Restriction map for
pF1KB9833
(Circular) (Six-base) MAPSORT of: pF1KB9833.seq Check: 9695 from: 1 to: 4148
pF1KB9833 4148 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 279 1186 279
Size: 907 3241
Acc65I G'GTAC_C
Cuts at: 1164 1164
Size: 4148
AclI AA'CG_TT
Cuts at: 1142 3988 1142
Size: 2846 1302
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1973 2405 3152 3420 1973
Size: 432 747 268 2701
Fragments arranged by size:
2701 747 432 268
AflII C'TTAA_G
Cuts at: 214 214
Size: 4148
AflIII A'CryG_T
Cuts at: 177 1500 2604 3581 177
Size: 1323 1104 977 744
Fragments arranged by size:
1323 1104 977 744
AgeI A'CCGG_T
Cuts at: 2536 2536
Size: 4148
AlwNI CAG_nnn'CTG
Cuts at: 1453 3020 1453
Size: 1567 2581
ApaLI G'TGCA_C
Cuts at: 2918 2918
Size: 4148
ApoI r'AATT_y
Cuts at: 135 925 1083 1152 3501 3757 135
Size: 790 158 69 2349 256 526
Fragments arranged by size:
2349 790 526 256 158 69
AseI AT'TA_AT
Cuts at: 20 20
Size: 4148
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
AvaI C'yCGr_G
Cuts at: 1168 1168
Size: 4148
BamHI G'GATC_C
Cuts at: 1173 1173
Size: 4148
BanI G'GyrC_C
Cuts at: 1164 1578 1826 1861 1164
Size: 414 248 35 3451
Fragments arranged by size:
3451 414 248 35
BanII G_rGCy'C
Cuts at: 1162 2192 1162
Size: 1030 3118
BbeI G_GCGC'C
Cuts at: 1582 1830 1582
Size: 248 3900
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4114
BciVI GTATCCnnnnn_n'
Cuts at: 251 2046 2807 4148 251
Size: 1795 761 1341 251
Fragments arranged by size:
1795 1341 761 251
BclI T'GATC_A
Cuts at: 1668 1668
Size: 4148
BglII A'GATC_T
Cuts at: 1663 3700 1663
Size: 2037 2111
BlpI GC'TnA_GC
Cuts at: 1260 1260
Size: 4148
Bme1580I G_kGCm'C
Cuts at: 236 1773 1866 2922 236
Size: 1537 93 1056 1462
Fragments arranged by size:
1537 1462 1056 93
BmgBI CAC'GTC
Cuts at: 178 178
Size: 4148
BmrI ACTGGGnnnn_n'
Cuts at: 298 1360 1441 1776 3920 298
Size: 1062 81 335 2144 526
Fragments arranged by size:
2144 1062 526 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 4148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 994 994
Size: 4148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1324 2203 2695 2993 1324
Size: 879 492 298 2479
Fragments arranged by size:
2479 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3852 3852
Size: 4148
BsaAI yAC'GTr
Cuts at: 447 715 1501 447
Size: 268 786 3094
Fragments arranged by size:
3094 786 268
BsaBI GATnn'nnATC
Cuts at: 6 1662 3699 6
Size: 1656 2037 455
Fragments arranged by size:
2037 1656 455
BsaHI Gr'CG_yC
Cuts at: 1579 1827 2529 4025 1579
Size: 248 702 1496 1702
Fragments arranged by size:
1702 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1337 1858 2536 2810 2957 3368 1337
Size: 521 678 274 147 411 2117
Fragments arranged by size:
2117 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 540 848 1573 2331 2908 3404 540
Size: 308 725 758 577 496 1284
Fragments arranged by size:
1284 758 725 577 496 308
BsiEI CG_ry'CG
Cuts at: 104 1690 1736 2944 104
Size: 1586 46 1208 1308
Fragments arranged by size:
1586 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1162 1940 2130 2922 1162
Size: 778 190 792 2388
Fragments arranged by size:
2388 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 168 168
Size: 4148
Bsp1286I G_dGCh'C
Cuts at: 236 1162 1773 1866 1940 2130 2192 2922 236
Size: 926 611 93 74 190 62 730 1462
Fragments arranged by size:
1462 926 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3368 3368
Size: 4148
BspHI T'CATG_A
Cuts at: 3324 3324
Size: 4148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1198 1412 1714 2095 1198
Size: 214 302 381 3251
Fragments arranged by size:
3251 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2440 2494 3978 4143 50
Size: 2390 54 1484 165 55
Fragments arranged by size:
2390 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2060 2060
Size: 4148
BsrFI r'CCGG_y
Cuts at: 2146 2327 2536 2146
Size: 181 209 3758
Fragments arranged by size:
3758 209 181
BsrGI T'GTAC_A
Cuts at: 155 155
Size: 4148
BssSI C'ACGA_G
Cuts at: 2419 2777 2419
Size: 358 3790
BstAPI GCAn_nnn'nTGC
Cuts at: 1525 1525
Size: 4148
BstBI TT'CG_AA
Cuts at: 2509 2509
Size: 4148
BstYI r'GATC_y
Cuts at: 1 372 1173 1640 1663 1998 2244 3256 3342
Size: 371 801 467 23 335 246 1012 86
Cuts at: 3342 3354 3700 1
Size: 12 346 449
Fragments arranged by size:
1012 801 467 449 371 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 280 280
Size: 4148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 551 2098 2279 2442 551
Size: 1547 181 163 2257
Fragments arranged by size:
2257 1547 181 163
BtsI GCAGTG_nn'
Cuts at: 641 1391 641
Size: 750 3398
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 312 347 312
Size: 35 4113
DraI TTT'AAA
Cuts at: 1147 1147
Size: 4148
DrdI GACnn_nn'nnGTC
Cuts at: 1854 2712 1854
Size: 858 3290
EaeI y'GGCC_r
Cuts at: 693 1733 1907 2298 2325 3670 693
Size: 1040 174 391 27 1345 1171
Fragments arranged by size:
1345 1171 1040 391 174 27
EagI C'GGCC_G
Cuts at: 1733 1733
Size: 4148
EarI CTCTTCn'nnn_
Cuts at: 780 2171 2381 780
Size: 1391 210 2547
Fragments arranged by size:
2547 1391 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2356 2666 2812 3958 2356
Size: 310 146 1146 2546
Fragments arranged by size:
2546 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1160 1160
Size: 4148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 994 1973 2405 3152 3420 994
Size: 979 432 747 268 1722
Fragments arranged by size:
1722 979 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1287 1287
Size: 4148
EcoRI G'AATT_C
Cuts at: 1152 1152
Size: 4148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2167 2167
Size: 4148
FspI TGC'GCA
Cuts at: 1929 1929
Size: 4148
HaeII r_GCGC'y
Cuts at: 1582 1830 2852 3831 1582
Size: 248 1022 979 1899
Fragments arranged by size:
1899 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 529 561 1990 2020 2022 2052 529
Size: 32 1429 30 2 30 2625
Fragments arranged by size:
2625 1429 32 30 30 2
HincII GTy'rAC
Cuts at: 183 1187 183
Size: 1004 3144
HindIII A'AGCT_T
Cuts at: 163 163
Size: 4148
KasI G'GCGC_C
Cuts at: 1578 1826 1578
Size: 248 3900
KpnI G_GTAC'C
Cuts at: 1168 1168
Size: 4148
MluI A'CGCG_T
Cuts at: 3581 3581
Size: 4148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 458 2819 3003 458
Size: 2361 184 1603
Fragments arranged by size:
2361 1603 184
MscI TGG'CCA
Cuts at: 1909 1909
Size: 4148
MslI CAynn'nnrTG
Cuts at: 2137 2264 2137
Size: 127 4021
MspA1I CmG'CkG
Cuts at: 1259 1573 1933 2946 3191 1259
Size: 314 360 1013 245 2216
Fragments arranged by size:
2216 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2329 2329
Size: 4148
NarI GG'CG_CC
Cuts at: 1579 1827 1579
Size: 248 3900
NgoMIV G'CCGG_C
Cuts at: 2327 2327
Size: 4148
NspI r_CATG'y
Cuts at: 1104 1201 1701 2608 1104
Size: 97 500 907 2644
Fragments arranged by size:
2644 907 500 97
PciI A'CATG_T
Cuts at: 2604 2604
Size: 4148
PmeI GTTT'AAAC
Cuts at: 1147 1147
Size: 4148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3287 3319 3287
Size: 32 4116
PstI C_TGCA'G
Cuts at: 1195 1880 1195
Size: 685 3463
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4148
PvuII CAG'CTG
Cuts at: 1573 1933 1573
Size: 360 3788
RsrII CG'GwC_CG
Cuts at: 2343 2343
Size: 4148
SacI G_AGCT'C
Cuts at: 1162 1162
Size: 4148
SalI G'TCGA_C
Cuts at: 1185 1185
Size: 4148
SapI GCTCTTCn'nnn_
Cuts at: 2171 2381 2171
Size: 210 3938
SbfI CC_TGCA'GG
Cuts at: 1195 1195
Size: 4148
ScaI AGT'ACT
Cuts at: 3665 3665
Size: 4148
SfcI C'TryA_G
Cuts at: 33 1191 1876 2869 3060 33
Size: 1158 685 993 191 1121
Fragments arranged by size:
1158 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1580 1828 1580
Size: 248 3900
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
SmaI CCC'GGG
Cuts at: 1170 1170
Size: 4148
SmlI C'TyrA_G
Cuts at: 214 1303 2218 2710 2972 214
Size: 1089 915 492 262 1390
Fragments arranged by size:
1390 1089 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4148
SphI G_CATG'C
Cuts at: 1201 1701 1201
Size: 500 3648
StuI AGG'CCT
Cuts at: 1052 1052
Size: 4148
StyI C'CwwG_G
Cuts at: 920 1282 1636 920
Size: 362 354 3432
Fragments arranged by size:
3432 362 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1731 1864 2323 2532 1731
Size: 133 459 209 3347
Fragments arranged by size:
3347 459 209 133
TatI w'GTAC_w
Cuts at: 155 1013 3663 155
Size: 858 2650 640
Fragments arranged by size:
2650 858 640
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3665 3697 3665
Size: 32 4116
Tth111I GACn'n_nGTC
Cuts at: 1945 1945
Size: 4148
XbaI T'CTAG_A
Cuts at: 1179 1179
Size: 4148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1643 1643
Size: 4148
XmaI C'CCGG_G
Cuts at: 1168 1168
Size: 4148
XmnI GAAnn'nnTTC
Cuts at: 209 209
Size: 4148
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BstZ17I BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmI BssHII BstEII
BstXI Bsu36I BtgI ClaI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI