Restriction map for
pF1KB9818
(Circular) (Six-base) MAPSORT of: pF1KB9818.seq Check: 5903 from: 1 to: 4040
pF1KB9818 4040 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 634 855 634
Size: 221 3819
AccI GT'mk_AC
Cuts at: 1078 1078
Size: 4040
Acc65I G'GTAC_C
Cuts at: 1056 1056
Size: 4040
AclI AA'CG_TT
Cuts at: 3880 3880
Size: 4040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1865 2297 3044 3312 1865
Size: 432 747 268 2593
Fragments arranged by size:
2593 747 432 268
AflIII A'CryG_T
Cuts at: 1392 2496 3473 1392
Size: 1104 977 1959
Fragments arranged by size:
1959 1104 977
AgeI A'CCGG_T
Cuts at: 2428 2428
Size: 4040
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 382 414 382
Size: 32 4008
AlwNI CAG_nnn'CTG
Cuts at: 1345 2912 1345
Size: 1567 2473
ApaI G_GGCC'C
Cuts at: 918 918
Size: 4040
ApaLI G'TGCA_C
Cuts at: 2810 2810
Size: 4040
ApoI r'AATT_y
Cuts at: 1044 3393 3649 1044
Size: 2349 256 1435
Fragments arranged by size:
2349 1435 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
AvaI C'yCGr_G
Cuts at: 1060 1060
Size: 4040
AvrII C'CTAG_G
Cuts at: 630 630
Size: 4040
BamHI G'GATC_C
Cuts at: 1065 1065
Size: 4040
BanI G'GyrC_C
Cuts at: 229 243 867 1056 1470 1718 1753 229
Size: 14 624 189 414 248 35 2516
Fragments arranged by size:
2516 624 414 248 189 35 14
BanII G_rGCy'C
Cuts at: 812 918 1054 2084 812
Size: 106 136 1030 2768
Fragments arranged by size:
2768 1030 136 106
BbeI G_GCGC'C
Cuts at: 1474 1722 1474
Size: 248 3792
BciVI GTATCCnnnnn_n'
Cuts at: 394 1938 2699 4040 394
Size: 1544 761 1341 394
Fragments arranged by size:
1544 1341 761 394
BclI T'GATC_A
Cuts at: 956 1560 956
Size: 604 3436
BglI GCCn_nnn'nGGC
Cuts at: 157 157
Size: 4040
BglII A'GATC_T
Cuts at: 533 1555 3592 533
Size: 1022 2037 981
Fragments arranged by size:
2037 1022 981
BlpI GC'TnA_GC
Cuts at: 1152 1152
Size: 4040
Bme1580I G_kGCm'C
Cuts at: 232 248 918 1665 1758 2814 232
Size: 16 670 747 93 1056 1458
Fragments arranged by size:
1458 1056 747 670 93 16
BmgBI CAC'GTC
Cuts at: 582 582
Size: 4040
BmrI ACTGGGnnnn_n'
Cuts at: 329 1252 1333 1668 3812 329
Size: 923 81 335 2144 557
Fragments arranged by size:
2144 923 557 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 409 514 874 256
Size: 153 105 360 3422
Fragments arranged by size:
3422 360 153 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 175 967 1216 2095 2587 2885 136
Size: 39 792 249 879 492 298 1291
Fragments arranged by size:
1291 879 792 492 298 249 39
BsaI GGTCTCn'nnnn_
Cuts at: 640 3744 640
Size: 3104 936
BsaAI yAC'GTr
Cuts at: 1393 1393
Size: 4040
BsaBI GATnn'nnATC
Cuts at: 6 1554 3591 6
Size: 1548 2037 455
Fragments arranged by size:
2037 1548 455
BsaHI Gr'CG_yC
Cuts at: 1471 1719 2421 3917 1471
Size: 248 702 1496 1594
Fragments arranged by size:
1594 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1229 1750 2428 2702 2849 3260 1229
Size: 521 678 274 147 411 2009
Fragments arranged by size:
2009 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 252 282 382 412 252
Size: 30 100 30 3880
Fragments arranged by size:
3880 100 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 138 195 198 201 206 253 376 439 754
Size: 57 3 3 5 47 123 63 315
Cuts at: 754 769 1033 138
Size: 15 264 3145
Fragments arranged by size:
3145 315 264 123 63 57 47 15
5 3 3
BseYI C'CCAG_C
Cuts at: 253 654 1465 2223 2800 3296 253
Size: 401 811 758 577 496 997
Fragments arranged by size:
997 811 758 577 496 401
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 211 859 115
Size: 96 648 3296
Fragments arranged by size:
3296 648 96
BsiEI CG_ry'CG
Cuts at: 104 1582 1628 2836 104
Size: 1478 46 1208 1308
Fragments arranged by size:
1478 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 794 1054 1832 2022 2814 794
Size: 260 778 190 792 2020
Fragments arranged by size:
2020 792 778 260 190
Bsp1286I G_dGCh'C
Cuts at: 232 248 794 812 918 1054 1665 1758 1832
Size: 16 546 18 106 136 611 93 74
Cuts at: 1832 2022 2084 2814 232
Size: 190 62 730 1458
Fragments arranged by size:
1458 730 611 546 190 136 106 93
74 62 18 16
BspEI T'CCGG_A
Cuts at: 3260 3260
Size: 4040
BspHI T'CATG_A
Cuts at: 707 3216 707
Size: 2509 1531
BspMI ACCTGCnnnn'nnnn_
Cuts at: 634 855 1090 1304 1606 1987 634
Size: 221 235 214 302 381 2687
Fragments arranged by size:
2687 381 302 235 221 214
BsrBI CCG'CTC
Cuts at: 50 2332 2386 3870 4035 50
Size: 2282 54 1484 165 55
Fragments arranged by size:
2282 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 722 1952 722
Size: 1230 2810
BsrFI r'CCGG_y
Cuts at: 601 860 2038 2219 2428 601
Size: 259 1178 181 209 2213
Fragments arranged by size:
2213 1178 259 209 181
BssSI C'ACGA_G
Cuts at: 2311 2669 2311
Size: 358 3682
BstAPI GCAn_nnn'nTGC
Cuts at: 1417 1417
Size: 4040
BstBI TT'CG_AA
Cuts at: 2401 2401
Size: 4040
BstYI r'GATC_y
Cuts at: 1 264 533 1065 1532 1555 1890 2136 3148
Size: 263 269 532 467 23 335 246 1012
Cuts at: 3148 3234 3246 3592 1
Size: 86 12 346 449
Fragments arranged by size:
1012 532 467 449 346 335 269 263
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 143 281 737 143
Size: 138 456 3446
Fragments arranged by size:
3446 456 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1990 2171 2334 1990
Size: 181 163 3696
Fragments arranged by size:
3696 181 163
BtsI GCAGTG_nn'
Cuts at: 132 248 1283 132
Size: 116 1035 2889
Fragments arranged by size:
2889 1035 116
DraI TTT'AAA
Cuts at: 1039 1039
Size: 4040
DrdI GACnn_nn'nnGTC
Cuts at: 1746 2604 1746
Size: 858 3182
EaeI y'GGCC_r
Cuts at: 603 726 1625 1799 2190 2217 3562 603
Size: 123 899 174 391 27 1345 1081
Fragments arranged by size:
1345 1081 899 391 174 123 27
EagI C'GGCC_G
Cuts at: 1625 1625
Size: 4040
EarI CTCTTCn'nnn_
Cuts at: 353 1019 2063 2273 353
Size: 666 1044 210 2120
Fragments arranged by size:
2120 1044 666 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 282 2248 2558 2704 3850 282
Size: 1966 310 146 1146 472
Fragments arranged by size:
1966 1146 472 310 146
EcoICRI GAG'CTC
Cuts at: 1052 1052
Size: 4040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 409 514 874 1865 2297 3044 3312 256
Size: 153 105 360 991 432 747 268 984
Fragments arranged by size:
991 984 747 432 360 268 153 105
EcoNI CCTnn'n_nnAGG
Cuts at: 238 238
Size: 4040
EcoO109I rG'GnC_Cy
Cuts at: 171 914 915 1179 171
Size: 743 1 264 3032
Fragments arranged by size:
3032 743 264 1
EcoRI G'AATT_C
Cuts at: 1044 1044
Size: 4040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 395 702 2059 395
Size: 307 1357 2376
Fragments arranged by size:
2376 1357 307
FspI TGC'GCA
Cuts at: 1821 1821
Size: 4040
HaeII r_GCGC'y
Cuts at: 1474 1722 2744 3723 1474
Size: 248 1022 979 1791
Fragments arranged by size:
1791 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 395 427 1882 1912 1914 1944 395
Size: 32 1455 30 2 30 2491
Fragments arranged by size:
2491 1455 32 30 30 2
HincII GTy'rAC
Cuts at: 260 1079 260
Size: 819 3221
KasI G'GCGC_C
Cuts at: 1470 1718 1470
Size: 248 3792
KpnI G_GTAC'C
Cuts at: 1060 1060
Size: 4040
MluI A'CGCG_T
Cuts at: 3473 3473
Size: 4040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2711 2895 2711
Size: 184 3856
MscI TGG'CCA
Cuts at: 1801 1801
Size: 4040
MslI CAynn'nnrTG
Cuts at: 580 611 2029 2156 580
Size: 31 1418 127 2464
Fragments arranged by size:
2464 1418 127 31
MspA1I CmG'CkG
Cuts at: 564 1151 1465 1825 2838 3083 564
Size: 587 314 360 1013 245 1521
Fragments arranged by size:
1521 1013 587 360 314 245
NaeI GCC'GGC
Cuts at: 2221 2221
Size: 4040
NarI GG'CG_CC
Cuts at: 1471 1719 1471
Size: 248 3792
NgoMIV G'CCGG_C
Cuts at: 2219 2219
Size: 4040
NspI r_CATG'y
Cuts at: 1093 1593 2500 1093
Size: 500 907 2633
Fragments arranged by size:
2633 907 500
PasI CC'CwG_GG
Cuts at: 174 174
Size: 4040
PciI A'CATG_T
Cuts at: 2496 2496
Size: 4040
PflMI CCAn_nnn'nTGG
Cuts at: 834 834
Size: 4040
PfoI T'CCnGG_A
Cuts at: 386 386
Size: 4040
PmeI GTTT'AAAC
Cuts at: 1039 1039
Size: 4040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 382 414 3179 3211 382
Size: 32 2765 32 1211
Fragments arranged by size:
2765 1211 32 32
PspOMI G'GGCC_C
Cuts at: 914 914
Size: 4040
PstI C_TGCA'G
Cuts at: 1087 1772 1087
Size: 685 3355
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4040
PvuII CAG'CTG
Cuts at: 564 1465 1825 564
Size: 901 360 2779
Fragments arranged by size:
2779 901 360
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 4040
SacI G_AGCT'C
Cuts at: 1054 1054
Size: 4040
SalI G'TCGA_C
Cuts at: 1077 1077
Size: 4040
SapI GCTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
SbfI CC_TGCA'GG
Cuts at: 1087 1087
Size: 4040
ScaI AGT'ACT
Cuts at: 3557 3557
Size: 4040
SfcI C'TryA_G
Cuts at: 33 1083 1768 2761 2952 33
Size: 1050 685 993 191 1121
Fragments arranged by size:
1121 1050 993 685 191
SfoI GGC'GCC
Cuts at: 1472 1720 1472
Size: 248 3792
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
SmaI CCC'GGG
Cuts at: 1062 1062
Size: 4040
SmlI C'TyrA_G
Cuts at: 115 154 946 1195 2110 2602 2864 115
Size: 39 792 249 915 492 262 1291
Fragments arranged by size:
1291 915 792 492 262 249 39
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4040
SphI G_CATG'C
Cuts at: 1093 1593 1093
Size: 500 3540
StyI C'CwwG_G
Cuts at: 630 910 1174 1528 630
Size: 280 264 354 3142
Fragments arranged by size:
3142 354 280 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 646 841 1623 1756 2215 2424 646
Size: 195 782 133 459 209 2262
Fragments arranged by size:
2262 782 459 209 195 133
TatI w'GTAC_w
Cuts at: 3555 3555
Size: 4040
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3557 3589 3557
Size: 32 4008
Tth111I GACn'n_nGTC
Cuts at: 1837 1837
Size: 4040
XbaI T'CTAG_A
Cuts at: 1071 1071
Size: 4040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1535 1535
Size: 4040
XmaI C'CCGG_G
Cuts at: 1060 1060
Size: 4040
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI BaeI
BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I BtgI ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI