Restriction map for
pF1KB9815
(Circular) (Six-base) MAPSORT of: pF1KB9815.seq Check: 8579 from: 1 to: 4010
pF1KB9815 4010 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 705 705
Size: 4010
AccI GT'mk_AC
Cuts at: 1048 1048
Size: 4010
Acc65I G'GTAC_C
Cuts at: 1026 1026
Size: 4010
AclI AA'CG_TT
Cuts at: 3850 3850
Size: 4010
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1835 2267 3014 3282 1835
Size: 432 747 268 2563
Fragments arranged by size:
2563 747 432 268
AflIII A'CryG_T
Cuts at: 940 1362 2466 3443 940
Size: 422 1104 977 1507
Fragments arranged by size:
1507 1104 977 422
AgeI A'CCGG_T
Cuts at: 2398 2398
Size: 4010
AhdI GACnn_n'nnGTC
Cuts at: 480 480
Size: 4010
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 538 570 538
Size: 32 3978
AlwNI CAG_nnn'CTG
Cuts at: 549 1315 2882 549
Size: 766 1567 1677
Fragments arranged by size:
1677 1567 766
ApaI G_GGCC'C
Cuts at: 785 785
Size: 4010
ApaLI G'TGCA_C
Cuts at: 865 2780 865
Size: 1915 2095
ApoI r'AATT_y
Cuts at: 1014 3363 3619 1014
Size: 2349 256 1405
Fragments arranged by size:
2349 1405 256
AscI GG'CGCG_CC
Cuts at: 368 368
Size: 4010
AseI AT'TA_AT
Cuts at: 20 20
Size: 4010
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
AvaI C'yCGr_G
Cuts at: 120 246 343 738 793 1030 120
Size: 126 97 395 55 237 3100
Fragments arranged by size:
3100 395 237 126 97 55
BamHI G'GATC_C
Cuts at: 151 992 1035 151
Size: 841 43 3126
Fragments arranged by size:
3126 841 43
BanI G'GyrC_C
Cuts at: 573 895 1026 1440 1688 1723 573
Size: 322 131 414 248 35 2860
Fragments arranged by size:
2860 414 322 248 131 35
BanII G_rGCy'C
Cuts at: 127 247 785 854 1024 2054 127
Size: 120 538 69 170 1030 2083
Fragments arranged by size:
2083 1030 538 170 120 69
BbeI G_GCGC'C
Cuts at: 1444 1692 1444
Size: 248 3762
BbvCI CC'TCA_GC
Cuts at: 178 178
Size: 4010
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 503 537 949 983 503
Size: 34 412 34 3530
Fragments arranged by size:
3530 412 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1908 2669 4010 1908
Size: 761 1341 1908
Fragments arranged by size:
1908 1341 761
BclI T'GATC_A
Cuts at: 1530 1530
Size: 4010
BglI GCCn_nnn'nGGC
Cuts at: 797 797
Size: 4010
BglII A'GATC_T
Cuts at: 1525 3562 1525
Size: 2037 1973
BlpI GC'TnA_GC
Cuts at: 1122 1122
Size: 4010
Bme1580I G_kGCm'C
Cuts at: 578 785 802 869 1635 1728 2784 578
Size: 207 17 67 766 93 1056 1804
Fragments arranged by size:
1804 1056 766 207 93 67 17
BmrI ACTGGGnnnn_n'
Cuts at: 915 1222 1303 1638 3782 915
Size: 307 81 335 2144 1143
Fragments arranged by size:
2144 1143 335 307 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 873 2377 873
Size: 1504 2506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 571 664 868 235
Size: 336 93 204 3377
Fragments arranged by size:
3377 336 204 93
Bpu10I CC'TnA_GC
Cuts at: 178 178
Size: 4010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1186 2065 2557 2855 1186
Size: 879 492 298 2341
Fragments arranged by size:
2341 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3714 3714
Size: 4010
BsaAI yAC'GTr
Cuts at: 498 1363 498
Size: 865 3145
BsaBI GATnn'nnATC
Cuts at: 6 1524 3561 6
Size: 1518 2037 455
Fragments arranged by size:
2037 1518 455
BsaHI Gr'CG_yC
Cuts at: 428 1441 1689 2391 3887 428
Size: 1013 248 702 1496 551
Fragments arranged by size:
1496 1013 702 551 248
BsaWI w'CCGG_w
Cuts at: 1199 1720 2398 2672 2819 3230 1199
Size: 521 678 274 147 411 1979
Fragments arranged by size:
1979 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 334 1435 2193 2770 3266 334
Size: 1101 758 577 496 1078
Fragments arranged by size:
1101 1078 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 943 478
Size: 465 3545
BsiEI CG_ry'CG
Cuts at: 104 391 1552 1598 2806 104
Size: 287 1161 46 1208 1308
Fragments arranged by size:
1308 1208 1161 287 46
BsiHKAI G_wGCw'C
Cuts at: 127 854 869 1024 1802 1992 2784 127
Size: 727 15 155 778 190 792 1353
Fragments arranged by size:
1353 792 778 727 190 155 15
BsmBI CGTCTCn'nnnn_
Cuts at: 467 467
Size: 4010
Bsp1286I G_dGCh'C
Cuts at: 127 247 578 785 802 854 869 1024 1635
Size: 120 331 207 17 52 15 155 611
Cuts at: 1635 1728 1802 1992 2054 2784 127
Size: 93 74 190 62 730 1353
Fragments arranged by size:
1353 730 611 331 207 190 155 120
93 74 62 52 17 15
BspEI T'CCGG_A
Cuts at: 3230 3230
Size: 4010
BspHI T'CATG_A
Cuts at: 3186 3186
Size: 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 705 1060 1274 1576 1957 705
Size: 355 214 302 381 2758
Fragments arranged by size:
2758 381 355 302 214
BsrBI CCG'CTC
Cuts at: 50 2302 2356 3840 4005 50
Size: 2252 54 1484 165 55
Fragments arranged by size:
2252 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 564 1922 564
Size: 1358 2652
BsrFI r'CCGG_y
Cuts at: 2008 2189 2398 2008
Size: 181 209 3620
Fragments arranged by size:
3620 209 181
BssHII G'CGCG_C
Cuts at: 254 322 368 254
Size: 68 46 3896
Fragments arranged by size:
3896 68 46
BssSI C'ACGA_G
Cuts at: 634 757 2281 2639 634
Size: 123 1524 358 2005
Fragments arranged by size:
2005 1524 358 123
BstAPI GCAn_nnn'nTGC
Cuts at: 1387 1387
Size: 4010
BstBI TT'CG_AA
Cuts at: 2371 2371
Size: 4010
BstYI r'GATC_y
Cuts at: 1 151 992 1035 1502 1525 1860 2106 3118
Size: 150 841 43 467 23 335 246 1012
Cuts at: 3118 3204 3216 3562 1
Size: 86 12 346 449
Fragments arranged by size:
1012 841 467 449 346 335 246 150
86 43 23 12
BtgI C'CryG_G
Cuts at: 128 220 362 128
Size: 92 142 3776
Fragments arranged by size:
3776 142 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1960 2141 2304 1960
Size: 181 163 3666
Fragments arranged by size:
3666 181 163
BtsI GCAGTG_nn'
Cuts at: 328 1253 328
Size: 925 3085
DraI TTT'AAA
Cuts at: 1009 1009
Size: 4010
DrdI GACnn_nn'nnGTC
Cuts at: 1716 2574 1716
Size: 858 3152
EaeI y'GGCC_r
Cuts at: 388 1595 1769 2160 2187 3532 388
Size: 1207 174 391 27 1345 866
Fragments arranged by size:
1345 1207 866 391 174 27
EagI C'GGCC_G
Cuts at: 388 1595 388
Size: 1207 2803
EarI CTCTTCn'nnn_
Cuts at: 977 2033 2243 977
Size: 1056 210 2744
Fragments arranged by size:
2744 1056 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2218 2528 2674 3820 2218
Size: 310 146 1146 2408
Fragments arranged by size:
2408 1146 310 146
EcoICRI GAG'CTC
Cuts at: 125 852 1022 125
Size: 727 170 3113
Fragments arranged by size:
3113 727 170
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 571 664 868 1835 2267 3014 3282 235
Size: 336 93 204 967 432 747 268 963
Fragments arranged by size:
967 963 747 432 336 268 204 93
EcoO109I rG'GnC_Cy
Cuts at: 233 546 790 1149 233
Size: 313 244 359 3094
Fragments arranged by size:
3094 359 313 244
EcoRI G'AATT_C
Cuts at: 1014 1014
Size: 4010
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2029 2029
Size: 4010
FspI TGC'GCA
Cuts at: 1791 1791
Size: 4010
HaeII r_GCGC'y
Cuts at: 146 277 351 380 621 1444 1692 2714 3693
Size: 131 74 29 241 823 248 1022 979
Cuts at: 3693 146
Size: 463
Fragments arranged by size:
1022 979 823 463 248 241 131 74
29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 466 498 1852 1882 1884 1914 466
Size: 32 1354 30 2 30 2562
Fragments arranged by size:
2562 1354 32 30 30 2
HincII GTy'rAC
Cuts at: 1049 1049
Size: 4010
KasI G'GCGC_C
Cuts at: 1440 1688 1440
Size: 248 3762
KpnI G_GTAC'C
Cuts at: 1030 1030
Size: 4010
MluI A'CGCG_T
Cuts at: 3443 3443
Size: 4010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 170 2681 2865 170
Size: 2511 184 1315
Fragments arranged by size:
2511 1315 184
MscI TGG'CCA
Cuts at: 1771 1771
Size: 4010
MslI CAynn'nnrTG
Cuts at: 1999 2126 1999
Size: 127 3883
MspA1I CmG'CkG
Cuts at: 130 182 222 364 492 627 677 1121 1435
Size: 52 40 142 128 135 50 444 314
Cuts at: 1435 1795 2808 3053 130
Size: 360 1013 245 1087
Fragments arranged by size:
1087 1013 444 360 314 245 142 135
128 52 50 40
NaeI GCC'GGC
Cuts at: 2191 2191
Size: 4010
NarI GG'CG_CC
Cuts at: 1441 1689 1441
Size: 248 3762
NgoMIV G'CCGG_C
Cuts at: 2189 2189
Size: 4010
NspI r_CATG'y
Cuts at: 944 966 1063 1563 2470 944
Size: 22 97 500 907 2484
Fragments arranged by size:
2484 907 500 97 22
PasI CC'CwG_GG
Cuts at: 147 238 432 147
Size: 91 194 3725
Fragments arranged by size:
3725 194 91
PciI A'CATG_T
Cuts at: 940 2466 940
Size: 1526 2484
PflMI CCAn_nnn'nTGG
Cuts at: 549 549
Size: 4010
PfoI T'CCnGG_A
Cuts at: 154 154
Size: 4010
PmeI GTTT'AAAC
Cuts at: 1009 1009
Size: 4010
PmlI CAC'GTG
Cuts at: 498 498
Size: 4010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 3978
PpuMI rG'GwC_Cy
Cuts at: 546 546
Size: 4010
PspOMI G'GGCC_C
Cuts at: 781 781
Size: 4010
PstI C_TGCA'G
Cuts at: 400 538 626 1057 1742 400
Size: 138 88 431 685 2668
Fragments arranged by size:
2668 685 431 138 88
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4010
PvuII CAG'CTG
Cuts at: 182 1435 1795 182
Size: 1253 360 2397
Fragments arranged by size:
2397 1253 360
RsrII CG'GwC_CG
Cuts at: 2205 2205
Size: 4010
SacI G_AGCT'C
Cuts at: 127 854 1024 127
Size: 727 170 3113
Fragments arranged by size:
3113 727 170
SacII CC_GC'GG
Cuts at: 131 223 365 131
Size: 92 142 3776
Fragments arranged by size:
3776 142 92
SalI G'TCGA_C
Cuts at: 1047 1047
Size: 4010
SapI GCTCTTCn'nnn_
Cuts at: 2033 2243 2033
Size: 210 3800
SbfI CC_TGCA'GG
Cuts at: 1057 1057
Size: 4010
ScaI AGT'ACT
Cuts at: 904 3527 904
Size: 2623 1387
SfcI C'TryA_G
Cuts at: 33 228 396 534 622 930 1053 1738 2731
Size: 195 168 138 88 308 123 685 993
Cuts at: 2731 2922 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 308 195 191 168 138
123 88
SfoI GGC'GCC
Cuts at: 1442 1690 1442
Size: 248 3762
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
SmaI CCC'GGG
Cuts at: 248 1032 248
Size: 784 3226
SmlI C'TyrA_G
Cuts at: 1165 2080 2572 2834 1165
Size: 915 492 262 2341
Fragments arranged by size:
2341 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4010
SphI G_CATG'C
Cuts at: 1063 1563 1063
Size: 500 3510
SrfI GCCC'GGGC
Cuts at: 248 248
Size: 4010
StyI C'CwwG_G
Cuts at: 666 1144 1498 666
Size: 478 354 3178
Fragments arranged by size:
3178 478 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1593 1726 2185 2394 1593
Size: 133 459 209 3209
Fragments arranged by size:
3209 459 209 133
TatI w'GTAC_w
Cuts at: 902 3525 902
Size: 2623 1387
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 3978
Tth111I GACn'n_nGTC
Cuts at: 1807 1807
Size: 4010
XbaI T'CTAG_A
Cuts at: 1041 1041
Size: 4010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1505 1505
Size: 4010
XmaI C'CCGG_G
Cuts at: 246 1030 246
Size: 784 3226
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AvrII BaeI BbsI
BfrBI BmgBI BmtI BsaXI BseRI BsiWI BsmI BsrGI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PshAI PsiI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SspI StuI
SwaI XhoI XmnI ZraI