Restriction map for
pF1KB9806
(Circular) (Six-base) MAPSORT of: pF1KB9806.seq Check: 4967 from: 1 to: 3791
pF1KB9806 3791 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 829 829
Size: 3791
Acc65I G'GTAC_C
Cuts at: 807 807
Size: 3791
AclI AA'CG_TT
Cuts at: 3631 3631
Size: 3791
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1616 2048 2795 3063 1616
Size: 432 747 268 2344
Fragments arranged by size:
2344 747 432 268
AflII C'TTAA_G
Cuts at: 301 301
Size: 3791
AflIII A'CryG_T
Cuts at: 1143 2247 3224 1143
Size: 1104 977 1710
Fragments arranged by size:
1710 1104 977
AgeI A'CCGG_T
Cuts at: 2179 2179
Size: 3791
AleI CACnn'nnGTG
Cuts at: 394 394
Size: 3791
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 412 412
Size: 3791
AlwNI CAG_nnn'CTG
Cuts at: 1096 2663 1096
Size: 1567 2224
ApaI G_GGCC'C
Cuts at: 230 230
Size: 3791
ApaLI G'TGCA_C
Cuts at: 2561 2561
Size: 3791
ApoI r'AATT_y
Cuts at: 795 3144 3400 795
Size: 2349 256 1186
Fragments arranged by size:
2349 1186 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3791
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
AvaI C'yCGr_G
Cuts at: 811 811
Size: 3791
BamHI G'GATC_C
Cuts at: 816 816
Size: 3791
BanI G'GyrC_C
Cuts at: 144 196 388 487 807 1221 1469 1504 144
Size: 52 192 99 320 414 248 35 2431
Fragments arranged by size:
2431 414 320 248 192 99 52 35
BanII G_rGCy'C
Cuts at: 230 628 805 1835 230
Size: 398 177 1030 2186
Fragments arranged by size:
2186 1030 398 177
BbeI G_GCGC'C
Cuts at: 148 491 1225 1473 148
Size: 343 734 248 2466
Fragments arranged by size:
2466 734 343 248
BbsI GAAGACnn'nnnn_
Cuts at: 574 574
Size: 3791
BciVI GTATCCnnnnn_n'
Cuts at: 1689 2450 3791 1689
Size: 761 1341 1689
Fragments arranged by size:
1689 1341 761
BclI T'GATC_A
Cuts at: 245 1311 245
Size: 1066 2725
BglI GCCn_nnn'nGGC
Cuts at: 142 316 481 538 757 766 142
Size: 174 165 57 219 9 3167
Fragments arranged by size:
3167 219 174 165 57 9
BglII A'GATC_T
Cuts at: 1306 3343 1306
Size: 2037 1754
BlpI GC'TnA_GC
Cuts at: 209 725 903 209
Size: 516 178 3097
Fragments arranged by size:
3097 516 178
Bme1580I G_kGCm'C
Cuts at: 230 391 412 1416 1509 2565 230
Size: 161 21 1004 93 1056 1456
Fragments arranged by size:
1456 1056 1004 161 93 21
BmgBI CAC'GTC
Cuts at: 333 333
Size: 3791
BmrI ACTGGGnnnn_n'
Cuts at: 226 1003 1084 1419 3563 226
Size: 777 81 335 2144 454
Fragments arranged by size:
2144 777 454 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 3791
Bpu10I CC'TnA_GC
Cuts at: 312 312
Size: 3791
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 967 1846 2338 2636 352
Size: 615 879 492 298 1507
Fragments arranged by size:
1507 879 615 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3495 3495
Size: 3791
BsaAI yAC'GTr
Cuts at: 1144 1144
Size: 3791
BsaBI GATnn'nnATC
Cuts at: 6 1305 3342 6
Size: 1299 2037 455
Fragments arranged by size:
2037 1299 455
BsaHI Gr'CG_yC
Cuts at: 145 488 605 1222 1470 2172 3668 145
Size: 343 117 617 248 702 1496 268
Fragments arranged by size:
1496 702 617 343 268 248 117
BsaWI w'CCGG_w
Cuts at: 980 1501 2179 2453 2600 3011 980
Size: 521 678 274 147 411 1760
Fragments arranged by size:
1760 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 360 1216 1974 2551 3047 360
Size: 856 758 577 496 1104
Fragments arranged by size:
1104 856 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 418
Size: 3791
BsiEI CG_ry'CG
Cuts at: 104 320 1333 1379 2587 104
Size: 216 1013 46 1208 1308
Fragments arranged by size:
1308 1208 1013 216 46
BsiHKAI G_wGCw'C
Cuts at: 805 1583 1773 2565 805
Size: 778 190 792 2031
Fragments arranged by size:
2031 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 230 391 412 628 805 1416 1509 1583 1773
Size: 161 21 216 177 611 93 74 190
Cuts at: 1773 1835 2565 230
Size: 62 730 1456
Fragments arranged by size:
1456 730 611 216 190 177 161 93
74 62 21
BspEI T'CCGG_A
Cuts at: 3011 3011
Size: 3791
BspHI T'CATG_A
Cuts at: 2967 2967
Size: 3791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 841 1055 1357 1738 841
Size: 214 302 381 2894
Fragments arranged by size:
2894 381 302 214
BsrBI CCG'CTC
Cuts at: 50 121 2083 2137 3621 3786 50
Size: 71 1962 54 1484 165 55
Fragments arranged by size:
1962 1484 165 71 55 54
BsrDI GCAATG_nn'
Cuts at: 285 1703 285
Size: 1418 2373
BsrFI r'CCGG_y
Cuts at: 147 193 199 319 1789 1970 2179 147
Size: 46 6 120 1470 181 209 1759
Fragments arranged by size:
1759 1470 209 181 120 46 6
BssSI C'ACGA_G
Cuts at: 2062 2420 2062
Size: 358 3433
BstAPI GCAn_nnn'nTGC
Cuts at: 1168 1168
Size: 3791
BstBI TT'CG_AA
Cuts at: 2152 2152
Size: 3791
BstXI CCAn_nnnn'nTGG
Cuts at: 258 258
Size: 3791
BstYI r'GATC_y
Cuts at: 1 816 1283 1306 1641 1887 2899 2985 2997
Size: 815 467 23 335 246 1012 86 12
Cuts at: 2997 3343 1
Size: 346 449
Fragments arranged by size:
1012 815 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 509 593 509
Size: 84 3707
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1741 1922 2085 1741
Size: 181 163 3447
Fragments arranged by size:
3447 181 163
BtsI GCAGTG_nn'
Cuts at: 566 1034 566
Size: 468 3323
DraI TTT'AAA
Cuts at: 432 790 432
Size: 358 3433
DrdI GACnn_nn'nnGTC
Cuts at: 1497 2355 1497
Size: 858 2933
EaeI y'GGCC_r
Cuts at: 317 1376 1550 1941 1968 3313 317
Size: 1059 174 391 27 1345 795
Fragments arranged by size:
1345 1059 795 391 174 27
EagI C'GGCC_G
Cuts at: 317 1376 317
Size: 1059 2732
EarI CTCTTCn'nnn_
Cuts at: 616 1814 2024 616
Size: 1198 210 2383
Fragments arranged by size:
2383 1198 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1999 2309 2455 3601 1999
Size: 310 146 1146 2189
Fragments arranged by size:
2189 1146 310 146
EcoICRI GAG'CTC
Cuts at: 803 803
Size: 3791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1616 2048 2795 3063 1616
Size: 432 747 268 2344
Fragments arranged by size:
2344 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 304 304
Size: 3791
EcoO109I rG'GnC_Cy
Cuts at: 226 227 930 226
Size: 1 703 3087
Fragments arranged by size:
3087 703 1
EcoRI G'AATT_C
Cuts at: 795 795
Size: 3791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 327 1810 327
Size: 1483 2308
FspI TGC'GCA
Cuts at: 1572 1572
Size: 3791
HaeII r_GCGC'y
Cuts at: 148 206 491 1225 1473 2495 3474 148
Size: 58 285 734 248 1022 979 465
Fragments arranged by size:
1022 979 734 465 285 248 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1633 1663 1665 1695 1633
Size: 30 2 30 3729
Fragments arranged by size:
3729 30 30 2
HincII GTy'rAC
Cuts at: 830 830
Size: 3791
KasI G'GCGC_C
Cuts at: 144 487 1221 1469 144
Size: 343 734 248 2466
Fragments arranged by size:
2466 734 343 248
KpnI G_GTAC'C
Cuts at: 811 811
Size: 3791
MluI A'CGCG_T
Cuts at: 3224 3224
Size: 3791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 92 2462 2646 92
Size: 2370 184 1237
Fragments arranged by size:
2370 1237 184
MscI TGG'CCA
Cuts at: 1552 1552
Size: 3791
MslI CAynn'nnrTG
Cuts at: 394 1780 1907 394
Size: 1386 127 2278
Fragments arranged by size:
2278 1386 127
MspA1I CmG'CkG
Cuts at: 511 595 646 902 1216 1576 2589 2834 511
Size: 84 51 256 314 360 1013 245 1468
Fragments arranged by size:
1468 1013 360 314 256 245 84 51
NaeI GCC'GGC
Cuts at: 201 1972 201
Size: 1771 2020
NarI GG'CG_CC
Cuts at: 145 488 1222 1470 145
Size: 343 734 248 2466
Fragments arranged by size:
2466 734 343 248
NgoMIV G'CCGG_C
Cuts at: 199 1970 199
Size: 1771 2020
NspI r_CATG'y
Cuts at: 844 1344 2251 844
Size: 500 907 2384
Fragments arranged by size:
2384 907 500
PciI A'CATG_T
Cuts at: 2247 2247
Size: 3791
PmeI GTTT'AAAC
Cuts at: 790 790
Size: 3791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2930 2962 2930
Size: 32 3759
PspOMI G'GGCC_C
Cuts at: 226 226
Size: 3791
PstI C_TGCA'G
Cuts at: 546 838 1523 546
Size: 292 685 2814
Fragments arranged by size:
2814 685 292
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3791
PvuII CAG'CTG
Cuts at: 1216 1576 1216
Size: 360 3431
RsrII CG'GwC_CG
Cuts at: 1986 1986
Size: 3791
SacI G_AGCT'C
Cuts at: 805 805
Size: 3791
SacII CC_GC'GG
Cuts at: 512 596 512
Size: 84 3707
SalI G'TCGA_C
Cuts at: 828 828
Size: 3791
SapI GCTCTTCn'nnn_
Cuts at: 616 1814 2024 616
Size: 1198 210 2383
Fragments arranged by size:
2383 1198 210
SbfI CC_TGCA'GG
Cuts at: 838 838
Size: 3791
ScaI AGT'ACT
Cuts at: 3308 3308
Size: 3791
SfcI C'TryA_G
Cuts at: 33 542 834 1519 2512 2703 33
Size: 509 292 685 993 191 1121
Fragments arranged by size:
1121 993 685 509 292 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 316 316
Size: 3791
SfoI GGC'GCC
Cuts at: 146 489 1223 1471 146
Size: 343 734 248 2466
Fragments arranged by size:
2466 734 343 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
SgrAI Cr'CCGG_yG
Cuts at: 147 147
Size: 3791
SmaI CCC'GGG
Cuts at: 813 813
Size: 3791
SmlI C'TyrA_G
Cuts at: 301 367 946 1861 2353 2615 301
Size: 66 579 915 492 262 1477
Fragments arranged by size:
1477 915 579 492 262 66
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3791
SphI G_CATG'C
Cuts at: 844 1344 844
Size: 500 3291
StuI AGG'CCT
Cuts at: 310 310
Size: 3791
StyI C'CwwG_G
Cuts at: 251 404 638 686 925 1279 251
Size: 153 234 48 239 354 2763
Fragments arranged by size:
2763 354 239 234 153 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1374 1507 1966 2175 1374
Size: 133 459 209 2990
Fragments arranged by size:
2990 459 209 133
TatI w'GTAC_w
Cuts at: 3306 3306
Size: 3791
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 3759
Tth111I GACn'n_nGTC
Cuts at: 1588 1588
Size: 3791
XbaI T'CTAG_A
Cuts at: 822 822
Size: 3791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1286 1286
Size: 3791
XmaI C'CCGG_G
Cuts at: 811 811
Size: 3791
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI BpmI BsaXI BseRI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SanDI SexAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI