Restriction map for
pF1KB9816
(Circular) (Six-base) MAPSORT of: pF1KB9816.seq Check: 4001 from: 1 to: 4013
pF1KB9816 4013 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 236 1051 236
Size: 815 3198
Acc65I G'GTAC_C
Cuts at: 1029 1029
Size: 4013
AclI AA'CG_TT
Cuts at: 3853 3853
Size: 4013
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 642 982 1838 2270 3017 3285 310
Size: 332 340 856 432 747 268 1038
Fragments arranged by size:
1038 856 747 432 340 332 268
AflIII A'CryG_T
Cuts at: 1365 2469 3446 1365
Size: 1104 977 1932
Fragments arranged by size:
1932 1104 977
AgeI A'CCGG_T
Cuts at: 934 2401 934
Size: 1467 2546
AlwNI CAG_nnn'CTG
Cuts at: 1318 2885 1318
Size: 1567 2446
ApaI G_GGCC'C
Cuts at: 504 795 504
Size: 291 3722
ApaLI G'TGCA_C
Cuts at: 2783 2783
Size: 4013
ApoI r'AATT_y
Cuts at: 1017 3366 3622 1017
Size: 2349 256 1408
Fragments arranged by size:
2349 1408 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4013
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4013
AvaI C'yCGr_G
Cuts at: 1033 1033
Size: 4013
BamHI G'GATC_C
Cuts at: 1038 1038
Size: 4013
BanI G'GyrC_C
Cuts at: 901 1029 1443 1691 1726 901
Size: 128 414 248 35 3188
Fragments arranged by size:
3188 414 248 128 35
BanII G_rGCy'C
Cuts at: 504 795 893 1027 2057 504
Size: 291 98 134 1030 2460
Fragments arranged by size:
2460 1030 291 134 98
BbeI G_GCGC'C
Cuts at: 1447 1695 1447
Size: 248 3765
BbsI GAAGACnn'nnnn_
Cuts at: 342 970 342
Size: 628 3385
BciVI GTATCCnnnnn_n'
Cuts at: 1911 2672 4013 1911
Size: 761 1341 1911
Fragments arranged by size:
1911 1341 761
BclI T'GATC_A
Cuts at: 1533 1533
Size: 4013
BglI GCCn_nnn'nGGC
Cuts at: 316 499 649 781 790 316
Size: 183 150 132 9 3539
Fragments arranged by size:
3539 183 150 132 9
BglII A'GATC_T
Cuts at: 1528 3565 1528
Size: 2037 1976
BlpI GC'TnA_GC
Cuts at: 1125 1125
Size: 4013
Bme1580I G_kGCm'C
Cuts at: 346 504 732 795 1638 1731 2787 346
Size: 158 228 63 843 93 1056 1572
Fragments arranged by size:
1572 1056 843 228 158 93 63
BmrI ACTGGGnnnn_n'
Cuts at: 1225 1306 1641 3785 1225
Size: 81 335 2144 1453
Fragments arranged by size:
2144 1453 335 81
BmtI G_CTAG'C
Cuts at: 310 310
Size: 4013
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2380 2380
Size: 4013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 640 927 530
Size: 110 287 3616
Fragments arranged by size:
3616 287 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1189 2068 2560 2858 1189
Size: 879 492 298 2344
Fragments arranged by size:
2344 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3717 3717
Size: 4013
BsaAI yAC'GTr
Cuts at: 714 1366 714
Size: 652 3361
BsaBI GATnn'nnATC
Cuts at: 6 1527 3564 6
Size: 1521 2037 455
Fragments arranged by size:
2037 1521 455
BsaHI Gr'CG_yC
Cuts at: 1444 1692 2394 3890 1444
Size: 248 702 1496 1567
Fragments arranged by size:
1567 1496 702 248
BsaWI w'CCGG_w
Cuts at: 458 609 934 1202 1723 2401 2675 2822 3233
Size: 151 325 268 521 678 274 147 411
Cuts at: 3233 458
Size: 1238
Fragments arranged by size:
1238 678 521 411 325 274 268 151
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 817 847 817
Size: 30 3983
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 787 787
Size: 4013
BseYI C'CCAG_C
Cuts at: 159 885 1438 2196 2773 3269 159
Size: 726 553 758 577 496 903
Fragments arranged by size:
903 758 726 577 553 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 431
Size: 4013
BsiEI CG_ry'CG
Cuts at: 104 1555 1601 2809 104
Size: 1451 46 1208 1308
Fragments arranged by size:
1451 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1027 1805 1995 2787 1027
Size: 778 190 792 2253
Fragments arranged by size:
2253 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 223 223
Size: 4013
Bsp1286I G_dGCh'C
Cuts at: 346 504 732 795 893 1027 1638 1731 1805
Size: 158 228 63 98 134 611 93 74
Cuts at: 1805 1995 2057 2787 346
Size: 190 62 730 1572
Fragments arranged by size:
1572 730 611 228 190 158 134 98
93 74 63 62
BspEI T'CCGG_A
Cuts at: 458 3233 458
Size: 2775 1238
BspHI T'CATG_A
Cuts at: 3189 3189
Size: 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 122 272 1063 1277 1579 1960 122
Size: 150 791 214 302 381 2175
Fragments arranged by size:
2175 791 381 302 214 150
BsrBI CCG'CTC
Cuts at: 50 171 740 2305 2359 3843 4008 50
Size: 121 569 1565 54 1484 165 55
Fragments arranged by size:
1565 1484 569 165 121 55 54
BsrDI GCAATG_nn'
Cuts at: 1925 1925
Size: 4013
BsrFI r'CCGG_y
Cuts at: 330 382 594 643 784 934 2011 2192 2401
Size: 52 212 49 141 150 1077 181 209
Cuts at: 2401 330
Size: 1942
Fragments arranged by size:
1942 1077 212 209 181 150 141 52
49
BssSI C'ACGA_G
Cuts at: 2284 2642 2284
Size: 358 3655
BstAPI GCAn_nnn'nTGC
Cuts at: 1390 1390
Size: 4013
BstBI TT'CG_AA
Cuts at: 2374 2374
Size: 4013
BstEII G'GTnAC_C
Cuts at: 930 930
Size: 4013
BstXI CCAn_nnnn'nTGG
Cuts at: 554 554
Size: 4013
BstYI r'GATC_y
Cuts at: 1 1038 1505 1528 1863 2109 3121 3207 3219
Size: 1037 467 23 335 246 1012 86 12
Cuts at: 3219 3565 1
Size: 346 449
Fragments arranged by size:
1037 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4013
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1963 2144 2307 1963
Size: 181 163 3669
Fragments arranged by size:
3669 181 163
BtsI GCAGTG_nn'
Cuts at: 1256 1256
Size: 4013
DraI TTT'AAA
Cuts at: 1012 1012
Size: 4013
DrdI GACnn_nn'nnGTC
Cuts at: 1719 2577 1719
Size: 858 3155
EaeI y'GGCC_r
Cuts at: 146 182 398 491 692 782 842 1598 1772
Size: 36 216 93 201 90 60 756 174
Cuts at: 1772 2163 2190 3535 146
Size: 391 27 1345 624
Fragments arranged by size:
1345 756 624 391 216 201 174 93
90 60 36 27
EagI C'GGCC_G
Cuts at: 1598 1598
Size: 4013
EarI CTCTTCn'nnn_
Cuts at: 2036 2246 2036
Size: 210 3803
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 774 894 2221 2531 2677 3823 774
Size: 120 1327 310 146 1146 964
Fragments arranged by size:
1327 1146 964 310 146 120
EcoICRI GAG'CTC
Cuts at: 1025 1025
Size: 4013
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 530 640 642 927 982 1838 2270 3017
Size: 220 110 2 285 55 856 432 747
Cuts at: 3017 3285 310
Size: 268 1038
Fragments arranged by size:
1038 856 747 432 285 268 220 110
55 2
EcoNI CCTnn'n_nnAGG
Cuts at: 459 459
Size: 4013
EcoO109I rG'GnC_Cy
Cuts at: 317 501 630 791 792 820 952 1152 317
Size: 184 129 161 1 28 132 200 3178
Fragments arranged by size:
3178 200 184 161 132 129 28 1
EcoRI G'AATT_C
Cuts at: 1017 1017
Size: 4013
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 4013
FspI TGC'GCA
Cuts at: 1794 1794
Size: 4013
HaeII r_GCGC'y
Cuts at: 307 1447 1695 2717 3696 307
Size: 1140 248 1022 979 624
Fragments arranged by size:
1140 1022 979 624 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 264 296 622 654 1855 1885 1887 1917 264
Size: 32 326 32 1201 30 2 30 2360
Fragments arranged by size:
2360 1201 326 32 32 30 30 2
HincII GTy'rAC
Cuts at: 600 1052 600
Size: 452 3561
KasI G'GCGC_C
Cuts at: 1443 1691 1443
Size: 248 3765
KpnI G_GTAC'C
Cuts at: 1033 1033
Size: 4013
MluI A'CGCG_T
Cuts at: 3446 3446
Size: 4013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 284 518 860 2684 2868 284
Size: 234 342 1824 184 1429
Fragments arranged by size:
1824 1429 342 234 184
MscI TGG'CCA
Cuts at: 184 694 844 1774 184
Size: 510 150 930 2423
Fragments arranged by size:
2423 930 510 150
MslI CAynn'nnrTG
Cuts at: 2002 2129 2002
Size: 127 3886
MspA1I CmG'CkG
Cuts at: 927 1124 1438 1798 2811 3056 927
Size: 197 314 360 1013 245 1884
Fragments arranged by size:
1884 1013 360 314 245 197
NaeI GCC'GGC
Cuts at: 332 384 645 786 2194 332
Size: 52 261 141 1408 2151
Fragments arranged by size:
2151 1408 261 141 52
NarI GG'CG_CC
Cuts at: 1444 1692 1444
Size: 248 3765
NcoI C'CATG_G
Cuts at: 107 107
Size: 4013
NgoMIV G'CCGG_C
Cuts at: 330 382 643 784 2192 330
Size: 52 261 141 1408 2151
Fragments arranged by size:
2151 1408 261 141 52
NheI G'CTAG_C
Cuts at: 306 306
Size: 4013
NspI r_CATG'y
Cuts at: 1066 1566 2473 1066
Size: 500 907 2606
Fragments arranged by size:
2606 907 500
PasI CC'CwG_GG
Cuts at: 579 579
Size: 4013
PciI A'CATG_T
Cuts at: 2469 2469
Size: 4013
PfoI T'CCnGG_A
Cuts at: 546 546
Size: 4013
PmeI GTTT'AAAC
Cuts at: 1012 1012
Size: 4013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3152 3184 3152
Size: 32 3981
PpuMI rG'GwC_Cy
Cuts at: 630 820 952 630
Size: 190 132 3691
Fragments arranged by size:
3691 190 132
PspOMI G'GGCC_C
Cuts at: 500 791 500
Size: 291 3722
PstI C_TGCA'G
Cuts at: 1060 1745 1060
Size: 685 3328
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4013
PvuII CAG'CTG
Cuts at: 1438 1798 1438
Size: 360 3653
RsrII CG'GwC_CG
Cuts at: 2208 2208
Size: 4013
SacI G_AGCT'C
Cuts at: 1027 1027
Size: 4013
SalI G'TCGA_C
Cuts at: 1050 1050
Size: 4013
SanDI GG'GwC_CC
Cuts at: 630 630
Size: 4013
SapI GCTCTTCn'nnn_
Cuts at: 2036 2246 2036
Size: 210 3803
SbfI CC_TGCA'GG
Cuts at: 1060 1060
Size: 4013
ScaI AGT'ACT
Cuts at: 3530 3530
Size: 4013
SfcI C'TryA_G
Cuts at: 33 1056 1741 2734 2925 33
Size: 1023 685 993 191 1121
Fragments arranged by size:
1121 1023 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 499 790 499
Size: 291 3722
SfoI GGC'GCC
Cuts at: 1445 1693 1445
Size: 248 3765
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4013
SmaI CCC'GGG
Cuts at: 1035 1035
Size: 4013
SmlI C'TyrA_G
Cuts at: 1168 2083 2575 2837 1168
Size: 915 492 262 2344
Fragments arranged by size:
2344 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4013
SphI G_CATG'C
Cuts at: 1066 1566 1066
Size: 500 3513
StuI AGG'CCT
Cuts at: 625 625
Size: 4013
StyI C'CwwG_G
Cuts at: 107 440 689 1147 1501 107
Size: 333 249 458 354 2619
Fragments arranged by size:
2619 458 354 333 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 768 1596 1729 2188 2397 768
Size: 828 133 459 209 2384
Fragments arranged by size:
2384 828 459 209 133
TatI w'GTAC_w
Cuts at: 3528 3528
Size: 4013
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3530 3562 3530
Size: 32 3981
Tth111I GACn'n_nGTC
Cuts at: 1810 1810
Size: 4013
XbaI T'CTAG_A
Cuts at: 1044 1044
Size: 4013
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1508 1508
Size: 4013
XmaI C'CCGG_G
Cuts at: 1033 1033
Size: 4013
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI Bpu10I
BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PflMI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI