Restriction map for
pF1KB9825
(Circular) (Six-base) MAPSORT of: pF1KB9825.seq Check: 6902 from: 1 to: 4064
pF1KB9825 4064 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 658 658
Size: 4064
AccI GT'mk_AC
Cuts at: 1102 1102
Size: 4064
Acc65I G'GTAC_C
Cuts at: 891 1080 891
Size: 189 3875
AclI AA'CG_TT
Cuts at: 3904 3904
Size: 4064
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 1889 2321 3068 3336 604
Size: 1285 432 747 268 1332
Fragments arranged by size:
1332 1285 747 432 268
AfeI AGC'GCT
Cuts at: 544 544
Size: 4064
AflIII A'CryG_T
Cuts at: 1416 2520 3497 1416
Size: 1104 977 1983
Fragments arranged by size:
1983 1104 977
AgeI A'CCGG_T
Cuts at: 2452 2452
Size: 4064
AhdI GACnn_n'nnGTC
Cuts at: 414 414
Size: 4064
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 236 236
Size: 4064
AlwNI CAG_nnn'CTG
Cuts at: 1369 2936 1369
Size: 1567 2497
ApaLI G'TGCA_C
Cuts at: 2834 2834
Size: 4064
ApoI r'AATT_y
Cuts at: 1068 3417 3673 1068
Size: 2349 256 1459
Fragments arranged by size:
2349 1459 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4064
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
AvaI C'yCGr_G
Cuts at: 1084 1084
Size: 4064
BamHI G'GATC_C
Cuts at: 1089 1089
Size: 4064
BanI G'GyrC_C
Cuts at: 253 891 1080 1494 1742 1777 253
Size: 638 189 414 248 35 2540
Fragments arranged by size:
2540 638 414 248 189 35
BanII G_rGCy'C
Cuts at: 836 942 1078 2108 836
Size: 106 136 1030 2792
Fragments arranged by size:
2792 1030 136 106
BbeI G_GCGC'C
Cuts at: 1498 1746 1498
Size: 248 3816
BbsI GAAGACnn'nnnn_
Cuts at: 104 104
Size: 4064
BciVI GTATCCnnnnn_n'
Cuts at: 1962 2723 4064 1962
Size: 761 1341 1962
Fragments arranged by size:
1962 1341 761
BclI T'GATC_A
Cuts at: 1584 1584
Size: 4064
BglII A'GATC_T
Cuts at: 692 1579 3616 692
Size: 887 2037 1140
Fragments arranged by size:
2037 1140 887
BlpI GC'TnA_GC
Cuts at: 1176 1176
Size: 4064
Bme1580I G_kGCm'C
Cuts at: 733 1689 1782 2838 733
Size: 956 93 1056 1959
Fragments arranged by size:
1959 1056 956 93
BmgBI CAC'GTC
Cuts at: 606 606
Size: 4064
BmrI ACTGGGnnnn_n'
Cuts at: 1276 1357 1692 3836 1276
Size: 81 335 2144 1504
Fragments arranged by size:
2144 1504 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 451 2431 451
Size: 1980 2084
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 369 538 898 991 280
Size: 89 169 360 93 3353
Fragments arranged by size:
3353 360 169 93 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1240 2119 2611 2909 1240
Size: 879 492 298 2395
Fragments arranged by size:
2395 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3768 3768
Size: 4064
BsaAI yAC'GTr
Cuts at: 1417 1417
Size: 4064
BsaBI GATnn'nnATC
Cuts at: 6 1578 3615 6
Size: 1572 2037 455
Fragments arranged by size:
2037 1572 455
BsaHI Gr'CG_yC
Cuts at: 1495 1743 2445 3941 1495
Size: 248 702 1496 1618
Fragments arranged by size:
1618 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1253 1774 2452 2726 2873 3284 1253
Size: 521 678 274 147 411 2033
Fragments arranged by size:
2033 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 219 222 223 225 230 400 778 793 1057
Size: 3 1 2 5 170 378 15 264
Cuts at: 1057 219
Size: 3226
Fragments arranged by size:
3226 378 264 170 15 5 3 2
1
BseYI C'CCAG_C
Cuts at: 678 783 1489 2247 2824 3320 678
Size: 105 706 758 577 496 1422
Fragments arranged by size:
1422 758 706 577 496 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 883
Size: 4064
BsiEI CG_ry'CG
Cuts at: 104 1606 1652 2860 104
Size: 1502 46 1208 1308
Fragments arranged by size:
1502 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 818 1078 1856 2046 2838 818
Size: 260 778 190 792 2044
Fragments arranged by size:
2044 792 778 260 190
Bsp1286I G_dGCh'C
Cuts at: 733 818 836 942 1078 1689 1782 1856 2046
Size: 85 18 106 136 611 93 74 190
Cuts at: 2046 2108 2838 733
Size: 62 730 1959
Fragments arranged by size:
1959 730 611 190 136 106 93 85
74 62 18
BspEI T'CCGG_A
Cuts at: 3284 3284
Size: 4064
BspHI T'CATG_A
Cuts at: 3240 3240
Size: 4064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 277 658 879 1114 1328 1630 2011 277
Size: 381 221 235 214 302 381 2330
Fragments arranged by size:
2330 381 381 302 235 221 214
BsrBI CCG'CTC
Cuts at: 50 2356 2410 3894 4059 50
Size: 2306 54 1484 165 55
Fragments arranged by size:
2306 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 746 1004 1976 746
Size: 258 972 2834
Fragments arranged by size:
2834 972 258
BsrFI r'CCGG_y
Cuts at: 884 2062 2243 2452 884
Size: 1178 181 209 2496
Fragments arranged by size:
2496 1178 209 181
BssHII G'CGCG_C
Cuts at: 910 910
Size: 4064
BssSI C'ACGA_G
Cuts at: 2335 2693 2335
Size: 358 3706
BstAPI GCAn_nnn'nTGC
Cuts at: 1441 1441
Size: 4064
BstBI TT'CG_AA
Cuts at: 2425 2425
Size: 4064
BstXI CCAn_nnnn'nTGG
Cuts at: 495 495
Size: 4064
BstYI r'GATC_y
Cuts at: 1 692 1089 1556 1579 1914 2160 3172 3258
Size: 691 397 467 23 335 246 1012 86
Cuts at: 3258 3270 3616 1
Size: 12 346 449
Fragments arranged by size:
1012 691 467 449 397 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 143 305 143
Size: 162 3902
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2014 2195 2358 2014
Size: 181 163 3720
Fragments arranged by size:
3720 181 163
BtsI GCAGTG_nn'
Cuts at: 132 1307 132
Size: 1175 2889
DraI TTT'AAA
Cuts at: 1063 1063
Size: 4064
DrdI GACnn_nn'nnGTC
Cuts at: 1770 2628 1770
Size: 858 3206
EaeI y'GGCC_r
Cuts at: 1649 1823 2214 2241 3586 1649
Size: 174 391 27 1345 2127
Fragments arranged by size:
2127 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1649 1649
Size: 4064
EarI CTCTTCn'nnn_
Cuts at: 118 161 377 1043 2087 2297 118
Size: 43 216 666 1044 210 1885
Fragments arranged by size:
1885 1044 666 216 210 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2272 2582 2728 3874 2272
Size: 310 146 1146 2462
Fragments arranged by size:
2462 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1076 1076
Size: 4064
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 369 538 604 898 991 1889 2321 3068
Size: 89 169 66 294 93 898 432 747
Cuts at: 3068 3336 280
Size: 268 1008
Fragments arranged by size:
1008 898 747 432 294 268 169 93
89 66
EcoNI CCTnn'n_nnAGG
Cuts at: 146 146
Size: 4064
EcoO109I rG'GnC_Cy
Cuts at: 171 289 1203 171
Size: 118 914 3032
Fragments arranged by size:
3032 914 118
EcoRI G'AATT_C
Cuts at: 1068 1068
Size: 4064
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 564 609 2083 564
Size: 45 1474 2545
Fragments arranged by size:
2545 1474 45
FspI TGC'GCA
Cuts at: 191 1845 191
Size: 1654 2410
HaeII r_GCGC'y
Cuts at: 546 758 1498 1746 2768 3747 546
Size: 212 740 248 1022 979 863
Fragments arranged by size:
1022 979 863 740 248 212
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 400 432 742 774 1906 1936 1938 1968 400
Size: 32 310 32 1132 30 2 30 2496
Fragments arranged by size:
2496 1132 310 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1103 1103
Size: 4064
KasI G'GCGC_C
Cuts at: 1494 1742 1494
Size: 248 3816
KpnI G_GTAC'C
Cuts at: 895 1084 895
Size: 189 3875
MfeI C'AATT_G
Cuts at: 734 734
Size: 4064
MluI A'CGCG_T
Cuts at: 3497 3497
Size: 4064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2735 2919 2735
Size: 184 3880
MscI TGG'CCA
Cuts at: 1825 1825
Size: 4064
MslI CAynn'nnrTG
Cuts at: 604 2053 2180 604
Size: 1449 127 2488
Fragments arranged by size:
2488 1449 127
MspA1I CmG'CkG
Cuts at: 1175 1489 1849 2862 3107 1175
Size: 314 360 1013 245 2132
Fragments arranged by size:
2132 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2245 2245
Size: 4064
NarI GG'CG_CC
Cuts at: 1495 1743 1495
Size: 248 3816
NgoMIV G'CCGG_C
Cuts at: 2243 2243
Size: 4064
NspI r_CATG'y
Cuts at: 979 1117 1617 2524 979
Size: 138 500 907 2519
Fragments arranged by size:
2519 907 500 138
PasI CC'CwG_GG
Cuts at: 174 174
Size: 4064
PciI A'CATG_T
Cuts at: 2520 2520
Size: 4064
PfoI T'CCnGG_A
Cuts at: 968 968
Size: 4064
PmeI GTTT'AAAC
Cuts at: 1063 1063
Size: 4064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 406 438 3203 3235 406
Size: 32 2765 32 1235
Fragments arranged by size:
2765 1235 32 32
PpuMI rG'GwC_Cy
Cuts at: 289 289
Size: 4064
PstI C_TGCA'G
Cuts at: 1111 1796 1111
Size: 685 3379
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4064
PvuII CAG'CTG
Cuts at: 1489 1849 1489
Size: 360 3704
RsrII CG'GwC_CG
Cuts at: 2259 2259
Size: 4064
SacI G_AGCT'C
Cuts at: 1078 1078
Size: 4064
SalI G'TCGA_C
Cuts at: 1101 1101
Size: 4064
SapI GCTCTTCn'nnn_
Cuts at: 2087 2297 2087
Size: 210 3854
SbfI CC_TGCA'GG
Cuts at: 1111 1111
Size: 4064
ScaI AGT'ACT
Cuts at: 3581 3581
Size: 4064
SfcI C'TryA_G
Cuts at: 33 1107 1792 2785 2976 33
Size: 1074 685 993 191 1121
Fragments arranged by size:
1121 1074 993 685 191
SfoI GGC'GCC
Cuts at: 1496 1744 1496
Size: 248 3816
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
SmaI CCC'GGG
Cuts at: 1086 1086
Size: 4064
SmlI C'TyrA_G
Cuts at: 1219 2134 2626 2888 1219
Size: 915 492 262 2395
Fragments arranged by size:
2395 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4064
SphI G_CATG'C
Cuts at: 1117 1617 1117
Size: 500 3564
StuI AGG'CCT
Cuts at: 710 710
Size: 4064
StyI C'CwwG_G
Cuts at: 488 934 1198 1552 488
Size: 446 264 354 3000
Fragments arranged by size:
3000 446 354 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 175 775 849 865 1647 1780 2239 2448 175
Size: 600 74 16 782 133 459 209 1791
Fragments arranged by size:
1791 782 600 459 209 133 74 16
TatI w'GTAC_w
Cuts at: 3579 3579
Size: 4064
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3581 3613 3581
Size: 32 4032
Tth111I GACn'n_nGTC
Cuts at: 1861 1861
Size: 4064
XbaI T'CTAG_A
Cuts at: 1095 1095
Size: 4064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1559 1559
Size: 4064
XmaI C'CCGG_G
Cuts at: 1084 1084
Size: 4064
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AleI AloI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmtI Bpu10I BsaXI BsiWI
BsmI BsmBI BsrGI BstEII BstZ17I BtgI ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI NcoI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI