Restriction map for
pF1KB9808
(Circular) (Six-base) MAPSORT of: pF1KB9808.seq Check: 3975 from: 1 to: 3836
pF1KB9808 3836 bp
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AccI GT'mk_AC
Cuts at: 874 874
Size: 3836
Acc65I G'GTAC_C
Cuts at: 852 852
Size: 3836
AclI AA'CG_TT
Cuts at: 3676 3676
Size: 3836
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 1661 2093 2840 3108 211
Size: 1450 432 747 268 939
Fragments arranged by size:
1450 939 747 432 268
AflIII A'CryG_T
Cuts at: 291 1188 2292 3269 291
Size: 897 1104 977 858
Fragments arranged by size:
1104 977 897 858
AgeI A'CCGG_T
Cuts at: 2224 2224
Size: 3836
AlwNI CAG_nnn'CTG
Cuts at: 414 1141 2708 414
Size: 727 1567 1542
Fragments arranged by size:
1567 1542 727
ApaLI G'TGCA_C
Cuts at: 2606 2606
Size: 3836
ApoI r'AATT_y
Cuts at: 502 840 3189 3445 502
Size: 338 2349 256 893
Fragments arranged by size:
2349 893 338 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3836
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
AvaI C'yCGr_G
Cuts at: 856 856
Size: 3836
BamHI G'GATC_C
Cuts at: 861 861
Size: 3836
BanI G'GyrC_C
Cuts at: 671 852 1266 1514 1549 671
Size: 181 414 248 35 2958
Fragments arranged by size:
2958 414 248 181 35
BanII G_rGCy'C
Cuts at: 261 850 1880 261
Size: 589 1030 2217
Fragments arranged by size:
2217 1030 589
BbeI G_GCGC'C
Cuts at: 1270 1518 1270
Size: 248 3588
BciVI GTATCCnnnnn_n'
Cuts at: 1734 2495 3836 1734
Size: 761 1341 1734
Fragments arranged by size:
1734 1341 761
BclI T'GATC_A
Cuts at: 131 1356 131
Size: 1225 2611
BglII A'GATC_T
Cuts at: 1351 3388 1351
Size: 2037 1799
BlpI GC'TnA_GC
Cuts at: 805 948 805
Size: 143 3693
Bme1580I G_kGCm'C
Cuts at: 1461 1554 2610 1461
Size: 93 1056 2687
Fragments arranged by size:
2687 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1048 1129 1464 3608 1048
Size: 81 335 2144 1276
Fragments arranged by size:
2144 1276 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 3836
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1012 1891 2383 2681 1012
Size: 879 492 298 2167
Fragments arranged by size:
2167 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 628 3540 628
Size: 2912 924
BsaAI yAC'GTr
Cuts at: 1189 1189
Size: 3836
BsaBI GATnn'nnATC
Cuts at: 6 1350 3387 6
Size: 1344 2037 455
Fragments arranged by size:
2037 1344 455
BsaHI Gr'CG_yC
Cuts at: 1267 1515 2217 3713 1267
Size: 248 702 1496 1390
Fragments arranged by size:
1496 1390 702 248
BsaWI w'CCGG_w
Cuts at: 776 1025 1546 2224 2498 2645 3056 776
Size: 249 521 678 274 147 411 1556
Fragments arranged by size:
1556 678 521 411 274 249 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 369 399 369
Size: 30 3806
BseYI C'CCAG_C
Cuts at: 380 1261 2019 2596 3092 380
Size: 881 758 577 496 1124
Fragments arranged by size:
1124 881 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 272 272
Size: 3836
BsiEI CG_ry'CG
Cuts at: 104 1378 1424 2632 104
Size: 1274 46 1208 1308
Fragments arranged by size:
1308 1274 1208 46
BsiHKAI G_wGCw'C
Cuts at: 806 850 1628 1818 2610 806
Size: 44 778 190 792 2032
Fragments arranged by size:
2032 792 778 190 44
BsmI GAATG_Cn'
Cuts at: 231 231
Size: 3836
Bsp1286I G_dGCh'C
Cuts at: 261 806 850 1461 1554 1628 1818 1880 2610
Size: 545 44 611 93 74 190 62 730
Cuts at: 2610 261
Size: 1487
Fragments arranged by size:
1487 730 611 545 190 93 74 62
44
BspEI T'CCGG_A
Cuts at: 776 3056 776
Size: 2280 1556
BspHI T'CATG_A
Cuts at: 3012 3012
Size: 3836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 886 1100 1402 1783 886
Size: 214 302 381 2939
Fragments arranged by size:
2939 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2128 2182 3666 3831 50
Size: 2078 54 1484 165 55
Fragments arranged by size:
2078 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 186 1748 186
Size: 1562 2274
BsrFI r'CCGG_y
Cuts at: 1834 2015 2224 1834
Size: 181 209 3446
Fragments arranged by size:
3446 209 181
BssSI C'ACGA_G
Cuts at: 2107 2465 2107
Size: 358 3478
BstAPI GCAn_nnn'nTGC
Cuts at: 1213 1213
Size: 3836
BstBI TT'CG_AA
Cuts at: 2197 2197
Size: 3836
BstYI r'GATC_y
Cuts at: 1 411 861 1328 1351 1686 1932 2944 3030
Size: 410 450 467 23 335 246 1012 86
Cuts at: 3030 3042 3388 1
Size: 12 346 449
Fragments arranged by size:
1012 467 450 449 410 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 743 743
Size: 3836
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1786 1967 2130 1786
Size: 181 163 3492
Fragments arranged by size:
3492 181 163
BtsI GCAGTG_nn'
Cuts at: 242 1079 242
Size: 837 2999
DraI TTT'AAA
Cuts at: 339 835 339
Size: 496 3340
DraIII CAC_nnn'GTG
Cuts at: 364 364
Size: 3836
DrdI GACnn_nn'nnGTC
Cuts at: 424 1542 2400 424
Size: 1118 858 1860
Fragments arranged by size:
1860 1118 858
EaeI y'GGCC_r
Cuts at: 1421 1595 1986 2013 3358 1421
Size: 174 391 27 1345 1899
Fragments arranged by size:
1899 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1421 1421
Size: 3836
EarI CTCTTCn'nnn_
Cuts at: 819 1859 2069 819
Size: 1040 210 2586
Fragments arranged by size:
2586 1040 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 175 2044 2354 2500 3646 175
Size: 1869 310 146 1146 365
Fragments arranged by size:
1869 1146 365 310 146
EcoICRI GAG'CTC
Cuts at: 848 848
Size: 3836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 1661 2093 2840 3108 211
Size: 1450 432 747 268 939
Fragments arranged by size:
1450 939 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 447 447
Size: 3836
EcoO109I rG'GnC_Cy
Cuts at: 648 975 648
Size: 327 3509
EcoRI G'AATT_C
Cuts at: 502 840 502
Size: 338 3498
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1855 1855
Size: 3836
FspI TGC'GCA
Cuts at: 1617 1617
Size: 3836
HaeII r_GCGC'y
Cuts at: 269 739 1270 1518 2540 3519 269
Size: 470 531 248 1022 979 586
Fragments arranged by size:
1022 979 586 531 470 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 185 217 1678 1708 1710 1740 185
Size: 32 1461 30 2 30 2281
Fragments arranged by size:
2281 1461 32 30 30 2
HincII GTy'rAC
Cuts at: 663 875 663
Size: 212 3624
KasI G'GCGC_C
Cuts at: 1266 1514 1266
Size: 248 3588
KpnI G_GTAC'C
Cuts at: 856 856
Size: 3836
MluI A'CGCG_T
Cuts at: 291 3269 291
Size: 2978 858
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2507 2691 2507
Size: 184 3652
MscI TGG'CCA
Cuts at: 1597 1597
Size: 3836
MslI CAynn'nnrTG
Cuts at: 112 319 1825 1952 112
Size: 207 1506 127 1996
Fragments arranged by size:
1996 1506 207 127
MspA1I CmG'CkG
Cuts at: 277 668 947 1261 1621 2634 2879 277
Size: 391 279 314 360 1013 245 1234
Fragments arranged by size:
1234 1013 391 360 314 279 245
NaeI GCC'GGC
Cuts at: 2017 2017
Size: 3836
NarI GG'CG_CC
Cuts at: 1267 1515 1267
Size: 248 3588
NcoI C'CATG_G
Cuts at: 743 743
Size: 3836
NgoMIV G'CCGG_C
Cuts at: 2015 2015
Size: 3836
NspI r_CATG'y
Cuts at: 889 1389 2296 889
Size: 500 907 2429
Fragments arranged by size:
2429 907 500
PciI A'CATG_T
Cuts at: 2292 2292
Size: 3836
PmeI GTTT'AAAC
Cuts at: 835 835
Size: 3836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2975 3007 2975
Size: 32 3804
PpuMI rG'GwC_Cy
Cuts at: 648 648
Size: 3836
PstI C_TGCA'G
Cuts at: 192 402 883 1568 192
Size: 210 481 685 2460
Fragments arranged by size:
2460 685 481 210
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3836
PvuII CAG'CTG
Cuts at: 1261 1621 1261
Size: 360 3476
RsrII CG'GwC_CG
Cuts at: 2031 2031
Size: 3836
SacI G_AGCT'C
Cuts at: 850 850
Size: 3836
SalI G'TCGA_C
Cuts at: 873 873
Size: 3836
SanDI GG'GwC_CC
Cuts at: 648 648
Size: 3836
SapI GCTCTTCn'nnn_
Cuts at: 819 1859 2069 819
Size: 1040 210 2586
Fragments arranged by size:
2586 1040 210
SbfI CC_TGCA'GG
Cuts at: 192 883 192
Size: 691 3145
ScaI AGT'ACT
Cuts at: 625 3353 625
Size: 2728 1108
SfcI C'TryA_G
Cuts at: 33 188 398 879 1564 2557 2748 33
Size: 155 210 481 685 993 191 1121
Fragments arranged by size:
1121 993 685 481 210 191 155
SfoI GGC'GCC
Cuts at: 1268 1516 1268
Size: 248 3588
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
SmaI CCC'GGG
Cuts at: 858 858
Size: 3836
SmlI C'TyrA_G
Cuts at: 991 1906 2398 2660 991
Size: 915 492 262 2167
Fragments arranged by size:
2167 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3836
SphI G_CATG'C
Cuts at: 889 1389 889
Size: 500 3336
StyI C'CwwG_G
Cuts at: 743 970 1324 743
Size: 227 354 3255
Fragments arranged by size:
3255 354 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 394 1419 1552 2011 2220 394
Size: 1025 133 459 209 2010
Fragments arranged by size:
2010 1025 459 209 133
TatI w'GTAC_w
Cuts at: 623 3351 623
Size: 2728 1108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 369 401 3353 3385 369
Size: 32 2952 32 820
Fragments arranged by size:
2952 820 32 32
Tth111I GACn'n_nGTC
Cuts at: 520 1633 520
Size: 1113 2723
XbaI T'CTAG_A
Cuts at: 867 867
Size: 3836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1331 1331
Size: 3836
XmaI C'CCGG_G
Cuts at: 856 856
Size: 3836
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BseRI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI