Restriction map for
pF1KB9814
(Circular) (Six-base) MAPSORT of: pF1KB9814.seq Check: 1224 from: 1 to: 3995
pF1KB9814 3995 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1033 1033
Size: 3995
Acc65I G'GTAC_C
Cuts at: 1011 1011
Size: 3995
AclI AA'CG_TT
Cuts at: 3835 3835
Size: 3995
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 524 1820 2252 2999 3267 524
Size: 1296 432 747 268 1252
Fragments arranged by size:
1296 1252 747 432 268
AflIII A'CryG_T
Cuts at: 1347 2451 3428 1347
Size: 1104 977 1914
Fragments arranged by size:
1914 1104 977
AgeI A'CCGG_T
Cuts at: 2383 2383
Size: 3995
AhdI GACnn_n'nnGTC
Cuts at: 517 517
Size: 3995
AlwNI CAG_nnn'CTG
Cuts at: 1300 2867 1300
Size: 1567 2428
ApaI G_GGCC'C
Cuts at: 622 622
Size: 3995
ApaLI G'TGCA_C
Cuts at: 2765 2765
Size: 3995
ApoI r'AATT_y
Cuts at: 219 999 3348 3604 219
Size: 780 2349 256 610
Fragments arranged by size:
2349 780 610 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3995
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3995
AvaI C'yCGr_G
Cuts at: 288 975 1015 288
Size: 687 40 3268
Fragments arranged by size:
3268 687 40
BamHI G'GATC_C
Cuts at: 1020 1020
Size: 3995
BanI G'GyrC_C
Cuts at: 478 1011 1425 1673 1708 478
Size: 533 414 248 35 2765
Fragments arranged by size:
2765 533 414 248 35
BanII G_rGCy'C
Cuts at: 341 537 600 622 803 902 1009 2039 341
Size: 196 63 22 181 99 107 1030 2297
Fragments arranged by size:
2297 1030 196 181 107 99 63 22
BbeI G_GCGC'C
Cuts at: 1429 1677 1429
Size: 248 3747
BciVI GTATCCnnnnn_n'
Cuts at: 1893 2654 3995 1893
Size: 761 1341 1893
Fragments arranged by size:
1893 1341 761
BclI T'GATC_A
Cuts at: 1515 1515
Size: 3995
BglI GCCn_nnn'nGGC
Cuts at: 149 691 149
Size: 542 3453
BglII A'GATC_T
Cuts at: 638 1510 3547 638
Size: 872 2037 1086
Fragments arranged by size:
2037 1086 872
BlpI GC'TnA_GC
Cuts at: 1107 1107
Size: 3995
Bme1580I G_kGCm'C
Cuts at: 246 622 1620 1713 2769 246
Size: 376 998 93 1056 1472
Fragments arranged by size:
1472 1056 998 376 93
BmrI ACTGGGnnnn_n'
Cuts at: 243 742 936 1207 1288 1623 3767 243
Size: 499 194 271 81 335 2144 471
Fragments arranged by size:
2144 499 471 335 271 194 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2362 2362
Size: 3995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 452 688 445
Size: 7 236 3752
Fragments arranged by size:
3752 236 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 392 1171 2050 2542 2840 364
Size: 28 779 879 492 298 1519
Fragments arranged by size:
1519 879 779 492 298 28
BsaI GGTCTCn'nnnn_
Cuts at: 480 865 965 3699 480
Size: 385 100 2734 776
Fragments arranged by size:
2734 776 385 100
BsaAI yAC'GTr
Cuts at: 1348 1348
Size: 3995
BsaBI GATnn'nnATC
Cuts at: 6 1509 3546 6
Size: 1503 2037 455
Fragments arranged by size:
2037 1503 455
BsaHI Gr'CG_yC
Cuts at: 1426 1674 2376 3872 1426
Size: 248 702 1496 1549
Fragments arranged by size:
1549 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1184 1705 2383 2657 2804 3215 1184
Size: 521 678 274 147 411 1964
Fragments arranged by size:
1964 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 201 349 973 201
Size: 148 624 3223
Fragments arranged by size:
3223 624 148
BseYI C'CCAG_C
Cuts at: 750 1420 2178 2755 3251 750
Size: 670 758 577 496 1494
Fragments arranged by size:
1494 758 670 577 496
BsiEI CG_ry'CG
Cuts at: 104 1537 1583 2791 104
Size: 1433 46 1208 1308
Fragments arranged by size:
1433 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 600 703 1009 1787 1977 2769 600
Size: 103 306 778 190 792 1826
Fragments arranged by size:
1826 792 778 306 190 103
Bsp1286I G_dGCh'C
Cuts at: 246 341 537 600 622 703 803 902 1009
Size: 95 196 63 22 81 100 99 107
Cuts at: 1009 1620 1713 1787 1977 2039 2769 246
Size: 611 93 74 190 62 730 1472
Fragments arranged by size:
1472 730 611 196 190 107 100 99
95 93 81 74 63 62 22
BspEI T'CCGG_A
Cuts at: 3215 3215
Size: 3995
BspHI T'CATG_A
Cuts at: 3171 3171
Size: 3995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1045 1259 1561 1942 1045
Size: 214 302 381 3098
Fragments arranged by size:
3098 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2287 2341 3825 3990 50
Size: 2237 54 1484 165 55
Fragments arranged by size:
2237 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1907 1907
Size: 3995
BsrFI r'CCGG_y
Cuts at: 1993 2174 2383 1993
Size: 181 209 3605
Fragments arranged by size:
3605 209 181
BssSI C'ACGA_G
Cuts at: 2266 2624 2266
Size: 358 3637
BstAPI GCAn_nnn'nTGC
Cuts at: 1372 1372
Size: 3995
BstBI TT'CG_AA
Cuts at: 2356 2356
Size: 3995
BstXI CCAn_nnnn'nTGG
Cuts at: 201 201
Size: 3995
BstYI r'GATC_y
Cuts at: 1 638 1020 1487 1510 1845 2091 3103 3189
Size: 637 382 467 23 335 246 1012 86
Cuts at: 3189 3201 3547 1
Size: 12 346 449
Fragments arranged by size:
1012 637 467 449 382 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 254 254
Size: 3995
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1945 2126 2289 1945
Size: 181 163 3651
Fragments arranged by size:
3651 181 163
BtsI GCAGTG_nn'
Cuts at: 709 1238 709
Size: 529 3466
DraI TTT'AAA
Cuts at: 994 994
Size: 3995
DrdI GACnn_nn'nnGTC
Cuts at: 1701 2559 1701
Size: 858 3137
EaeI y'GGCC_r
Cuts at: 917 1580 1754 2145 2172 3517 917
Size: 663 174 391 27 1345 1395
Fragments arranged by size:
1395 1345 663 391 174 27
EagI C'GGCC_G
Cuts at: 1580 1580
Size: 3995
EarI CTCTTCn'nnn_
Cuts at: 620 2018 2228 620
Size: 1398 210 2387
Fragments arranged by size:
2387 1398 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2203 2513 2659 3805 2203
Size: 310 146 1146 2393
Fragments arranged by size:
2393 1146 310 146
EcoICRI GAG'CTC
Cuts at: 598 1007 598
Size: 409 3586
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 452 524 688 1820 2252 2999 3267 445
Size: 7 72 164 1132 432 747 268 1173
Fragments arranged by size:
1173 1132 747 432 268 164 72 7
EcoNI CCTnn'n_nnAGG
Cuts at: 527 527
Size: 3995
EcoO109I rG'GnC_Cy
Cuts at: 618 1134 618
Size: 516 3479
EcoRI G'AATT_C
Cuts at: 219 999 219
Size: 780 3215
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2014 2014
Size: 3995
FspI TGC'GCA
Cuts at: 1776 1776
Size: 3995
HaeII r_GCGC'y
Cuts at: 1429 1677 2699 3678 1429
Size: 248 1022 979 1746
Fragments arranged by size:
1746 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 196 228 503 535 645 677 1837 1867 1869
Size: 32 275 32 110 32 1160 30 2
Cuts at: 1869 1899 196
Size: 30 2292
Fragments arranged by size:
2292 1160 275 110 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1034 1034
Size: 3995
KasI G'GCGC_C
Cuts at: 1425 1673 1425
Size: 248 3747
KpnI G_GTAC'C
Cuts at: 1015 1015
Size: 3995
MluI A'CGCG_T
Cuts at: 3428 3428
Size: 3995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 923 2666 2850 923
Size: 1743 184 2068
Fragments arranged by size:
2068 1743 184
MscI TGG'CCA
Cuts at: 1756 1756
Size: 3995
MslI CAynn'nnrTG
Cuts at: 1984 2111 1984
Size: 127 3868
MspA1I CmG'CkG
Cuts at: 750 1106 1420 1780 2793 3038 750
Size: 356 314 360 1013 245 1707
Fragments arranged by size:
1707 1013 360 356 314 245
NaeI GCC'GGC
Cuts at: 2176 2176
Size: 3995
NarI GG'CG_CC
Cuts at: 1426 1674 1426
Size: 248 3747
NcoI C'CATG_G
Cuts at: 254 254
Size: 3995
NgoMIV G'CCGG_C
Cuts at: 2174 2174
Size: 3995
NspI r_CATG'y
Cuts at: 1048 1548 2455 1048
Size: 500 907 2588
Fragments arranged by size:
2588 907 500
PasI CC'CwG_GG
Cuts at: 523 523
Size: 3995
PciI A'CATG_T
Cuts at: 2451 2451
Size: 3995
PflMI CCAn_nnn'nTGG
Cuts at: 926 926
Size: 3995
PfoI T'CCnGG_A
Cuts at: 893 893
Size: 3995
PmeI GTTT'AAAC
Cuts at: 994 994
Size: 3995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3134 3166 3134
Size: 32 3963
PspOMI G'GGCC_C
Cuts at: 618 618
Size: 3995
PstI C_TGCA'G
Cuts at: 638 869 1042 1727 638
Size: 231 173 685 2906
Fragments arranged by size:
2906 685 231 173
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3995
PvuII CAG'CTG
Cuts at: 1420 1780 1420
Size: 360 3635
RsrII CG'GwC_CG
Cuts at: 2190 2190
Size: 3995
SacI G_AGCT'C
Cuts at: 600 1009 600
Size: 409 3586
SalI G'TCGA_C
Cuts at: 1032 1032
Size: 3995
SapI GCTCTTCn'nnn_
Cuts at: 2018 2228 2018
Size: 210 3785
SbfI CC_TGCA'GG
Cuts at: 869 1042 869
Size: 173 3822
ScaI AGT'ACT
Cuts at: 3512 3512
Size: 3995
SexAI A'CCwGG_T
Cuts at: 549 549
Size: 3995
SfcI C'TryA_G
Cuts at: 33 634 865 1038 1723 2716 2907 33
Size: 601 231 173 685 993 191 1121
Fragments arranged by size:
1121 993 685 601 231 191 173
SfoI GGC'GCC
Cuts at: 1427 1675 1427
Size: 248 3747
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3995
SmaI CCC'GGG
Cuts at: 977 1017 977
Size: 40 3955
SmlI C'TyrA_G
Cuts at: 379 407 1150 2065 2557 2819 379
Size: 28 743 915 492 262 1555
Fragments arranged by size:
1555 915 743 492 262 28
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3995
SphI G_CATG'C
Cuts at: 1048 1548 1048
Size: 500 3495
StuI AGG'CCT
Cuts at: 171 871 171
Size: 700 3295
StyI C'CwwG_G
Cuts at: 145 254 722 872 1129 1483 145
Size: 109 468 150 257 354 2657
Fragments arranged by size:
2657 468 354 257 150 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1578 1711 2170 2379 1578
Size: 133 459 209 3194
Fragments arranged by size:
3194 459 209 133
TatI w'GTAC_w
Cuts at: 3510 3510
Size: 3995
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3512 3544 3512
Size: 32 3963
Tth111I GACn'n_nGTC
Cuts at: 1792 1792
Size: 3995
XbaI T'CTAG_A
Cuts at: 1026 1026
Size: 3995
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 261 1490 261
Size: 1229 2766
XmaI C'CCGG_G
Cuts at: 975 1015 975
Size: 40 3955
XmnI GAAnn'nnTTC
Cuts at: 372 372
Size: 3995
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI