Restriction map for
pF1KB9829
(Circular) (Six-base) MAPSORT of: pF1KB9829.seq Check: 5001 from: 1 to: 4133
pF1KB9829 4133 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 432 432
Size: 4133
AccI GT'mk_AC
Cuts at: 723 1171 723
Size: 448 3685
Acc65I G'GTAC_C
Cuts at: 1149 1149
Size: 4133
AclI AA'CG_TT
Cuts at: 3973 3973
Size: 4133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1958 2390 3137 3405 1958
Size: 432 747 268 2686
Fragments arranged by size:
2686 747 432 268
AflIII A'CryG_T
Cuts at: 385 1485 2589 3566 385
Size: 1100 1104 977 952
Fragments arranged by size:
1104 1100 977 952
AgeI A'CCGG_T
Cuts at: 2521 2521
Size: 4133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 265 388 464 265
Size: 123 76 3934
Fragments arranged by size:
3934 123 76
AlwNI CAG_nnn'CTG
Cuts at: 1438 3005 1438
Size: 1567 2566
ApaI G_GGCC'C
Cuts at: 280 888 280
Size: 608 3525
ApaLI G'TGCA_C
Cuts at: 2903 2903
Size: 4133
ApoI r'AATT_y
Cuts at: 1137 3486 3742 1137
Size: 2349 256 1528
Fragments arranged by size:
2349 1528 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4133
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4133
AvaI C'yCGr_G
Cuts at: 256 1153 256
Size: 897 3236
BamHI G'GATC_C
Cuts at: 1158 1158
Size: 4133
BanI G'GyrC_C
Cuts at: 496 811 849 1149 1563 1811 1846 496
Size: 315 38 300 414 248 35 2783
Fragments arranged by size:
2783 414 315 300 248 38 35
BanII G_rGCy'C
Cuts at: 180 190 280 888 1147 2177 180
Size: 10 90 608 259 1030 2136
Fragments arranged by size:
2136 1030 608 259 90 10
BbeI G_GCGC'C
Cuts at: 1567 1815 1567
Size: 248 3885
BbsI GAAGACnn'nnnn_
Cuts at: 268 388 540 636 268
Size: 120 152 96 3765
Fragments arranged by size:
3765 152 120 96
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4099
BciVI GTATCCnnnnn_n'
Cuts at: 2031 2792 4133 2031
Size: 761 1341 2031
Fragments arranged by size:
2031 1341 761
BclI T'GATC_A
Cuts at: 152 1653 152
Size: 1501 2632
BglI GCCn_nnn'nGGC
Cuts at: 117 536 117
Size: 419 3714
BglII A'GATC_T
Cuts at: 1648 3685 1648
Size: 2037 2096
BlpI GC'TnA_GC
Cuts at: 1245 1245
Size: 4133
Bme1580I G_kGCm'C
Cuts at: 280 501 816 854 888 1758 1851 2907 280
Size: 221 315 38 34 870 93 1056 1506
Fragments arranged by size:
1506 1056 870 315 221 93 38 34
BmrI ACTGGGnnnn_n'
Cuts at: 1345 1426 1761 3905 1345
Size: 81 335 2144 1573
Fragments arranged by size:
2144 1573 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 654 2500 654
Size: 1846 2287
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 850 850
Size: 4133
Bpu10I CC'TnA_GC
Cuts at: 224 452 629 224
Size: 228 177 3728
Fragments arranged by size:
3728 228 177
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1309 2188 2680 2978 1309
Size: 879 492 298 2464
Fragments arranged by size:
2464 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3837 3837
Size: 4133
BsaAI yAC'GTr
Cuts at: 388 1486 388
Size: 1098 3035
BsaBI GATnn'nnATC
Cuts at: 6 1647 3684 6
Size: 1641 2037 455
Fragments arranged by size:
2037 1641 455
BsaHI Gr'CG_yC
Cuts at: 1564 1812 2514 4010 1564
Size: 248 702 1496 1687
Fragments arranged by size:
1687 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1322 1843 2521 2795 2942 3353 1322
Size: 521 678 274 147 411 2102
Fragments arranged by size:
2102 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1087 1117 1087
Size: 30 4103
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 682 682
Size: 4133
BseYI C'CCAG_C
Cuts at: 166 228 531 562 1558 2316 2893 3389 166
Size: 62 303 31 996 758 577 496 910
Fragments arranged by size:
996 910 758 577 496 303 62 31
BsiEI CG_ry'CG
Cuts at: 104 1675 1721 2929 104
Size: 1571 46 1208 1308
Fragments arranged by size:
1571 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 473 573 1082 1147 1925 2115 2907 473
Size: 100 509 65 778 190 792 1699
Fragments arranged by size:
1699 792 778 509 190 100 65
BsmI GAATG_Cn'
Cuts at: 789 789
Size: 4133
Bsp1286I G_dGCh'C
Cuts at: 180 190 280 473 501 573 816 854 888
Size: 10 90 193 28 72 243 38 34
Cuts at: 888 1082 1147 1758 1851 1925 2115 2177 2907
Size: 194 65 611 93 74 190 62 730
Cuts at: 2907 180
Size: 1406
Fragments arranged by size:
1406 730 611 243 194 193 190 93
90 74 72 65 62 38 34 28
10
BspEI T'CCGG_A
Cuts at: 3353 3353
Size: 4133
BspHI T'CATG_A
Cuts at: 3309 3309
Size: 4133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 432 1183 1397 1699 2080 432
Size: 751 214 302 381 2485
Fragments arranged by size:
2485 751 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2425 2479 3963 4128 50
Size: 2375 54 1484 165 55
Fragments arranged by size:
2375 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 772 2045 772
Size: 1273 2860
BsrFI r'CCGG_y
Cuts at: 437 2131 2312 2521 437
Size: 1694 181 209 2049
Fragments arranged by size:
2049 1694 209 181
BssSI C'ACGA_G
Cuts at: 2404 2762 2404
Size: 358 3775
BstAPI GCAn_nnn'nTGC
Cuts at: 774 1510 774
Size: 736 3397
BstBI TT'CG_AA
Cuts at: 2494 2494
Size: 4133
BstXI CCAn_nnnn'nTGG
Cuts at: 744 744
Size: 4133
BstYI r'GATC_y
Cuts at: 1 1158 1625 1648 1983 2229 3241 3327 3339
Size: 1157 467 23 335 246 1012 86 12
Cuts at: 3339 3685 1
Size: 346 449
Fragments arranged by size:
1157 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 281 281
Size: 4133
BtgI C'CryG_G
Cuts at: 158 158
Size: 4133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2083 2264 2427 2083
Size: 181 163 3789
Fragments arranged by size:
3789 181 163
BtsI GCAGTG_nn'
Cuts at: 324 1376 324
Size: 1052 3081
DraI TTT'AAA
Cuts at: 1132 1132
Size: 4133
DraIII CAC_nnn'GTG
Cuts at: 577 881 577
Size: 304 3829
DrdI GACnn_nn'nnGTC
Cuts at: 1839 2697 1839
Size: 858 3275
EaeI y'GGCC_r
Cuts at: 118 1718 1892 2283 2310 3655 118
Size: 1600 174 391 27 1345 596
Fragments arranged by size:
1600 1345 596 391 174 27
EagI C'GGCC_G
Cuts at: 1718 1718
Size: 4133
EarI CTCTTCn'nnn_
Cuts at: 1043 2156 2366 1043
Size: 1113 210 2810
Fragments arranged by size:
2810 1113 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2341 2651 2797 3943 2341
Size: 310 146 1146 2531
Fragments arranged by size:
2531 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1145 1145
Size: 4133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 850 1958 2390 3137 3405 850
Size: 1108 432 747 268 1578
Fragments arranged by size:
1578 1108 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 277 885 1018 1053 1272 277
Size: 608 133 35 219 3138
Fragments arranged by size:
3138 608 219 133 35
EcoRI G'AATT_C
Cuts at: 1137 1137
Size: 4133
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1072 2152 1072
Size: 1080 3053
FspI TGC'GCA
Cuts at: 1914 1914
Size: 4133
HaeII r_GCGC'y
Cuts at: 410 593 1095 1567 1815 2837 3816 410
Size: 183 502 472 248 1022 979 727
Fragments arranged by size:
1022 979 727 502 472 248 183
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1975 2005 2007 2037 1975
Size: 30 2 30 4071
Fragments arranged by size:
4071 30 30 2
HincII GTy'rAC
Cuts at: 724 1172 724
Size: 448 3685
HindIII A'AGCT_T
Cuts at: 739 739
Size: 4133
KasI G'GCGC_C
Cuts at: 1563 1811 1563
Size: 248 3885
KpnI G_GTAC'C
Cuts at: 1153 1153
Size: 4133
MluI A'CGCG_T
Cuts at: 3566 3566
Size: 4133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 995 2804 2988 995
Size: 1809 184 2140
Fragments arranged by size:
2140 1809 184
MscI TGG'CCA
Cuts at: 120 1894 120
Size: 1774 2359
MslI CAynn'nnrTG
Cuts at: 2122 2249 2122
Size: 127 4006
MspA1I CmG'CkG
Cuts at: 166 372 507 535 566 1244 1558 1918 2931
Size: 206 135 28 31 678 314 360 1013
Cuts at: 2931 3176 166
Size: 245 1123
Fragments arranged by size:
1123 1013 678 360 314 245 206 135
31 28
NaeI GCC'GGC
Cuts at: 2314 2314
Size: 4133
NarI GG'CG_CC
Cuts at: 1564 1812 1564
Size: 248 3885
NcoI C'CATG_G
Cuts at: 158 158
Size: 4133
NgoMIV G'CCGG_C
Cuts at: 2312 2312
Size: 4133
NspI r_CATG'y
Cuts at: 990 1015 1186 1686 2593 990
Size: 25 171 500 907 2530
Fragments arranged by size:
2530 907 500 171 25
PasI CC'CwG_GG
Cuts at: 672 912 672
Size: 240 3893
PciI A'CATG_T
Cuts at: 2589 2589
Size: 4133
PflMI CCAn_nnn'nTGG
Cuts at: 881 881
Size: 4133
PfoI T'CCnGG_A
Cuts at: 1085 1085
Size: 4133
PmeI GTTT'AAAC
Cuts at: 1132 1132
Size: 4133
PmlI CAC'GTG
Cuts at: 388 388
Size: 4133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3272 3304 3272
Size: 32 4101
PspOMI G'GGCC_C
Cuts at: 276 884 276
Size: 608 3525
PstI C_TGCA'G
Cuts at: 654 666 1180 1865 654
Size: 12 514 685 2922
Fragments arranged by size:
2922 685 514 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4133
PvuII CAG'CTG
Cuts at: 166 507 535 1558 1918 166
Size: 341 28 1023 360 2381
Fragments arranged by size:
2381 1023 360 341 28
RsrII CG'GwC_CG
Cuts at: 2328 2328
Size: 4133
SacI G_AGCT'C
Cuts at: 1147 1147
Size: 4133
SalI G'TCGA_C
Cuts at: 722 1170 722
Size: 448 3685
SapI GCTCTTCn'nnn_
Cuts at: 2156 2366 2156
Size: 210 3923
SbfI CC_TGCA'GG
Cuts at: 654 1180 654
Size: 526 3607
ScaI AGT'ACT
Cuts at: 3650 3650
Size: 4133
SexAI A'CCwGG_T
Cuts at: 492 974 492
Size: 482 3651
SfcI C'TryA_G
Cuts at: 33 650 662 701 795 1176 1861 2854 3045
Size: 617 12 39 94 381 685 993 191
Cuts at: 3045 33
Size: 1121
Fragments arranged by size:
1121 993 685 617 381 191 94 39
12
SfoI GGC'GCC
Cuts at: 1565 1813 1565
Size: 248 3885
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4133
SmaI CCC'GGG
Cuts at: 1155 1155
Size: 4133
SmlI C'TyrA_G
Cuts at: 256 1288 2203 2695 2957 256
Size: 1032 915 492 262 1432
Fragments arranged by size:
1432 1032 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4133
SphI G_CATG'C
Cuts at: 1015 1186 1686 1015
Size: 171 500 3462
Fragments arranged by size:
3462 500 171
StuI AGG'CCT
Cuts at: 627 627
Size: 4133
StyI C'CwwG_G
Cuts at: 158 1267 1621 158
Size: 1109 354 2670
Fragments arranged by size:
2670 1109 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 799 912 940 1716 1849 2308 2517 799
Size: 113 28 776 133 459 209 2415
Fragments arranged by size:
2415 776 459 209 133 113 28
TatI w'GTAC_w
Cuts at: 3648 3648
Size: 4133
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 976 1008 1087 1119 3650 3682 976
Size: 32 79 32 2531 32 1427
Fragments arranged by size:
2531 1427 79 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 976 1930 976
Size: 954 3179
XbaI T'CTAG_A
Cuts at: 1164 1164
Size: 4133
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1628 1628
Size: 4133
XhoI C'TCGA_G
Cuts at: 256 256
Size: 4133
XmaI C'CCGG_G
Cuts at: 1153 1153
Size: 4133
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XmnI
ZraI