Restriction map for
pF1KB9810
(Circular) (Six-base) MAPSORT of: pF1KB9810.seq Check: 1052 from: 1 to: 3854
pF1KB9810 3854 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 892 892
Size: 3854
Acc65I G'GTAC_C
Cuts at: 870 870
Size: 3854
AclI AA'CG_TT
Cuts at: 3694 3694
Size: 3854
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 1679 2111 2858 3126 658
Size: 1021 432 747 268 1386
Fragments arranged by size:
1386 1021 747 432 268
AflIII A'CryG_T
Cuts at: 300 377 470 581 1206 2310 3287 300
Size: 77 93 111 625 1104 977 867
Fragments arranged by size:
1104 977 867 625 111 93 77
AgeI A'CCGG_T
Cuts at: 2242 2242
Size: 3854
AlwNI CAG_nnn'CTG
Cuts at: 1159 2726 1159
Size: 1567 2287
ApaI G_GGCC'C
Cuts at: 254 759 254
Size: 505 3349
ApaLI G'TGCA_C
Cuts at: 2624 2624
Size: 3854
ApoI r'AATT_y
Cuts at: 858 3207 3463 858
Size: 2349 256 1249
Fragments arranged by size:
2349 1249 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3854
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3854
AvaI C'yCGr_G
Cuts at: 874 874
Size: 3854
BamHI G'GATC_C
Cuts at: 879 879
Size: 3854
BanI G'GyrC_C
Cuts at: 488 870 1284 1532 1567 488
Size: 382 414 248 35 2775
Fragments arranged by size:
2775 414 382 248 35
BanII G_rGCy'C
Cuts at: 254 759 868 1898 254
Size: 505 109 1030 2210
Fragments arranged by size:
2210 1030 505 109
BbeI G_GCGC'C
Cuts at: 492 1288 1536 492
Size: 796 248 2810
Fragments arranged by size:
2810 796 248
BciVI GTATCCnnnnn_n'
Cuts at: 1752 2513 3854 1752
Size: 761 1341 1752
Fragments arranged by size:
1752 1341 761
BclI T'GATC_A
Cuts at: 1374 1374
Size: 3854
BglII A'GATC_T
Cuts at: 1369 3406 1369
Size: 2037 1817
BlpI GC'TnA_GC
Cuts at: 520 966 520
Size: 446 3408
Bme1580I G_kGCm'C
Cuts at: 254 759 1479 1572 2628 254
Size: 505 720 93 1056 1480
Fragments arranged by size:
1480 1056 720 505 93
BmgBI CAC'GTC
Cuts at: 380 380
Size: 3854
BmrI ACTGGGnnnn_n'
Cuts at: 720 735 1066 1147 1482 3626 720
Size: 15 331 81 335 2144 948
Fragments arranged by size:
2144 948 335 331 81 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2221 2221
Size: 3854
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 457 256
Size: 201 3653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1030 1909 2401 2699 1030
Size: 879 492 298 2185
Fragments arranged by size:
2185 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 649 3558 649
Size: 2909 945
BsaAI yAC'GTr
Cuts at: 303 750 1207 303
Size: 447 457 2950
Fragments arranged by size:
2950 457 447
BsaBI GATnn'nnATC
Cuts at: 6 1368 3405 6
Size: 1362 2037 455
Fragments arranged by size:
2037 1362 455
BsaHI Gr'CG_yC
Cuts at: 489 1285 1533 2235 3731 489
Size: 796 248 702 1496 612
Fragments arranged by size:
1496 796 702 612 248
BsaWI w'CCGG_w
Cuts at: 1043 1564 2242 2516 2663 3074 1043
Size: 521 678 274 147 411 1823
Fragments arranged by size:
1823 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 410 410
Size: 3854
BseYI C'CCAG_C
Cuts at: 1279 2037 2614 3110 1279
Size: 758 577 496 2023
Fragments arranged by size:
2023 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1396 1442 2650 104
Size: 1292 46 1208 1308
Fragments arranged by size:
1308 1292 1208 46
BsiHKAI G_wGCw'C
Cuts at: 868 1646 1836 2628 868
Size: 778 190 792 2094
Fragments arranged by size:
2094 792 778 190
BsiWI C'GTAC_G
Cuts at: 746 746
Size: 3854
Bsp1286I G_dGCh'C
Cuts at: 254 759 868 1479 1572 1646 1836 1898 2628
Size: 505 109 611 93 74 190 62 730
Cuts at: 2628 254
Size: 1480
Fragments arranged by size:
1480 730 611 505 190 109 93 74
62
BspEI T'CCGG_A
Cuts at: 3074 3074
Size: 3854
BspHI T'CATG_A
Cuts at: 3030 3030
Size: 3854
BspMI ACCTGCnnnn'nnnn_
Cuts at: 250 904 1118 1420 1801 250
Size: 654 214 302 381 2303
Fragments arranged by size:
2303 654 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2146 2200 3684 3849 50
Size: 2096 54 1484 165 55
Fragments arranged by size:
2096 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1766 1766
Size: 3854
BsrFI r'CCGG_y
Cuts at: 1852 2033 2242 1852
Size: 181 209 3464
Fragments arranged by size:
3464 209 181
BsrGI T'GTAC_A
Cuts at: 374 823 374
Size: 449 3405
BssSI C'ACGA_G
Cuts at: 2125 2483 2125
Size: 358 3496
BstAPI GCAn_nnn'nTGC
Cuts at: 1231 1231
Size: 3854
BstBI TT'CG_AA
Cuts at: 296 2215 296
Size: 1919 1935
BstYI r'GATC_y
Cuts at: 1 879 1346 1369 1704 1950 2962 3048 3060
Size: 878 467 23 335 246 1012 86 12
Cuts at: 3060 3406 1
Size: 346 449
Fragments arranged by size:
1012 878 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 225 671 764 225
Size: 446 93 3315
Fragments arranged by size:
3315 446 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1804 1985 2148 1804
Size: 181 163 3510
Fragments arranged by size:
3510 181 163
BtsI GCAGTG_nn'
Cuts at: 581 1097 581
Size: 516 3338
DraI TTT'AAA
Cuts at: 853 853
Size: 3854
DrdI GACnn_nn'nnGTC
Cuts at: 1560 2418 1560
Size: 858 2996
EaeI y'GGCC_r
Cuts at: 1439 1613 2004 2031 3376 1439
Size: 174 391 27 1345 1917
Fragments arranged by size:
1917 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1439 1439
Size: 3854
EarI CTCTTCn'nnn_
Cuts at: 305 428 840 1877 2087 305
Size: 123 412 1037 210 2072
Fragments arranged by size:
2072 1037 412 210 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2062 2372 2518 3664 2062
Size: 310 146 1146 2252
Fragments arranged by size:
2252 1146 310 146
EcoICRI GAG'CTC
Cuts at: 866 866
Size: 3854
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 457 658 1679 2111 2858 3126 256
Size: 201 201 1021 432 747 268 984
Fragments arranged by size:
1021 984 747 432 268 201 201
EcoNI CCTnn'n_nnAGG
Cuts at: 610 610
Size: 3854
EcoO109I rG'GnC_Cy
Cuts at: 615 756 993 615
Size: 141 237 3476
Fragments arranged by size:
3476 237 141
EcoRI G'AATT_C
Cuts at: 858 858
Size: 3854
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 663 1873 663
Size: 1210 2644
FspI TGC'GCA
Cuts at: 1635 1635
Size: 3854
HaeII r_GCGC'y
Cuts at: 492 1288 1536 2558 3537 492
Size: 796 248 1022 979 809
Fragments arranged by size:
1022 979 809 796 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1696 1726 1728 1758 1696
Size: 30 2 30 3792
Fragments arranged by size:
3792 30 30 2
HincII GTy'rAC
Cuts at: 893 893
Size: 3854
KasI G'GCGC_C
Cuts at: 488 1284 1532 488
Size: 796 248 2810
Fragments arranged by size:
2810 796 248
KpnI G_GTAC'C
Cuts at: 874 874
Size: 3854
MluI A'CGCG_T
Cuts at: 3287 3287
Size: 3854
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 299 2525 2709 299
Size: 2226 184 1444
Fragments arranged by size:
2226 1444 184
MscI TGG'CCA
Cuts at: 1615 1615
Size: 3854
MslI CAynn'nnrTG
Cuts at: 475 1843 1970 475
Size: 1368 127 2359
Fragments arranged by size:
2359 1368 127
MspA1I CmG'CkG
Cuts at: 965 1279 1639 2652 2897 965
Size: 314 360 1013 245 1922
Fragments arranged by size:
1922 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2035 2035
Size: 3854
NarI GG'CG_CC
Cuts at: 489 1285 1533 489
Size: 796 248 2810
Fragments arranged by size:
2810 796 248
NcoI C'CATG_G
Cuts at: 225 671 764 225
Size: 446 93 3315
Fragments arranged by size:
3315 446 93
NgoMIV G'CCGG_C
Cuts at: 2033 2033
Size: 3854
NspI r_CATG'y
Cuts at: 474 585 907 1407 2314 474
Size: 111 322 500 907 2014
Fragments arranged by size:
2014 907 500 322 111
PciI A'CATG_T
Cuts at: 470 581 2310 470
Size: 111 1729 2014
Fragments arranged by size:
2014 1729 111
PmeI GTTT'AAAC
Cuts at: 853 853
Size: 3854
PmlI CAC'GTG
Cuts at: 303 303
Size: 3854
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2993 3025 2993
Size: 32 3822
PpuMI rG'GwC_Cy
Cuts at: 615 615
Size: 3854
PspOMI G'GGCC_C
Cuts at: 250 755 250
Size: 505 3349
PstI C_TGCA'G
Cuts at: 576 697 901 1586 576
Size: 121 204 685 2844
Fragments arranged by size:
2844 685 204 121
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3854
PvuII CAG'CTG
Cuts at: 1279 1639 1279
Size: 360 3494
RsrII CG'GwC_CG
Cuts at: 2049 2049
Size: 3854
SacI G_AGCT'C
Cuts at: 868 868
Size: 3854
SalI G'TCGA_C
Cuts at: 891 891
Size: 3854
SapI GCTCTTCn'nnn_
Cuts at: 305 1877 2087 305
Size: 1572 210 2072
Fragments arranged by size:
2072 1572 210
SbfI CC_TGCA'GG
Cuts at: 901 901
Size: 3854
ScaI AGT'ACT
Cuts at: 3371 3371
Size: 3854
SfcI C'TryA_G
Cuts at: 33 572 693 897 1582 2575 2766 33
Size: 539 121 204 685 993 191 1121
Fragments arranged by size:
1121 993 685 539 204 191 121
SfoI GGC'GCC
Cuts at: 490 1286 1534 490
Size: 796 248 2810
Fragments arranged by size:
2810 796 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3854
SmaI CCC'GGG
Cuts at: 876 876
Size: 3854
SmlI C'TyrA_G
Cuts at: 1009 1924 2416 2678 1009
Size: 915 492 262 2185
Fragments arranged by size:
2185 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3854
SphI G_CATG'C
Cuts at: 907 1407 907
Size: 500 3354
StyI C'CwwG_G
Cuts at: 225 254 671 764 988 1342 225
Size: 29 417 93 224 354 2737
Fragments arranged by size:
2737 417 354 224 93 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1437 1570 2029 2238 1437
Size: 133 459 209 3053
Fragments arranged by size:
3053 459 209 133
TatI w'GTAC_w
Cuts at: 374 823 3369 374
Size: 449 2546 859
Fragments arranged by size:
2546 859 449
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3371 3403 3371
Size: 32 3822
Tth111I GACn'n_nGTC
Cuts at: 1651 1651
Size: 3854
XbaI T'CTAG_A
Cuts at: 885 885
Size: 3854
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1349 1349
Size: 3854
XmaI C'CCGG_G
Cuts at: 874 874
Size: 3854
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BsaXI BsgI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI