Restriction map for
pF1KB9812
(Circular) (Six-base) MAPSORT of: pF1KB9812.seq Check: 3801 from: 1 to: 3884
pF1KB9812 3884 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 922 922
Size: 3884
Acc65I G'GTAC_C
Cuts at: 900 900
Size: 3884
AclI AA'CG_TT
Cuts at: 3724 3724
Size: 3884
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 776 1709 2141 2888 3156 776
Size: 933 432 747 268 1504
Fragments arranged by size:
1504 933 747 432 268
AflIII A'CryG_T
Cuts at: 836 1236 2340 3317 836
Size: 400 1104 977 1403
Fragments arranged by size:
1403 1104 977 400
AgeI A'CCGG_T
Cuts at: 2272 2272
Size: 3884
AhdI GACnn_n'nnGTC
Cuts at: 622 622
Size: 3884
AlwNI CAG_nnn'CTG
Cuts at: 1189 2756 1189
Size: 1567 2317
ApaI G_GGCC'C
Cuts at: 338 338
Size: 3884
ApaLI G'TGCA_C
Cuts at: 2654 2654
Size: 3884
ApoI r'AATT_y
Cuts at: 888 3237 3493 888
Size: 2349 256 1279
Fragments arranged by size:
2349 1279 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3884
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3884
AvaI C'yCGr_G
Cuts at: 314 904 314
Size: 590 3294
BamHI G'GATC_C
Cuts at: 909 909
Size: 3884
BanI G'GyrC_C
Cuts at: 900 1314 1562 1597 900
Size: 414 248 35 3187
Fragments arranged by size:
3187 414 248 35
BanII G_rGCy'C
Cuts at: 338 449 898 1928 338
Size: 111 449 1030 2294
Fragments arranged by size:
2294 1030 449 111
BbeI G_GCGC'C
Cuts at: 1318 1566 1318
Size: 248 3636
BbsI GAAGACnn'nnnn_
Cuts at: 197 197
Size: 3884
BbvCI CC'TCA_GC
Cuts at: 305 305
Size: 3884
BciVI GTATCCnnnnn_n'
Cuts at: 818 1782 2543 3884 818
Size: 964 761 1341 818
Fragments arranged by size:
1341 964 818 761
BclI T'GATC_A
Cuts at: 1404 1404
Size: 3884
BglII A'GATC_T
Cuts at: 1399 3436 1399
Size: 2037 1847
BlpI GC'TnA_GC
Cuts at: 424 996 424
Size: 572 3312
Bme1580I G_kGCm'C
Cuts at: 338 1509 1602 2658 338
Size: 1171 93 1056 1564
Fragments arranged by size:
1564 1171 1056 93
BmgBI CAC'GTC
Cuts at: 839 839
Size: 3884
BmrI ACTGGGnnnn_n'
Cuts at: 1096 1177 1512 3656 1096
Size: 81 335 2144 1324
Fragments arranged by size:
2144 1324 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 3884
Bpu10I CC'TnA_GC
Cuts at: 305 305
Size: 3884
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1060 1939 2431 2729 1060
Size: 879 492 298 2215
Fragments arranged by size:
2215 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3588 3588
Size: 3884
BsaAI yAC'GTr
Cuts at: 477 1237 477
Size: 760 3124
BsaBI GATnn'nnATC
Cuts at: 6 1398 3435 6
Size: 1392 2037 455
Fragments arranged by size:
2037 1392 455
BsaHI Gr'CG_yC
Cuts at: 1315 1563 2265 3761 1315
Size: 248 702 1496 1438
Fragments arranged by size:
1496 1438 702 248
BsaWI w'CCGG_w
Cuts at: 1073 1594 2272 2546 2693 3104 1073
Size: 521 678 274 147 411 1853
Fragments arranged by size:
1853 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 235 265 235
Size: 30 3854
BseYI C'CCAG_C
Cuts at: 633 1309 2067 2644 3140 633
Size: 676 758 577 496 1377
Fragments arranged by size:
1377 758 676 577 496
BsiEI CG_ry'CG
Cuts at: 104 1426 1472 2680 104
Size: 1322 46 1208 1308
Fragments arranged by size:
1322 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 292 786 898 1676 1866 2658 292
Size: 494 112 778 190 792 1518
Fragments arranged by size:
1518 792 778 494 190 112
BsmI GAATG_Cn'
Cuts at: 349 349
Size: 3884
BsmBI CGTCTCn'nnnn_
Cuts at: 390 390
Size: 3884
Bsp1286I G_dGCh'C
Cuts at: 292 338 449 786 898 1509 1602 1676 1866
Size: 46 111 337 112 611 93 74 190
Cuts at: 1866 1928 2658 292
Size: 62 730 1518
Fragments arranged by size:
1518 730 611 337 190 112 111 93
74 62 46
BspEI T'CCGG_A
Cuts at: 3104 3104
Size: 3884
BspHI T'CATG_A
Cuts at: 3060 3060
Size: 3884
BspMI ACCTGCnnnn'nnnn_
Cuts at: 934 1148 1450 1831 934
Size: 214 302 381 2987
Fragments arranged by size:
2987 381 302 214
BsrBI CCG'CTC
Cuts at: 50 327 2176 2230 3714 3879 50
Size: 277 1849 54 1484 165 55
Fragments arranged by size:
1849 1484 277 165 55 54
BsrDI GCAATG_nn'
Cuts at: 374 1796 374
Size: 1422 2462
BsrFI r'CCGG_y
Cuts at: 705 1882 2063 2272 705
Size: 1177 181 209 2317
Fragments arranged by size:
2317 1177 209 181
BssSI C'ACGA_G
Cuts at: 808 2155 2513 808
Size: 1347 358 2179
Fragments arranged by size:
2179 1347 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1261 1261
Size: 3884
BstBI TT'CG_AA
Cuts at: 2245 2245
Size: 3884
BstEII G'GTnAC_C
Cuts at: 582 582
Size: 3884
BstXI CCAn_nnnn'nTGG
Cuts at: 261 261
Size: 3884
BstYI r'GATC_y
Cuts at: 1 909 1376 1399 1734 1980 2992 3078 3090
Size: 908 467 23 335 246 1012 86 12
Cuts at: 3090 3436 1
Size: 346 449
Fragments arranged by size:
1012 908 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 147 412 488 147
Size: 265 76 3543
Fragments arranged by size:
3543 265 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 702 1834 2015 2178 702
Size: 1132 181 163 2408
Fragments arranged by size:
2408 1132 181 163
BtsI GCAGTG_nn'
Cuts at: 1127 1127
Size: 3884
DraI TTT'AAA
Cuts at: 883 883
Size: 3884
DrdI GACnn_nn'nnGTC
Cuts at: 591 1590 2448 591
Size: 999 858 2027
Fragments arranged by size:
2027 999 858
EaeI y'GGCC_r
Cuts at: 599 1469 1643 2034 2061 3406 599
Size: 870 174 391 27 1345 1077
Fragments arranged by size:
1345 1077 870 391 174 27
EagI C'GGCC_G
Cuts at: 1469 1469
Size: 3884
EarI CTCTTCn'nnn_
Cuts at: 1907 2117 1907
Size: 210 3674
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 528 2092 2402 2548 3694 528
Size: 1564 310 146 1146 718
Fragments arranged by size:
1564 1146 718 310 146
EcoICRI GAG'CTC
Cuts at: 896 896
Size: 3884
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 776 1709 2141 2888 3156 776
Size: 933 432 747 268 1504
Fragments arranged by size:
1504 933 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 334 417 1023 334
Size: 83 606 3195
Fragments arranged by size:
3195 606 83
EcoRI G'AATT_C
Cuts at: 888 888
Size: 3884
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1903 1903
Size: 3884
FspI TGC'GCA
Cuts at: 1665 1665
Size: 3884
HaeII r_GCGC'y
Cuts at: 1318 1566 2588 3567 1318
Size: 248 1022 979 1635
Fragments arranged by size:
1635 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 580 608 612 640 1726 1756 1758 1788 580
Size: 28 4 28 1086 30 2 30 2676
Fragments arranged by size:
2676 1086 30 30 28 28 4 2
HincII GTy'rAC
Cuts at: 923 923
Size: 3884
KasI G'GCGC_C
Cuts at: 1314 1562 1314
Size: 248 3636
KpnI G_GTAC'C
Cuts at: 904 904
Size: 3884
MluI A'CGCG_T
Cuts at: 3317 3317
Size: 3884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2555 2739 2555
Size: 184 3700
MscI TGG'CCA
Cuts at: 1645 1645
Size: 3884
MslI CAynn'nnrTG
Cuts at: 1873 2000 1873
Size: 127 3757
MspA1I CmG'CkG
Cuts at: 414 995 1309 1669 2682 2927 414
Size: 581 314 360 1013 245 1371
Fragments arranged by size:
1371 1013 581 360 314 245
NaeI GCC'GGC
Cuts at: 2065 2065
Size: 3884
NarI GG'CG_CC
Cuts at: 1315 1563 1315
Size: 248 3636
NgoMIV G'CCGG_C
Cuts at: 2063 2063
Size: 3884
NspI r_CATG'y
Cuts at: 937 1437 2344 937
Size: 500 907 2477
Fragments arranged by size:
2477 907 500
PciI A'CATG_T
Cuts at: 2340 2340
Size: 3884
PmeI GTTT'AAAC
Cuts at: 883 883
Size: 3884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3023 3055 3023
Size: 32 3852
PpuMI rG'GwC_Cy
Cuts at: 417 417
Size: 3884
PspOMI G'GGCC_C
Cuts at: 334 334
Size: 3884
PstI C_TGCA'G
Cuts at: 931 1616 931
Size: 685 3199
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3884
PvuII CAG'CTG
Cuts at: 1309 1669 1309
Size: 360 3524
RsrII CG'GwC_CG
Cuts at: 2079 2079
Size: 3884
SacI G_AGCT'C
Cuts at: 898 898
Size: 3884
SacII CC_GC'GG
Cuts at: 415 415
Size: 3884
SalI G'TCGA_C
Cuts at: 921 921
Size: 3884
SapI GCTCTTCn'nnn_
Cuts at: 1907 2117 1907
Size: 210 3674
SbfI CC_TGCA'GG
Cuts at: 931 931
Size: 3884
ScaI AGT'ACT
Cuts at: 553 3401 553
Size: 2848 1036
SexAI A'CCwGG_T
Cuts at: 620 620
Size: 3884
SfcI C'TryA_G
Cuts at: 33 927 1612 2605 2796 33
Size: 894 685 993 191 1121
Fragments arranged by size:
1121 993 894 685 191
SfoI GGC'GCC
Cuts at: 1316 1564 1316
Size: 248 3636
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3884
SmaI CCC'GGG
Cuts at: 906 906
Size: 3884
SmlI C'TyrA_G
Cuts at: 1039 1954 2446 2708 1039
Size: 915 492 262 2215
Fragments arranged by size:
2215 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3884
SphI G_CATG'C
Cuts at: 937 1437 937
Size: 500 3384
SspI AAT'ATT
Cuts at: 673 673
Size: 3884
StyI C'CwwG_G
Cuts at: 156 440 1018 1372 156
Size: 284 578 354 2668
Fragments arranged by size:
2668 578 354 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 570 601 1467 1600 2059 2268 570
Size: 31 866 133 459 209 2186
Fragments arranged by size:
2186 866 459 209 133 31
TatI w'GTAC_w
Cuts at: 551 3399 551
Size: 2848 1036
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 401 433 3401 3433 401
Size: 32 2968 32 852
Fragments arranged by size:
2968 852 32 32
Tth111I GACn'n_nGTC
Cuts at: 1681 1681
Size: 3884
XbaI T'CTAG_A
Cuts at: 915 915
Size: 3884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 261 1379 261
Size: 1118 2766
XmaI C'CCGG_G
Cuts at: 904 904
Size: 3884
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BglI BmtI BpmI BseRI
BsgI BsiWI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspXI PsrI SanDI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI
ZraI