 332 248 107 104 35
BanII G_rGCy'C
Cuts at: 119 241 713 802 1832 119
Size: 122 472 89 1030 2075
Fragments arranged by size:
2075 1030 472 122 89
BbeI G_GCGC'C
Cuts at: 265 597 701 1222 1470 265
Size: 332 104 521 248 2583
Fragments arranged by size:
2583 521 332 248 104
BciVI GTATCCnnnnn_n'
Cuts at: 1686 2447 3788 1686
Size: 761 1341 1686
Fragments arranged by size:
1686 1341 761
BclI T'GATC_A
Cuts at: 1308 1308
Size: 3788
BglI GCCn_nnn'nGGC
Cuts at: 193 245 193
Size: 52 3736
BglII A'GATC_T
Cuts at: 1303 3340 1303
Size: 2037 1751
BlpI GC'TnA_GC
Cuts at: 415 900 415
Size: 485 3303
Bme1580I G_kGCm'C
Cuts at: 241 685 713 1413 1506 2562 241
Size: 444 28 700 93 1056 1467
Fragments arranged by size:
1467 1056 700 444 93 28
BmrI ACTGGGnnnn_n'
Cuts at: 1000 1081 1416 3560 1000
Size: 81 335 2144 1228
Fragments arranged by size:
2144 1228 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 3788
Bpu10I CC'TnA_GC
Cuts at: 284 284
Size: 3788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 103 964 1843 2335 2633 103
Size: 861 879 492 298 1258
Fragments arranged by size:
1258 879 861 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 187 410 3492 187
Size: 223 3082 483
Fragments arranged by size:
3082 483 223
BsaAI yAC'GTr
Cuts at: 1141 1141
Size: 3788
BsaBI GATnn'nnATC
Cuts at: 6 1302 3339 6
Size: 1296 2037 455
Fragments arranged by size:
2037 1296 455
BsaHI Gr'CG_yC
Cuts at: 262 329 594 658 698 745 1219 1467 2169
Size: 67 265 64 40 47 474 248 702
Cuts at: 2169 3665 262
Size: 1496 385
Fragments arranged by size:
1496 702 474 385 265 248 67 64
47 40
BsaWI w'CCGG_w
Cuts at: 977 1498 2176 2450 2597 3008 977
Size: 521 678 274 147 411 1757
Fragments arranged by size:
1757 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 279 1213 1971 2548 3044 279
Size: 934 758 577 496 1023
Fragments arranged by size:
1023 934 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 251
Size: 3788
BsiEI CG_ry'CG
Cuts at: 104 391 1330 1376 2584 104
Size: 287 939 46 1208 1308
Fragments arranged by size:
1308 1208 939 287 46
BsiHKAI G_wGCw'C
Cuts at: 119 802 1580 1770 2562 119
Size: 683 778 190 792 1345
Fragments arranged by size:
1345 792 778 683 190
Bsp1286I G_dGCh'C
Cuts at: 119 241 685 713 802 1413 1506 1580 1770
Size: 122 444 28 89 611 93 74 190
Cuts at: 1770 1832 2562 119
Size: 62 730 1345
Fragments arranged by size:
1345 730 611 444 190 122 93 89
74 62 28
BspEI T'CCGG_A
Cuts at: 3008 3008
Size: 3788
BspHI T'CATG_A
Cuts at: 2964 2964
Size: 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 838 1052 1354 1735 838
Size: 214 302 381 2891
Fragments arranged by size:
2891 381 302 214
BsrBI CCG'CTC
Cuts at: 50 314 435 2080 2134 3618 3783 50
Size: 264 121 1645 54 1484 165 55
Fragments arranged by size:
1645 1484 264 165 121 55 54
BsrDI GCAATG_nn'
Cuts at: 1700 1700
Size: 3788
BsrFI r'CCGG_y
Cuts at: 780 1786 1967 2176 780
Size: 1006 181 209 2392
Fragments arranged by size:
2392 1006 209 181
BssHII G'CGCG_C
Cuts at: 427 427
Size: 3788
BssSI C'ACGA_G
Cuts at: 772 2059 2417 772
Size: 1287 358 2143
Fragments arranged by size:
2143 1287 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1165 1165
Size: 3788
BstBI TT'CG_AA
Cuts at: 2149 2149
Size: 3788
BstYI r'GATC_y
Cuts at: 1 813 1280 1303 1638 1884 2896 2982 2994
Size: 812 467 23 335 246 1012 86 12
Cuts at: 2994 3340 1
Size: 346 449
Fragments arranged by size:
1012 812 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 156 219 257 107
Size: 49 63 38 3638
Fragments arranged by size:
3638 63 49 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1738 1919 2082 1738
Size: 181 163 3444
Fragments arranged by size:
3444 181 163
BtsI GCAGTG_nn'
Cuts at: 1031 1031
Size: 3788
DraI TTT'AAA
Cuts at: 787 787
Size: 3788
DraIII CAC_nnn'GTG
Cuts at: 680 680
Size: 3788
DrdI GACnn_nn'nnGTC
Cuts at: 1494 2352 1494
Size: 858 2930
EaeI y'GGCC_r
Cuts at: 110 388 629 666 1373 1547 1938 1965 3310
Size: 278 241 37 707 174 391 27 1345
Cuts at: 3310 110
Size: 588
Fragments arranged by size:
1345 707 588 391 278 241 174 37
27
EagI C'GGCC_G
Cuts at: 388 1373 388
Size: 985 2803
EarI CTCTTCn'nnn_
Cuts at: 1811 2021 1811
Size: 210 3578
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 307 388 1996 2306 2452 3598 307
Size: 81 1608 310 146 1146 497
Fragments arranged by size:
1608 1146 497 310 146 81
EcoICRI GAG'CTC
Cuts at: 117 800 117
Size: 683 3105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1613 2045 2792 3060 1613
Size: 432 747 268 2341
Fragments arranged by size:
2341 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 565 613 565
Size: 48 3740
EcoO109I rG'GnC_Cy
Cuts at: 576 624 709 710 927 576
Size: 48 85 1 217 3437
Fragments arranged by size:
3437 217 85 48 1
EcoRI G'AATT_C
Cuts at: 792 792
Size: 3788
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1807 1807
Size: 3788
FspI TGC'GCA
Cuts at: 514 1569 514
Size: 1055 2733
HaeII r_GCGC'y
Cuts at: 265 597 701 1222 1470 2492 3471 265
Size: 332 104 521 248 1022 979 582
Fragments arranged by size:
1022 979 582 521 332 248 104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 106 138 388 420 470 502 1630 1660 1662
Size: 32 250 32 50 32 1128 30 2
Cuts at: 1662 1692 106
Size: 30 2202
Fragments arranged by size:
2202 1128 250 50 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 827 827
Size: 3788
KasI G'GCGC_C
Cuts at: 261 593 697 1218 1466 261
Size: 332 104 521 248 2583
Fragments arranged by size:
2583 521 332 248 104
KpnI G_GTAC'C
Cuts at: 808 808
Size: 3788
MluI A'CGCG_T
Cuts at: 137 3221 137
Size: 3084 704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2459 2643 2459
Size: 184 3604
MscI TGG'CCA
Cuts at: 1549 1549
Size: 3788
MslI CAynn'nnrTG
Cuts at: 1777 1904 1777
Size: 127 3661
MspA1I CmG'CkG
Cuts at: 221 259 302 342 402 899 1213 1573 2586
Size: 38 43 40 60 497 314 360 1013
Cuts at: 2586 2831 221
Size: 245 1178
Fragments arranged by size:
1178 1013 497 360 314 245 60 43
40 38
NaeI GCC'GGC
Cuts at: 1969 1969
Size: 3788
NarI GG'CG_CC
Cuts at: 262 594 698 1219 1467 262
Size: 332 104 521 248 2583
Fragments arranged by size:
2583 521 332 248 104
NcoI C'CATG_G
Cuts at: 107 107
Size: 3788
NgoMIV G'CCGG_C
Cuts at: 1967 1967
Size: 3788
NotI GC'GGCC_GC
Cuts at: 388 388
Size: 3788
NspI r_CATG'y
Cuts at: 447 841 1341 2248 447
Size: 394 500 907 1987
Fragments arranged by size:
1987 907 500 394
PasI CC'CwG_GG
Cuts at: 705 705
Size: 3788
PciI A'CATG_T
Cuts at: 2244 2244
Size: 3788
PflMI CCAn_nnn'nTGG
Cuts at: 627 627
Size: 3788
PfoI T'CCnGG_A
Cuts at: 739 739
Size: 3788
PmeI GTTT'AAAC
Cuts at: 787 787
Size: 3788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2927 2959 2927
Size: 32 3756
PspOMI G'GGCC_C
Cuts at: 237 709 237
Size: 472 3316
PstI C_TGCA'G
Cuts at: 835 1520 835
Size: 685 3103
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3788
PvuII CAG'CTG
Cuts at: 1213 1573 1213
Size: 360 3428
RsrII CG'GwC_CG
Cuts at: 1983 1983
Size: 3788
SacI G_AGCT'C
Cuts at: 119 802 119
Size: 683 3105
SacII CC_GC'GG
Cuts at: 222 260 222
Size: 38 3750
SalI G'TCGA_C
Cuts at: 825 825
Size: 3788
SapI GCTCTTCn'nnn_
Cuts at: 1811 2021 1811
Size: 210 3578
SbfI CC_TGCA'GG
Cuts at: 835 835
Size: 3788
ScaI AGT'ACT
Cuts at: 3305 3305
Size: 3788
SfcI C'TryA_G
Cuts at: 33 831 1516 2509 2700 33
Size: 798 685 993 191 1121
Fragments arranged by size:
1121 993 798 685 191
SfoI GGC'GCC
Cuts at: 263 595 699 1220 1468 263
Size: 332 104 521 248 2583
Fragments arranged by size:
2583 521 332 248 104
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
SmaI CCC'GGG
Cuts at: 242 810 242
Size: 568 3220
SmlI C'TyrA_G
Cuts at: 118 943 1858 2350 2612 118
Size: 825 915 492 262 1294
Fragments arranged by size:
1294 915 825 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3788
SphI G_CATG'C
Cuts at: 447 841 1341 447
Size: 394 500 2894
Fragments arranged by size:
2894 500 394
SrfI GCCC'GGGC
Cuts at: 242 242
Size: 3788
StyI C'CwwG_G
Cuts at: 107 922 1276 107
Size: 815 354 2619
Fragments arranged by size:
2619 815 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1371 1504 1963 2172 1371
Size: 133 459 209 2987
Fragments arranged by size:
2987 459 209 133
TatI w'GTAC_w
Cuts at: 3303 3303
Size: 3788
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3305 3337 3305
Size: 32 3756
Tth111I GACn'n_nGTC
Cuts at: 1585 1585
Size: 3788
XbaI T'CTAG_A
Cuts at: 819 819
Size: 3788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1283 1283
Size: 3788
XmaI C'CCGG_G
Cuts at: 240 808 240
Size: 568 3220
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI BpmI BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NruI NsiI
PacI PmlI PpuMI PshAI PsiI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SspI StuI SwaI XhoI
XmnI ZraI